TY - CONF AU - Tarjányi, Tamás AU - Bogár, Ferenc AU - Gajdács, Márió AU - Minárovits, János AU - Tóth, Zsolt ED - Baráth, Zoltán Lajos ED - Stájer, Anette ED - Madléna, Melinda ED - Matusovits, Danica ED - Kárpáti, Krisztina ED - Gajdács, Márió TI - Osszeointegráció molekuláris megközelítésben: kristályos és amorf titán-oxid felületekkel kölcsönható biomolekulák T2 - Szegedi Fogorvosnapok 2022. Szegedi Fogorvos Találkozó és Tudományos Konferencia, Magyar Gyermekfogászati és Fogszabályozási Társaság IX. Tóth Pál Vándorgyűlés PB - Szegedi Tudományegyetem Fogorvostudományi Kar C1 - Szeged SN - 9786150161211 PY - 2022 SP - 25 EP - 25 PG - 1 UR - https://m2.mtmt.hu/api/publication/33118459 ID - 33118459 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Szolomájer, János AU - Stráner, Pál AU - Kele, Zoltán AU - Tóth, Gábor AU - Perczel, András TI - Synthesis of the extracellular domain of GLP-1R by chemical and biotechnological approaches JF - RSC ADVANCES J2 - RSC ADV VL - 12 PY - 2022 IS - 37 SP - 24278 EP - 24287 PG - 10 SN - 2046-2069 DO - 10.1039/D2RA02784D UR - https://m2.mtmt.hu/api/publication/33071352 ID - 33071352 N1 - Department of Medical Chemistry, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, H-6720, Hungary MTA-ELTE Protein Model. Res. Group and Laboratory of Structural Chemistry and Biology, Pázmány P. stny. 1/A, Budapest, 1117, Hungary Laboratory of Structural Chemistry and Biology, Institute of Chemistry, ELTE Eötvös Loránd University, Pázmány P. stny. 1/A, Budapest, 1117, Hungary MTA-SZTE Biomimetic Research Group, University of Szeged, Szeged, H-6720, Hungary Export Date: 5 April 2023 CODEN: RSCAC Correspondence Address: Tóth, G.K.; Department of Medical Chemistry, Hungary; email: toth.gabor@med.u-szeged.hu Correspondence Address: Perczel, A.; MTA-ELTE Protein Model. Res. Group and Laboratory of Structural Chemistry and Biology, Pázmány P. stny. 1/A, Hungary; email: perczel@chem.elte.hu AB - The extracellular domain of the glucagon-like peptide-1 receptor, GLP-1R, is responsible for the binding of GLP-1, and a handful of additional agonists (such as exenatide, lixisenatide, and liraglutide) used daily for treating type II diabetes mellitus. Lead discovery and optimization, however, require binding studies, which, in turn, necessitate the total synthesis of GLP-1R, comprising 108 residues. A protein domain of 10–15 kDa size could be obtained either by expression in E. coli or by ligating solid-phase peptide synthesis (SPPS)-made fragments. However, direct overexpression fails to give a properly folded protein, as GLP-1R forms an inclusion body, which fails to refold due to improper disulfide pairing. Several bacterial strains, constructs, and fusion partners were probed and it was found that only co-expression with MBP gave a 3D-fold allowing the native disulfide bond pattern formation. Some fusion partners can act as covalently linked or in situ chaperones for guiding the refolding of GLP-1R toward success. Therefore, the bottleneck to preparing GPCR extracellular domains is the correct pairing of the Cys residues. As a proof-of-concept model, nGLP1-R was made by SPPS to form the purified full-length polypeptide chain, subjected to self-guided or spontaneous Cys pairing. However, the formation of correct SS-pairs was lagging behind any protocol in use support, and the bottleneck of large-scale protein production relies on the risky step of proper refolding, which is sometimes possible only if a suitable fusion partner effectively helps and catalysis of the correct disulfide formation. LA - English DB - MTMT ER - TY - JOUR AU - Váradi, Györgyi AU - Galgóczi, László Norbert AU - Tóth, Gábor TI - Rationally Designed Antimicrobial Peptides Are Potential Tools to Combat Devastating Bacteria and Fungi JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 11 PG - 4 SN - 1661-6596 DO - 10.3390/ijms23116244 UR - https://m2.mtmt.hu/api/publication/32863716 ID - 32863716 N1 - Funding Agency and Grant Number: Hungarian National Research, Development and Innovation Office-NKFIH [FK 134343, TKP2021-EGA-32] Funding text: L.G. was financed by the FK 134343 project of the Hungarian National Research, Development and Innovation Office-NKFIH, and G.V. and G.K.T. were supported by the TKP2021-EGA-32 fund of the Hungarian National Research, Development and Innovation Office-NKFIH. LA - English DB - MTMT ER - TY - JOUR AU - Váradi, Zoltán AU - Paragi, Gábor AU - Kupihár, Zoltán AU - Kele, Zoltán AU - Kovács, Lajos TI - Synthesis of Heterocycles and Nucleosides Forming Higher—Order Structures JF - CHEMISTRY PROCEEDINGS J2 - CHEM PROC VL - 8 PY - 2022 IS - 1 PG - 6 SN - 2673-4583 DO - 10.3390/ecsoc-25-11705 UR - https://m2.mtmt.hu/api/publication/32818711 ID - 32818711 LA - English DB - MTMT ER - TY - JOUR AU - Csóti, Ágota AU - del Carmen Nájera Meza, Rosby AU - Bogár, Ferenc AU - Tajti, Gábor AU - Szántó, Gábor Tibor AU - Varga, Zoltán AU - Gurrola, Georgina B. AU - Tóth, Gábor AU - Possani, Lourival D. AU - Panyi, György TI - sVmKTx, a transcriptome analysis-based synthetic peptide analogue of Vm24, inhibits Kv1.3 channels of human T cells with improved selectivity JF - BIOCHEMICAL PHARMACOLOGY J2 - BIOCHEMIC PHARMACOL VL - 199 PY - 2022 PG - 14 SN - 0006-2952 DO - 10.1016/j.bcp.2022.115023 UR - https://m2.mtmt.hu/api/publication/32803926 ID - 32803926 LA - English DB - MTMT ER - TY - JOUR AU - Váczi, Sándor AU - Barna, Lilla AU - Laczi, Krisztián AU - Tömösi, Ferenc AU - Rákhely, Gábor AU - Penke, Botond AU - Fülöp, Lívia AU - Bogár, Ferenc AU - Janáky, Tamás AU - Deli, Mária Anna AU - Mezei, Zsófia TI - Effects of sub-chronic, in vivo administration of sigma non-opioid intracellular receptor 1 ligands on platelet and aortic arachidonate cascade in rats JF - EUROPEAN JOURNAL OF PHARMACOLOGY J2 - EUR J PHARMACOL VL - 925 PY - 2022 PG - 10 SN - 0014-2999 DO - 10.1016/j.ejphar.2022.174983 UR - https://m2.mtmt.hu/api/publication/32801748 ID - 32801748 N1 - További támogatások: ÚNKP-20-3-SZTE-503; Richter Gedeon Nyrt. Centenáriumi Alapítvány 2020/K/21/2503 LA - English DB - MTMT ER - TY - JOUR AU - Simon, Márton AU - Bartus, Éva AU - Mag, Beáta Zsófia AU - Boros, Eszter AU - Roszjár, Lea AU - Gógl, Gergő AU - Travé, Gilles AU - Martinek, Tamás AU - Nyitray, László TI - Promiscuity mapping of the S100 protein family using a high-throughput holdup assay JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 12 PY - 2022 IS - 1 PG - 11 SN - 2045-2322 DO - 10.1038/s41598-022-09574-2 UR - https://m2.mtmt.hu/api/publication/32777033 ID - 32777033 N1 - These authors contributed equally: Márton A. Simon and Éva Bartus LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Liliána AU - Poór, Péter AU - Ördög, Attila AU - Váradi, Györgyi AU - Farkas, Attila AU - Papp, Csaba Gergő AU - Bende, Gábor AU - Tóth, Gábor AU - Rákhely, Gábor AU - Marx, Florentine AU - Galgóczi, László Norbert TI - The combination of Neosartorya (Aspergillus) fischeri antifungal proteins with rationally designed γ-core peptide derivatives is effective for plant and crop protection JF - BIOCONTROL J2 - BIOCONTROL VL - 67 PY - 2022 IS - 2 SP - 249 EP - 262 PG - 14 SN - 1386-6141 DO - 10.1007/s10526-022-10132-y UR - https://m2.mtmt.hu/api/publication/32655436 ID - 32655436 N1 - Export Date: 23 June 2022 CODEN: BOCOF AB - Plant pathogenic fungi are responsible for enormous crop losses worldwide. Overcoming this problem is challenging as these fungi can be highly resistant to approved chemical fungicides. There is thus a need to develop and introduce fundamentally new plant and crop protection strategies