TY - JOUR
AU - Nörenberg, Jasper Maximilian
AU - Vida, P.
AU - Bösmeier, I.
AU - Forró, B.
AU - Nörenberg, A.
AU - Buda, Á.
AU - Simon, Diána
AU - Erdő-Bonyár, Szabina
AU - Jáksó, Pál
AU - Kovács, Kálmán András
AU - Mikó, Éva
AU - Berki, Tímea
AU - Mezősi, Emese
AU - Barakonyi, Alíz
TI - Decidual γδT cells of early human pregnancy produce angiogenic and immunomodulatory proteins while also possessing cytotoxic potential
JF - FRONTIERS IN IMMUNOLOGY
J2 - FRONT IMMUNOL
VL - 15
PY - 2024
PG - 12
SN - 1664-3224
DO - 10.3389/fimmu.2024.1382424
UR - https://m2.mtmt.hu/api/publication/34796888
ID - 34796888
N1 - Journal Article; Research Support, Non-U.S. Gov't
LA - English
DB - MTMT
ER -
TY - JOUR
AU - Gödöny, Krisztina
AU - Herczeg, Róbert
AU - Gyenesei, Attila
AU - Várnagy, Ákos
AU - Bognár, Zoltán
AU - Kovács, L. Gábor
AU - Szekeres, Júlia
AU - Mauchart, Péter
AU - Nagy, Bernadett
AU - Erostyák, János
AU - Kovács, Kálmán András
AU - Bódis, József
TI - Clinical Benefits of Decreased Photo-Oxidative Stress on Human Embryo Development
JF - MEDICAL PRINCIPLES AND PRACTICE
J2 - MED PRIN PRACT
PY - 2024
SN - 1011-7571
DO - 10.1159/000536358
UR - https://m2.mtmt.hu/api/publication/34763514
ID - 34763514
AB - Objective: Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Materials and Methods: We tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control. Results: Blastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate. Conclusions: The implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety.
LA - English
DB - MTMT
ER -
TY - JOUR
AU - Czétány, Péter
AU - Balló, András
AU - Márk, László
AU - Török, Attila
AU - Szántó, Árpád László
AU - Máté, Gábor
TI - An Alternative Application of Magnetic-Activated Cell Sorting: CD45 and CD235a Based Purification of Semen and Testicular Tissue Samples
JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
J2 - INT J MOL SCI
VL - 25
PY - 2024
IS - 7
PG - 13
SN - 1661-6596
DO - 10.3390/ijms25073627
UR - https://m2.mtmt.hu/api/publication/34753763
ID - 34753763
AB - Magnetic activated cell sorting (MACS) is a well-known sperm selection technique, which is able to remove apoptotic spermatozoa from semen samples using the classic annexinV based method. Leukocytes and erythrocytes in semen samples or in testicular tissue processed for in vitro fertilization (IVF) could exert detrimental effects on sperm. In the current study, we rethought the aforementioned technique and used magnetic microbeads conjugated with anti-CD45/CD235a antibodies to eliminate contaminating leukocytes and erythrocytes from leukocytospermic semen samples and testicular tissue samples gained via testicular sperm extraction (TESE). With this technique, a 15.7- and a 30.8-fold reduction could be achieved in the ratio of leukocytes in semen and in the number of erythrocytes in TESE samples, respectively. Our results show that MACS is a method worth to reconsider, with more potential alternative applications. Investigations to find molecules labeling high-quality sperm population and the development of positive selection procedures based on these might be a direction of future research.
LA - English
DB - MTMT
ER -
TY - JOUR
AU - Bokor, Szilvia
AU - Csölle, Ildikó
AU - Heidinger-Felső, Regina
AU - Vass, Réka Anna
AU - Funke Kiss, Simone
AU - Ertl, Tibor
AU - Molnár, Dénes
TI - Dietary nutrients during gestation cause obesity and related metabolic changes by altering DNA methylation in the offspring
JF - FRONTIERS IN ENDOCRINOLOGY
J2 - FRONT ENDOCRINOL
VL - 15
PY - 2024
PG - 17
SN - 1664-2392
DO - 10.3389/fendo.2024.1287255
UR - https://m2.mtmt.hu/api/publication/34729560
ID - 34729560
N1 - Department of Paediatrics, Medical School, University of Pécs, Pécs, Hungary
National Laboratory on Human Reproduction, University of Pécs, Pécs, Hungary
Doctoral School of Health Sciences, Faculty of Health Sciences, University of Pécs, Pécs, Hungary
Department of Obstetrics and Gynaecology, Medical School, University of Pécs, Pécs, Hungary
Obstetrics and Gynecology, Magyar Imre Hospital Ajka, Ajka, Hungary
Export Date: 18 March 2024
Correspondence Address: Bokor, S.; Department of Paediatrics, Hungary; email: bokor.szilvia@pte.hu
AB - Growing evidence shows that maternal nutrition from preconception until lactation has an important effect on the development of non-communicable diseases in the offspring. Biological responses to environmental stress during pregnancy, including undernutrition or overnutrition of various nutrients, are transmitted in part by DNA methylation. The aim of the present narrative review is to summarize literature data on altered DNA methylation patterns caused by maternal macronutrient or vitamin intake and its association with offspring's phenotype (obesity and related metabolic changes). With our literature search, we found evidence for the association between alterations in DNA methylation pattern of different genes caused by maternal under- or overnutrition of several nutrients (protein, fructose, fat, vitamin D, methyl-group donor nutrients) during 3 critical periods of programming (preconception, pregnancy, lactation) and the development of obesity or related metabolic changes (glucose, insulin, lipid, leptin, adiponectin levels, blood pressure, non-alcoholic fatty liver disease) in offspring. The review highlights that maternal consumption of several nutrients could individually affect the development of offspring's obesity and related metabolic changes via alterations in DNA methylation.
