TY - JOUR AU - Masood, Thann Oon Saleh Saeed Saleh AU - Lakatos, Szandra AU - Karcsúné Kis, Gyöngyi AU - Ignácz, Melissza AU - Domoki, Ferenc AU - Rosta, Judit TI - Subarachnoid Hemorrhage Depletes Calcitonin Gene-Related Peptide Levels of Trigeminal Neurons in Rat Dura Mater JF - CELLS J2 - CELLS-BASEL VL - 13 PY - 2024 IS - 8 PG - 18 SN - 2073-4409 DO - 10.3390/cells13080653 UR - https://m2.mtmt.hu/api/publication/34794768 ID - 34794768 AB - Subarachnoid hemorrhage (SAH) remains a major cause of cerebrovascular morbidity, eliciting severe headaches and vasospasms that have been shown to inversely correlate with vasodilator calcitonin gene-related peptide (CGRP) levels. Although dura mater trigeminal afferents are an important source of intracranial CGRP, little is known about the effects of SAH on these neurons in preclinical models. The present study evaluated changes in CGRP levels and expression in trigeminal primary afferents innervating the dura mater 72 h after experimentally induced SAH in adult rats. SAH, eliciting marked damage revealed by neurological examination, significantly reduced the density of CGRP-immunoreactive nerve fibers both in the dura mater and the trigeminal caudal nucleus in the medulla but did not affect the total dural nerve fiber density. SAH attenuated ex vivo dural CGRP release by ~40% and in the trigeminal ganglion, reduced both CGRP mRNA levels and the number of highly CGRP-immunoreactive cell bodies. In summary, we provide novel complementary evidence that SAH negatively affects the integrity of the CGRP-expressing rat trigeminal neurons. Reduced CGRP levels suggest likely impaired meningeal neurovascular functions contributing to SAH complications. Further studies are to be performed to reveal the importance of impaired CGRP synthesis and its consequences in central sensory processing. LA - English DB - MTMT ER - TY - JOUR AU - Lőrincz, Csanád Endre AU - Börzsei, Denise AU - Nagyné Hoffmann, Alexandra AU - Varga, Csaba AU - Szabó, Renáta TI - Mechanisms and Target Parameters in Relation to Polycystic Ovary Syndrome and Physical Exercise: Focus on the Master Triad of Hormonal Changes, Oxidative Stress, and Inflammation JF - BIOMEDICINES J2 - BIOMEDICINES VL - 12 PY - 2024 IS - 3 SP - 560 SN - 2227-9059 DO - 10.3390/biomedicines12030560 UR - https://m2.mtmt.hu/api/publication/34742326 ID - 34742326 AB - Polycystic ovary syndrome (PCOS) is a common endocrine disorder among females of reproductive age with heterogeneous prevalence. It is well known that female reproductive competence depends on the dynamic regulation of the hypothalamic–pituitary–gonadal (HPG) axis; therefore, disruption of this highly regulated system leads to fertility problems. Among disruptors, both oxidative stress and inflammation contribute to an increased LH-FSH ratio and a consequent hyperandrogenism. Shifts in this bidirectional interplay between the neuroendocrine system and oxidative/inflammatory homeostasis result in the accumulation of reactive oxygen/nitrogen species and inflammatory markers as well as alterations in antioxidant defense mechanisms. Evidence shows that lifestyle changes, including regular physical exercise, are recognized as the most effective first-line management to reduce the severity of PCOS symptoms. The aim of our narrative review is to provide insights into the mechanisms and target factors of PCOS-related hormonal changes, oxidative/antioxidant homeostasis, and inflammation, and to discuss the effects of exercise, which takes into account various factors, in relation to PCOS. A better understanding of the PCOS-associated hormonal changes, oxidative and inflammatory circuits, as well as exercise-induced mechanisms of action on those targets may improve the quality