TY - JOUR AU - Horváth, Tamara AU - Sándor, Lilla AU - Baráth, Bálint AU - Donka, Tibor AU - Baráth, Bence AU - Mohácsi, Árpád AU - Jász, Dávid Kurszán AU - Hartmann, Petra AU - Boros, Mihály TI - Methane Admixture Protects Liver Mitochondria and Improves Graft Function after Static Cold Storage and Reperfusion JF - ANTIOXIDANTS J2 - ANTIOXIDANTS-BASEL VL - 12 PY - 2023 IS - 2 PG - 15 SN - 2076-3921 DO - 10.3390/antiox12020271 UR - https://m2.mtmt.hu/api/publication/33603762 ID - 33603762 AB - Mitochondria are targets of cold ischemia-reperfusion (IR), the major cause of cell damage during static cold preservation of liver allografts. The bioactivity of methane (CH4) has recently been recognized in various hypoxic and IR conditions as having influence on many aspects of mitochondrial biology. We therefore hypothesized that cold storage of liver grafts in CH4-enriched preservation solution can provide an increased defence against organ dysfunction in a preclinical rat model of liver transplantation. Livers were preserved for 24 h in cold histidine–tryptophan–ketoglutarate (HTK) or CH4-enriched HTK solution (HTK-CH4) (n = 24 each); then, viability parameters were monitored for 60 min during normothermic isolated reperfusion and perfusate and liver tissue were collected. The oxidative phosphorylation capacity and extramitochondrial Ca2+ movement were measured by high resolution respirometry. Oxygen and glucose consumption increased significantly while hepatocellular damage was decreased in the HTK-CH4 grafts compared to the HTK group. Mitochondrial oxidative phosphorylation capacity was more preserved (128.8 ± 31.5 pmol/s/mL vs 201.3 ± 54.8 pmol/s/mL) and a significantly higher Ca2+ flux was detected in HTK-CH4 storage (2.9 ± 0.1 mV/s) compared to HTK (2.3 ± 0.09 mV/s). These results demonstrate the direct effect of CH4 on hepatic mitochondrial function and extramitochondrial Ca2+ fluxes, which may have contributed to improved graft functions and a preserved histomorphology after cold IR. LA - English DB - MTMT ER - TY - CONF AU - Bozóki, Zoltán AU - Horváth, László AU - Huszár, Helga AU - Nagy, Zoltán AU - Pintér, Krisztina AU - Torma, Péter AU - Weidinger, Tamás TI - Nyitott fotoakusztikus kamra alkalmazása vízgőzfluxus mérésére T2 - ÉLETTERÜNK A LÉGKÖR. PY - 2022 SP - 5 UR - https://m2.mtmt.hu/api/publication/33298194 ID - 33298194 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Homik, Zsolt AU - Heszlerné Kopniczky, Judit AU - Smausz Kolumbán, Tamás AU - Berkesi, Dániel Simon AU - Hopp, Béla TI - Formation of gold/silver composite nanoparticles by pulsed laser ablation of gold–silver layered films in liquid JF - APPLIED PHYSICS A - MATERIALS SCIENCE AND PROCESSING J2 - APPL PHYS A-MATER VL - 128 PY - 2022 IS - 9 PG - 13 SN - 0947-8396 DO - 10.1007/s00339-022-05938-7 UR - https://m2.mtmt.hu/api/publication/33134651 ID - 33134651 AB - Nanoparticles of high purity can be produced from a variety of materials by pulsed laser ablation of solids in liquid. Composite nanoparticles are of great importance in various applications such as catalysis or biomedicine and the process of their formation is still a subject of intense research. In this work, gold/silver composite nanoparticles were synthesized in aqueous media by ns pulsed laser ablation of gold–silver multilayer targets with different absolute layer thicknesses and layer thickness ratios. The generated nanoparticles showed a log-normal distribution of sizes, with average diameter in the 20–40 nm range and standard deviation of 9–30 nm. By comparing the UV–VIS absorbance spectra of the nanoparticle colloids with two theoretical calculations (based on the Mie and the BEM model), it was found that there is a direct correlation between