TY - JOUR AU - Bodnár , Veronika AU - Antal, Károly AU - de Vries, Ronald P. AU - Pócsi, István AU - Emri, Tamás TI - Aspergillus nidulans gfdB, Encoding the Hyperosmotic Stress Protein Glycerol-3-phosphate Dehydrogenase, Disrupts Osmoadaptation in Aspergillus wentii JF - JOURNAL OF FUNGI J2 - J FUNGI VL - 10 PY - 2024 IS - 4 PG - 23 SN - 2309-608X DO - 10.3390/jof10040291 UR - https://m2.mtmt.hu/api/publication/34802432 ID - 34802432 AB - The genome of the osmophilic Aspergillus wentii, unlike that of the osmotolerant Aspergillus nidulans, contains only the gfdA, but not the gfdB, glycerol 3-phosphate dehydrogenase gene. Here, we studied transcriptomic changes of A. nidulans (reference strain and ΔgfdB gene deletion mutant) and A. wentii (reference strain and An-gfdB expressing mutant) elicited by high osmolarity. A. nidulans showed a canonic hyperosmotic stress response characterized by the upregulation of the trehalose and glycerol metabolism genes (including gfdB), as well as the genes of the high-osmolarity glycerol (HOG) map kinase pathway. The deletion of gfdB caused only negligible alterations in the transcriptome, suggesting that the glycerol metabolism was flexible enough to compensate for the missing GfdB activity in this species. A. wentii responded differently to increased osmolarity than did A. nidulans, e.g., the bulk upregulation of the glycerol and trehalose metabolism genes, along with the HOG pathway genes, was not detected. The expression of An-gfdB in A. wentii did not abolish osmophily, but it reduced growth and caused much bigger alterations in the transcriptome than did the missing gfdB gene in A. nidulans. Flexible glycerol metabolism and hence, two differently regulated gfd genes, may be more beneficial for osmotolerant (living under changing osmolarity) than for osmophilic (living under constantly high osmolarity) species. LA - English DB - MTMT ER - TY - CONF AU - Imre, Alexandra AU - Kovács, Renátó AU - Ibrahim, Al’ Abri AU - Nathan, Crook AU - Pfliegler, Valter Péter TI - Specific high effect mutations in clinical and experimentally evolved Saccharomyces ‘boulardii’ isolates show that genes involved in chemical response might have a role during the adaptation to the human host T2 - The Allied Genetics Conference 2024 Abstract Book PY - 2024 UR - https://m2.mtmt.hu/api/publication/34753304 ID - 34753304 LA - English DB - MTMT ER - TY - JOUR AU - Emri, Tamás AU - Antal, Károly AU - Varga, Kinga AU - Gila, Csaba Barnabás AU - Pócsi, István TI - The Oxidative Stress Response Highly Depends on Glucose and Iron Availability in Aspergillus fumigatus JF - JOURNAL OF FUNGI J2 - J FUNGI VL - 10 PY - 2024 IS - 3 PG - 18 SN - 2309-608X DO - 10.3390/jof10030221 UR - https://m2.mtmt.hu/api/publication/34750126 ID - 34750126 AB - Pathogens have to cope with oxidative, iron- and carbon(glucose)-limitation stresses in the human body. To understand how combined iron–carbon limitation alters oxidative stress responses, Aspergillus fumigatus was cultured in glucose–peptone or peptone containing media supplemented or not with deferiprone as an iron chelator. Changes in the transcriptome in these cultures were recorded after H2O2 treatment. Responses to oxidative stress were highly dependent on the availability of glucose and iron. Out of the 16 stress responsive antioxidative enzyme genes, only the cat2 catalase–peroxidase gene was upregulated in more than two culturing conditions. The transcriptional responses observed in iron metabolism also varied substantially in these cultures. Only extracellular siderophore production appeared important regardless of culturing conditions in oxidative stress protection, while the enhanced synthesis of Fe-S cluster proteins seemed to be crucial for oxidative stress