TY - JOUR AU - Nörenberg, Jasper Maximilian AU - Vida, P. AU - Bösmeier, I. AU - Forró, B. AU - Nörenberg, A. AU - Buda, Á. AU - Simon, Diána AU - Erdő-Bonyár, Szabina AU - Jáksó, Pál AU - Kovács, Kálmán András AU - Mikó, Éva AU - Berki, Tímea AU - Mezősi, Emese AU - Barakonyi, Alíz TI - Decidual γδT cells of early human pregnancy produce angiogenic and immunomodulatory proteins while also possessing cytotoxic potential JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 15 PY - 2024 PG - 12 SN - 1664-3224 DO - 10.3389/fimmu.2024.1382424 UR - https://m2.mtmt.hu/api/publication/34796888 ID - 34796888 N1 - Journal Article; Research Support, Non-U.S. Gov't LA - English DB - MTMT ER - TY - JOUR AU - Gödöny, Krisztina AU - Herczeg, Róbert AU - Gyenesei, Attila AU - Várnagy, Ákos AU - Bognár, Zoltán AU - Kovács, L. Gábor AU - Szekeres, Júlia AU - Mauchart, Péter AU - Nagy, Bernadett AU - Erostyák, János AU - Kovács, Kálmán András AU - Bódis, József TI - Clinical Benefits of Decreased Photo-Oxidative Stress on Human Embryo Development JF - MEDICAL PRINCIPLES AND PRACTICE J2 - MED PRIN PRACT PY - 2024 SN - 1011-7571 DO - 10.1159/000536358 UR - https://m2.mtmt.hu/api/publication/34763514 ID - 34763514 AB - Objective: Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Materials and Methods: We tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control. Results: Blastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate. Conclusions: The implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety. LA - English DB - MTMT ER - TY - JOUR AU - Czétány, Péter AU - Balló, András AU - Márk, László AU - Török, Attila AU - Szántó, Árpád László AU - Máté, Gábor TI - An Alternative Application of Magnetic-Activated Cell Sorting: CD45 and CD235a Based Purification of Semen and Testicular Tissue Samples JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 7 PG - 13 SN - 1661-6596 DO - 10.3390/ijms25073627 UR - https://m2.mtmt.hu/api/publication/34753763 ID - 34753763 AB - Magnetic activated cell sorting (MACS) is a well-known sperm selection technique, which is able to remove apoptotic spermatozoa from semen samples using the classic annexinV based method. Leukocytes and erythrocytes in semen samples or in testicular tissue processed for in vitro fertilization (IVF) could exert detrimental effects on sperm. In the current study, we rethought the aforementioned technique and used magnetic microbeads conjugated with anti-CD45/CD235a antibodies to eliminate contaminating leukocytes and erythrocytes from leukocytospermic semen samples and testicular tissue samples gained via testicular sperm extraction (TESE). With this technique, a 15.7- and a 30.8-fold reduction could be achieved in the ratio of leukocytes in semen and in the number of erythrocytes in TESE samples, respectively. Our results show that MACS is a method worth to reconsider, with more potential alternative applications. Investigations to find molecules labeling high-quality sperm population and the development of positive selection procedures based on these might be a direction of future research. LA - English DB - MTMT ER - TY - JOUR AU - Bokor, Szilvia AU - Csölle, Ildikó AU - Heidinger-Felső, Regina AU - Vass, Réka Anna AU - Funke Kiss, Simone AU - Ertl, Tibor AU - Molnár, Dénes TI - Dietary nutrients during gestation cause obesity and related metabolic changes by altering DNA methylation in the offspring JF - FRONTIERS IN ENDOCRINOLOGY J2 - FRONT ENDOCRINOL VL - 15 PY - 2024 PG - 17 SN - 1664-2392 DO - 10.3389/fendo.2024.1287255 UR - https://m2.mtmt.hu/api/publication/34729560 ID - 34729560 N1 - Department of Paediatrics, Medical School, University of Pécs, Pécs, Hungary National Laboratory on Human Reproduction, University of Pécs, Pécs, Hungary Doctoral School of Health Sciences, Faculty of Health Sciences, University of Pécs, Pécs, Hungary Department of Obstetrics and Gynaecology, Medical School, University of Pécs, Pécs, Hungary Obstetrics and Gynecology, Magyar Imre Hospital Ajka, Ajka, Hungary Export Date: 18 March 2024 Correspondence Address: Bokor, S.; Department of Paediatrics, Hungary; email: bokor.szilvia@pte.hu AB - Growing evidence shows that maternal