the average Au and Ag content of the nanoparticles and the composition of the films on the substrate. Assuming thermal ablation, our model calculations showed that there is a maximum thickness of the top layer up to which both layers can be ablated simultaneously and alloy nanoparticles can be produced. LA - English DB - MTMT ER - TY - JOUR AU - Huszár, Helga AU - Végh, Panna TI - Hűtőközeg-szivárgási pontok meghatározására alkalmas fotoakusztikus műszer fejlesztése JF - ÉPÜLETGÉPÉSZ: A MAGYAR ÉPŰLETGÉPÉSZEK SZÖVETSÉGÉNEK SZAKLAPJA J2 - ÉPÜLETGÉPÉSZ VL - 11 PY - 2022 IS - 1 SP - 44 EP - 46 PG - 3 SN - 2063-5400 UR - https://m2.mtmt.hu/api/publication/32856164 ID - 32856164 LA - Hungarian DB - MTMT ER - TY - GEN AU - Végh, Panna AU - Gábor, Gulyás AU - Huszár, Helga AU - Szabó, Gábor AU - Bozóki, Zoltán TI - Dependence of the photoacoustic sensitivity parameter on the heat capacity ratio of the measured gas and on the acoustic properties of the measuring system PY - 2021 SP - 313 UR - https://m2.mtmt.hu/api/publication/32856301 ID - 32856301 LA - English DB - MTMT ER - TY - JOUR AU - Jász, Dávid Kurszán AU - Szilágyi, Ágnes AU - Tuboly, Eszter AU - Baráth, Bálint AU - Márton, Anett Roxána AU - Varga, Petra AU - Varga, Gabriella AU - Érces, Dániel AU - Mohácsi, Árpád AU - Szabó, Anna AU - Bozó, Renáta AU - Gömöri, Kamilla AU - Görbe, Anikó AU - Boros, Mihály AU - Hartmann, Petra TI - Reduction in hypoxia-reoxygenation-induced myocardial mitochondrial damage with exogenous methane JF - JOURNAL OF CELLULAR AND MOLECULAR MEDICINE J2 - J CELL MOL MED VL - 25 PY - 2021 IS - 11 SP - 5113 EP - 5123 PG - 11 SN - 1582-1838 DO - 10.1111/jcmm.16498 UR - https://m2.mtmt.hu/api/publication/32012606 ID - 32012606 N1 - Institute of Surgical Research, University of Szeged, Szeged, Hungary MTA–SZTE Research Group on Photoacoustic Spectroscopy, University of Szeged, Szeged, Hungary Department of Dermatology and Allergology, University of Szeged, Szeged, Hungary Department of Biochemistry, University of Szeged, Szeged, Hungary Export Date: 29 September 2021 Correspondence Address: Hartmann, P.; Institute of Surgical Research, Hungary; email: hartmann.petra@med.u-szeged.hu Chemicals/CAS: cytochrome c, 9007-43-6, 9064-84-0; cytochrome c oxidase, 72841-18-0, 9001-16-5; hydrogen peroxide, 7722-84-1; methane, 74-82-8; pentobarbital, 57-33-0, 76-74-4; succinate dehydrogenase (ubiquinone), 9028-11-9 Tradenames: AxioImager, Zeiss, United States Manufacturers: Oroboros Instrument, Austria; Fluostar Optima, Germany; Hoffmann La Roche, Germany; Sigma, Hungary; Zeiss, United States Funding details: EFOP‐3.6.2‐16‐2017‐0006, GINOP‐2.3.2‐15‐2016‐00015 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH, NKFI K120232, NKFIH‐1279‐2/2020 Funding text 1: The study was funded by National Research Development and Innovation Office grant NKFI K120232 and NKFIH‐1279‐2/2020. It was further supported by the Economic Development and Innovation Operative Programme Grant (GINOP‐2.3.2‐15‐2016‐00015), Human Resource Development Operational Programme (EFOP‐3.6.2‐16‐2017‐0006). AB - Albeit previous experiments suggest potential anti-inflammatory effect of exogenous methane (CH4) in various organs, the mechanism of its bioactivity is not entirely understood. We aimed to investigate the potential mitochondrial effects and the underlying mechanisms of CH4 in rat cardiomyocytes and mitochondria under simulated ischaemia/reperfusion (sI/R) conditions. Three-day-old cultured cardiomyocytes were treated with 2.2% CH4-artificial air mixture during 2-hour-long reoxygenation following 4-hour-long anoxia (sI/R and sI/R + CH4, n = 6-6), with normoxic groups serving as controls (SH and SH + CH4; n = 6-6). Mitochondrial functions were investigated