TY - CONF AU - Reider, Balázs TI - Új prosztatarák diagnosztikai módszer fejlesztése T2 - Pannon Innovációs Nap PY - 2023 UR - https://m2.mtmt.hu/api/publication/34016892 ID - 34016892 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Hajba, László AU - Jeong, Sunkyung AU - Chung, Doo Soo AU - Guttman, András TI - Capillary Gel Electrophoresis of Proteins: Historical overview and recent advances JF - TRAC-TRENDS IN ANALYTICAL CHEMISTRY J2 - TRAC-TREND ANAL CHEM VL - 162 PY - 2023 SN - 0165-9936 DO - 10.1016/j.trac.2023.117024 UR - https://m2.mtmt.hu/api/publication/33707474 ID - 33707474 AB - This review summarizes the fundamental principles, basic methodologies, strength and weaknesses of capillary gel electrophoresis of proteins by providing both a short historical overview and highlighting new developments and applications in biopharmaceutical, biomedical as well as food and agriculture fields. The subsets of the method including native capillary gel electrophoresis, SDS capillary gel electrophoresis, capillary gel isoelectric focusing, capillary gel isotachophoresis and capillary affinity gel electrophoresis of proteins are all critically reviewed. Relevant protein labeling techniques are also addressed. LA - English DB - MTMT ER - TY - JOUR AU - Mirankó, Mirella AU - Tóth, Judit AU - Bartos, Csilla AU - Ambrus, Rita AU - Feczkó, Tivadar TI - Nano-Spray-Dried Levocetirizine Dihydrochloride with Mucoadhesive Carriers and Cyclodextrins for Nasal Administration JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 15 PY - 2023 IS - 2 PG - 13 SN - 1999-4923 DO - 10.3390/pharmaceutics15020317 UR - https://m2.mtmt.hu/api/publication/33573245 ID - 33573245 AB - Antihistamines such as levocetirizine dihydrochloride (LC) are commercially used in oral tablets and oral drops to reduce allergic symptoms. In this study, LC was nano-spray-dried using three mucoadhesive polymers and four cyclodextrin species to form composite powders for nasal administration. The product was composed of hydroxypropyl methylcellulose polymer, including LC as a zwitterion, after neutralization by NaOH, and XRD investigations verified its amorphous state. This and a sulfobutylated-beta-cyclodextrin sodium salt-containing sample showed crystal peaks due to NaCl content as products of the neutralization reaction in the solutions before drying. The average particle size of the spherical microparticles was between 2.42 and 3.44 µm, except for those containing a polyvinyl alcohol excipient, which were characterized by a medium diameter of 29.80 µm. The drug was completely and immediately liberated from all the samples at pH 5.6 and 32 °C; i.e., the carriers did not change the good dissolution behavior of LC. A permeability test was carried out by dipping the synthetic cellulose ester membrane in isopropyl myristate using modified horizontal diffusion cells. The spray-dried powder with β-cyclodextrin showed the highest permeability (188.37 µg/cm2/h), as this additive was the least hydrophilic. Products prepared with other cyclodextrins (randomly methylated-beta-cyclodextrin, sulfobutylated-beta-cyclodextrin sodium salt and (hydroxypropyl)-beta-cyclodextrin) showed similar or slightly higher penetration abilities than LC. Other polymer excipients resulted in lower penetration of the active agent than the pure LC. LA - English DB - MTMT ER - TY - JOUR AU - Gyebrovszki, Balázs AU - Ács, András AU - Szabó, Dániel AU - Auer, Felícia AU - Novozánszki, Soma AU - Rojkovich, Bernadette AU - Magyar, Anna (Biczókné) AU - Hudecz, Ferenc AU - Vékey, Károly AU - Drahos, László AU - Sármay, Gabriella TI - The Role of IgG Fc Region N-Glycosylation in the Pathomechanism of Rheumatoid Arthritis JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 10 SN - 1661-6596 DO - 10.3390/ijms23105828 UR - https://m2.mtmt.hu/api/publication/32840673 ID - 32840673 N1 - Department of Immunology, Eötvös Loránd University, Budapest, 1117, Hungary MS Proteomics Research Group, Research Centre for Natural Sciences, Eötvös Loránd Research Network, Budapest, 1117, Hungary Hevesy György PhD School of