TY - JOUR AU - Rónavári, Andrea AU - Ochirkhuyag, Altantuya AU - Igaz, Nóra AU - Szerencsés, Bettina AU - Ballai, Gergő AU - Huliák, Ildikó AU - Bocz, Csenge AU - Kovács, Ákos AU - Pfeiffer, Ilona AU - Csontné Kiricsi, Mónika AU - Kónya, Zoltán TI - Preparation, characterization and in vitro evaluation of the antimicrobial and antitumor activity of MnOx nanoparticles JF - COLLOIDS AND SURFACES A : PHYSICOCHEMICAL AND ENGINEERING ASPECTS J2 - COLLOID SURFACE A VL - 688 PY - 2024 SN - 0927-7757 DO - 10.1016/j.colsurfa.2024.133528 UR - https://m2.mtmt.hu/api/publication/34687262 ID - 34687262 LA - English DB - MTMT ER - TY - JOUR AU - Gombás, Bence György AU - Villanyi, Zoltan TI - 1,6-Hexanediol Is Inducing Homologous Recombination by Releasing BLM from Assemblysomes in Drosophila melanogaster JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 3 PG - 12 SN - 1661-6596 DO - 10.3390/ijms25031611 UR - https://m2.mtmt.hu/api/publication/34540973 ID - 34540973 AB - We recently demonstrated that 1,6-hexanediol inhibits the formation of assemblysomes. These membraneless cell organelles have important roles in co-translational protein complex assembly and also store halfway translated DNA damage response proteins for a timely stress response. Recognizing the therapeutic potential of 1,6-hexanediol in dismantling assemblysomes likely to be involved in chemo- or radiotherapy resistance of tumor cells, we initiated an investigation into the properties of 1,6-hexanediol. Our particular interest was to determine if this compound induces DNA double-strand breaks by releasing the BLM helicase. Its yeast ortholog Sgs1 was confirmed to be a component of assemblysomes. The BLM helicase induces DNA damage when overexpressed due to the DNA double-strand breaks it generates during its normal function to repair DNA damage sites. It is evident that storing Sgs1 helicase in assemblysomes is crucial to express the full-length functional protein only in the event of DNA damage. Alternatively, if we dissolve assemblysomes using 1,6-hexanediol, ribosome-nascent chain complexes might become targets of ribosome quality control. We explored these possibilities and found, through the Drosophila wing-spot test assay, that 1,6-hexanediol induces DNA double-strand breaks. Lethality connected to recombination events following 1,6-hexanediol treatment can be mitigated by inducing DNA double-strand breaks with X-ray. Additionally, we confirmed that SMC5 recruits DmBLM to DNA damage sites, as knocking it down abolishes the rescue effect of DNA double-strand breaks on 1,6-hexanediol-induced lethality in Drosophila melanogaster. LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Magyar, Lilla Brigitta AU - Kovács, Henrietta AU - Varga, Viktória AU - Bodai, László AU - Zsindely, Nóra AU - Nagy, Gábor AU - Hegedűs, Zoltán AU - Hultmark, Dan AU - Andó, István TI - Distinctive features of Zaprionus indianus hemocyte differentiation and function revealed by transcriptomic analysis JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 14 PY - 2023 PG - 14 SN - 1664-3224 DO - 10.3389/fimmu.2023.1322381 UR - https://m2.mtmt.hu/api/publication/34446740 ID - 34446740 N1 - * Megosztott szerzőség LA - English DB - MTMT ER - TY - JOUR AU - Farkas, Anita AU - Zsindely, Nóra AU - Nagy, Gábor AU - Kovács, Levente AU - Deák, Péter AU - Bodai, László TI - The ubiquitin thioesterase YOD1 ameliorates mutant Huntingtin induced pathology in Drosophila JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 13 SN - 2045-2322 DO - 10.1038/s41598-023-49241-8 UR - https://m2.mtmt.hu/api/publication/34431874 ID - 34431874 AB - Huntington’s disease (HD) is a neurodegenerative disorder caused by a dominant gain-of-function mutation in the huntingtin gene, resulting in an elongated polyglutamine repeat in the mutant Huntingtin (mHtt) that mediates aberrant protein interactions. Previous studies implicated the ubiquitin–proteasome system in HD, suggesting that restoring cellular proteostasis might be a key