for sustainable agricultural production. Highly stable extracellular antifungal proteins (AFPs) and their rationally designed peptide derivatives (PDs) constitute feasible options to meet this challenge. In the present study, their potential for topical application to protect plants and crops as combinatorial biofungicides is supported by the investigation of two Neosartorya (Aspergillus) fischeri AFPs (NFAP and NFAP2) and their γ-core PDs. Previously, the biofungicidal potential of NFAP, its rationally designed γ-core PD (γNFAP-opt), and NFAP2 was reported. Susceptibility tests in the present study extended the in vitro antifungal spectrum of NFAP2 and its γ-core PD (γNFAP2-opt) to Botrytis, Cladosporium, and Fusarium spp. Besides, in vitro additive or indifferent interactions, and synergism were observed when NFAP or NFAP2 was applied in combination with γNFAP-opt. Except for γNFAP2-opt, the investigated proteins and peptides did not show any toxicity to tomato plant leaves. The application of NFAP in combination with γNFAP-opt effectively inhibited conidial germination, biofilm formation, and hyphal extension of the necrotrophic mold Botrytis cinerea on tomato plant leaves. However, the same combination only partially impeded the B. cinerea-mediated decay of tomato fruits, but mitigated the symptoms. Our results highlight the feasibility of using the combination of AFP and PD as biofungicide for the fungal infection control in plants and crops. LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Nikolett Alexandra AU - Katona, Gábor AU - Juhász, Ádám AU - Balogh, György Tibor AU - Csapó, Edit TI - Albumin-hyaluronic acid colloidal nanocarriers: Effect of human and bovine serum albumin for intestinal ibuprofen release enhancement JF - JOURNAL OF MOLECULAR LIQUIDS J2 - J MOL LIQ VL - 351 PY - 2022 PG - 9 SN - 0167-7322 DO - 10.1016/j.molliq.2022.118614 UR - https://m2.mtmt.hu/api/publication/32641654 ID - 32641654 N1 - Export Date: 2 May 2022 CODEN: JMLID Correspondence Address: Balogh, G.T.; Department of Pharmacodynamics and Biopharmacy, H-6720 Eötvös Str. 6, Hungary; email: balogh.gyorgy.tibor@szte.hu LA - English DB - MTMT ER - TY - JOUR AU - Wojnicki, Marek AU - Michorczyk, Beata AU - Wojtaszek, Konrad AU - Kutyła, Dawid AU - Kołczyk-Siedlecka, Karolina AU - Małecki, Stanisław AU - Wrzesińska, Angelika AU - Kozanecki, Marcin AU - Kwolek, Przemysław AU - Gajewska, Marta AU - Socha, Robert P. AU - Csapó, Edit AU - Escribà-Gelonch, Marc AU - Hessel, Volker TI - Zero waste, single step methods of fabrication of reduced graphene oxide decorated with gold nanoparticles JF - SUSTAINABLE MATERIALS AND TECHNOLOGIES J2 - SUSTAINABLE MATERIALS TECHNOLOGIES VL - 31 PY - 2022 PG - 15 SN - 2214-9929 DO - 10.1016/j.susmat.2022.e00387 UR - https://m2.mtmt.hu/api/publication/32589223 ID - 32589223 AB - This paper reports a novel approach to the use of carbon, in the form of reduced graphene oxide, as a reducing agent for Au(III) chloride complex ions. This approach allows fabrication of a composite material Au@GOr in a single-step process. The reduction of Au(III) complex ions was performed using high pressure, 50 bar, and high temperature, 250°C, reactor. The average diameter of obtained gold nanoparticles was below 3 nm. The advantage of the reduced graphene oxide as the reducing agent is its high surface area. This accelerates the reaction rate significantly. The greenness and sustainability of the process are assessed by green chemistry metrics and circularity indicators recently applied for the first time to a nanomaterial synthesis. As a key green metrics, atom economy (AE) measures the degree of the incorporation of reactant atoms into the final product and in the case of the research presented scoring 99%. LA - English DB - MTMT ER -