TY - JOUR AU - Gorgey, Eva AU - Tanics, Timea Judith Csabai AU - Bognar, Zoltan AU - Mikó, Éva AU - Szekeres, Júlia TI - [9h20-9h30] Immune checkpoint ligands expressed by embryo-derived extracellular vesicles JF - JOURNAL OF REPRODUCTIVE IMMUNOLOGY J2 - J REPROD IMMUNOL VL - 158 PY - 2023 PG - 1 SN - 0165-0378 DO - 10.1016/j.jri.2022.103520 UR - https://m2.mtmt.hu/api/publication/34212237 ID - 34212237 N1 - WoS:hiba:001061855500005 2023-12-31 23:29 cím nem egyezik LA - English DB - MTMT ER - TY - JOUR AU - Barbakadze, Tamar AU - Kvergelidze, Elisabed AU - Bátor, Judit AU - Szeberényi, József AU - Mikeladze, David TI - 3,5,3'-Triiodo-L-Thyronine Regulates Actin Cytoskeleton Dynamic in The Differentiated PC-12 Cells during Hypoxia through An αvβ3 Integrin. JF - CELL JOURNAL J2 - CELL J VL - 25 PY - 2023 IS - 4 SP - 247 EP - 254 PG - 8 SN - 2228-5806 DO - 10.22074/cellj.2022.557501.1059 UR - https://m2.mtmt.hu/api/publication/33937675 ID - 33937675 AB - Thyroid hormones are involved in the pathogenesis of various neurological disorders. Ischemia/hypoxia that induces rigidity of the actin filaments, which initiates neurodegeneration and reduces synaptic plasticity. We hypothesized that thyroid hormones via alpha-v-beta-3 (αvβ3) integrin could regulate the actin filament rearrangement during hypoxia and increase neuronal cell viability.In this experimental study, we analysed the dynamics of actin cytoskeleton according to the G/F actin ratio, cofilin-1/p-cofilin-1 ratio, and p-Fyn/Fyn ratio in differentiated PC-12 cells with/without T3 hormone (3,5,3'-triiodo-L-thyronine) treatment and blocking αvβ3-integrin-antibody under hypoxic conditions using electrophoresis and western blotting methods. We assessed NADPH oxidase activity under the hypoxic condition by the luminometric method and Rac1 activity using the ELISA-based (G-LISA) activation assay kit.The T3 hormone induces the αvβ3 integrin-dependent dephosphorylation of the Fyn kinase (P=0.0010), modulates the G/F actin ratio (P=0.0010) and activates the Rac1/NADPH oxidase/cofilin-1 (P=0.0069, P=0.0010, P=0.0045) pathway. T3 increases PC-12 cell viability (P=0.0050) during hypoxia via αvβ3 integrin-dependent downstream regulation systems.The T3 thyroid hormone may modulate the G/F actin ratio via the Rac1 GTPase/NADPH oxidase/ cofilin1signaling pathway and αvβ3-integrin-dependent suppression of Fyn kinase phosphorylation. LA - English DB - MTMT ER - TY - JOUR AU - Brandt, Barbara AU - Németh, Marica AU - Berta, Gergely AU - Szünstein, Máté AU - Heffer, Marija AU - Rauch, Tibor AU - Pap, Marianna TI - A Promising Way to Overcome Temozolomide Resistance through Inhibition of Protein Neddylation in Glioblastoma Cell Lines JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 9 PG - 16 SN - 1661-6596 DO - 10.3390/ijms24097929 UR - https://m2.mtmt.hu/api/publication/33788449 ID - 33788449 AB - There is no effective therapy for the lately increased incidence of glioblastoma multiforme (GBM)—the most common primary brain tumor characterized by a high degree of invasiveness and genetic heterogeneity. Currently, DNA alkylating agent temozolomide (TMZ) is the standard chemotherapy. Nevertheless, TMZ resistance is a major problem in the treatment of GBM due to numerous molecular mechanisms related to DNA damage repair, epigenetic alterations, cellular drug efflux, apoptosis-autophagy, and overactive protein neddylation. Low molecular weight inhibitors of NEDD8-activating enzyme (NAE), such as MLN4924, attenuate protein neddylation and present a promising low-toxicity anticancer agent. The aim of our study was to find an effective combination treatment with TMZ and MLN4924 in our TMZ-resistant GBM cell lines and study the effect of these combination treatments on different protein expressions such as O6-methylguanine methyltransferase (MGMT) and p53. The combination treatment successfully decreased cell viability and sensitized TMZ-resistant cells to TMZ, foreshadowing a new treatment strategy for GBM. LA - English DB - MTMT ER - TY - JOUR AU - Gábris, Fanni AU - Kiss, Gabriella AU - Szirmay, Balázs AU - Szomor, Árpád AU - Berta, Gergely AU - Jakus, Zoltán AU - Kellermayer, Zoltán AU - Balogh, Péter TI - Absence of Nkx2-3 induces ectopic lymphatic endothelial differentiation associated with impaired extramedullary stress hematopoiesis in the spleen. JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 11 PY - 2023 PG - 14 SN - 2296-634X DO - 10.3389/fcell.2023.1170389 UR - https://m2.mtmt.hu/api/publication/33775153 ID - 33775153 N1 - Department of Immunology and Biotechnology, University of Pécs Medical School, Pécs, Hungary Lymphoid Organogenesis Research Group, Szentágothai Research Center, University of Pécs, Pécs, Hungary Department of Laboratory Medicine, University of Pécs Medical School, Pécs, Hungary Department of Internal Medicine, University of Pécs Medical School, Pécs, Hungary Department of Medical Biology and Central Electron Microscope Laboratory, Medical School, University of Pécs, Pécs, Hungary Department of Physiology, Semmelweis University, Budapest, Hungary Export Date: 19 September 2023 Correspondence Address: Balogh, P.; Department of Immunology and Biotechnology, Hungary; email: balogh.peter@pte.hu AB - The red and white pulps as two main parts of the spleen are arranged around distinct types of vasculature, and perform significantly different functions in both humans and mice. Previous observations indicated a profound alteration of the local vessel specialization in mice lacking Nkx2-3 homeodomain transcription factor, including contradictory results suggesting presence of an ectopic lymphatic vascular structure. Furthermore, how the absence of Nkx2-3 and the consequential changes in endothelial components affect the extramedullary hematopoietic activity restricted to the splenic red pulp is unknown. In this work, we investigated the role of Nkx2-3 homeodomain transcription factor as a major morphogenic determinant for vascular specification, and its effect in the extramedullary hematopoiesis following acute blood loss and pharmacological stimulation of megakaryocyte differentiation after treatment with thrombopoietin-receptor mimetic Romiplostim. We found that, in mice lacking Nkx2-3, Prox1-positive lymphatic capillaries containing gp38/CD31 double positive lymphatic endothelial cells develop, arranged into an extensive meshwork, while the Clever1-positive venous segments of red pulp blood vasculature are absent. This lymphatic endothelial shift is coupled with a severely compromised splenic erythropoiesis and a significantly reduced splenic megakaryocyte colony formation following Romiplostim treatment in mice lacking Nkx2-3. These findings indicate that the shift of microvascular patterning in the absence of Nkx2-3 includes the emergence of ectopic Prox1-positive lymphatic vessels, and that this pivoting towards lymph node-like vascular patterning is associated with an impaired reserve hematopoietic capacity of the splenic red pulp. LA - English DB - MTMT ER - TY - JOUR AU - Balogh, Bálint AU - Vecsernyés, Mónika AU - Stayer-Harci, Alexandra AU - Berta, Gergely AU - Tarjányi, Oktávia AU - Sétáló, György (ifj.) TI - Urocortin stimulates the ERK1/2 signaling pathway and the proliferation of HeLa cells via CRF receptor 1 JF - FEBS OPEN BIO J2 - FEBS OPEN BIO VL - 13 PY - 2023 IS - 5 SP - 818 EP - 832 PG - 15 SN - 2211-5463 DO - 10.1002/2211-5463.13602 UR - https://m2.mtmt.hu/api/publication/33728557 ID - 33728557 AB - Corticotropin-releasing factor (CRF) stimulates adrenocorticotropic hormone (ACTH) secretion from the pituitary gland and is an essential regulator of the hypothalamic-pituitary-adrenocortical axis. Isoforms of CRF receptor are known to mediate the effects of urocortin stress ligands on the regulation of stress responses, anxiety, and feeding behavior; however, urocortin stress ligands also influence cell proliferation. In view of the tumor-promoting capacity of prolonged stress, here we investigated (a) the effect of urocortin on cell proliferative signaling via extracellular signal-regulated kinase 1/2, (b) the expression and cellular distribution of the specific CRF receptor isoforms, and (c) the