TY - JOUR AU - Mwalugha, Heltan M. AU - Molnár, Krisztina AU - Rácz, Csaba AU - Kovács, Szilvia AU - Adácsi, Cintia AU - Dövényi-Nagy, Tamás AU - Bakó, Károly István AU - Pócsi, István AU - Dobos, Attila Csaba AU - Pusztahelyi, Tünde TI - Irrigation, Nitrogen Supplementation, and Climatic Conditions Affect Resistance to Aspergillus flavus Stress in Maize JF - AGRICULTURE-BASEL J2 - AGRICULTURE-BASEL VL - 15 PY - 2025 IS - 7 PG - 34 SN - 2077-0472 DO - 10.3390/agriculture15070767 UR - https://m2.mtmt.hu/api/publication/36075800 ID - 36075800 AB - Maize production is increasingly challenged by climate change, which affects plant physiology, fungal colonization, and mycotoxin contamination. Aspergillus flavus, a saprophytic fungus, thrives in warm, dry conditions, leading to aflatoxin B1 (AFB1) accumulation, and posing significant food safety risks. Macro- and micro-climatic factors, including temperature, humidity, and precipitation, influence kernel development, leaf wetness duration, and mycotoxin biosynthesis. Nitrogen availability and irrigation play crucial roles in modulating plant responses to these stressors, affecting chlorophyll content, yield parameters, and fungal interactions. To investigate these interactions, a Completely Randomized Design (CRD) was employed from 2020 to 2022 to assess physiological changes in SY Orpheus maize hybrid under varying climatic conditions. Rising temperatures and declining relative humidity (RH) significantly reduced kernel number per ear length from 25.60 ± 0.34 in 2020 to 17.89 ± 0.39 in 2022 (p < 0.05), impacting yield. The AFB1 levels peaked in 2021 (156.88 ± 59.02 µg/kg), coinciding with lower humidity and increased fungal stress. Water availability improved kernel numbers and reduced AFB1 accumulation (p < 0.05) but did not significantly affect the total fungal load (p > 0.05). Nitrogen supplementation enhanced plant vigor, suppressed AFB1 biosynthesis, and influenced spectral indices. Potential confounding factors such as soil variability and microbial interactions may require further investigations. LA - English DB - MTMT ER - TY - CONF AU - Mwalugha, Heltan M. AU - Molnár, Krisztina AU - Rácz, Csaba AU - Kovács, Szilvia AU - Adácsi, Cintia AU - Dövényi-Nagy, Tamás AU - Bakó, Károly István AU - Pócsi, István AU - Dobos, Attila Csaba AU - Pusztahelyi, Tünde TI - Physiological and biochemical effects of toxigenic Aspergillus flavus on maize (Zea mays) T2 - MDPI The 3rd International Online Conference on Toxins : Program and absztract book PY - 2025 SP - 90 UR - https://m2.mtmt.hu/api/publication/36406106 ID - 36406106 AB - Aspergillus flavus is a major fungal pathogen in maize, significantly impacting kernel quality and food safety through mycotoxin contamination. This study examined the physiological and biochemical effects of A. flavus inoculation on maize, assessing kernel production, fungal proliferation, mycotoxin accumulation, and key biochemical parameters. A controlled field experiment was conducted, comparing inoculated (IN) and control (CT) maize throughout three years. Results showed that inoculated maize exhibited a significant reduction in kernel number per ear length (20.14 ? 0.43 vs. 21.25 ? 0.33; p < 0.05), indicating compromised reproductive success. Mold count was significantly higher in IN maize (6.32 ? 0.13 log CFU/g) compared to CT (5.55 ? 0.17 log CFU/g; p < 0.01), confirming enhanced fungal colonization. Aflatoxin B1 (AFB1) concentration increased drastically in IN maize (139.46 ? 38.64 ?g/kg) compared to CT (0.11 ? 0.07 ?g/kg; p < 0.01), posing serious food safety concerns. However, no significant differences were observed in fumonisin B1, deoxynivalenol, zearalenone, or starch, protein, and total polyphenols between treatments. Our results suggested that short-term fungal infection primarily affected the kernel formation, and not its biochemical composition or Fusarium mycotoxin accumulation. These findings emphasize the critical role of A. flavus in maize contamination and highlight the necessity of mitigation strategies to reduce fungal colonization and aflatoxin accumulation, ensuring maize safety and quality. LA - English DB - MTMT ER - TY - JOUR AU - Heltan, M. Mwaluqha AU - Molnár, Krisztina AU - Rácz, Csaba AU - Kovács, Szilvia AU - Adácsi, Cintia AU - Dövényi-Nagy, Tamás AU - Bakó, Károly István AU - Pócsi, István AU - Dobos, Attila Csaba AU - Pusztahelyi, Tünde TI - Physiological and biochemical responses of maize to Aspergillus flavus under irrigation and nitrogen regimes JF - POLNOHOSPODARSTVO-AGRICULTURE J2 - POLNOHOSPODARSTVO-AGRICULTURE PY - 2025 SN - 0551-3677 UR - https://m2.mtmt.hu/api/publication/36426573 