of life of women with PCOS. LA - English DB - MTMT ER - TY - CONF AU - Péter, Bátor Kemenesi-Gedei AU - Karcsúné Kis, Gyöngyi TI - Oxytocin Receptor Expression In Primary Sensory Neurons: Unveiling How Oxytocin And Its Receptor Contribute To The Sensory Processing And The Modulation Of Pain T2 - International Neuroscience Conference, Pécs 2024 PY - 2024 SP - S6.05 UR - https://m2.mtmt.hu/api/publication/34694071 ID - 34694071 N1 - (Poszter) LA - English DB - MTMT ER - TY - JOUR AU - Bónus, Lilla AU - Nagy Lászlóné Antal, Erzsébet AU - Korom, Erzsébet TI - Digital game-based inquiry learning to improve eighth graders’ inquiry skills in biology JF - JOURNAL OF SCIENCE EDUCATION AND TECHNOLOGY J2 - J SCI EDUC TECHNOL PY - 2024 SN - 1059-0145 DO - 10.1007/s10956-024-10096-x UR - https://m2.mtmt.hu/api/publication/34572141 ID - 34572141 N1 - Doctoral School of Education, University of Szeged, Petőfi sgt. 32–34, Szeged, H–6722, Hungary Institute of Education, University of Szeged, Petőfi sgt. 32–34, Szeged, H–6722, Hungary MTA–SZTE Science Education Research Group, University of Szeged, Petőfi sgt. 32–34, Szeged, H–6722, Hungary MTA–SZTE Digital Learning Technologies Research Group, University of Szeged, Petőfi sgt. 32–34, Szeged, H–6722, Hungary Biology Methodology Group, Department of Physiology Anatomy and Neuroscience, University of Szeged, Közép Fasor 52, Szeged, H–6726, Hungary Export Date: 13 April 2024 Correspondence Address: Korom, E.; Institute of Education, Petőfi sgt. 32–34, Hungary; email: korom@edpsy.u-szeged.hu LA - English DB - MTMT ER - TY - JOUR AU - Jójárt, Boldizsár AU - Csákány-Papp, Noémi AU - Laub, H AU - Vasas, Béla AU - Barta, B AU - Szabó, Viktória AU - Bagyánszki, Mária AU - Mándity, I AU - Heltovics, G AU - Pallagi, Petra AU - Maléth, József TI - P072 LDN-071, a novel first-in-class amino acid-based PAI-1 inhibitor, significantly reduces the severity of experimental colitis in mice JF - JOURNAL OF CROHNS & COLITIS J2 - J CROHNS COLITIS VL - 18 PY - 2024 IS - Supplement_1 SP - i342 EP - i342 PG - 1 SN - 1873-9946 DO - 10.1093/ecco-jcc/jjad212.0202 UR - https://m2.mtmt.hu/api/publication/34538600 ID - 34538600 LA - English DB - MTMT ER - TY - JOUR AU - El Heni, Heni AU - Kemenesi-Gedei, Péter Bátor AU - Pálvölgyi, Laura AU - Szeredi, Ivett Dorina AU - Karcsúné Kis, Gyöngyi TI - Peripheral Branch Injury Induces Oxytocin Receptor Expression at the Central Axon Terminals of Primary Sensory Neurons JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 1 PG - 15 SN - 1661-6596 DO - 10.3390/ijms25010007 UR - https://m2.mtmt.hu/api/publication/34443978 ID - 34443978 AB - Considerable evidence suggests that oxytocin, as a regulatory nonapeptide, participates in modulatory mechanisms of nociception. Nonetheless, the role of this hypothalamic hormone and its receptor in the sensory pathway has yet to be fully explored. The present study performed immunohistochemistry, enzyme-linked immunosorbent assay, and RT-qPCR analysis to assess changes in the expression of the neuronal oxytocin receptor in female rats following tight ligation of the sciatic nerve after 1, 3, and 7 days of survival. Oxytocin receptor immunoreactivity was present in both dorsal root ganglia and lumbar spinal cord segments, but not accumulated at the site of the ligation of the peripheral nerve branch. We found a time-dependent change in the expression of oxytocin receptor mRNA in L5 dorsal root ganglion neurons, as well as an increase in the level of the receptor protein in the lumbar segment of the spinal cord. A peak in the expression was observed on day 3, which downturned slightly by day 7 after the nerve ligation. These results show that OTR expression is up-regulated in response to peripheral nerve lesions. We assume