intracellular localization of phosphorylated ERK1/2 in HeLa cells. Stimulation of cell proliferation was observed in the presence of 10 nm urocortin. Our data also suggest that MAP kinase MEK, the transcription factors E2F-1 and p53, and PKB/Akt are involved in this process. These findings may have therapeutic relevance for the targeted treatment of various malignancies. LA - English DB - MTMT ER - TY - JOUR AU - Horváth, Marianna AU - Kovács, Tamás AU - Kun, József AU - Gyenesei, Attila AU - Damjanova, Ivelina AU - Tigyi, Zoltán AU - Schneider, György TI - Virulence Characteristics and Molecular Typing of Carbapenem-Resistant ST15 Klebsiella pneumoniae Clinical Isolates, Possessing the K24 Capsular Type JF - ANTIBIOTICS J2 - ANTIBIOTICS-BASEL VL - 12 PY - 2023 IS - 3 PG - 22 SN - 2079-6382 DO - 10.3390/antibiotics12030479 UR - https://m2.mtmt.hu/api/publication/33728546 ID - 33728546 AB - Klebsiella pneumoniae is an opportunistic pathogen that frequently causes nosocomial and community-acquired (CA) infections. Until now, a limited number of studies has been focused on the analyses of changes affecting the virulence attributes. Genotypic and phenotypic methods were used to characterise the 39 clinical K. pneumoniae isolates; all belonged to the pan-drug resistant, widespread clone ST 15 and expressed the K24 capsule. PFGE has revealed that the isolates could be divided into three distinct genomic clusters. All isolates possessed allS and uge genes, known to contribute to the virulence of K. pneumoniae and 10.25% of the isolates showed hypermucoviscosity, 94.87% produced type 1 fimbriae, 92.3% produced type 3 fimbriae, and 92.3% were able to produce biofilm. In vivo persistence could be supported by serum resistance 46.15%, enterobactin (94.87%) and aerobactin (5.12%) production and invasion of the INT407 and T24 cell lines. Sequence analysis of the whole genomes of the four representative strains 11/3, 50/1, 53/2 and 53/3 has revealed high sequence homology to the reference K. pneumoniae strain HS11286. Our results represent the divergence of virulence attributes among the isolates derived from a common ancestor clone ST 15, in an evolutionary process that occurred both in the hospital and in the community. LA - English DB - MTMT ER - TY - JOUR AU - Ábrahám, Hajnalka AU - Kojima, Hisae AU - Götzer, Katalin AU - Molnár, Abigél AU - Tornóczky, Tamás AU - Seress, László TI - Development of parvalbumin-immunoreactive neurons in the postnatal human hippocampal formation JF - FRONTIERS IN NEUROANATOMY J2 - FRONT NEUROANAT VL - 17 PY - 2023 PG - 14 SN - 1662-5129 DO - 10.3389/fnana.2023.1058370 UR - https://m2.mtmt.hu/api/publication/33667143 ID - 33667143 N1 - Department of Medical Biology and Central Electron Microscopic Laboratory, University of Pécs Medical School, Pécs, Hungary Center for Neuroscience, University of Pécs, Pécs, Hungary Institute for the Psychology of Special Needs, Bárczi Gusztáv Faculty of Special Needs Education, Eötvös Loránd University, Budapest, Hungary Department of Pathology, University of Pécs Medical School, Pécs, Hungary Export Date: 4 December 2023 Correspondence Address: Ábrahám, H.; Department of Medical Biology and Central Electron Microscopic Laboratory, Hungary; email: hajnalka.abraham@aok.pte.hu AB - Introduction: Parvalbumin (PV) is a calcium-binding protein present in fast-spiking GABAergic neurons, such as basket and axo-axonic cells. Previous studies in non-human primates reported prenatal expression of PV in the temporal archicortex including entorhinal cortex and hippocampal formation. In contrast, PV-immunoreactivity was observed only postnatally in the human entorhinal cortex. Regarding PV expression in the human hippocampal formation, no information is available. Methods: In this study, the neurochemical maturation of PV-immunoreactive interneurons was studied in the postnatal developing human hippocampal formation. Results: Before birth, no PV-immunoreactive neurons could be detected in the human hippocampus. At birth, only a few PV-immunoreactive neurons were visible in Ammon's horn. The first PV-immunoreactive