TY - GEN AU - Schvarcz, Csaba András AU - Lea, Danics AU - Leroy Viana, Pedro Henrique AU - Benyó, Zoltán AU - Kaucsár, Tamás AU - Hamar, Péter TI - Modulated electro hyperthermia inhibits tumor progression and optimizes systemic immune-response in a triple negative mouse breast cancer model PY - 2021 PG - 1 UR - https://m2.mtmt.hu/api/publication/32850102 ID - 32850102 AB - Introduction: Effective therapy of triple-negative breast cancer (TNBC) has not yet been achieved. Modulated electro-hyperthermia (mEHT) is a novel therapeutic option, based on the selective heating and energy transfer to the tumor tissue by electromagnetic field. Aims: Our aim was to investigate the effects of repeated mEHT treatment in a triple-negative mammary carcinoma bearing mouse model. Method: 4T07 cells were inoculated orthotopically in female BALB/c mice. Tumor growth was monitored by caliper and ultrasound (Phillips Sonos 5500). Treatments started 7 days after inoculation and were repeated 3 or 5 times, every other day. Tumor and blood samples were taken 24 hours after last treatment. Tumor destruction rate was evaluated on H&E and cleaved caspase-3 stained immunohistochemical sections. HSP70 and Ki67 expression were analyzed on immunohistochemical sections. Circulating immune cells (CD4+, CD8+ T lymphocytes, granulocytes, myeloid-derived suppressor cells (MDSCs)) were analyzed with flow cytometry. Expression of granulocyte-colony stimulating factor (G-CSF) mRNA, isolated from the tumor was evaluated with qPCR. Results: Ratio of both CD4+ and CD8+ lymphocytes increased significantly after repeated treatments. Ratio of granulocytes, MDSCs in the circulation and the G-CSF expression of tumor cells decreased significantly after repeated mEHT treatments. mEHT caused 6.1 fold higher HSP70 elevation in the tumor tissue, compared to the sham group (p<0.001). Tumor size significantly decreased (tumor weight sham: 288.3±58.1 mg vs mEHT: 85.3±21.3 mg, p<0.05) with the elevation of tissue destruction and reduction of Ki67 positive nuclei number (sham: 2823.4±211.9 pcs/mm2 vs mEHT: 1736.7±315.3 pcs/mm2, p<0.05) in treated tumors. Conclusion: Our findings suggest, that repeated mEHT could optimize systemic immune-response with the elevation of effector T lymphocytes and the decrease of tumor-promoting MDSCs. The treatment reduced tumor growth with heat-shock-mediated tissue destruction and impaired cell proliferation. Thus, mEHT could be a possible alternative adjuvant therapeutic strategy for TNBC cancer patients. LA - English DB - MTMT ER - TY - JOUR AU - Lőrincz, András AU - Mihály, Judith AU - Wacha, András Ferenc AU - Németh, Csaba AU - Besztercei, Balázs AU - Gyulavári, Pál AU - Varga, Zoltán AU - Peták, István AU - Bóta, Attila TI - Combination of multifunctional ursolic acid with kinase inhibitors for anti-cancer drug carrier vesicles JF - MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS J2 - MAT SCI ENG C-MATER VL - 131 PY - 2021 PG - 11 SN - 0928-4931 DO - 10.1016/j.msec.2021.112481 UR - https://m2.mtmt.hu/api/publication/32461679 ID - 32461679 N1 - Research Centre for Natural Sciences - Eötvös Loránd Research Network, Institute of Materials and Environmental Chemistry, Research Group of Biological Nanochemistry, Magyar tudósok boulevard 2, Budapest, 1117, Hungary Semmelweis University, Institute of Clinical Experimental Research, Tűzoltó street 37-47, Budapest, 1094, Hungary Semmelweis University, Pathobiochemistry Research Group, Tűzoltó street 37-47, Budapest, 1094, Hungary University of Illinois at Chicago, Department of Biopharmaceutical Sciences, 833 S. Wood street, Chicago, IL 60612, United States Oncompass Medicine Ltd., Retek street 34, Budapest, 1024, Hungary Semmelweis University, Department of Pharmacology and Pharmacotherapy, Nagyvárad square 4, Budapest, 1089, Hungary Export Date: 26 October 2021 Correspondence Address: Mihály, J.; Research Centre for Natural Sciences - Eötvös Loránd Research Network, Magyar tudósok boulevard 2, Hungary; email: mihaly.judith@ttk.hu LA - English DB - MTMT ER - TY - JOUR AU - Merkely, Petra AU - Bakos, Marcell AU - Bányai, Bálint Péter AU - Monori-Kiss, Anna AU - Horvath, Eszter Mária AU - Bognár, Judit AU - Benkő, Rita AU - Oláh, Attila AU - Radovits, Tamás AU - Merkely, Béla Péter AU - Ács, Nándor AU - Nádasy, György László AU - Török, Marianna AU - Várbíró, Szabolcs TI - Sex Differences in Exercise-Training-Related Functional and Morphological Adaptation of Rat Gracilis Muscle Arterioles JF - FRONTIERS IN PHYSIOLOGY J2 - FRONT PHYSIOL VL - 12 PY - 2021 PG - 13 SN - 1664-042X DO - 10.3389/fphys.2021.685664 UR - https://m2.mtmt.hu/api/publication/32118625 ID - 32118625 N1 - Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary Department of Physiology, Semmelweis University, Budapest, Hungary Institute of Clinical Experimental Research, Semmelweis University, Budapest, Hungary Heart and Vascular Center, Semmelweis University, Budapest, Hungary Cited By :3 Export Date: 3 May 2024 Correspondence Address: Török, M.; Department of Obstetrics and Gynecology, Hungary; email: torok.marianna91@gmail.com Chemicals/CAS: testosterone, 58-22-0 Funding text 1: This work was supported by grants from the National Research, Development and Innovation Office of Hungary (K120277, K32019, and K135076). This project was also supported by a grant from the National Development Agency of Hungary (TÁMOP-4.2.2/B-10/1-2010-0013) and Semmelweis Science and Innovation Fund (STIA-KF-17 for SV). The Project No. NVKP_16-1–2016-0017 (“National Heart Program”) was implemented with the support provided by the National Research, Development and Innovation Fund of Hungary, financed under the NVKP_16 funding scheme. The research was financed by the Thematic Excellence Program (2020-4.1.1.-TKP2020) of the Ministry for Innovation and Technology in Hungary within the framework of the Therapeutic Development and Bioimaging thematic programs of Semmelweis University. Further support was provided by the Hungarian Hypertension Society and the Dean of the Medical Faculty, Semmelweis University. Funding text 2: The expert technical assistance of Henriett Bir?, T?mea Fischinger, D?ra Juh?sz, G?bor Fritz, R?ka Eszter Sziva, Borb?la P?terffy, Attila J?svai, and Ildik? Oravecz was gratefully acknowledged. Funding. This work was supported by grants from the National Research, Development and Innovation Office of Hungary (K120277, K32019, and K135076). This project was also supported by a grant from the National Development Agency of Hungary (T?MOP-4.2.2/B-10/1-2010-0013) and Semmelweis Science and Innovation Fund (STIA-KF-17 for SV). The Project No. NVKP_16-1?2016-0017 (?National Heart Program?) was implemented with the support provided by the National Research, Development and Innovation Fund of Hungary, financed under the NVKP_16 funding scheme. The research was financed by the Thematic Excellence Program (2020-4.1.1.-TKP2020) of the Ministry for Innovation and Technology in Hungary within the framework of the Therapeutic Development and Bioimaging thematic programs of Semmelweis University. Further support was provided by the Hungarian Hypertension Society and the Dean of the Medical Faculty, Semmelweis University. AB - Background: The cardiovascular effects of training have been widely investigated; however, few studies have addressed sex differences in arteriolar adaptation. In the current study, we examined the adaptation of the gracilis arterioles of male and female rats in response to intensive training. Methods: Wistar rats were divided into four groups: male exercise (ME) and female exercise (FE) animals that underwent a 12-week intensive swim-training program (5 days/week, 200 min/day); and male control (MC) and female control (FC) animals that were placed in