TY - JOUR AU - Sebők, Csilla AU - Márton, Rege Anna AU - Mackei, Máté AU - Neogrády, Zsuzsa AU - Mátis, Gábor TI - Az antimikrobiális peptidek mint a fertőző betegségek elleni küzdelem új eszközei JF - MAGYAR ÁLLATORVOSOK LAPJA J2 - MAGY ALLATORVOSOK VL - 146 PY - 2024 IS - 3 SP - 181 EP - 191 PG - 11 SN - 0025-004X DO - 10.56385/magyallorv.2024.03.181-191 UR - https://m2.mtmt.hu/api/publication/34758378 ID - 34758378 AB - Az antimikrobiális peptidek (továbbiakban: AMP-k), vagy a szakirodalomban egyre ismertebbé váló nevükön gazdavédő peptidek (host defense peptide, HDP) a veleszületett immunitás részeként csaknem minden szervezetben megtalálhatóak, és közvetlen antimikrobiális hatásukon kívül jelentős immunmoduláns, valamint daganatellenes és sebgyógyulást serkentő képességekkel rendelkeznek. A szakirodalmi összefoglalóban a szerzők bemutatják az AMP-k általános tulajdonságait, ill. felfedezésük történetét, majd részletesen ismertetik közvetlen és közvetett antimikrobiális hatásaikat. LA - Hungarian DB - MTMT ER - TY - JOUR AU - Márton, Rege Anna AU - Sebők, Csilla AU - Mackei, Máté AU - Tráj, Patrik AU - Vörösházi, Júlia AU - Kemény, Ágnes AU - Neogrády, Zsuzsa AU - Mátis, Gábor TI - Cecropin A: investigation of a host defense peptide with multifaceted immunomodulatory activity in a chicken hepatic cell culture JF - FRONTIERS IN VETERINARY SCIENCE J2 - FRONT VET SCI VL - 11 PY - 2024 PG - 10 SN - 2297-1769 DO - 10.3389/fvets.2024.1337677 UR - https://m2.mtmt.hu/api/publication/34718275 ID - 34718275 N1 - project no. RRF-2.3.1-21-2022-00001 has been implemented with the support provided by the Recovery and Resilience Facility (RRF), financed under the National Recovery Fund budget estimate, RRF-2.3.1-21 funding scheme. AB - IntroductionHost defense peptides (HDPs) are increasingly referred to as promising candidates for the reduction of the use of conventional antibiotics, thereby combating antibiotic resistance. As HDPs have been described to exert various immunomodulatory effects, cecropin A (CecA) appears to be a potent agent to influence the host inflammatory response.MethodsIn the present study, a chicken primary hepatocyte–non-parenchymal cell co-culture was used to investigate the putative immunomodulatory effects of CecA alone and in inflammatory conditions evoked by polyinosinic-polycytidylic acid (Poly I:C). To examine the viability of the cells, the extracellular lactate dehydrogenase (LDH) activity was determined by colorimetric assay. Inflammatory markers interleukin (IL)-8 and transforming growth factor-ß1 (TGF-ß1) were investigated using the ELISA method, whereas concentrations of IL-6, IL-10, and interferon-γ (IFN-γ) were assayed by Luminex xMAP technology. Extracellular H2O2 and malondialdehyde levels were measured by fluorometric and colorimetric methods, respectively.ResultsResults of the lower concentrations suggested the safe application of CecA; however, it might contribute to hepatic cell membrane damage at its higher concentrations. We also found that the peptide alleviated the inflammatory response, reflected by the decreased production of the pro-inflammatory IL-6, IL-8, and IFN-γ. In addition, CecA diminished the levels of anti-inflammatory IL-10 and TGF-ß1. The oxidative markers measured remained unchanged in most cases of CecA exposure.DiscussionCecA displayed a multifaceted immunomodulatory but not purely anti-inflammatory activity on the hepatic cells, and might be suggested to maintain the hepatic inflammatory homeostasis in Poly I:C-triggered immune response. To conclude, our study suggests that CecA might be a promising molecule for the development of new immunomodulatory antibiotic-substitutive agents in poultry medicine; however, there is still a lot to clarify regarding its cellular effects. LA - English DB - MTMT ER - TY - JOUR AU - Vörösházi, Júlia AU - Neogrády, Zsuzsa AU - Mátis, Gábor AU - Mackei, Máté TI - Pathological consequences, metabolism and toxic effects of trichothecene T-2 toxin in poultry JF - POULTRY SCIENCE J2 - POULTRY SCI VL - 103 PY - 2024 IS - 3 SP - 103471 SN - 0032-5791 DO - 10.1016/j.psj.2024.103471 UR - https://m2.mtmt.hu/api/publication/34718274 ID - 34718274 N1 - Project no. RRF-2.3.1-21-2022-00001 has been implemented with the support provided by the Recovery and Resilience