Chemistry, Faculty of Science, Eötvös Loránd University, Budapest, 1117, Hungary Translational Glycomics Research Group, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprém, 8200, Hungary Central Laboratory-Microbiology Profile, Molecular Department, National Institute of Hematology and Infectious Diseases, Central Hospital of Southern Pest, Budapest, 1097, Hungary Rheumatology Department III, Polyclinic of the Hospitaller Brothers of St. John of God, Budapest, 1027, Hungary ELKH-ELTE Research Group of Peptide Chemistry, Budapest, 1117, Hungary Export Date: 3 October 2022 Correspondence Address: Sármay, G.; Department of Immunology, Hungary; email: gabriella.sarmay@ttk.elte.hu AB - Anti-citrullinated protein antibodies (ACPAs) are involved in the pathogenesis of rheumatoid arthritis. N-glycosylation pattern of ACPA-IgG and healthy IgG Fc differs. The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNF alpha production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA. We isolated IgG from sera of healthy volunteers and RA patients, and purified ACPAs on a citrulline-peptide column. Immunocomplexes (IC) were formed by adding an F(ab)(2) fragment of anti-human IgG. U937 cells were used to monitor the binding of IC to Fc gamma R and to trigger TNF alpha release determined by ELISA. To analyze glycan profiles, control IgG and ACPA-IgG were digested with trypsin and the glycosylation patterns of glycopeptides were analyzed by determining site-specific N-glycosylation using nano-UHPLC-MS/MS. We found that both sialylation and galactosylation levels of ACPA-IgG negatively correlate with inflammation-related parameters such as CRP, ESR, and RF. Functional assays show that dimerized ACPA-IgG significantly enhances TNF alpha release in an Fc gamma RI-dependent manner, whereas healthy IgG does not. TNF alpha production inversely correlates with the relative intensities of the GO glycoform, which lacks galactose and terminal sialic acid moieties. LA - English DB - MTMT ER - TY - PAT AU - Járvás, Gábor AU - Reider, Balázs AU - Jankovics, Hajnalka AU - Vonderviszt, Ferenc AU - Guttman, András TI - Integrated method for urinary prostate specific antigen N-glycosylation profiling by capillary electrophoresis PY - 2022 UR - https://m2.mtmt.hu/api/publication/32637715 ID - 32637715 LA - English DB - MTMT ER - TY - PAT AU - Járvás, Gábor AU - Szigeti, Márton AU - Guttman, András AU - Chapman, Jeff TI - A Triple-Internal Standard Based Glycan Structural Assignment Method For Capillary Electrophoresis Analysis Of Carbohydrates CY - Country:10017(13) PY - 2021 UR - https://m2.mtmt.hu/api/publication/32915469 ID - 32915469 AB - A separation device receives a known or unknown glycan that is co-injected with three different oligomers maltooligosaccharide (MOL). A detector measures the separated glycan and the separated three different oligomers as intensity peaks that are a function of migration time. The migration times of a plurality of other oligomers of MOL are calculated from the migration times of the three different oligomers. Glucose unit (GU) values for the intensity peaks of the separated glycan are calculated by comparing their migration times to the calculated migration times of the plurality of other oligomers of MOL. The processor identifies the structure of the glycan by comparing the calculated GU values of the intensity peaks of the separated glycan to a database of GU values for known glycan structures. LA - English DB - MTMT ER - TY - JOUR AU - Reider, Balázs AU - Gacsi, Eszter AU - Jankovics, Hajnalka AU - Vonderviszt, Ferenc AU - Szarvas, Tibor AU - Guttman, András AU - Járvás, Gábor TI - Integrated workflow for urinary prostate specific antigen N-glycosylation analysis using sdAb partitioning and downstream capillary electrophoresis separation JF - ANALYTICA CHIMICA ACTA J2 - ANAL CHIM ACTA VL - 1184 PY - 2021 PG - 10 SN - 0003-2670 DO - 10.1016/j.aca.2021.338892 UR - https://m2.mtmt.hu/api/publication/32487497 ID - 32487497 N1 - Funding Agency and Grant Number: National Research, Development and Innovation OfficeNational