TY - JOUR AU - Feberwee, Anneke AU - Ferguson-Noel, Naola AU - Catania, Salvatore AU - Bottinelli, Marco AU - Wawagema, Nadeeka AU - Gyuranecz, Miklós AU - Gautier-Bouchardon, Anne V AU - Lysnyansky, Inna AU - Wiegel, Jeanine AU - Palau-Ribes, Franca Möller AU - Ramirez, Ana S. TI - Mycoplasma gallisepticum and Mycoplasma synoviae in commercial poultry: Current control strategies and future challenges JF - AVIAN PATHOLOGY J2 - AVIAN PATHOL VL - 54 PY - 2025 IS - 2 SP - 168 EP - 174 PG - 7 SN - 0307-9457 DO - 10.1080/03079457.2024.2419037 UR - https://m2.mtmt.hu/api/publication/35497845 ID - 35497845 N1 - Royal GD, Deventer, Netherlands Poultry Diagnostic and Research Center, The University of Georgia, Athens, GA, United States Istituto Zooprofilattico Sperimentale delle Venezie, Buttapietra, Italy Melbourne Veterinary School, University of Melbourne, Melbourne, Australia HUN-REN Veterinary Medical Research Institute, Budapest, Hungary Mycoplasmology, Bacteriology and AMR Unit, ANSES, Ploufragan, France Kimron Veterinary Institute, Beit Dagan, Israel Clinic for Birds, Reptiles, Amphibians and Fish, Justus Liebig University Giessen, Giessen, Germany Universidad de Las Palmas de Gran Canaria, Canary Islands, Spain Export Date: 14 March 2025; Cited By: 1; Correspondence Address: A. Feberwee; Royal GD, Deventer, Netherlands; email: a.feberwee@gddiergezondheid.nl; CODEN: AVPAD LA - English DB - MTMT ER - TY - JOUR AU - Klein, Ulrich AU - Földi, Dorottya AU - Nagy, Eszter Zsófia AU - Tóth, Lilla AU - Belecz, Nikolett AU - Költő, Karola AU - Wehmann, Enikő AU - Marton, Szilvia AU - Merenda, Marianna AU - Gastaldelli, Michele AU - Catania, Salvatore AU - Spergser, Joachim AU - Siesenop, Ute AU - Vyt, Philip AU - Bányai, Krisztián AU - Kreizinger, Zsuzsa AU - Depondt, Wouter AU - Gyuranecz, Miklós TI - Antimicrobial susceptibility profiles of Mycoplasma hyosynoviae strains isolated from five European countries between 2018 and 2023 JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 15 PY - 2025 IS - 1 SN - 2045-2322 DO - 10.1038/s41598-024-85052-1 UR - https://m2.mtmt.hu/api/publication/35675746 ID - 35675746 N1 - Huvepharma NV, Antwerp, Belgium HUN-REN Veterinary Medical Research Institute, Budapest, Hungary National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, Hungary Mycoplasma Unit, Istituto Zooprofilattico Sperimentale Della Venezie, VR, Buttapietra, Italy Institute of Microbiology, University of Veterinary Medicine, Vienna, Austria Institute for Microbiology, University of Veterinary Medicine Hannover, Hannover, Germany Dialab Diagnostic Laboratory, Belsele, Belgium University of Veterinary Medicine, Budapest, Hungary MolliScience Kft., Biatorbágy, Hungary Export Date: 31 January 2025 Correspondence Address: Klein, U.; Huvepharma NVBelgium; email: ulrich.klein@huvepharma.com Correspondence Address: Gyuranecz, M.; HUN-REN Veterinary Medical Research InstituteHungary; email: m.gyuranecz@gmail.com AB - Mycoplasma ( M .) hyosynoviae is a facultative pathogen, causing arthritis in finisher pigs world-wide. In the absence of a commercial vaccine improvement of housing conditions and antibiotic therapy are the only options to alleviate the clinical signs. This study aimed to determine antibiotic susceptibility profiles of 106 M . hyosynoviae isolates against ten antibiotics licensed for veterinary use in cases of arthritis. The isolates were collected between 2018 and 2023 from five European countries: Austria (n = 20), Belgium (n = 20), Germany (n = 25), Hungary (n = 21) and Italy (n = 20). The minimal inhibitory concentrations (MIC) were determined by broth micro-dilution assay. The tested isolates were highly