Facility (RRF), financed under the National Recovery Fund budget estimate, RRF-2.3.1-21 funding scheme. AB - Contamination of feed with mycotoxins has become a severe issue worldwide. Among the most prevalent trichothecene mycotoxins, T-2 toxin is of particular importance for livestock production, including poultry posing a significant threat to animal health and productivity. This review article aims to comprehensively analyze the pathological consequences, metabolism, and toxic effects of T-2 toxin in poultry. Trichothecene mycotoxins, primarily produced by Fusarium species, are notorious for their potent toxicity. T-2 toxin exhibits a broad spectrum of negative effects on poultry species, leading to substantial economic losses as well as concerns about animal welfare and food safety in modern agriculture. T-2 toxin exposure easily results in negative pathological consequences in the gastrointestinal tract, as well as in parenchymal tissues like the liver (as the key organ for its metabolism), kidneys, or reproductive organs. In addition, it also intensely damages immune system-related tissues such as the spleen, the bursa of Fabricius, or the thymus causing immunosuppression and increasing the susceptibility of the animals to infectious diseases, as well as making immunization programs less effective. The toxin also damages cellular processes on the transcriptional and translational levels and induces apoptosis through the activation of numerous cellular signaling cascades. Furthermore, according to recent studies, besides the direct effects on the abovementioned processes, T-2 toxin induces the production of reactive molecules and free radicals resulting in oxidative distress and concomitantly occurring cellular damage. In conclusion, this review article provides a complex and detailed overview of the metabolism, pathological consequences, mechanism of action as well as the immunomodulatory and oxidative stress-related effects of T-2 toxin. Understanding these effects in poultry is crucial for developing strategies to mitigate the impact of the T-2 toxin on avian health and food safety in the future. LA - English DB - MTMT ER - TY - JOUR AU - Berlinger, Balázs AU - Fehérvári, Péter AU - Kővágó, Csaba AU - Lányi, Katalin AU - Mátis, Gábor AU - Mackei, Máté AU - Könyves, László TI - There Is Still a Need for a Comprehensive Investigation of the Health Consequences of Exposure to Urban Air with Special Regard to Particulate Matter (PM) and Cardiovascular Effects JF - ATMOSPHERE J2 - ATMOSPHERE-BASEL VL - 15 PY - 2024 IS - 3 SP - 296 SN - 2073-4433 DO - 10.3390/atmos15030296 UR - https://m2.mtmt.hu/api/publication/34714134 ID - 34714134 AB - Air pollution is a foremost public health problem worldwide. The pulmonary effects of air pollution have been well established for decades, and substantial epidemiological evidence is consistently showing that both acute and chronic exposure to air pollution is linked to cardiovascular morbidity and mortality. The underlying cause for this link is, however, still unknown, and many questions remain open. Most of the epidemiological studies focusing on health consequences of exposure to urban air used data from air monitoring stations or—when applying personal sampling or monitoring—measured a limited number of components to assess the exposure. On the other hand, studies conducting a decent exposure characterization and measuring a relatively large number of components with personal sampling had little or no focus on the effects and investigated only a few biomarkers. The number of research studies on this topic is huge, but at the same time, it seems as if there was no need for a comprehensive examination of the effects of urban air pollution on health. Researchers and research supporting organizations, in their fascination with the search for “novelty” and “beyond state-of-the-art”, do not seem to be aware of that they will never be able to assemble the whole picture from the puzzle pieces of research activities focusing only on certain aspects. Without a comprehensive investigation, we might never be able to answer such questions as (i) which of the urban air pollutants are forerunners in causing health effects, especially cardiovascular effects? (ii) Which biomarkers have the best predictor