ID - 36426573 LA - English DB - MTMT ER - TY - JOUR AU - Gálné Remenyik, Judit AU - Csige, László AU - Dávid, Péter AU - Fauszt, Péter AU - Szilágyi-Rácz, Anna Anita AU - Szőllősi, Erzsébet AU - Bacsó, Zsófia Réka AU - Szepsy Jnr, István AU - Molnár, Krisztina AU - Rácz, Csaba AU - Fidler, Gábor AU - Kállai, Zoltán AU - Stündl, László AU - Dobos, Attila Csaba AU - Paholcsek, Melinda TI - Exploring the interplay between the core microbiota, physicochemical factors, agrobiochemical cycles in the soil of the historic tokaj mád wine region JF - PLOS ONE J2 - PLOS ONE VL - 19 PY - 2024 IS - 4 PG - 24 SN - 1932-6203 DO - 10.1371/journal.pone.0300563 UR - https://m2.mtmt.hu/api/publication/34832088 ID - 34832088 N1 - Center for Complex Systems and Microbiome Innovations, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary Research Laboratory and Wine Academy of Mad, University of Debrecen, Mád, Hungary Centre for Precision Farming R&D Services, Faculty of Agriculture, Food Science and Environmental Management, University of Debrecen, Debrecen, Hungary Department of Genetics and Applied Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary Institute of Food Technology, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary Export Date: 26 September 2024 CODEN: POLNC Correspondence Address: Paholcsek, M.; Center for Complex Systems and Microbiome Innovations, Hungary; email: paholcsek.melinda@agr.unideb.hu AB - A Hungarian survey of Tokaj-Mád vineyards was conducted. Shotgun metabarcoding was applied to decipher the microbial-terroir. The results of 60 soil samples showed that there were three dominant fungal phyla, Ascomycota 66.36% ± 15.26%, Basidiomycota 18.78% ± 14.90%, Mucoromycota 11.89% ± 8.99%, representing 97% of operational taxonomic units (OTUs). Mutual interactions between microbiota diversity and soil physicochemical parameters were revealed. Principal component analysis showed descriptive clustering patterns of microbial taxonomy and resistance gene profiles in the case of the four historic vineyards (Szent Tamás, Király, Betsek, Nyúlászó). Linear discriminant analysis effect size was performed, revealing pronounced shifts in community taxonomy based on soil physicochemical properties. Twelve clades exhibited the most significant shifts (LDA > 4.0), including the phyla Verrucomicrobia , Bacteroidetes , Chloroflexi , and Rokubacteria , the classes Acidobacteria , Deltaproteobacteria , Gemmatimonadetes , and Betaproteobacteria , the order Sphingomonadales , Hypomicrobiales , as well as the family Sphingomonadaceae and the genus Sphingomonas . Three out of the four historic vineyards exhibited the highest occurrences of the bacterial genus Bradyrhizobium , known for its positive influence on plant development and physiology through the secretion of steroid phytohormones. During ripening, the taxonomical composition of the soil fungal microbiota clustered into distinct groups depending on altitude, differences that were not reflected in bacteriomes. Network analyses were performed to unravel changes in fungal interactiomes when comparing postveraison and preharvest samples. In addition to the arbuscular mycorrhiza Glomeraceae , the families Mycosphaerellacae and Rhyzopodaceae and the class Agaricomycetes were found to have important roles in maintaining soil microbial community resilience. Functional metagenomics showed that the soil Na content stimulated several of the microbiota-related agrobiogeochemical cycles, such as nitrogen and sulphur metabolism; steroid, bisphenol, toluene, dioxin and atrazine degradation and the synthesis of folate. LA - English DB - MTMT ER - TY - JOUR AU - Molnár, Krisztina AU - Rácz, Csaba AU - Dövényi-Nagy, Tamás AU - Bakó, Károly István AU - Pusztahelyi, Tünde AU - Kovács, Szilvia AU - Adácsi, Cintia AU - Pócsi, István AU - Dobos, Attila Csaba TI - The Effect of Environmental Factors on Mould Counts and AFB1 Toxin Production by Aspergillus flavus in Maize JF - TOXINS J2 - TOXINS VL - 15 PY - 2023 IS - 3 PG - 18 SN - 2072-6651 DO - 10.3390/toxins15030227 UR - https://m2.mtmt.hu/api/publication/33705114 ID - 33705114 N1 - Centre for Precision Farming R&D Services, FAFSEM, University of Debrecen, Debrecen, H4032, Hungary Central Laboratory of Agricultural and Food Products, FAFSEM, University of Debrecen, Debrecen, H4032, Hungary Department of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, Debrecen, H4032, Hungary Export Date: 09 January 2024; Cited By: 4; Correspondence Address: K. Molnár; Centre for Precision Farming R&D Services, FAFSEM, University of Debrecen, Debrecen, H4032, Hungary; email: molnark@agr.unideb.hu AB - The toxins