LA - English
DB - MTMT
ER -
TY - CHAP
AU - Kovács, R
AU - Skriba, Eszter
AU - Szmatona, Gábor
AU - Tándor, Zoltán
AU - Prémusz, Viktória
ED - Figler, Mária
ED - Tigyi, Zoltánné
ED - Karamánné Pakai, Annamária
ED - Ungár Tamás Lászlóné Polyák, Éva
ED - Kajos, Luca Fanni
ED - Trixler, Bettina
TI - A fizikai aktivitás összefüggése a mentális egészséggel a BMI tükrében az asszisztált reprodukciós kezelések során
T2 - Jubileumi tanulmánykötet: A Pécsi Tudományegyetem Egészségtudományi Kar Egészségtudományi Szakkollégium alapításának 10. évfordulójára
PB - Pécsi Tudományegyetem Egészségtudományi Kar (PTE ETK)
CY - Pécs
SN - 9789636262310
PY - 2024
SP - 227
EP - 241
PG - 15
UR - https://m2.mtmt.hu/api/publication/34724072
ID - 34724072
N1 - A kutatás az Emberi Erőforrások Minisztériumának az ÚNKP-23-4-II-PTE-2061 kódszámú, Új Nemzeti Kiválóság Program és a Nemzeti Kutatási, Fejlesztési és Innovációs Hivatal támogatásával, az NKFI Alapból az NKFI FK-147404 projekten keresztül valósult meg.
LA - Hungarian
DB - MTMT
ER -
TY - JOUR
AU - Pandur, Edina
AU - Pap, Ramóna
AU - Jánosa, Gergely
AU - Tamási, Kitti
AU - Sipos, Katalin
TI - Regulation of iron metabolism in HEC‐1A endometrium cells by macrophage‐derived factors and fractalkine
JF - CELL BIOLOGY INTERNATIONAL
J2 - CELL BIOL INT
VL - 48
PY - 2024
IS - 5
SP - 737
EP - 754
PG - 18
SN - 1065-6995
DO - 10.1002/cbin.12144
UR - https://m2.mtmt.hu/api/publication/34691008
ID - 34691008
AB - Macrophages in the endometrium promote receptivity and implantation by secreting proinflammatory cytokines and other factors like fractalkine (FKN). Macrophages are closely linked to regulating iron homeostasis and can modulate iron availability in the tissue microenvironment. It has been revealed that the iron metabolism of the mother is crucial in fertility. Iron metabolism is strictly controlled by hepcidin, the principal iron regulatory protein. The inflammatory cytokines can modulate hepcidin synthesis and, therefore, the iron metabolism of the endometrium. It was proven recently that FKN, a unique chemokine, is implicated in maternal–fetal communication and may contribute to endometrial receptivity and implantation. In the present study, we investigated the effect of activated THP‐1 macrophages and FKN on the iron metabolism of the HEC‐1A endometrial cells. We established a noncontact coculture with or without recombinant human FKN supplementation to study the impact of the macrophage‐derived factors and FKN on the regulation of hepcidin synthesis and iron release and storage of endometrial cells. Based on our findings, the conditioned medium of the activated macrophages could modify hepcidin synthesis via the nuclear factor kappa‐light‐chain‐enhancer of activated B cells, the signal transducer and activator of transcription 3, and the transferrin receptor 2/bone morphogenetic protein 6/suppressor of mothers against decapentaplegic 1/5/8 signaling pathways, and FKN could alter this effect on the endometrial cells. It was also revealed that the conditioned macrophage medium and FKN modulated the iron release and storage of HEC‐1A cells. FKN signaling may be involved in the management of iron trafficking of the endometrium by the regulation of hepcidin. It can contribute to the iron supply for fetal development at the early stage of the pregnancy.