nutrition from preconception until lactation has an important effect on the development of non-communicable diseases in the offspring. Biological responses to environmental stress during pregnancy, including undernutrition or overnutrition of various nutrients, are transmitted in part by DNA methylation. The aim of the present narrative review is to summarize literature data on altered DNA methylation patterns caused by maternal macronutrient or vitamin intake and its association with offspring's phenotype (obesity and related metabolic changes). With our literature search, we found evidence for the association between alterations in DNA methylation pattern of different genes caused by maternal under- or overnutrition of several nutrients (protein, fructose, fat, vitamin D, methyl-group donor nutrients) during 3 critical periods of programming (preconception, pregnancy, lactation) and the development of obesity or related metabolic changes (glucose, insulin, lipid, leptin, adiponectin levels, blood pressure, non-alcoholic fatty liver disease) in offspring. The review highlights that maternal consumption of several nutrients could individually affect the development of offspring's obesity and related metabolic changes via alterations in DNA methylation. LA - English DB - MTMT ER - TY - CHAP AU - Kovács, R AU - Skriba, Eszter AU - Szmatona, Gábor AU - Tándor, Zoltán AU - Prémusz, Viktória ED - Figler, Mária ED - Tigyi, Zoltánné ED - Karamánné Pakai, Annamária ED - Ungár Tamás Lászlóné Polyák, Éva ED - Kajos, Luca Fanni ED - Trixler, Bettina TI - A fizikai aktivitás összefüggése a mentális egészséggel a BMI tükrében az asszisztált reprodukciós kezelések során T2 - Jubileumi tanulmánykötet: A Pécsi Tudományegyetem Egészségtudományi Kar Egészségtudományi Szakkollégium alapításának 10. évfordulójára PB - Pécsi Tudományegyetem Egészségtudományi Kar (PTE ETK) CY - Pécs SN - 9789636262310 PY - 2024 SP - 227 EP - 241 PG - 15 UR - https://m2.mtmt.hu/api/publication/34724072 ID - 34724072 N1 - A kutatás az Emberi Erőforrások Minisztériumának az ÚNKP-23-4-II-PTE-2061 kódszámú, Új Nemzeti Kiválóság Program és a Nemzeti Kutatási, Fejlesztési és Innovációs Hivatal támogatásával, az NKFI Alapból az NKFI FK-147404 projekten keresztül valósult meg. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Pandur, Edina AU - Pap, Ramóna AU - Jánosa, Gergely AU - Tamási, Kitti AU - Sipos, Katalin TI - Regulation of iron metabolism in HEC‐1A endometrium cells by macrophage‐derived factors and fractalkine JF - CELL BIOLOGY INTERNATIONAL J2 - CELL BIOL INT VL - 48 PY - 2024 IS - 5 SP - 737 EP - 754 PG - 18 SN - 1065-6995 DO - 10.1002/cbin.12144 UR - https://m2.mtmt.hu/api/publication/34691008 ID - 34691008 AB - Macrophages in the endometrium promote receptivity and implantation by secreting proinflammatory cytokines and other factors like fractalkine (FKN). Macrophages are closely linked to regulating iron homeostasis and can modulate iron availability in the tissue microenvironment. It has been revealed that the iron metabolism of the mother is crucial in fertility. Iron metabolism is strictly controlled by hepcidin, the principal iron regulatory protein. The inflammatory cytokines can modulate hepcidin synthesis and, therefore, the iron metabolism of the endometrium. It was proven recently that FKN, a unique chemokine, is implicated in maternal–fetal communication and may contribute to endometrial receptivity and implantation. In the present study, we investigated the effect of activated THP‐1 macrophages and FKN on the iron metabolism of the HEC‐1A endometrial cells. We established a noncontact coculture with or without recombinant human FKN supplementation to study the impact of the macrophage‐derived factors and FKN on the regulation of hepcidin synthesis and iron release and storage of endometrial cells. Based on our findings, the conditioned medium of the activated macrophages could modify hepcidin synthesis via the nuclear factor kappa‐light‐chain‐enhancer of activated B cells, the signal transducer and activator of transcription 3, and the transferrin receptor 2/bone morphogenetic protein 6/suppressor of mothers against decapentaplegic 