performance in early effects? (iii) Are there biomarkers or combinations of biomarkers that can help determine the degree of individual susceptibility and sensitivity and the dependence of these by different factors (e.g., age, gender, and physical activity)? The authors of this article aim to go through the already investigated—at the same time, essential—elements of the topic and propose a more comprehensive study, which, of course, can be expanded, debated, and improved. LA - English DB - MTMT ER - TY - JOUR AU - Vörösházi, Júlia AU - Mackei, Máté AU - Sebők, Csilla AU - Tráj, Patrik AU - Márton, Rege Anna AU - Horváth, Dávid Géza AU - Huber, Korinna AU - Neogrády, Zsuzsa AU - Mátis, Gábor TI - Investigation of the effects of T-2 toxin in chicken-derived three-dimensional hepatic cell cultures JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 16 SN - 2045-2322 DO - 10.1038/s41598-024-51689-1 UR - https://m2.mtmt.hu/api/publication/34572947 ID - 34572947 N1 - Funding Agency and Grant Number: University of Veterinary Medicine; Hungarian National Research, Development and Innovation Office [OTKA FK 134940]; University of Veterinary Medicine Budapest [SRF-001]; Recovery and Resilience Facility (RRF) - National Recovery Fund budget estimate [RRF-2.3.1-21-2022-00001, RRF-2.3.1-21]; German Research Foundation [DFG-INST 36/152-1 FUGG] Funding text: Open access funding provided by University of Veterinary Medicine. The work was supported by the Hungarian National Research, Development and Innovation Office (Grant number OTKA FK 134940), by the strategic research fund of the University of Veterinary Medicine Budapest (Grant no. SRF-001.). Project no. RRF-2.3.1-21-2022-00001 has been implemented with the support provided by the Recovery and Resilience Facility (RRF), financed under the National Recovery Fund budget estimate, RRF-2.3.1-21 funding scheme. The QTRAP 5500 mass spectrometer was co-funded by the German Research Foundation (DFG-INST 36/152-1 FUGG) The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. AB - Despite being one of the most common contaminants of poultry feed, the molecular effects of T-2 toxin on the liver of the exposed animals are still not fully elucidated. To gain more accurate understanding, the effects of T-2 toxin were investigated in the present study in chicken-derived three-dimensional (3D) primary hepatic cell cultures. 3D spheroids were treated with three concentrations (100, 500, 1000 nM) of T-2 toxin for 24 h. Cellular metabolic activity declined in all treated groups as reflected by the Cell Counting Kit-8 assay, while extracellular lactate dehydrogenase activity was increased after 500 nM T-2 toxin exposure. The levels of oxidative stress markers malondialdehyde and protein carbonyl were reduced by the toxin, suggesting effective antioxidant compensatory mechanisms of the liver. Concerning the pro-inflammatory cytokines, IL-6 concentration was decreased, while IL-8 concentration was increased by 100 nM T-2 toxin exposure, indicating the multifaceted immunomodulatory action of the toxin. Further, the metabolic profile of hepatic spheroids was also modulated, confirming the altered lipid and amino acid metabolism of toxin-exposed liver cells. Based on these results, T-2 toxin affected cell viability, hepatocellular metabolism and inflammatory response, likely carried out its toxic effects by affecting the oxidative homeostasis of the cells. LA - English DB - MTMT ER - TY - JOUR AU - Kissné Hatala, Patrícia AU - Sebők, Csilla AU - Mackei, Máté AU - Karpati, Karina AU - Gálfi, Péter AU - Neogrády, Zsuzsa AU - Mátis, Gábor TI - Molecular effects of intermittent stress on primary feline uroepithelial cell culture as an in vitro model of feline idiopathic cystitis JF - FRONTIERS IN VETERINARY SCIENCE J2 - FRONT VET SCI VL - 10 PY - 2023 PG - 10 SN - 2297-1769 DO - 10.3389/fvets.2023.1258375 UR - https://m2.mtmt.hu/api/publication/34443263 ID - 34443263 AB - Introduction: The most common cause of feline lower urinary tract disease (FLUDT) is feline idiopathic cystitis (FIC), which is a complex multifactorial disease with symptoms including stranguria, dysuria, hematuria, and pain during urination. The development of these symptoms is often triggered by stress, and in case of chronic stress, these symptoms will