Research, Development & Innovation Office (NRDIO) - Hungary [2018-2.1.17-TET-KR-2018-00010]; Hungarian Government; New National Excellence Program Hungarian Ministry of Human Capacities and the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences [UNKP-20-5]; Janos Bolyai Research Scholarship of the Hungarian Academy of SciencesHungarian Academy of Sciences; National Research, Development and Innovation Fund of Hungary [TKP2020-IKA-07, 2020-4.1.1-TKP2020] Funding text: The authors gratefully acknowledge the support from the National Research, Development and Innovation Office (2018-2.1.17-T?ET-KR-2018-00010) grants of the Hungarian Government. This work was also supported by the UNKP-20-5 New National Excellence Program Hungarian Ministry of Human Capacities and the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. This work was supported by the TKP2020-IKA-07 project financed under the 2020-4.1.1-TKP2020 Thematic Excellence Programme by the National Research, Development and Innovation Fund of Hungary. The stimulating discussion with Sandor Nagy, MD (Ferenc Csolnoky General Hospital) on medical aspects of prostate cancer is also highly appreciated. Finally, we appreciate the generous help of Noemi Kovacs regarding nanobody production. This is contribution #185 of the Horvath Csaba Memorial Laboratory of Bioseparation Sciences. AB - Prostate cancer represents the second highest malignancy rate in men in all cancer diagnoses worldwide. The development and progression of prostate cancer is not completely understood yet at molecular level, but it has been reported that changes in the N-glycosylation of prostate-specific antigen (PSA) occur during tumor genesis. In this paper we report on the development and implementation of a highthroughput capillary electrophoresis based glycan analysis workflow for urinary PSA analysis. The technology utilizes selective, high yield single domain antibody based PSA capture, followed by preconcentration and capillary electrophoresis coupled with laser-induced fluorescence detection, resulting in high resolution N-glycan profiles. Urinary PSA glycan profiles were compared to a commercially available PSA standard revealing differences in their a2,3-and a2,6-sialylated isomers, proving the excellent selectivity of the suggested workflow. This is important as sialylation classification plays an important role in the differentiation between indolent, significant and aggressive forms of prostate cancer. (c) 2021 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). LA - English DB - MTMT ER - TY - JOUR AU - Kasza, György AU - Stumphauser, Tímea AU - Bisztrán, Márk AU - Szarka, Györgyi Éva AU - Hegedüs, Imre AU - Nagy, Endre AU - Iván, Béla TI - Thermoresponsive Poly(N,N-diethylacrylamide-co-glycidyl methacrylate) Copolymers and Its Catalytically Active α-Chymotrypsin Bioconjugate with Enhanced Enzyme Stability JF - POLYMERS J2 - POLYMERS-BASEL VL - 13 PY - 2021 IS - 6 PG - 18 SN - 2073-4360 DO - 10.3390/polym13060987 UR - https://m2.mtmt.hu/api/publication/31929285 ID - 31929285 N1 - cited By 1 LA - English DB - MTMT ER - TY - JOUR AU - Jankovics, Hajnalka AU - Szekér, P. AU - Tóth, Éva AU - Kakasi, Balázs AU - Lábadi, Zoltán AU - Saftics, András AU - Kalas, Benjamin AU - Fried, Miklós AU - Petrik, Péter AU - Vonderviszt, Ferenc TI - Flagellin-based electrochemical sensing layer for arsenic detection in water JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 11 PY - 2021 IS - 1 PG - 10 SN - 2045-2322 DO - 10.1038/s41598-021-83053-y UR - https://m2.mtmt.hu/api/publication/31921234 ID - 31921234 LA - English DB - MTMT ER - TY - JOUR AU - Nagy, Endre AU - Hegedüs, Imre AU - Rehman, Danyal AU - Wei, Quantum J. AU - Ahdab, Yvana D. AU - Lienhard, John H. TI - The Need for Accurate Osmotic Pressure and Mass Transfer Resistances in Modeling Osmotically Driven Membrane Processes JF - MEMBRANES (BASEL) J2 - MEMBRANES-BASEL VL - 11 PY - 2021 IS - 2 PG - 34 SN - 2077-0375 DO - 10.3390/membranes11020128 UR - https://m2.mtmt.hu/api/publication/31886736 ID - 31886736 N1 - cited By 4 LA - English DB - MTMT ER -