treated iron-limited and fast growing (glucose rich) cultures. Although pathogens and host cells live together in the same place, their culturing conditions (e.g., iron availability or occurrence of oxidative stress) can be different. Therefore, inhibition of a universally important biochemical process, like Fe-S cluster assembly, may selectively inhibit the pathogen growth in vivo and represent a potential target for antifungal therapy. LA - English DB - MTMT ER - TY - JOUR AU - Laczkó, Levente AU - Jordán, Sándor AU - Póliska, Szilárd AU - Rácz, Hanna Viktória AU - Nagy, Nikoletta Andrea AU - Molnár, V. Attila AU - Sramkó, Gábor TI - The draft genome of Spiraea crenata L. (Rosaceae) – the first complete genome in tribe Spiraeeae JF - SCIENTIFIC DATA J2 - SCI DATA VL - 11 PY - 2024 IS - 1 PG - 11 SN - 2052-4463 DO - 10.1038/s41597-024-03046-0 UR - https://m2.mtmt.hu/api/publication/34635507 ID - 34635507 AB - Spiraea crenata L. is a deciduous shrub distributed across the Eurasian steppe zone. The species is of cultural and horticultural importance and occurs in scattered populations throughout its westernmost range. Currently, there is no genomic information on the tribe of Spiraeeae. Therefore we sequenced and assembled the whole genome of S. crenata using second- and third-generation sequencing and a hybrid assembly approach to expand genomic resources for conservation and support research on this horticulturally important lineage. In addition to the organellar genomes (the plastome and the mitochondrion), we present the first draft genome of the species with an estimated size of 220 Mbp, an N50 value of 7.7 Mbp, and a BUSCO score of 96.0%. Being the first complete genome in tribe Spiraeeae, this may not only be the first step in the genomic study of a rare plant but also a contribution to genomic resources supporting the study of biodiversity and evolutionary history of Rosaceae. LA - English DB - MTMT ER - TY - JOUR AU - Balogh, Zoltán AU - Len, Adél AU - Baksa, Viktória AU - Krajnc, Andraž AU - Herman, Petra AU - Szemán-Nagy, Gábor AU - Czigány, Zsolt AU - Fábián, István AU - Kalmár, József AU - Dudás, Zoltán Imre TI - Nanoscale structural characteristics and in vitro bioactivity of borosilicate – polyvinyl alcohol (PVA) hybrid aerogels for bone regeneration JF - ACS APPLIED NANO MATERIALS J2 - ACS APPL NANO MATER VL - 7 PY - 2024 IS - 4 SP - 4092 EP - 4102 PG - 11 SN - 2574-0970 DO - 10.1021/acsanm.3c05668 UR - https://m2.mtmt.hu/api/publication/34538335 ID - 34538335 LA - English DB - MTMT ER - TY - JOUR AU - Jakab, Ágnes AU - Csillag, Kinga Karola AU - Antal, Károly AU - Boczonádi, Imre AU - Kovács, Renátó AU - Pócsi, István AU - Emri, Tamás TI - Total transcriptome response for tyrosol exposure in Aspergillus nidulans JF - FUNGAL BIOLOGY J2 - FUNGAL BIOL-UK VL - 128 PY - 2024 IS - 2 SP - 1664 EP - 1674 PG - 11 SN - 1878-6146 DO - 10.1016/j.funbio.2024.01.003 UR - https://m2.mtmt.hu/api/publication/34524101 ID - 34524101 N1 - Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary Department of Molecular Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, Debrecen, 4032, Hungary Department of Zoology, Faculty of Sciences, Eszterházy Károly Catholic University, Eger, 3300, Hungary HUN-REN–UD Fungal Stress Biology Research Group, Debrecen, 4032, Hungary Export Date: 18 February 2024 Correspondence Address: Jakab, Á.; Department of Medical Microbiology, Nagyerdei krt. 98., Hungary; email: jakab.agnes@med.unideb.hu Funding details: Magyar Tudományos Akadémia, MTA Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI Funding details: National Research, Development and Innovation Office, FK138462, K131767, TKP2021-EGA-20 Funding text 1: Research was financed by the National Research, Development, and Innovation