TY - JOUR AU - Farkasinszky, Gergely AU - Péliné Szabó, Judit AU - Károlyi, Péter AU - Rácz, Szilvia AU - Dénes, Noémi AU - Papp, Tamás AU - Király, József AU - Szabó, Zsuzsanna AU - Kertész, István AU - Mező, Gábor AU - Halmos, Gábor AU - Képes, Zita AU - Trencsényi, György TI - In Vivo Imaging of Acute Hindlimb Ischaemia in Rat Model: A Pre-Clinical PET Study JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 16 PY - 2024 IS - 4 SP - 542 SN - 1999-4923 DO - 10.3390/pharmaceutics16040542 UR - https://m2.mtmt.hu/api/publication/34820368 ID - 34820368 AB - Background: To better understand ischaemia-related molecular alterations, temporal changes in angiogenic Aminopeptidase N (APN/CD13) expression and glucose metabolism were assessed with PET using a rat model of peripheral arterial disease (PAD). Methods: The mechanical occlusion of the base of the left hindlimb triggered using a tourniquet was applied to establish the ischaemia/reperfusion injury model in Fischer-344 rats. 2-[18F]FDG and [68Ga]Ga-NOTA-c(NGR) PET imaging performed 1, 3, 5, 7, and 10 days post-ischaemia induction was followed by Western blotting and immunohistochemical staining for APN/CD13 in ischaemic and control muscle tissue extracts. Results: Due to a cellular adaptation to hypoxia, a gradual increase in [68Ga]Ga-NOTA-c(NGR) and 2-[18F]FDG uptake was observed from post-intervention day 1 to 7 in the ischaemic hindlimbs, which was followed by a drop on day 10. Conforming pronounced angiogenic recovery, the NGR accretion of the ischaemic extremities differed significantly from the controls 5, 7, and 10 days after ischaemia induction (p ≤ 0.05), which correlated with the Western blot and immunohistochemical results. No remarkable radioactivity was depicted between the normally perfused hindlimbs of either the ischaemic or the control groups. Conclusions: The PET-based longitudinal assessment of angiogenesis-associated APN/CD13 expression and glucose metabolism during ischaemia may continue to broaden our knowledge on the pathophysiology of PAD. LA - English DB - MTMT ER - TY - JOUR AU - Bíró, Krisztina AU - Ujhelyi, Zoltán AU - Schlammadinger, Ágota AU - Rázsó, Katalin AU - Buchholcz, Gyula AU - Boda, Zoltán TI - Antitrombin-III-deficites gravidák - kezelési lehetőségek JF - GYÓGYSZERÉSZET J2 - GYÓGYSZERÉSZET VL - 68 PY - 2024 IS - 2 SP - 62 EP - 66 PG - 5 SN - 0017-6036 UR - https://m2.mtmt.hu/api/publication/34813506 ID - 34813506 LA - Hungarian DB - MTMT ER - TY - CONF AU - Fésüs, Adina AU - Matuz, M AU - Hambalek, H AU - Ruzsa, R AU - Tánczos, B AU - Bácskay, I AU - Illés, Á AU - Benkő, R TI - 4CPS-036 Evaluation of the diagnosis and antibiotic prescription pattern in patients hospitalised with urinary tract infections (UTIs) T2 - Section 4: Clinical pharmacy services PB - British Medical Journal Publishing Group PY - 2024 SP - A68.2 EP - A68 DO - 10.1136/ejhpharm-2024-eahp.140 UR - https://m2.mtmt.hu/api/publication/34756237 ID - 34756237 LA - English DB - MTMT ER - TY - CONF AU - Fésüs, Adina AU - Baluku, P AU - Sipos, É AU - Somodi, S AU - Vaskó, A AU - Lekli, I AU - Berczi-Kun, E AU - Bácskay, I TI - 4CPS-035 Impact of antibiotic stewardship programme (ASP) on antibiotic use and clinical outcomes in patients hospitalised with community-acquired pneumonia (CAP): retrospective observational before-after study T2 - Section 4: Clinical pharmacy services PB - British Medical Journal Publishing Group PY - 2024 SP - A68.1 EP - A68 DO - 10.1136/ejhpharm-2024-eahp.139 UR - https://m2.mtmt.hu/api/publication/34756232 ID - 34756232 LA - English DB - MTMT ER - TY - CONF AU - Imre, Alexandra AU - Kovács, Renátó AU - Ibrahim, Al’ Abri AU - Nathan, Crook AU - Pfliegler, Valter Péter TI - Specific high effect mutations in clinical and experimentally evolved Saccharomyces ‘boulardii’ isolates show that genes involved in chemical response might have a role during the adaptation to the human host T2 - The Allied Genetics Conference 2024 Abstract Book PY - 2024 UR - https://m2.mtmt.hu/api/publication/34753304 ID - 34753304 LA - English DB - MTMT ER - TY - JOUR AU - Székvölgyi, Lóránt TI - Chromosomal R-loops: who R they? JF - BIOLOGIA FUTURA J2 - BIOL FUTURA VL - 3 PY - 2024 SP - 1 EP - 8 PG - 8 SN - 2676-8615 DO - 10.1007/s42977-024-00213-7 UR - https://m2.mtmt.hu/api/publication/34735372 ID - 34735372 AB - R-loops, composed of DNA–RNA hybrids and displaced single-stranded DNA, are known to pose a severe threat to genome integrity. Therefore, extensive research has focused on identifying regulatory proteins involved in controlling R-loop levels. These proteins play critical roles in preventing R-loop accumulation and associated genome instability. Herein I summarize recent knowledge on R-loop regulators affecting R-loop homeostasis, involving a wide array of R-loop screening methods that have enabled their characterization, from forward genetic and siRNA-based screens to proximity labeling and machine learning. These approaches not only deepen our understanding on R-loop formation processes, but also hold promise to find new targets in R-loop dysregulation associated with human pathologies. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Balázs István AU - Bahar, Bazeli AU - Annelies, Janssens AU - Lisztes, Erika AU - Racskó, Márk AU - Kelemen, Balázs AU - Herczeg, Mihály AU - Nagy, Tamás Milán AU - E Kövér, Katalin AU - Argha, Mitra AU - Attila, Borics AU - Bíró, Tamás AU - Thomas, Voets TI - Direct modulation of TRPM8 ion channels by rapamycin and analog macrolide immunosuppressants JF - ELIFE J2 - ELIFE PY - 2024 SN - 2050-084X UR - https://m2.mtmt.hu/api/publication/34722559 ID - 34722559 N1 - preprint DOI-ja: 10.1101/2024.02.01.578392 LA - English DB - MTMT ER - TY - JOUR AU - Ababneh, Haneen AU - Balogh, Enikő AU - Csiki, Dávid Máté AU - Lente, Gréta AU - Fenyvesi, Ferenc AU - Tóth, Andrea AU - Jeney, Viktória TI - High glucose promotes osteogenic differentiation of human lens epithelial cells through hypoxia-inducible factor (HIF) activation JF - JOURNAL OF CELLULAR PHYSIOLOGY J2 - J CELL PHYSIOL PY - 2024 PG - 12 SN - 0021-9541 DO - 10.1002/jcp.31211 UR - https://m2.mtmt.hu/api/publication/34618976 ID - 34618976 AB - Cataract, a leading cause of blindness, is characterised by lens opacification. Type 2 diabetes is associated with a two- to fivefold higher prevalence of cataracts. The risk of cataract formation increases with the duration of diabetes and the severity of hyperglycaemia. Hydroxyapatite deposition is present in cataractous lenses that could be the consequence of osteogenic differentiation and calcification of lens epithelial cells (LECs). We hypothesised that hyperglycaemia might promote the osteogenic differentiation of human LECs (HuLECs). Osteogenic medium (OM) containing excess phosphate and calcium with normal (1 g/L) or high (4.5 g/L) glucose was used to induce HuLEC calcification. High glucose accelerated and intensified OM-induced calcification of HuLECs, which was accompanied by hyperglycaemia-induced upregulation of the osteogenic markers Runx2, Sox9, alkaline phosphatase and osteocalcin, as well as nuclear translocation of Runx2. High glucose-induced calcification was abolished in Runx2-deficient HuLECs. Additionally, high glucose stabilised the regulatory alpha subunits of hypoxia-inducible factor 1 (HIF-1), triggered nuclear translocation of HIF-1 alpha and increased the expression of HIF-1 target genes. Gene silencing of HIF-1 alpha or HIF-2 alpha attenuated hyperglycaemia-induced calcification of HuLECs, while hypoxia mimetics (desferrioxamine, CoCl2) enhanced calcification of HuLECs under normal glucose conditions. Overall, this study suggests that high glucose promotes HuLEC calcification via Runx2 and the activation of the HIF-1 signalling pathway. These findings may provide new insights into the pathogenesis of diabetic cataracts, shedding light on potential factors for intervention to treat this sight-threatening condition. LA - English DB - MTMT ER - TY - JOUR AU - Tánczos, Bence AU - Vass, Virág AU - Szabó, Erzsébet AU - Lovas, Miklós AU - Kattoub, Rasha Ghanem AU - Bakai-Bereczki, Ilona AU - Borbás, Anikó AU - Herczegh, Pál AU - Tósaki, Árpád TI - Effects of H2S-donor ascorbic acid derivative and ischemia/reperfusion-induced injury in isolated rat hearts JF - EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES J2 - EUR J PHARM SCI VL - 195 PY - 2024 IS - - SP - 106721 SN - 0928-0987 DO - 10.1016/j.ejps.2024.106721 UR - https://m2.mtmt.hu/api/publication/34572686 ID - 34572686 LA - English DB - MTMT ER - TY - JOUR AU - Ratku, Balázs AU - Lőrincz, Hajnalka AU - Bak-Csiha, Sára AU - Sebestyén, Veronika AU - Berta, Eszter AU - Bodor, Miklós AU - Nagy, Endre AU - Szabó, Zoltán AU - Harangi, Mariann AU - Somodi, Sándor TI - Serum afamin and its implications in adult growth hormone deficiency: a prospective GH-withdrawal study JF - FRONTIERS IN ENDOCRINOLOGY J2 - FRONT ENDOCRINOL VL - 15 PY - 2024 SN - 1664-2392 DO - 10.3389/fendo.2024.1348046 UR - https://m2.mtmt.hu/api/publication/34564621 ID - 34564621 LA - English DB - MTMT ER -