susceptible to tiamulin (MIC 90 ≤ 0.039 µg/ml), tylvalosin (MIC 90 ≤ 0.039 µg/ml) and lincomycin (MIC 90 ≤ 0.25 µg/ml). Low concentrations of tylosin (MIC 90 0.5 µg/ml) and tilmicosin (MIC 90 1 µg/ml) inhibited the growth of the isolates. While moderate minimal inhibitory concentrations were detected for doxycycline (MIC 90 0.312 µg/ml), oxytetracycline (MIC 90 2 µg/ml), enrofloxacin (MIC 90 0.625 µg/ml) and florfenicol (MIC 90 2 µg/ml), only high concentrations of tulathromycin (MIC 90 64 µg/ml) inhibited the growth of the isolates. Statistical analysis revealed significant differences between countries in case of enrofloxacin, where the Hungarian isolates showed the lowest MIC values, and the German isolates the highest MIC values among the tested countries. Our results show that European M. hyosynoviae isolates are generally susceptible to the tested antibiotics with the exception of tulathromycin. The country specific differences indicate the importance of regular susceptibility testing of isolates on a Pan-European level. LA - English DB - MTMT ER - TY - JOUR AU - Homonnay, Zalán Gábor AU - Jakab, Szilvia AU - Marton, Szilvia AU - Domán, Marianna AU - Bali, Krisztina AU - Kaszab, Eszter AU - Kemenesi, Gábor AU - Mató, Tamás AU - Kiss, István AU - Palya, Vilmos AU - Bányai, Krisztián TI - Genomic Mosaicism in Fowl Adenovirus 3 Strains JF - ANIMALS J2 - ANIMALS-BASEL VL - 15 PY - 2025 IS - 4 PG - 10 SN - 2076-2615 DO - 10.3390/ani15040508 UR - https://m2.mtmt.hu/api/publication/35760835 ID - 35760835 N1 - * Megosztott szerzőség AB - Fowl adenovirus D is the main cause of inclusion body hepatitis in chickens. Whole genome sequencing was carried out to enrich the genomic sequence database using field isolates of FAdV-D. Out of 44 newly determined genomes, 43 were classified into FAdV-2/-11 and 2 into FAdV-3; no FAdV-9 was identified. Whole-genome based phylogeny showed that FAdV-3 was more distantly related to FAdV 9 and FAdV-2/-11 than FAdV-9 and FAdV-2/-11 to each other. Whole-genome sequence homology analysis revealed that the full-length FAdV-3 genome harbored a ~12 kbp fragment of the genome that shared moderate sequence homology with representative strains of other FAdV-D serotypes but high relatedness with only the FAdV-3 strain whose full-genome is available in GenBank. A closer look onto the fiber and the penton genes of our FAdV-3 isolate identified putative recombination events; both the fiber and the penton coding genes shared fragments originating from FAdV-9. Of interest, ORF19 displayed a close relationship with the homologous genomic region of some FAdV-E strains (amino acid sequence homology, up to 82%). Thus, although FAdV-3 is classified into FAdV-D, the genomic structure of FAdV-3 appears to result from multiple heterotypic and heterologous recombination events. This study highlights the unique origin of FAdV-3. LA - English DB - MTMT ER - TY - JOUR AU - Görföl, Tamás AU - Tóth, Gábor Endre AU - Tu, Vuong Tan AU - Lanszki, Zsófia AU - Kaszab, Eszter AU - Bali, Krisztina AU - Bányai, Krisztián AU - Kemenesi, Gábor TI - Metagenomic detection of a wide variety of viruses from Vietnamese bats JF - INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES J2 - INT J INFECT DIS VL - 152 PY - 2025 PG - 1 SN - 1201-9712 DO - 10.1016/j.ijid.2024.107659 UR - https://m2.mtmt.hu/api/publication/35803173 ID - 35803173 LA - English DB - MTMT ER - TY - JOUR AU - Nagy, Eszter Zsófia AU - Földi, Dorottya AU - Madzig, Fruzsina AU - Wehmann, Enikő AU - Orosz, Adél AU - Kempf, András AU - Buza, László AU - Mátyus, János AU - Búza, László AU - Grózner, Dénes AU - Kreizinger, Zsuzsa AU - Gyuranecz, Miklós TI - Cross-sectional