that the importance of OTR is to modify spinal presynaptic inputs of the sensory neurons upon injury-induced activation, thus to be targets of the descending oxytocinergic neurons from supraspinal levels. The findings of this study support the concept that oxytocin plays a role in somatosensory transmission. LA - English DB - MTMT ER - TY - GEN AU - Szabó, Viktória AU - Csákány-Papp, Noémi AU - Görög, Marietta AU - Madácsy, Tamara AU - Varga, Árpád AU - Kiss, Aletta Kata AU - Tél, Bálint AU - Jójárt, Boldizsár AU - Crul, Tim AU - Dudás, Krisztina Márta AU - Bagyánszki, Mária AU - Bódi, Nikolett AU - Ayaydin, Ferhan AU - Shyam, Jee AU - Tiszlavicz, László AU - Stauderman, Kenneth AU - Hebbar, Sudarshan AU - Pallagi, Petra AU - Maléth, József TI - The inhibition of Orai1 calcium channel reduces the progression of chronic pancreatitis PY - 2023 SP - 1 UR - https://m2.mtmt.hu/api/publication/34672053 ID - 34672053 LA - English DB - MTMT ER - TY - JOUR AU - Jójárt, Boldizsár AU - Csákány-Papp, Noémi AU - H., Laub AU - Vasas, Béla AU - Barta, Bence Pál AU - Szabó, Viktória AU - Bagyánszki, Mária AU - I., Mándity AU - G., Heltovics AU - Pallagi, Petra AU - Maléth, József TI - LDN-071, a novel first-in-class peptide-based plasminogen activator inhibitor 1, significantly reduces the severity of experimental colitis in mic JF - UNITED EUROPEAN GASTROENTEROLOGY JOURNAL J2 - UEG JOURNAL VL - 11 PY - 2023 IS - S8 SP - 209 EP - 2010 PG - 1802 SN - 2050-6406 UR - https://m2.mtmt.hu/api/publication/34593826 ID - 34593826 LA - English DB - MTMT ER - TY - JOUR AU - Gémes, Nikolett AU - Balog, József Ágoston AU - Neuperger, Patricia AU - Schlegl, Erzsébet AU - Barta, Imre AU - Fillinger, János AU - Antus, Balázs AU - Zvara, Ágnes AU - Hegedűs, Zoltán AU - Czimmerer, Zsolt AU - Manczinger, Máté AU - Balogh, Gergő Mihály AU - Tóvári, József AU - Puskás, László AU - Szebeni, Gábor TI - Single-cell immunophenotyping revealed the association of CD4+ central and CD4+ effector memory T cells linking exacerbating chronic obstructive pulmonary disease and NSCLC. JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 14 PY - 2023 PG - 16 SN - 1664-3224 DO - 10.3389/fimmu.2023.1297577 UR - https://m2.mtmt.hu/api/publication/34486293 ID - 34486293 N1 - * Megosztott szerzőség AB - Tobacco smoking generates airway inflammation in chronic obstructive pulmonary disease (COPD), and its involvement in the development of lung cancer is still among the leading causes of early death. Therefore, we aimed to have a better understanding of the disbalance in immunoregulation in chronic inflammatory conditions in smoker subjects with stable COPD (stCOPD), exacerbating COPD (exCOPD), or non-small cell lung cancer (NSCLC).Smoker controls without chronic illness were recruited as controls. Through extensive mapping of single cells, surface receptor quantification was achieved by single-cell mass cytometry (CyTOF) with 29 antibodies. The CyTOF characterized 14 main immune subsets such as CD4+, CD8+, CD4+/CD8+, CD4-/CD8-, and γ/δ T cells and other subsets such as CD4+ or CD8+ NKT cells, NK cells, B cells, plasmablasts, monocytes, CD11cdim, mDCs, and pDCs. The CD4+ central memory (CM) T cells (CD4+/CD45RA-/CD45RO+/CD197+) and CD4+ effector memory (EM) T cells (CD4+/CD45RA-/CD45RO+/CD197-) were FACS-sorted for RNA-Seq analysis. Plasma samples were assayed by Luminex MAGPIX® for the quantitative measurement of 17 soluble immuno-oncology mediators (BTLA, CD28, CD80, CD27, CD40, CD86, CTLA-4, GITR, GITRL, HVEM, ICOS, LAG-3, PD-1, PD-L1, PD-L2, TIM-3, TLR-2) in the four studied groups.Our focus was on T-cell-dependent differences in COPD and NSCLC, where peripheral CD4+ central memory and CD4+ effector memory cells showed a significant reduction in exCOPD