1/5/8 signaling pathways, and FKN could alter this effect on the endometrial cells. It was also revealed that the conditioned macrophage medium and FKN modulated the iron release and storage of HEC‐1A cells. FKN signaling may be involved in the management of iron trafficking of the endometrium by the regulation of hepcidin. It can contribute to the iron supply for fetal development at the early stage of the pregnancy. LA - English DB - MTMT ER - TY - CONF AU - Pónusz-Kovács, Dalma AU - Kajos, Luca Fanni AU - Pónusz, Róbert AU - Elmer, Diána AU - Csákvári, Tímea AU - Boncz, Imre ED - Csákvári, Tímea ED - Varga, Zoltán TI - Magyarországi egészségbiztosítási kiadások elemzése a leggyakrabban előforduló infertilitás kórképhez kapcsolódóan T2 - VII. Zalaegerszegi nemzetközi egészségturizmus konferencia. Absztrakt kötet PB - Pécsi Tudományegyetem Egészségtudományi Kar (PTE ETK) SN - 9789636262433 PY - 2024 SP - 50. UR - https://m2.mtmt.hu/api/publication/34685271 ID - 34685271 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Rashidiani, Shima AU - Mamo, Gizaw AU - Farkas, Benjámin AU - Szabadi, András AU - Farkas, Bálint AU - Uszkai, Veronika AU - Császár, András AU - Brandt, Barbara AU - Kovács, Kálmán András AU - Pap, Marianna AU - Rauch, Tibor TI - Integrative Epigenetic and Molecular Analysis Reveals a Novel Promoter for a New Isoform of the Transcription Factor TEAD4 JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 4 PG - 15 SN - 1661-6596 DO - 10.3390/ijms25042223 UR - https://m2.mtmt.hu/api/publication/34684525 ID - 34684525 N1 - * Megosztott szerzőség AB - TEAD4 is a transcription factor that plays a crucial role in the Hippo pathway by regulating the expression of genes related to proliferation and apoptosis. It is also involved in the maintenance and differentiation of the trophectoderm during pre- and post-implantation embryonic development. An alternative promoter for the TEAD4 gene was identified through epigenetic profile analysis, and a new transcript from the intronic region of TEAD4 was discovered using the 5'RACE method. The transcript of the novel promoter encodes a TEAD4 isoform (TEAD4-ΔN) that lacks the DNA-binding domain but retains the C-terminal protein-protein interaction domain. Gene expression studies, including end-point PCR and Western blotting, showed that full-length TEAD4 was present in all investigated tissues. However, TEAD4-ΔN was only detectable in certain cell types. The TEAD4-ΔN promoter is conserved throughout evolution and demonstrates transcriptional activity in transient-expression experiments. Our study reveals that TEAD4 interacts with the alternative promoter and increases the expression of the truncated isoform. DNA methylation plays a crucial function in the restricted expression of the TEAD4-ΔN isoform in specific tissues, including the umbilical cord and the placenta. The data presented indicate that the DNA-methylation status of the TEAD4-ΔN promoter plays a critical role in regulating organ size, cancer development, and placenta differentiation. LA - English DB - MTMT ER - TY - CONF AU - Pónusz-Kovács, Dalma AU - Csákvári, Tímea AU - Kajos, Luca Fanni AU - Elmer, Diána AU - Pónusz, Róbert AU - Bódis, József AU - Boncz, Imre ED - Figler, Mária ED - Karamánné Pakai, Annamária ED - Tigyi, Zoltánné ED - Kajos, Luca Fanni ED - Trixler, Bettina TI - Magyarországi területi egyenlőtlenségek elemzése a női infertilitás diagnózis esetében T2 - Jubileumi Szakkollégiumi Konferencia: Absztrakt kötet PB - Pécsi Tudományegyetem Egészségtudományi Kar (PTE ETK) C1 - Pécs SN - 9789636262402 PY - 2024 SP - 20 EP - 20 PG - 1 UR - https://m2.mtmt.hu/api/publication/34667323 ID - 34667323 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Nemeth, Zoltan AU - Schmidt, Roxana AU - Farkas, Bálint TI - Single-incision vaginal mesh insertion for recurrent vaginal vault prolapse after radical cystectomy and radical hysterectomy with irradiation: A case report JF - Continence Reports J2 - Continence Reports VL - 9 PY - 2024 PG - 3 SN - 2772-9745 DO - 10.1016/j.contre.2024.100047 UR - https://m2.mtmt.hu/api/publication/34641519 ID - 34641519 N1 - Case report LA - English DB - MTMT ER -