with high-resolution respirometry, and mitochondrial membrane injury was detected by cytochrome c release and apoptotic characteristics by using TUNEL staining. CH4 admixture had no effect on complex II (CII)-linked respiration under normoxia but significantly decreased the complex I (CI)-linked oxygen consumption. Nevertheless, addition of CH4 in the sI/R + CH4 group significantly reduced the respiratory activity of CII in contrast to CI and the CH4 treatment diminished mitochondrial H2O2 production. Substrate-induced changes to membrane potential were partially preserved by CH4, and additionally, cytochrome c release and apoptosis of cardiomyocytes were reduced in the CH4-treated group. In conclusion, the addition of CH4 decreases mitochondrial ROS generation via blockade of electron transport at CI and reduces anoxia-reoxygenation-induced mitochondrial dysfunction and cardiomyocyte injury in vitro. LA - English DB - MTMT ER - TY - JOUR AU - Benke, Kálmán AU - Jász, Dávid Kurszán AU - Szilágyi, Ágnes AU - Baráth, Bálint AU - Tuboly, Eszter AU - Márton, Anett Roxána AU - Varga, Petra AU - Mohácsi, Árpád AU - Szabó, Anna AU - Széll, Zsófia AU - Ruppert, Mihály AU - Radovits, Tamás AU - Szabó, Gábor AU - Merkely, Béla Péter AU - Hartmann, Petra AU - Boros, Mihály TI - Methane supplementation improves graft function in experimental heart transplantation JF - JOURNAL OF HEART AND LUNG TRANSPLANTATION J2 - J HEART LUNG TRANSPL VL - 40 PY - 2021 IS - 3 SP - 183 EP - 192 PG - 10 SN - 1053-2498 DO - 10.1016/j.healun.2020.11.003 UR - https://m2.mtmt.hu/api/publication/31662130 ID - 31662130 N1 - Heart and Vascular Centre, Semmelweis University, Budapest, Hungary Department of Cardiac Surgery, University of Halle, Halle, Germany Institute of Surgical Research, University of Szeged, Szeged, Hungary MTA–SZTE Research Group on Photoacoustic Spectroscopy, University of Szeged, Szeged, Hungary MTA–SZTE Research Group on Photoacoustic Spectroscopy, University of Szeged, Szeged, Hungary Cited By :2 Export Date: 23 January 2023 CODEN: JHLTE Correspondence Address: Hartmann, P.; Institute of Surgical Research, H-6724 Szeged Pulz u. 1., Hungary AB - BACKGROUND: Maintenance of cell viability during cold storage is a key issue in organ transplantation. Methane (CH4) bioactivity has recently been recognized in ischemia/reperfusion conditions; we therefore hypothesized that cold storage in CH4-enriched preservation solution can provide an increased defense against organ dysfunction during experimental heart transplantation (HTX). METHODS: The hearts of donor Lewis rats were stored for 60 minutes in cold histidine-tryptophan-ketoglutarate (Custodiol [CS]) or CH4-saturated CS solution (CS-CH4) (n = 12 each). Standard heterotopic HTX was performed, and 60 minutes later, the left ventricular (LV) pressure-volume relationships LV systolic pressure (LVSP), systolic pressure increment (dP/dtmax), diastolic pressure decrement, and coronary blood flow (CBF) were measured. Tissue samples were taken to detect proinflammatory parameters, structural damage (by light microscopy), endoplasmic reticulum (ER) stress, and apoptosis markers (CCAAT/enhancer binding protein [C/EBP] homologous protein, GRP78, glycogen synthase kinase-3 beta, very low-density lipoprotein receptor, caspase 3 and 9, B-cell lymphoma 2, and bcl-2-like protein 4), whereas mitochondrial functional changes were analyzed by high-resolution respirometry. RESULTS: LVSP and dP/dtmax increased significantly at the largest pre-load volumes in CS-CH4 grafts as compared with the CS group (114.5 +/- 16.6 mm Hg vs 82.8 +/- 4.6 mm Hg and 3,133 +/- 430 mm Hg/s vs 1,739 +/- 169 mm Hg/s, respectively); the diastolic function and CBF (2.4 +/- 0.4 ml/min/g vs 1.3 +/- 