water for 5 min daily. Exercise-induced cardiac hypertrophy was confirmed by echocardiography. Following the training, the gracilis muscle arterioles were prepared, and their biomechanical properties and functional reactivity were tested, using pressure arteriography. Collagen and smooth muscle remodeling were observed in the histological sections. Results: Left ventricular mass was elevated in both sexes in response to chronic training. In the gracilis arterioles, the inner radius and wall tension increased in female animals, and the wall thickness and elastic modulus were reduced in males. Myogenic tone was reduced in the ME group, whereas norepinephrine-induced vasoconstriction was elevated in the FE group. More pronounced collagen staining was observed in the ME group than in the MC group. Relative hypertrophy and tangential stress of the gracilis arterioles were higher in females than in males. The direct vasoconstriction induced by testosterone was lower in females and was reduced as an effect of exercise in males. Conclusion: The gracilis muscle arteriole was remodeled as a result of swim training, and this adaptation was sex dependent. LA - English DB - MTMT ER - TY - JOUR AU - Török, Marianna AU - Merkely, Petra AU - Monori-Kiss, Anna AU - Horvath, Eszter Mária AU - Sziva, Réka Eszter AU - Péterffy, Borbála AU - Jósvai, Attila AU - Sayour, Alex Ali AU - Oláh, Attila AU - Radovits, Tamás AU - Merkely, Béla Péter AU - Ács, Nándor AU - Nádasy, György László AU - Várbíró, Szabolcs TI - Correction to: Network analysis of the left anterior descending coronary arteries in swim-trained rats by an in situ video microscopic technique JF - BIOLOGY OF SEX DIFFERENCES J2 - BIOL SEX DIFFER VL - 12 PY - 2021 IS - 1 PG - 2 SN - 2042-6410 DO - 10.1186/s13293-021-00385-0 UR - https://m2.mtmt.hu/api/publication/32079323 ID - 32079323 N1 - Marianna Török and Petra Merkely contributed equally to this work and are considered first authors LA - English DB - MTMT ER - TY - JOUR AU - Török, Marianna AU - Merkely, Petra AU - Monori-Kiss, Anna AU - Horvath, Eszter Mária AU - Sziva, Réka Eszter AU - Péterffy, Borbála AU - Jósvai, Attila AU - Sayour, Alex Ali AU - Oláh, Attila AU - Radovits, Tamás AU - Merkely, Béla Péter AU - Ács, Nándor AU - Nádasy, György László AU - Várbíró, Szabolcs TI - Network analysis of the left anterior descending coronary arteries in swim-trained rats by an in situ video microscopic technique JF - BIOLOGY OF SEX DIFFERENCES J2 - BIOL SEX DIFFER VL - 12 PY - 2021 IS - 1 PG - 17 SN - 2042-6410 DO - 10.1186/s13293-021-00379-y UR - https://m2.mtmt.hu/api/publication/32037670 ID - 32037670 N1 - Marianna Török and Petra Merkely contributed equally to this work and are considered first authors LA - English DB - MTMT ER - TY - JOUR AU - Török, Marianna AU - Horvath, Eszter Mária AU - Monori-Kiss, Anna AU - Pál, Éva AU - Gerszi, Dóra AU - Merkely, Petra AU - Sayour, Alex Ali AU - Mátyás, Csaba AU - Oláh, Attila AU - Radovits, Tamás AU - Merkely, Béla Péter AU - Ács, Nándor AU - Nádasy, György László AU - Várbíró, Szabolcs TI - Chronic swimming training resulted in more relaxed coronary arterioles in male and enhanced vasoconstrictor ability in female rats JF - JOURNAL OF SPORTS MEDICINE AND PHYSICAL FITNESS J2 - J SPORT MED PHYS FIT VL - 61 PY - 2021 IS - 3 SP - 489 EP - 496 PG - 8 SN - 0022-4707 DO - 10.23736/S0022-4707.20.11316-1 UR - https://m2.mtmt.hu/api/publication/31395770 ID - 31395770 N1 - Ellenőrizve teljes szöveg alapján GT AB - Exercise training is associated with hypertrophy of left ventricle (LV). The aim of the present study is to evaluate sex differences in the adaptation of the coronary contractile function in physiological left ventricular hypertrophy induced by long-term swim training.Thirty-two Wistar rats were randomly divided into 4 groups: exercised male (ExM), exercised female (ExF), untrained control male (CoM), and untrained control female (CoF). The trained animals underwent a 12-week-long swim training program. After finishing the training program, LV morphology and function were checked by echocardiography. The spontaneous tone, thromboxane (TxA2) agonistinduced vascular contractility and non-endothelial dilatation of the isolated intramural coronary resistance artery were examined by pressure microangiometry. The thromboxane receptor (TxA2R) protein expression in the wall of coronary arteries was examined using immunohistochemistry.The LV mass index was significantly higher in the ExM and ExF groups, furthermore the LV mass index was significantly higher in female than in male animals. ExM animals had lower spontaneous tone than ExF. TxA2 agonistinduced tone was raised only in ExF animals. The resistance coronary artery of exercised male animals had a significantly lower level of TxA2R positivity compared to exercised females.Both sex broaden their range of contractility following chronic swimming, but the vessel tone shifted toward contraction in exercised female rats, while these values shifted toward relaxation in males. These observations underline the significance of identifying potential gender differences in the chronic exercise-induced coronary vascular remodelling in human athletes. LA - English DB - MTMT ER - TY - JOUR AU - Boel, Annekatrien AU - Veszelyi, Krisztina Nóra AU - Németh, Csilla Emese AU - Beyens, Aude AU - Willaert, Andy AU - Coucke, Paul AU - Callewaert, Bert AU - Margittai, Éva TI - Arterial Tortuosity Syndrome: An Ascorbate Compartmentalization Disorder? JF - ANTIOXIDANTS & REDOX SIGNALING J2 - ANTIOXID REDOX SIGNAL VL - 34 PY - 2021 IS - 11 SP - 875 EP - 889 PG - 15 SN - 1523-0864 DO - 10.1089/ars.2019.7843 UR - https://m2.mtmt.hu/api/publication/30972609 ID - 30972609 AB - Significance: Cardiovascular disorders are the most important cause of morbidity and mortality in the Western world. Monogenic developmental disorders of the heart and vessels are highly valuable to study the physiological and pathological processes in cardiovascular system homeostasis. The arterial tortuosity syndrome (ATS) is a rare, autosomal recessive connective tissue disorder showing lengthening, tortuosity, and stenosis of the large arteries, with a propensity for aneurysm formation. In histopathology, it associates with fragmentation and disorganization of elastic fibers in several tissues, including the arterial wall. ATS is caused by pathogenic variants in SLC2A10 encoding the facilitative glucose transporter (GLUT)10. Critical Issues: Although several hypotheses have been forwarded, the molecular mechanisms linking disrupted GLUT10 activity with arterial malformations are largely unknown. Recent Advances: The vascular and systemic manifestations and natural history of ATS patients have been largely delineated. GLUT10 was identified as an intracellular transporter of dehydroascorbic acid, which contributes to collagen and elastin cross-linking in the endoplasmic reticulum, redox homeostasis in the mitochondria, and global and gene-specific methylation/hydroxymethylation affecting epigenetic regulation in the nucleus. We revise here the current knowledge on ATS and the role of GLUT10 within the compartmentalization of ascorbate in physiological and diseased states. Future Directions: Centralization of clinical, treatment, and outcome data will enable better management for ATS patients. Establishment of representative animal disease models could facilitate the study of pathomechanisms underlying ATS. This might be relevant for other forms of vascular dysplasia, such as isolated aneurysm formation, hypertensive vasculopathy, and neovascularization. Antioxid. Redox Signal. 