study of Mycoplasma hyopharyngis, Mycoplasma hyopneumoniae, Mycoplasma hyorhinis and Mycoplasma hyosynoviae in the tonsils of fattening pigs from Central-Eastern Europe JF - PORCINE HEALTH MANAGEMENT J2 - PORCINE HEALTH MANAG VL - 11 PY - 2025 IS - 1 SN - 2055-5660 DO - 10.1186/s40813-025-00429-6 UR - https://m2.mtmt.hu/api/publication/35812828 ID - 35812828 LA - English DB - MTMT ER - TY - PAT AU - Gyuranecz, Miklós AU - Nagy, Eszter Zsófia TI - Mycoplasma hyorhinis törzs CY - Country:10001(1) PY - 2025 UR - https://m2.mtmt.hu/api/publication/35892860 ID - 35892860 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Nagy, Eszter Zsófia AU - Szeredi, Levente AU - Muramatsu, Hiromi AU - Nagy, Noémi AU - Grózner, Dénes AU - Kreizinger, Zsuzsa AU - Sulyok, Kinga Mária AU - Pangabam, Bandana AU - Tóth, Lilla AU - Jun, Rachel H.J. AU - Földi, Dorottya AU - Wehmann, Enikő AU - Tenk, Miklós AU - Pardi, Norbert AU - Gyuranecz, Miklós TI - Efficacy test of a Mycoplasma hyorhinis mRNA-LNP vaccine candidate JF - VACCINE: X J2 - VACCINE X VL - 25 PY - 2025 SN - 2590-1362 DO - 10.1016/j.jvacx.2025.100684 UR - https://m2.mtmt.hu/api/publication/36223974 ID - 36223974 LA - English DB - MTMT ER - TY - JOUR AU - Fehér, Enikő AU - Kaszab, Eszter AU - Mótyán, János András AU - Máté, Dóra AU - Bali, Krisztina AU - Hoitsy, Márton György AU - Sós, Endre AU - Jakab, Ferenc AU - Bányai, Krisztián TI - Structural similarity of human papillomavirus E4 and polyomaviral VP4 exhibited by genomic analysis of the common kestrel (Falco tinnunculus) polyomavirus JF - VETERINARY RESEARCH COMMUNICATIONS J2 - VET RES COMMUN VL - 48 PY - 2024 IS - 1 SP - 309 EP - 315 PG - 7 SN - 0165-7380 DO - 10.1007/s11259-023-10210-1 UR - https://m2.mtmt.hu/api/publication/34135669 ID - 34135669 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Fund [TKP2021-EGA-01]; National Laboratory for Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health, and Food Chain Safety [RRF-2.3.1-21-2022-00001]; National Research, Development and Innovation Fund of Hungary [TKP2021-NVA-07, TKP2021-NVA]; National Laboratory of Virology [RRF-2.3.1-21-2022-00010] Funding text: This research has been implemented with the support provided by the Ministry of Innovation and Technology of Hungary (legal successor: Ministry of Culture and Innovation of Hungary) from the National Research, Development and Innovation Fund, financed under the TKP2021-EGA-01 funding scheme of the National Research, Development and Innovation Office. Additional support was provided by the National Laboratory for Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health, and Food Chain Safety, RRF-2.3.1-21-2022-00001. Project no. TKP2021-NVA-07 has been implemented with the support provided from the National Research, Development and Innovation Fund of Hungary, financed under the TKP2021-NVA funding scheme. This work was supported by the National Laboratory of Virology, project no. RRF-2.3.1-21-2022-00010. AB - Polyomaviruses are widely distributed viruses of birds that may induce developmental deformities and internal organ disorders primarily in nestlings. In this study, polyomavirus sequence was detected in kidney and liver samples of a common kestrel ( Falco tinnunculus ) that succumbed at a rescue station in Hungary. The amplified 5025 nucleotide (nt) long genome contained the early (large and small T antigen, LTA and STA) and late (viral proteins, VP1, VP2, VP3) open reading frames (ORFs) typical for polyomaviruses. One of the additional putative ORFs (named VP4) showed identical localization with the VP4 and ORF-X of gammapolyomaviruses, but putative splicing sites could not be found in its