element in suppressing pathology. We applied genetic interaction tests in a Drosophila model to ask whether modulating the levels of deubiquitinase enzymes affect HD pathology. By testing 32 deubiquitinase genes we found that overexpression of Yod1 ameliorated all analyzed phenotypes, including neurodegeneration, motor activity, viability, and longevity. Yod1 did not have a similar effect in amyloid beta overexpressing flies, suggesting that the observed effects might be specific to mHtt. Yod1 overexpression did not alter the number of mHtt aggregates but moderately increased the ratio of larger aggregates. Transcriptome analysis showed that Yod1 suppressed the transcriptional effects of mHtt and restored the expression of genes involved in neuronal plasticity, vesicular transport, antimicrobial defense, and protein synthesis, modifications, and clearance. Furthermore, Yod1 overexpression in HD flies leads to the upregulation of genes involved in transcriptional regulation and synaptic transmission, which might be part of a response mechanism to mHtt-induced stress. LA - English DB - MTMT ER - TY - JOUR AU - Vörös, András AU - Nagy-Mikó, Bence AU - Oláh, Orsolya AU - Pankotai, Tibor AU - Újfaludi, Zsuzsanna AU - Nikolényi, Alíz AU - Lázár, György ifj AU - Ormandi, K AU - Villanyi, Zoltan TI - Cytoplasmic aggregation of RPB1 predicts failure of neoadjuvant chemotherapy against invasive carcinoma of no special type JF - VIRCHOWS ARCHIV J2 - VIRCHOWS ARCH VL - 483 PY - 2023 IS - S1 SP - S170 EP - S171 PG - 2 SN - 0945-6317 UR - https://m2.mtmt.hu/api/publication/34410430 ID - 34410430 LA - English DB - MTMT ER - TY - JOUR AU - Bohács, István AU - Erdenebileg, Khajidmaa AU - Kotormán, Márta TI - Investigation of the anti-amyloidogenic effect of quercetin and choline bitartrate JF - CURRENT TOPICS IN PEPTIDE AND PROTEIN RESEARCH J2 - CURR TOP PEPT PROT RES VL - 24 PY - 2023 SP - 11 EP - 16 PG - 6 SN - 0972-4524 UR - https://m2.mtmt.hu/api/publication/34409719 ID - 34409719 LA - English DB - MTMT ER - TY - JOUR AU - Datki, Zsolt László AU - Darula, Zsuzsanna AU - Vedelek, Viktor AU - Hunyadi-Gulyás Éva, Csilla AU - Dingmann, Brian J. AU - Vedelek, Balázs AU - Kalman, Janos AU - Urban, Peter AU - Gyenesei, Attila AU - Galik-Olah, Zita AU - Gálik, Bence AU - Sinka, Rita TI - Biofilm formation initiating rotifer-specific biopolymer and its predicted components JF - INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES J2 - INT J BIOL MACROMOL VL - 253 PY - 2023 IS - Part 5 PG - 14 SN - 0141-8130 DO - 10.1016/j.ijbiomac.2023.127157 UR - https://m2.mtmt.hu/api/publication/34334193 ID - 34334193 N1 - Megosztott szerzőség AB - The rotifer-specific biopolymer, namely Rotimer, is a recently discovered group of the biomolecule family. Rotimer has an active role in the biofilm formation initiated by rotifers (e.g., Euchlanis dilatata or Adineta vaga) or in the female-male sexual interaction of monogononts. To understand the Ca2+- and polarity-dependent formation of this multifunctional viscoelastic material, it is essential to explore its molecular composition. The investigation of the rotifer-enhanced biofilm and Rotimer-inductor conglomerate (RIC) formation yielded several protein candidates to predict the Rotimer-specific main components. The exudate of E. dilatata males was primarily applied from different biopolimer-containing samples (biofilm or RIC). The advantage of males over females lies in their degenerated digestive system and simple anatomy. Thus, their exudate is less contaminated with food and endosymbiont elements. The sequenced and annotated genome and transcriptome of this species opened the way for identifying Rotimer proteins by mass spectrometry. The predicted rotifer-biopolymer forming components are SCO-spondins and 14-3-3 protein. The characteristics of Rotimer are similar to Reissner's fiber, which is found in the central nervous system of vertebrates and is mainly formed from SCO-spondins. This molecular information serves as a starting