many times return. One of the most important stress hormones in the pathogenesis of FIC is norepinephrine (NE), as persistently elevated level of this hormone can be measured in the blood of cats with FIC. However, it is not well understood if recurrently elevated level of NE has any direct effect on urinary bladder, therefore the aim of this study was to investigate the molecular effects of intermittent NE exposure on feline primary uroepithelial cell culture.Methods: Primary uroepithelial cells were gained from the mucosa of the bladder of a euthanized cat, and were cultured for 6 days, then they were exposed to 10, 100, and 1,000 mu M NE treatment for 3 x 1 h, including a 1 h long regeneration period between exposures.Results: NE was able to trigger pro-inflammatory response and oxidative stress in the uroepithelial cells by increasing the level of stromal cell derived factor 1 (SDF-1) and H2O2 in cell culture media. In addition, NE increased the permeability of the uroepithelium, since decreased glycosaminoglycan (GAG) concentration, tight junction protein claudin-4 content, and TER values were measured after the NE treatments.Discussion: Based on these results it can be concluded that recurrent stress mimicked by 3x1 h NE treatment has a direct molecular effect on the uroepithelial cells, which leads to inflammatory response, oxidative stress and decreased barrier function of the uroepithelium. Therefore, intermittent release of NE may have an important role in the pathogenesis of FIC and the results of this study may contribute to a better understanding of the development of this illness. LA - English DB - MTMT ER - TY - JOUR AU - Sebők, Csilla AU - Tráj, Patrik AU - Mackei, Máté AU - Márton, Rege Anna AU - Vörösházi, Júlia AU - Kemény, Ágnes AU - Neogrády, Zsuzsa AU - Mátis, Gábor TI - Modulation of the immune response by the host defense peptide IDR-1002 in chicken hepatic cell culture JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 12 SN - 2045-2322 DO - 10.1038/s41598-023-41707-z UR - https://m2.mtmt.hu/api/publication/34133724 ID - 34133724 AB - IDR-1002, a synthetic host defense peptide (HDP), appears to be a potential candidate for the treatment of bacterial infections and the consequent inflammatory response due to its potent immunomodulatory activity. This is of relevance to the emerging issue of antimicrobial resistance in the farming sector. In this study, the effects of IDR-1002 were investigated on a chicken hepatocyte‒non-parenchymal cell co-culture, and the results revealed that IDR-1002 had complex effects on the regulation of the hepatic innate immunity. IDR-1002 increased the levels of both RANTES (Regulated on Activation, Normal T cell Expressed and Secreted) and Macrophage colony stimulating factor (M-CSF), suggesting the peptide plays a role in the modulation of macrophage differentiation, also reflected by the reduced concentrations of interleukin (IL)-6 and IL-10. The pro-inflammatory cytokine release triggered by the bacterial cell wall component lipoteichoic acid (LTA) was ameliorated by the concomitantly applied IDR-1002 based on the levels of IL-6, chicken chemotactic and angiogenic factor (CXCLi2) and interferon (IFN)-γ. Moreover, the production of nuclear factor erythroid 2-related factor 2 (Nrf2), an essential transcription factor in the antioxidant defense pathway, was increased after IDR-1002 exposure, while protein carbonyl (PC) levels were also elevated. These findings suggest that IDR-1002 affects the interplay of the cellular immune response and redox homeostasis, thus the peptide represents a promising tool in the treatment of bacterially induced inflammation in chickens. LA - English DB - MTMT ER - TY - JOUR AU - Mackei, Máté AU - Sebők, Csilla AU - Vörösházi, Júlia AU - Tráj, Patrik AU - Mackei, Fruzsina AU - Oláh, Barnabás AU - Fébel, Hedvig AU - Neogrády, Zsuzsa AU - Mátis, Gábor TI - Detrimental consequences of tebuconazole on redox homeostasis and fatty acid profile of honeybee brain JF - INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY J2 - INSECT BIOCHEM MOLEC VL - 159 PY - 2023 SN - 0965-1748 DO - 10.1016/j.ibmb.2023.103990 UR - https://m2.mtmt.hu/api/publication/34073645 ID - 34073645 LA - English DB - MTMT ER - TY - JOUR AU - Tráj, Patrik AU - Sebők, Csilla AU - Mackei, Máté