00, 000-000. LA - English DB - MTMT ER - TY - JOUR AU - Jósvai, Attila AU - Török, Marianna AU - Mátrai, Máté AU - Hetthéssy, Judit AU - Monori-Kiss, Anna AU - Makk, Jennifer AU - Székács, Béla AU - Nádasy, György László AU - Várbíró, Szabolcs TI - Effects of Testosterone Deficiency and Angiotensin II-Induced Hypertension on the Biomechanics of Intramural Coronary Arteries JF - JOURNAL OF SEXUAL MEDICINE J2 - J SEX MED VL - 17 PY - 2020 IS - 12 SP - 2322 EP - 2330 PG - 9 SN - 1743-6095 DO - 10.1016/j.jsxm.2020.09.003 UR - https://m2.mtmt.hu/api/publication/31637466 ID - 31637466 N1 - These authors contributed equally to this work: Béla Székács, György L. Nádasy, and Szabolcs Várbíró Ellenőrizve teljes szöveg alapján GT AB - Andropause and hypertension also increase the risk of coronary artery damage.To investigate the effect of testosterone deficiency and hypertension on intramural coronary vessels.4 groups of 8-week-old Sprague-Dawley rats were studied: control male (Co, n=10), orchidectomized male (OCT, n=13), angiotensin (AII) hypertensive male (AII, n=10), and AII hypertensive and OCT (AII + OCT, n=8). Surgical orchidectomy was performed, and an osmotic minipump was inserted for chronic angiotensin II infusion (100 ng/min/kg). After 4 weeks, spontaneous tone and biomechanical properties of the intramural coronary resistance artery were investigated in vitro, by pressure microarteriography.Morphology and biomechanics of the intramural coronaries were evaluated: the outer diameter, wall thickness-to-lumen diameter ratio, and tangential wall stress in the contracted and relaxed states.The outer diameter was reduced in OCT and AII + OCT groups (on 50 mmHg 315 ± 20 Co; 237 ± 21 OCT; 291 ± 16 AII, and 166 ± 12 μm AII + OCT). The increased wall thickness-to-lumen diameter ratio resulted in lower tangential wall stress in AII + OCT rats (on 50 mmHg 19 ± 2 Co; 24 ± OCT; 26 ± 5 AII, and 9 ± 1 kPa AII + OCT). Spontaneous tone was increased in the hypertensive rats (AII and AII + OCT groups) (on 50 mmHg 7.7 ± 1.8 Co; 6.1 ± 1.4 OCT; 14.5 ± 3.0 AII, and 17.4 ± 4.1 % AII + OCT).Andropause alone can be considered as a cardiovascular risk factor that will further exacerbate vascular damage in hypertension.A limitation of our study is that it was performed on relatively young rats, and the conclusions might not apply to coronary remodelling in older animals with slower adaptation processes.Testosterone deficiency and hypertension damage the mechanical adaptation of the vessel wall additively: double noxa caused inward eutrophic remodeling and increased tone. Jósvai A, Török M, Mátrai M, et al. Effects of Testosterone Deficiency and Angiotensin II-Induced Hypertension on the Biomechanics of Intramural Coronary Arteries. J Sex Med 2020;XX:XXX-XXX. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Adrienn AU - Lénárt, Lilla AU - Hodrea, Judit AU - Besztercei, Balázs AU - Kovács, Illés AU - Szabó, Attila AU - Fekete, Andrea TI - Protective role of Sigma-1 receptor in corneal fibrosis JF - ORVOSKÉPZÉS J2 - ORVOSKÉPZÉS VL - 95 PY - 2020 IS - 1 SP - 118 EP - 118 PG - 1 SN - 0030-6037 UR - https://m2.mtmt.hu/api/publication/31599670 ID - 31599670 LA - English DB - MTMT ER - TY - CHAP AU - Szász, Attila Marcell AU - Árkosy, Péter AU - Arrojo, EE AU - Bakacs, T AU - Balogh, A AU - Barich, J AU - Borbényi, Erika AU - Chi, KH AU - Csoszi, T AU - Daniilidis, L AU - Douwes, F AU - Eller, YG AU - Fiorentini, G AU - Forika, Gertrud AU - Herzog, A AU - Kleef, R AU - Krenács, Tibor AU - Lee, SY AU - Minnaar, CA AU - Ou, J AU - Pang, CLK AU - Papastravrou, A AU - Parmar, G AU - Szentmártoni, Gyöngyvér AU - Szigeti, Gyula Péter AU - Van, Gool S AU - Wust, P AU - Dank, Magdolna ED - Szász, András TI - Guidelines for Local Hyperthermia Treatment in Oncology T2 - Challenges and Solutions of Oncological Hyperthermia PB - Cambridge Scholars Publishing CY - Newcastle upon Tyne SN - 1527548171 PY - 2020 SP - 32 EP - 71 PG - 30 UR - https://m2.mtmt.hu/api/publication/31359099 ID - 31359099 LA - English DB - MTMT ER -