0.3 ml/min/g) also improved. Mitochondrial oxidative phosphorylation capacity was more preserved (58.5 +/- 9.4 pmol/s/ml vs 27.7 +/- 6.6 pmol/s/ml), and cytochrome c release was reduced in CS-CH4 storage. Signs of HTX-caused myocardial damage, level of ER stress, and the transcription of proapoptotic proteins were significantly lower in CS-CH4 grafts. CONCLUSION: The addition of CH4 during 1 hour of cold storage improved early in vitro graft function and reduced mitochondrial dysfunction and activation of inflammation. Evidence shows that CH4 reduced ER stress-linked proapoptotic signaling. (C) 2020 International Society for Heart and Lung Transplantation. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Bársony, Anett AU - Noémi, Vida AU - Ámos, Gajda AU - Rutai, Attila AU - Mohácsi, Árpád AU - Szabó, Anna AU - Boros, Mihály AU - Varga, Gabriella AU - Érces, Dániel TI - Methane Exhalation Can Monitor the Microcirculatory Changes of the Intestinal Mucosa in a Large Animal Model of Hemorrhage and Fluid Resuscitation JF - FRONTIERS IN MEDICINE J2 - FRONT MED VL - 7 PY - 2020 PG - 11 SN - 2296-858X DO - 10.3389/fmed.2020.567260 UR - https://m2.mtmt.hu/api/publication/31637828 ID - 31637828 N1 - Department of Surgery, University of Szeged, Szeged, Hungary Institute of Surgical Research, University of Szeged, Szeged, Hungary MTA–SZTE Research Group on Photoacoustic Spectroscopy, Szeged, Hungary Department of Optics and Quantum Electronics, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary Export Date: 29 September 2021 Correspondence Address: Érces, D.; Institute of Surgical Research, Hungary; email: erces.daniel@med.u-szeged.hu Chemicals/CAS: hetastarch, 9005-27-0; methane, 74-82-8 Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH, EFOP-3.6.2-16-2017-00006, GINOP-2.3.2-15-2016-00015, NKFIH-K116861, NKFIH-K120232, TUDFO/47138-1/2019-ITM Funding text 1: The authors are grateful to Andrea B?s, Csilla Mester, Nikolett Beretka, Bence Gyorfi, and P?ter S?rk?ny for their skillful assistance. Funding. This study was supported by the Hungarian National Research, Development and Innovation Office grants NKFIH-K120232 and NKFIH-K116861 as well as GINOP-2.3.2-15-2016-00015 and EFOP-3.6.2-16-2017-00006. University of Szeged Open Access Fund 4749 and FIKP programme grant (TUDFO/47138-1/2019-ITM). LA - English DB - MTMT ER - TY - JOUR AU - Varga, Petra AU - Vida, Noémi AU - Hartmann, Petra AU - Szabó, Anna AU - Mohácsi, Árpád AU - Szabó, Gábor AU - Boros, Mihály AU - Tuboly, Eszter TI - Alternative methanogenesis - Methanogenic potential of organosulfur administration JF - PLOS ONE J2 - PLOS ONE VL - 15 PY - 2020 IS - 7 PG - 12 SN - 1932-6203 DO - 10.1371/journal.pone.0236578 UR - https://m2.mtmt.hu/api/publication/31395807 ID - 31395807 N1 - Institute of Surgical Research, University of Szeged, Szeged, Hungary MTA-SZTE Research Group on Photoacoustic Spectroscopy, University of Szeged, Szeged, Hungary Department of Pharmacology and Therapeutics, University College Cork (UCC), Cork, Ireland Export Date: 7 April 2022 CODEN: POLNC Correspondence Address: Tuboly, E.; Department of Pharmacology and Therapeutics, Ireland; email: eszter.tuboly@ucc.ie LA - English DB - MTMT ER - TY - JOUR AU - Szitás, Ádám AU - Gubó, Richard AU - Pásztor, Tibor AU - Farkas, Arnold Péter AU - Ajtai, Tibor AU - Óvári, László AU - Palotás, Krisztián AU - Berkó, András AU - Kónya, Zoltán TI - Adsorption of Azobenzene on Hexagonal Boron Nitride Nanomesh Supported by Rh(111) JF - JOURNAL OF PHYSICAL CHEMISTRY C J2 - J PHYS CHEM C VL - 124 PY - 2020 IS - 26 SP - 14182 EP - 14194 PG - 13 SN - 1932-7447 DO - 10.1021/acs.jpcc.0c01725 UR - https://m2.mtmt.hu/api/publication/31388984 ID - 31388984 LA - English DB - MTMT ER -