AU - Kemény, Ágnes AU - Farkas, Orsolya AU - Kákonyi, Ákos AU - Kovács, László AU - Neogrády, Zsuzsa AU - Jerzsele, Ákos AU - Mátis, Gábor TI - Luteolin: A Phytochemical to Mitigate S. Typhimurium Flagellin-Induced Inflammation in a Chicken In Vitro Hepatic Model JF - ANIMALS J2 - ANIMALS-BASEL VL - 13 PY - 2023 IS - 8 PG - 15 SN - 2076-2615 DO - 10.3390/ani13081410 UR - https://m2.mtmt.hu/api/publication/33788876 ID - 33788876 AB - The use of natural feed supplements is an alternative tool to diminish the damage caused by certain bacteria, improving animal health and productivity. The present research aimed to investigate the proinflammatory effect of flagellin released from the bacterial flagellum of Salmonella enterica serovar Typhimurium and to attenuate the induced inflammation with luteolin as a plant-derived flavonoid on a chicken primary hepatocyte–non-parenchymal cell co-culture. Cells were cultured in a medium supplemented with 250 ng/mL flagellin and 4 or 16 µg/mL luteolin for 24 h. Cellular metabolic activity, lactate dehydrogenase (LDH) activity, interleukin-6, 8, 10 (IL-6, IL-8, IL-10), interferon-α, γ (IFN-α, IFN-γ), hydrogen peroxide (H2O2) and malondialdehyde (MDA) concentrations were determined. Flagellin significantly increased the concentration of the proinflammatory cytokine IL-8 and the ratio of IFN-γ/IL-10, while it decreased the level of IL-10, indicating that the model served adequate to study inflammation in vitro. Luteolin treatment at 4 µg/mL did not prove to be cytotoxic, as reflected by metabolic activity and extracellular LDH activity, and significantly reduced the flagellin-triggered IL-8 release of the cultured cells. Further, it had a diminishing effect on the concentration of IFN-α, H2O2 and MDA and restored the level of IL-10 and the ratio of IFN-γ/IL-10 when applied in combination with flagellin. These results suggest that luteolin at lower concentrations may protect hepatic cells from an excessive inflammatory response and act as an antioxidant to attenuate oxidative damage. LA - English DB - MTMT ER - TY - JOUR AU - Kissné Hatala, Patrícia AU - Lajos, Andrea AU - Mackei, Máté AU - Sebők, Csilla AU - Tráj, Patrik AU - Vörösházi, Júlia AU - Neogrády, Zsuzsa AU - Mátis, Gábor TI - Feline Uroepithelial Cell Culture as a Novel Model of Idiopathic Cystitis: Investigations on the Effects of Norepinephrine on Inflammatory Response, Oxidative Stress, and Barrier Function JF - VETERINARY SCIENCES J2 - VET SCI VL - 10 PY - 2023 IS - 2 SN - 2306-7381 DO - 10.3390/vetsci10020132 UR - https://m2.mtmt.hu/api/publication/33630893 ID - 33630893 AB - Feline idiopathic cystitis (FIC) is one of the most common urinary tract disorders in domestic cats. As stress is suggested to play a key role in the pathogenesis of FIC, the effects of norepinephrine (NE) as a stress mediator were investigated on a novel feline primary uroepithelial cell culture, serving as an in vitro model of the disease. The uroepithelial cells gained from the mucosa of the bladder of a euthanized cat were cultured for 6 days and were acutely exposed to NE (10, 100, and 1000 µM) for 1 h. NE increased the metabolic activity of the cultured cells and elevated the extracellular concentrations of the pro-inflammatory mediators interleukin-6 (IL-6) and stromal cell derived factor 1 (SDF-1), confirming that NE can trigger an inflammatory response in the uroepithelium. Cellular protein carbonyl levels were increased by NE exposure, while malondialdehyde and glucose regulated protein 78 concentrations remained unchanged, indicating that NE may provoke the oxidative damage of proteins without inducing lipid peroxidation or endoplasmic reticulum stress. Further, it can be strongly suggested that an acute NE challenge might diminish the barrier function of uroepithelial cells, as reflected by the decreased glycosaminoglycan concentration, claudin-4 protein expression, and reduced TER values of the NE-treated cell cultures. Based on these results, short-term NE exposure mimicking acute stress can provoke an inflammatory response and decrease the barrier integrity of cultured feline uroepithelial cells. Hence, it is highly expected that stress-associated NE release may play an important mediatory role in the pathogenesis of FIC. LA - English DB - MTMT ER -