TY - JOUR AU - Hassan, Alharith A. A. AU - Sovány, Tamás AU - Pamlényi, Krisztián AU - Deák, Martin AU - Varga, Viktória AU - Csapó, Edit AU - Regdon, Géza (ifj.) AU - Pannonhalminé Csóka, Ildikó AU - Kristó, Katalin TI - QbD Approach-Based Preparation and Optimization of Hydrophobic Ion-Pairing Complex of Lysozyme with Sodium Dodecyl Sulphate to Enhance Stability in Lipid-Based Carriers JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 16 PY - 2024 IS - 5 SP - 589 SN - 1999-4923 DO - 10.3390/pharmaceutics16050589 UR - https://m2.mtmt.hu/api/publication/34832111 ID - 34832111 AB - Hydrophobic ion pairing (HIP) complexation was found to be an efficient approach in modulating the release and enhancing the stability and encapsulation of hydrophilic macromolecules such as proteins in hydrophobic nano/microcarriers. The present work strives to develop and optimize the preparation of the HIP complex of the antimicrobial enzyme lysozyme (LYZ) with the ion-pairing agent (IPA) sodium dodecyl sulphate (SDS) relying on the quality-by-design (QbD) approach. The quality target product profile (QTPP) includes the achievement of maximal lipophilicity in a reversible manner to enable the maintenance of biological activity. The related critical quality attributes (CQAs) were defined as complexation efficacy, complex stability, enzyme recovery and activity. Three risk assessment (RA) tools were used to identify and rank the critical process parameters (CPPs) and critical material attributes (CMAs). From this assessment, the pH of the medium, LYZ:SDS molar ratio and drying conditions were determined as high-risk factors that need to be investigated. To the best of our knowledge, for the first time, electrostatic titration was used as a smart approach to determine the optimum molar ratio at different pH values. Based on the predefined CQAs, pH 8 with an LYZ/SDS molar ratio of 1:8 was found to be the optimal condition for complexation efficiency and recovery (%) of a biologically active enzyme. A cost-effective drying process based on a ventilated oven was developed, which resulted in complex qualities comparable to those obtained by the commonly used freeze-drying method. In a nutshell, the optimum conditions for the preparation of the LYZ/SDS HIP complex were efficiently facilitated by the rational application of QbD principles and the utilization of efficient electrostatic titration and ventilated oven-drying methods. LA - English DB - MTMT ER - TY - JOUR AU - Party, Petra AU - Sümegi, Sándor Soma AU - Ambrus, Rita TI - Preparation and Investigation of a Nanosized Piroxicam Containing Orodispersible Lyophilizate JF - MICROMACHINES J2 - MICROMACHINES-BASEL VL - 15 PY - 2024 IS - 4 SP - 532 SN - 2072-666X DO - 10.3390/mi15040532 UR - https://m2.mtmt.hu/api/publication/34830531 ID - 34830531 AB - Non-steroidal anti-inflammatory piroxicam (PRX) is a poorly water-soluble drug that provides relief in different arthritides. Reducing the particle size of PRX increases its bioavailability. For pediatric, geriatric, and dysphagic patients, oral dispersible systems ease administration. Moreover, fast disintegration followed by drug release and absorption through the oral mucosa can induce rapid systemic effects. We aimed to produce an orodispersible lyophilizate (OL) consisting of nanosized PRX. PRX was solved in ethyl acetate and then sonicated into a poloxamer-188 solution to perform spray-ultrasound-assisted solvent diffusion-based nanoprecipitation. The solid form was formulated via freeze drying in blister sockets. Mannitol and sodium alginate were applied as excipients. Dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) were used to determine the particle size. The morphology was characterized by scanning electron microscopy (SEM). To establish the crystallinity, X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) were used. A disintegration and in vitro dissolution test were performed. DLS and NTA presented a nanosized PRX diameter. The SEM pictures showed a porous structure. PRX became amorphous according to the XRPD and DSC curves. The disintegration time was less than 1 min and the dissolution profile improved. The final product was an innovative anti-inflammatory drug delivery system. LA - English DB - MTMT ER - TY - JOUR AU - Mardikasari, Sandra Aulia AU - Katona, Gábor AU - Sipos, Bence AU - Pannonhalminé Csóka, Ildikó TI - Essential considerations towards development of effective nasal antibiotic formulation: features, strategies, and future directions JF - EXPERT OPINION ON DRUG DELIVERY J2 - EXPERT OPIN DRUG DELIV PY - 2024 SP - 1 EP - 15 PG - 15 SN - 1742-5247 DO - 10.1080/17425247.2024.2341184 UR - https://m2.mtmt.hu/api/publication/34791444 ID - 34791444 LA - English DB - MTMT ER - TY - CONF AU - Mohammad, Hanan AU - Katona, Gábor AU - Pannonhalminé Csóka, Ildikó TI - Development of functionalized protein-based nanoparticles for improved drug delivery of antineurodegenerative drugs T2 - VI. Symposium of Young Researchers on PharmaceuticalTechnology, Biotechnology and Regulatory Science PB - Institute of Pharmaceutical Technology and RegulatoryAffairs, University of Szeged, Faculty of Pharmacy C1 - Szeged