point for its interdisciplinary investigation and application in biotechnology, biomedicine, or neurodegeneration-related drug development. LA - English DB - MTMT ER - TY - JOUR AU - Nagy-Mikó, Bence AU - Szatmári, Orsolya AU - Faragó-Mészáros, Réka AU - Csókási, Aliz AU - Bognár, Bence AU - Ördög, Nóra AU - Borsos, Barbara Nikolett AU - Majoros, Hajnalka AU - Újfaludi, Zsuzsanna AU - Oláh, Orsolya AU - Nikolényi, Alíz AU - Dobi, Ágnes AU - Kószó, Renáta Lilla AU - Sántha, Dóra AU - Lázár, György ifj AU - Simonka, Zsolt AU - Paszt, Attila AU - Ormándi, Katalin AU - Pankotai, Tibor AU - Boros, Imre Miklós AU - Villanyi, Zoltan AU - Vörös, András TI - Predictive Potential of RNA Polymerase B (II) Subunit 1 (RPB1) Cytoplasmic Aggregation for Neoadjuvant Chemotherapy Failure JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 21 PG - 9 SN - 1661-6596 DO - 10.3390/ijms242115869 UR - https://m2.mtmt.hu/api/publication/34230980 ID - 34230980 AB - We aimed to investigate the contribution of co-translational protein aggregation to the chemotherapy resistance of tumor cells. Increased co-translational protein aggregation reflects altered translation regulation that may have the potential to buffer transcription under genotoxic stress. As an indicator for such an event, we followed the cytoplasmic aggregation of RPB1, the aggregation-prone largest subunit of RNA polymerase II, in biopsy samples taken from patients with invasive carcinoma of no special type. RPB1 frequently aggregates co-translationally in the absence of proper HSP90 chaperone function or in ribosome mutant cells as revealed formerly in yeast. We found that cytoplasmic foci of RPB1 occur in larger sizes in tumors that showed no regression after therapy. Based on these results, we propose that monitoring the cytoplasmic aggregation of RPB1 may be suitable for determining—from biopsy samples taken before treatment—the effectiveness of neoadjuvant chemotherapy. LA - English DB - MTMT ER - TY - BOOK ED - Kónya, Zoltán ED - Csontné Kiricsi, Mónika TI - A Magyar Tudományos Akadémia Analítikai és Környezetkémiai Bizottságának 11. Környezetkémiai Szimpóziuma. (2023) PY - 2023 SP - 19 SN - 9789633069547 UR - https://m2.mtmt.hu/api/publication/34205216 ID - 34205216 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Zsindely, Nóra AU - Nagy, Gábor AU - Siági, Fruzsina AU - Farkas, Anita AU - Bodai, László TI - Dysregulated miRNA and mRNA Expression Affect Overlapping Pathways in a Huntington’s Disease Model JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 15 PG - 21 SN - 1661-6596 DO - 10.3390/ijms241511942 UR - https://m2.mtmt.hu/api/publication/34173172 ID - 34173172 AB - Huntington’s disease (HD) is a fatal neurodegenerative disorder caused by the expansion of a CAG trinucleotide repeat in the Huntingtin gene. Transcriptional dysregulation is one of the main cellular processes affected by mutant Huntingtin (mHtt). In this study, we investigate the alterations in miRNA and mRNA expression levels in a Drosophila model of HD by RNA sequencing and assess the functional effects of misregulated miRNAs in vivo. We found that in head samples of HD flies, the level of 32 miRNAs changed significantly; half of these were upregulated, while the other half were downregulated. After comparing miRNA and mRNA expression data, we discovered similarities in the impacted molecular pathways. Additionally, we observed that the putative targets of almost all dysregulated miRNAs were overrepresented among the upregulated mRNAs. We tested the effects of overexpression of five misregulated miRNAs in the HD model and found that while mir-10 and mir-219 enhanced, mir-137, mir-305, and mir-1010 ameliorated mHtt-induced phenotypes. Based on our results, we propose that while altered expression of mir-10, mir-137, and mir-1010 might be part of HD pathology, the upregulation of mir-305 might serve as a compensatory mechanism as a response to mHtt-induced transcriptional dysregulation. LA - English DB - MTMT ER -