and CD4+ CM showed elevation in NSCLC. The transcriptome analysis delineated a perfect correlation of differentially expressed genes between exacerbating COPD and NSCLC-derived peripheral CD4+ CM or CD4+ EM cells. The measurement of 17 immuno-oncology soluble mediators revealed a disease-associated phenotype in the peripheral blood of stCOPD, exCOPD, and NSCLC patients.The applied single-cell mass cytometry, the whole transcriptome profiling of peripheral CD4+ memory cells, and the quantification of 17 plasma mediators provided complex data that may contribute to the understanding of the disbalance in immune homeostasis generated or sustained by tobacco smoking in COPD and NSCLC. LA - English DB - MTMT ER - TY - JOUR AU - Lee, B.R. AU - Dalley, R. AU - Miller, J.A. AU - Chartrand, T. AU - Close, J. AU - Mann, R. AU - Mukora, A. AU - Ng, L. AU - Alfiler, L. AU - Baker, K. AU - Bertagnolli, D. AU - Brouner, K. AU - Casper, T. AU - Csajbók, Éva AU - Donadio, N. AU - Driessens, S.L.W. AU - Egdorf, T. AU - Enstrom, R. AU - Galakhova, A.A. AU - Gary, A. AU - Gelfand, E. AU - Goldy, J. AU - Hadley, K. AU - Heistek, T.S. AU - Hill, D. AU - Hou, W.-H. AU - Johansen, N. AU - Jorstad, N. AU - Kim, L. AU - Kocsis, Ágnes Katalin AU - Kruse, L. AU - Kunst, M. AU - León, G. AU - Long, B. AU - Mallory, M. AU - Maxwell, M. AU - McGraw, M. AU - McMillen, D. AU - Melief, E.J. AU - Molnár, Gábor AU - Mortrud, M.T. AU - Newman, D. AU - Nyhus, J. AU - Opitz-Araya, X. AU - Ozsvár, A. AU - Pham, T. AU - Pom, A. AU - Potekhina, L. AU - Rajanbabu, R. AU - Ruiz, A. AU - Sunkin, S.M. AU - Szöts, Ildikó AU - Taskin, N. AU - Thyagarajan, B. AU - Tieu, M. AU - Trinh, J. AU - Vargas, S. AU - Vumbaco, D. AU - Waleboer, F. AU - Walling-Bell, S. AU - Weed, N. AU - Williams, G. AU - Wilson, J. AU - Yao, S. AU - Zhou, T. ED - Barzó, Pál / Collaborator AU - Bakken, T. AU - Cobbs, C. AU - Dee, N. AU - Ellenbogen, R.G. AU - Esposito, L. AU - Ferreira, M. AU - Gouwens, N.W. AU - Grannan, B. AU - Gwinn, R.P. AU - Hauptman, J.S. AU - Hodge, R. AU - Jarsky, T. AU - Keene, C.D. AU - Ko, A.L. AU - Korshoej, A.R. AU - Levi, B.P. AU - Meier, K. AU - Ojemann, J.G. AU - Patel, A. AU - Ruzevick, J. AU - Silbergeld, D.L. AU - Smith, K. AU - Sørensen, J.C. AU - Waters, J. AU - Zeng, H. AU - Berg, J. AU - Capogna, M. AU - Goriounova, N.A. AU - Kalmbach, B. AU - de, Kock C.P.J. AU - Mansvelder, H.D. AU - Sorensen, S.A. AU - Tamás, Gábor AU - Lein, E.S. AU - Ting, J.T. TI - Signature morphoelectric properties of diverse GABAergic interneurons in the human neocortex JF - SCIENCE J2 - SCIENCE VL - 382 PY - 2023 IS - 6667 PG - 16 SN - 0036-8075 DO - 10.1126/science.adf6484 UR - https://m2.mtmt.hu/api/publication/34289826 ID - 34289826 N1 - Allen Institute for Brain Science, Seattle, WA 98109, USA Allen Institute for Neural Dynamics, Seattle, WA 98109, USA MTA-SZTE Research Group for Cortical Microcircuits, Department of Physiology, Anatomy, Neuroscience, University of Szeged, Szeged, 6726, Hungary Department of Integrative Neurophysiology, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit, Amsterdam, 1081 HV, Netherlands Department of Biomedicine, Aarhus University, Denmark Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA Department of Neurosurgery, University of Szeged, Szeged, 6725, Hungary Swedish Neuroscience Institute, Seattle, WA 98122, USA Department of Neurological Surgery, University of Washington, Seattle, WA 98195, USA Department of Neurosurgery, Aarhus University Hospital, Denmark Department of Anesthesiology, Aarhus University Hospital, Denmark Center for Experimental Neuroscience, Aarhus University Hospital, Denmark Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA Export Date: 10 November 2023; Cited By: 0 LA - English DB - MTMT ER -