cells in the hilus of the dentate gyrus appeared at the age of 1 month. Even at the age of 5 months, only a few PV-immunopositive cells were present in the dentate hilus. The number of cells and their dendritic and axonal arborization in Ammon's horn and in the dentate gyrus gradually increased with age. Even at the age of 2 years, dendritic tree and axons of PV-immunoreactive neurons were less complex than can be seen in 8 and 11 years old children. Discussion: Our results showed that long-lasting maturation of PV-immunoreactive interneurons follows the developmental sequence of the subfields of the human hippocampal formation and provides further morphological evidence for the long-lasting functional maturation of the human cortex. LA - English DB - MTMT ER - TY - JOUR AU - Boros, Melinda AU - Sóki, Noémi AU - Molnár, A. AU - Ábrahám, Hajnalka TI - Morphological study of the postnatal hippocampal development in the TRPV1 knockout mice JF - TEMPERATURE J2 - TEMPERATURE VL - 10 PY - 2023 IS - 1 SP - 102 EP - 120 PG - 19 SN - 2332-8940 DO - 10.1080/23328940.2023.2167444 UR - https://m2.mtmt.hu/api/publication/33647817 ID - 33647817 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - GEN AU - Ábrahám, Hajnalka AU - Boros, Melinda AU - Sóki, Noémi AU - Abigél, Molnár TI - Postnatal development of the hippocampal formation in the TRPV1 knockout mice PY - 2023 UR - https://m2.mtmt.hu/api/publication/33629363 ID - 33629363 LA - English DB - MTMT ER - TY - JOUR AU - Biró, Ede AU - Sommer, Gerhard AU - Leitinger, Gerd AU - Ábrahám, Hajnalka AU - Kardos, Dániel József AU - Oberritter, Zsolt AU - Saxena, Amulya K TI - Ultrastructural changes in esophageal tissue undergoing stretch tests with possible impact on tissue engineering and long gap esophageal repairs performed under tension. JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 8 SN - 2045-2322 DO - 10.1038/s41598-023-28894-5 UR - https://m2.mtmt.hu/api/publication/33625185 ID - 33625185 AB - Esophageal biomechanical studies are being performed to understand structural changes resulting from stretches during repair of esophageal atresias as well as to obtain biomechanical values for tissue-engineered esophagus. The present study offers insights into ultrastructural changes after stretching of the ovine esophagus using uniaxial stretch tests. In vitro uniaxial stretching was performed on esophagi (n = 16) obtained from the abattoir within 4-6 h of 1-month-old lambs. Esophagi were divided into 4 groups (4 esophagi/group): control, Group1 (G1), Group2 (G2), Group3 (G3) stretched to 20%, 30% and 40% of their original length respectively. Force and lengthening were measured with 5 cycles performed on every specimen. Transmission electron microscopic (TEM) studies were performed on the 4 groups. During observational TEM study of the control group there were no significant differences in muscle cell structure or extracellular matrix. In all stretched groups varying degrees of alterations were identified. The degree of damage correlated linearly with the increasing level of stretch. Distance between the cells showed significant difference between the groups (control (μ = 0.41 μm, SD = 0.26), G1 (μ = 1.36 μm, SD = 1.21), G2 (μ = 2.8 μm, SD = 1.83), and G3 (μ = 3.01 μm, SD = 2.06). The diameter of the cells (control μ = 19.87 μm, SD = 3.81; G1 μ = 20.38 μm, SD = 4.45; G2 μ = 21.7 μm, SD = 6.58; G3 μ = 24.48 μm, SD = 6.69) and the distance between myofibrils (control μ = 0.23 μm, SD = 0.08; G1 μ = 0.27 μm, SD = 0.08; G2 μ = 0.4 μm, SD = 0.15; G3 μ = 0.61 μm, SD = 0.2) were significantly different as well ( p < 0.05 was considered to be significant). Esophageal stretching > 30% alters the regular intracellular and extracellular structure of the esophageal muscle and leads to disruption of intra- and extracellular bonds. These findings could provide valuable insights into alterations in the microscopic structure of the esophagus in esophageal atresias repaired under tension as well as the basis for mechanical characterization for tissue engineering of the esophagus. LA - English DB - MTMT ER -