produced by Aspergillus flavus can significantly inhibit the use of maize. As a result of climate change, toxin production is a problem not only in tropical and subtropical areas but in an increasing number of European countries, including Hungary. The effect of meteorological factors and irrigation on mould colonization and aflatoxin B1 (AFB1) mycotoxin production by A. flavus were investigated in natural conditions, as well as the inoculation with a toxigenic isolate in a complex field experiment for three years. As a result of irrigation, the occurrence of fungi increased, and toxin production decreased. The mould count of fungi and toxin accumulation showed differences during the examined growing seasons. The highest AFB1 content was found in 2021. The main environmental factors in predicting mould count were temperature (Tavg, Tmax ≥ 30 °C, Tmax ≥ 32 °C, Tmax ≥ 35 °C) and atmospheric drought (RHmin ≤ 40%). Toxin production was determined by extremely high daily maximum temperatures (Tmax ≥ 35 °C). At natural contamination, the effect of Tmax ≥ 35 °C on AFB1 was maximal (r = 0.560–0.569) in the R4 stage. In the case of artificial inoculation, correlations with environmental factors were stronger (r = 0.665–0.834) during the R2–R6 stages. LA - English DB - MTMT ER - TY - JOUR AU - Pusztahelyi, Tünde AU - Adácsi, Cintia AU - Kovács, Szilvia AU - Dobos, Attila Csaba AU - Pfliegler, Valter Péter AU - Madhavan Nampoothiri, K. AU - Pócsi, István TI - Mycotoxins in food chain-climate effect and elimination studies JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 70 PY - 2023 IS - Supplement 1 SP - 77 EP - 78 PG - 2 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/34141829 ID - 34141829 LA - English DB - MTMT ER - TY - CONF AU - Pócsi, István AU - Murvai, Katalin AU - Horváth, Enikő AU - Bodnár , Veronika AU - Mondok, Ágnes AU - Adácsi, Cintia AU - Molnár, Krisztina AU - Dobos, Attila Csaba AU - Leiter, Éva Juliánna AU - Pfliegler, Valter Péter AU - Pusztahelyi, Tünde TI - Recent Results in Aflatoxin Research at the University of Debrecen T2 - International Conference on New Horizons in Biotechnology ABSTRACT BOOK PY - 2023 SP - 110 UR - https://m2.mtmt.hu/api/publication/34447668 ID - 34447668 LA - English DB - MTMT ER - TY - JOUR AU - Farkas, Zsuzsa AU - Országh, Erika AU - Engelhardt, Tekla AU - Csorba, Szilveszter AU - Kerekes, Kata AU - Zentai, Andrea AU - Süth, Miklós AU - Nagy, A AU - Miklós, Gabriella AU - Molnár, Krisztina AU - Rácz, Csaba AU - Dövényi-Nagy, Tamás AU - Ambrus, Árpád AU - Győri, Zoltán AU - Dobos, Attila Csaba AU - Pusztahelyi, Tünde AU - Pócsi, István AU - Józwiak, Ákos Bernard TI - A Systematic Review of the Efficacy of Interventions to Control Aflatoxins in the Dairy Production Chain—Feed Production and Animal Feeding Interventions JF - TOXINS J2 - TOXINS VL - 14 PY - 2022 IS - 2 PG - 34 SN - 2072-6651 DO - 10.3390/toxins14020115 UR - https://m2.mtmt.hu/api/publication/32644330 ID - 32644330 N1 - Digital Food Institute, University of Veterinary Medicine Budapest, Budapest, 1078, Hungary System Management and Supervision Directorate, National Food Chain Safety Office, Budapest, 1024, Hungary Food Chain Safety Laboratory Directorate, National Food Chain Safety Office, Budapest, 1095, Hungary Székesfehérvár Regional Food Chain Laboratory, National Food Chain Safety Office, Székesfehérvár, 8000, Hungary Centre for Precision Farming R&D Services, Faculty of Agriculture, Food Science and Environmental Management, University of Debrecen, Debrecen, 4032, Hungary Doctoral School of Nutrition and Food Sciences, University of Debrecen, Debrecen, 4032, Hungary Institute of Nutrition, Faculty of Agricultural, Food and Environmental Management of University of Debrecen, Debrecen, 4032, Hungary Central Laboratory of Agricultural and Food Products, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Debrecen, 4032, Hungary Department of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, Debrecen, 4032, Hungary Export Date: 25 July 2022 Correspondence Address: Farkas, Z.; Digital Food Institute, Hungary; email: farkas.zsuzsa@univet.hu LA - English DB - MTMT ER - TY - CHAP AU - Pusztahelyi, Tünde AU - Kovács, Szilvia AU - Adácsi, Cintia AU - Miklós, Ida AU - Dobos, Attila Csaba AU - Győri, Zoltán AU - Madhavan Nampoothiri, K. AU - Pócsi, István TI - Biological control and elimination of mycotoxins in food and feed T2 - Biotechnology at the University of Debrecen: 2022 International Symposium : Abstract Book PB - University of Debrecen C1 - Debrecen SN - 9789634904670 PY - 2022 SP - 17 UR - https://m2.mtmt.hu/api/publication/33285495 ID - 33285495 LA - English DB - MTMT ER -