PY - 2024 SP - 61 EP - 61 PG - 1 DO - 10.14232/syrptbrs.2024.61 UR - https://m2.mtmt.hu/api/publication/34770849 ID - 34770849 LA - English DB - MTMT ER - TY - CONF AU - Salamah, Maryana AU - Balogh, György Tibor AU - Katona, Gábor TI - Formulation and optimization of rifampicin-loaded niosomes for ocular delivery system T2 - VI. Symposium of Young Researchers on PharmaceuticalTechnology, Biotechnology and Regulatory Science PB - Institute of Pharmaceutical Technology and RegulatoryAffairs, University of Szeged, Faculty of Pharmacy C1 - Szeged PY - 2024 SP - 48 EP - 48 PG - 1 DO - 10.14232/syrptbrs.2024.48 UR - https://m2.mtmt.hu/api/publication/34770843 ID - 34770843 LA - English DB - MTMT ER - TY - CONF AU - Cseh, Martin AU - Katona, Gábor AU - Berkó, Szilvia AU - Pannonhalminé Csóka, Ildikó TI - Design, development and investigation of dexamethasone sodium phosphate-containing dissolving microneedle arrays T2 - VI. Symposium of Young Researchers on PharmaceuticalTechnology, Biotechnology and Regulatory Science PB - Institute of Pharmaceutical Technology and RegulatoryAffairs, University of Szeged, Faculty of Pharmacy C1 - Szeged PY - 2024 SP - 24 EP - 24 PG - 1 DO - 10.14232/syrptbrs.2024.24 UR - https://m2.mtmt.hu/api/publication/34770823 ID - 34770823 LA - English DB - MTMT ER - TY - CONF AU - Mardikasari, Sandra Aulia AU - Katona, Gábor AU - Budai-Szűcs, Mária AU - Sipos, Bence AU - Orosz, László AU - Burián, Katalin AU - Rovó, László AU - Pannonhalminé Csóka, Ildikó TI - Development of in situ ionic-sensitive nasal gels of amoxicillin-loaded albumin nanoparticle applying QbD-based optimization for improved local delivery T2 - VI. Symposium of Young Researchers on PharmaceuticalTechnology, Biotechnology and Regulatory Science PB - Institute of Pharmaceutical Technology and RegulatoryAffairs, University of Szeged, Faculty of Pharmacy C1 - Szeged PY - 2024 SP - 18 EP - 18 PG - 1 DO - 10.14232/syrptbrs.2024.18 UR - https://m2.mtmt.hu/api/publication/34770795 ID - 34770795 LA - English DB - MTMT ER - TY - CONF AU - Rajab, Fatima AU - Sipos, Bence AU - Pannonhalminé Csóka, Ildikó TI - Nasal polymeric nanoparticles for enhanced therapeutic effects T2 - VI. Symposium of Young Researchers on PharmaceuticalTechnology, Biotechnology and Regulatory Science PB - Institute of Pharmaceutical Technology and RegulatoryAffairs, University of Szeged, Faculty of Pharmacy C1 - Szeged PY - 2024 SP - 50 EP - 50 PG - 1 DO - 10.14232/syrptbrs.2024.50 UR - https://m2.mtmt.hu/api/publication/34762705 ID - 34762705 LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Barbara AU - Horváth, Attila AU - Jójártné Laczkovich, Orsolya AU - Biró, Dalma AU - Matuz, Mária AU - Csupor, Dezső TI - Storage Conditions Influence the Quality of Ginger – A Stability Study Inspired by Clinical Trials JF - PLANTA MEDICA: NATURAL PRODUCTS AND MEDICINAL PLANT RESEARCH J2 - PLANTA MED PY - 2024 SN - 0032-0943 DO - 10.1055/a-2283-8147 UR - https://m2.mtmt.hu/api/publication/34728728 ID - 34728728 N1 - Funding Agency and Grant Number: Ministry of Innovation and Technology of Hungary [TKP2021-EGA, TKP2021-EGA-32]; Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund Funding text: This work was supported by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021-EGA funding scheme; project No. TKP2021-EGA-32 has been implemented with the support provided. AB - Ginger has traditionally been used to treat and prevent nausea and vomiting; however, the results of clinical trials are ambiguous. The efficacy of ginger is attributed to gingerols and their metabolites, shogaols. Since these compounds have different pharmacological profiles, the clinical efficacy of ginger products is largely dependent on their chemical composition. The goal of our study was to examine the stability of ginger determining the 6-gingerol contents in order to assess the effects of different storage conditions. We have performed a 6-month stability test with dry ginger rhizome samples stored in a constant climate chamber in three different storage containers (uncovered glass container, glass container sealed with rubber stopper, plastic container). 6-gingerol contents were measured by HPLC method. The concentration of 6-gingerol decreased in all samples. In the sealed glass container, the decrease of 6-gingerol content was significantly lower than in the unsealed glass container and in the plastic container. These results demonstrate that storage conditions have a significant impact on the quality of ginger, which may also affect efficacy. LA - English DB - MTMT ER - TY - CHAP AU - Ashfaq, Rabia AU - Budai-Szűcs, Mária ED - Varga, Patrícia Orsolya ED - Szalai, Boglárka ED - Uhljar, Luca Éva TI - Factorial design of injectable chlorhexidine in situ gelling system for the treatment of periodontitis T2 - VI. Symposium of Young Researchers on Pharmaceutical Technology, Biotechnology and Regulatory Science PB - Institute of Pharmaceutical Technology and Regulatory Affairs, University of Szeged, Faculty of Pharmacy CY - Szeged PY - 2024 SP - 19 EP - 19 PG - 1 DO - 10.14232/syrptbrs.2024.19 UR - https://m2.mtmt.hu/api/publication/34723711 ID - 34723711 LA - English DB - MTMT ER -