Office (Hungary) projects K131767 , and FK138462 . Project no. TKP2021-EGA-20 (Biotechnology) has been implemented with the support provided from the National Research, Development and Innovation Fund of Hungary , financed under the TKP2021-EGA funding scheme. R. K. was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences . LA - English DB - MTMT ER - TY - JOUR AU - Vipin Krishnan, S. AU - Madhavan Nampoothiri, K. AU - Suresh, Anandhu AU - Thuy Linh, Nguyen AU - Balakumaran, P. A. AU - Pócsi, István AU - Pusztahelyi, Tünde TI - Fusarium biocontrol: antagonism and mycotoxin elimination by lactic acid bacteria JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 14 PY - 2024 SN - 1664-302X DO - 10.3389/fmicb.2023.1260166 UR - https://m2.mtmt.hu/api/publication/34416277 ID - 34416277 AB - Mycotoxins produced by Fusarium species are secondary metaboliteswith low molecular weight formed by filamentous fungi generally resistant to different environmental factors and, therefore, undergo slow degradation. Contamination by Fusarium mycotoxins in cereals and millets is the foremost quality challenge the food and feed industry faces across the globe. Several types of chemical preservatives are employed in the mitigation process of these mycotoxins, and they help in long-term storage; however, chemical preservatives can be used only to some extent, so the complete elimination of toxins from foods is still a herculean task. The growing demand for green-labeled food drives to evade the use of chemicals in the production processes is getting much demand. Thus, the biocontrol of food toxins is important in the developing food sector. Fusarium mycotoxins are world-spread contaminants naturally occurring in commodities, food, and feed. The major mycotoxins Fusarium species produce are deoxynivalenol, fumonisins, zearalenone, and T2/HT2 toxins. Lactic acid bacteria (LAB), generally regarded as safe (GRAS), is a well-explored bacterial community in food preparations and preservation for ages. Recent research suggests that LAB are the best choice for extenuating Fusarium mycotoxins. Apart from Fusarium mycotoxins, this review focuses on the latest studies on the mechanisms of how LAB effectively detoxify and remove these mycotoxins through their various bioactive molecules and background information of these molecules. LA - English DB - MTMT ER - TY - JOUR AU - Pákozdi, Klaudia Gréta AU - Szabó, Zsuzsa AU - Katalin, Pappné-Murvai AU - Géza, Hegedűs AU - Emri, Tamás AU - Pócsi, István AU - Virág, Eszter Andrea TI - Transcriptomic adaptation to superoxide stress in fvatfa and fvmnsod fusarium verticilloides strains JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 70 PY - 2023 IS - Supplement 1 SP - 75 EP - 76 PG - 2 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/34457284 ID - 34457284 LA - English DB - MTMT ER - TY - JOUR AU - Bodnár , Veronika AU - Pákozdi, Klaudia Gréta AU - Király, Anita AU - Póliska, Szilárd AU - Antal, Károly AU - Leiter, Éva Juliánna AU - Pócsi, István AU - Emri, Tamás TI - Osmotic stress elicited gene expression changes in aspergillus wentii wildtype and 'c gfdb and aspergillus nidulans wild-type and gfdb mutant strains JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 70 PY - 2023 IS - Supplement 1 SP - 58 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/34457279 ID - 34457279 LA - English DB - MTMT ER - TY - CONF AU - Emri, Tamás AU - Leiter, Éva Juliánna AU - Pócsi, István TI - Elements and regulalation of Cd2+ stress response in the Aspergilli T2 - 21st Jena Remediation Symposium: BioGeo interfaces under stress, Proceedings PB - Friedrich-Schiller-Universität Jena C1 - Jena PY - 2023 SP - 19 UR - https://m2.mtmt.hu/api/publication/34457257 ID - 34457257 LA - English DB - MTMT ER -