LA - English
DB - MTMT
ER -
TY - CONF
AU - Pónusz-Kovács, Dalma
AU - Kajos, Luca Fanni
AU - Pónusz, Róbert
AU - Elmer, Diána
AU - Csákvári, Tímea
AU - Boncz, Imre
ED - Csákvári, Tímea
ED - Varga, Zoltán
TI - Magyarországi egészségbiztosítási kiadások elemzése a leggyakrabban előforduló infertilitás kórképhez kapcsolódóan
T2 - VII. Zalaegerszegi nemzetközi egészségturizmus konferencia. Absztrakt kötet
PB - Pécsi Tudományegyetem Egészségtudományi Kar (PTE ETK)
SN - 9789636262433
PY - 2024
SP - 50.
UR - https://m2.mtmt.hu/api/publication/34685271
ID - 34685271
LA - Hungarian
DB - MTMT
ER -
TY - JOUR
AU - Rashidiani, Shima
AU - Mamo, Gizaw
AU - Farkas, Benjámin
AU - Szabadi, András
AU - Farkas, Bálint
AU - Uszkai, Veronika
AU - Császár, András
AU - Brandt, Barbara
AU - Kovács, Kálmán András
AU - Pap, Marianna
AU - Rauch, Tibor
TI - Integrative Epigenetic and Molecular Analysis Reveals a Novel Promoter for a New Isoform of the Transcription Factor TEAD4
JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
J2 - INT J MOL SCI
VL - 25
PY - 2024
IS - 4
PG - 15
SN - 1661-6596
DO - 10.3390/ijms25042223
UR - https://m2.mtmt.hu/api/publication/34684525
ID - 34684525
N1 - * Megosztott szerzőség
AB - TEAD4 is a transcription factor that plays a crucial role in the Hippo pathway by regulating the expression of genes related to proliferation and apoptosis. It is also involved in the maintenance and differentiation of the trophectoderm during pre- and post-implantation embryonic development. An alternative promoter for the TEAD4 gene was identified through epigenetic profile analysis, and a new transcript from the intronic region of TEAD4 was discovered using the 5'RACE method. The transcript of the novel promoter encodes a TEAD4 isoform (TEAD4-ΔN) that lacks the DNA-binding domain but retains the C-terminal protein-protein interaction domain. Gene expression studies, including end-point PCR and Western blotting, showed that full-length TEAD4 was present in all investigated tissues. However, TEAD4-ΔN was only detectable in certain cell types. The TEAD4-ΔN promoter is conserved throughout evolution and demonstrates transcriptional activity in transient-expression experiments. Our study reveals that TEAD4 interacts with the alternative promoter and increases the expression of the truncated isoform. DNA methylation plays a crucial function in the restricted expression of the TEAD4-ΔN isoform in specific tissues, including the umbilical cord and the placenta. The data presented indicate that the DNA-methylation status of the TEAD4-ΔN promoter plays a critical role in regulating organ size, cancer development, and placenta differentiation.
LA - English
DB - MTMT
ER -
TY - CONF
AU - Pónusz-Kovács, Dalma
AU - Csákvári, Tímea
AU - Kajos, Luca Fanni
AU - Elmer, Diána
AU - Pónusz, Róbert
AU - Bódis, József
AU - Boncz, Imre
ED - Figler, Mária
ED - Karamánné Pakai, Annamária
ED - Tigyi, Zoltánné
ED - Kajos, Luca Fanni
ED - Trixler, Bettina
TI - Magyarországi területi egyenlőtlenségek elemzése a női infertilitás diagnózis esetében
T2 - Jubileumi Szakkollégiumi Konferencia: Absztrakt kötet
PB - Pécsi Tudományegyetem Egészségtudományi Kar (PTE ETK)
C1 - Pécs
SN - 9789636262402
PY - 2024
SP - 20
EP - 20
PG - 1
UR - https://m2.mtmt.hu/api/publication/34667323
ID - 34667323
LA - Hungarian
DB - MTMT
ER -
TY - JOUR
AU - Nemeth, Zoltan
AU - Schmidt, Roxana
AU - Farkas, Bálint
TI - Single-incision vaginal mesh insertion for recurrent vaginal vault prolapse after radical cystectomy and radical hysterectomy with irradiation: A case report
JF - Continence Reports
J2 - Continence Reports
VL - 9
PY - 2024
PG - 3
SN - 2772-9745
DO - 10.1016/j.contre.2024.100047
UR - https://m2.mtmt.hu/api/publication/34641519
ID - 34641519
N1 - Case report
LA - English
DB - MTMT
ER -