sequence. Interestingly, the predicted 123 amino acid (aa) long protein sequence showed the highest similarity with human papillomavirus E4 early proteins in respect of the aa distribution and motif arrangement implying similar functions. The LTA of the kestrel polyomavirus shared <59.2% nt and aa pairwise identity with the LTA sequence of other polyomaviruses and formed a separated branch in the phylogenetic tree among gammapolyomaviruses. Accordingly, the kestrel polyomavirus may be the first member of a novel species within the Gammapolyomavirus genus, tentatively named Gammapolyomavirus faltin . LA - English DB - MTMT ER - TY - JOUR AU - Araujo Neto, José Pompeu AU - Székely, Csaba AU - Molnár, Kálmán AU - Pereira, Camila Maria Barbosa AU - Guerreiro, Sávio Lucas de Matos AU - Hamoy, Igor G. AU - Matos, Edilson R. TI - Morphology and phylogeny of Coccomyxa bragantinensis n. sp. (Cnidaria: Myxozoa) found parasitising the Coco Sea catfish, Bagre bagre (Siluriformes: Ariidae), captured off the coast of Northern Brazil JF - PARASITOLOGY INTERNATIONAL J2 - PARASITOL INT VL - 98 PY - 2024 PG - 6 SN - 1383-5769 DO - 10.1016/j.parint.2023.102815 UR - https://m2.mtmt.hu/api/publication/34220321 ID - 34220321 LA - English DB - MTMT ER - TY - JOUR AU - Jakab, Szilvia AU - Bálint, Ádám AU - Cseri, Karolina AU - Bali, Krisztina AU - Kaszab, Eszter AU - Domán, Marianna AU - Halas, Máté AU - Szarka, Krisztina Zsuzsanna AU - Bányai, Krisztián TI - Genome stability assessment of PRRS vaccine strain with new ARTIC-style sequencing protocol JF - FRONTIERS IN VETERINARY SCIENCE J2 - FRONT VET SCI VL - 10 PY - 2024 SN - 2297-1769 DO - 10.3389/fvets.2023.1327725 UR - https://m2.mtmt.hu/api/publication/34483784 ID - 34483784 N1 - Pathogen Discovery Group, HUN-REN Veterinary Medical Research Institute, Budapest, Hungary National Laboratory for Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, Budapest, Hungary Veterinary Diagnostic Directorate, National Food Chain Safety Office, Budapest, Hungary One Health Institute, University of Debrecen, Debrecen, Hungary Department of Metagenomics, University of Debrecen, Debrecen, Hungary Prophyl Ltd., Mohács, Hungary Department of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, Hungary Export Date: 4 February 2025 Correspondence Address: Bányai, K.; Pathogen Discovery Group, Hungary AB - A tiling amplicon sequencing protocol was developed to analyse the genome sequence stability of the modified live PRRSV vaccine strain, Porcilis MLV. The backbone of the ARTIC-style protocol was formed by 34 individual primer pairs, which were divided into two primer pools. Primer pairs were designed to amplify 532 to 588 bp fragments of the corresponding genomic region. The amplicons are suitable for sequencing on Illumina DNA sequencers with available 600-cycle sequencing kits. The concentration of primer pairs in the pools was optimized to obtain a balanced sequencing depth along the genome. Deep sequencing data of three vaccine batches were also analysed. All three vaccine batches were very similar to each other, although they also showed single nucleotide variations (SNVs) affecting less than 1 % of the genome. In the three vaccine strains, 113 to 122 SNV sites were identified; at these sites, the minority variants represented a frequency range of 1 to 48.7 percent. Additionally, the strains within the batches contained well-known length polymorphisms; the genomes of these minority deletion mutants were 135 to 222 bp shorter than the variant with the complete genome. Our results show the usefulness of ARTIC-style protocols in the evaluation of the genomic stability of PRRS MLV strains. LA - English DB - MTMT ER -