@article{MTMT:34832088,
	title = {Exploring the interplay between the core microbiota, physicochemical factors, agrobiochemical cycles in the soil of the historic tokaj mád wine region},
	url = {https://m2.mtmt.hu/api/publication/34832088},
	author = {Gálné Remenyik, Judit and Csige, László and Dávid, Péter and Fauszt, Péter and Szilágyi-Rácz, Anna Anita and Szőllősi, Erzsébet and Bacsó, Zsófia Réka and Szepsy Jnr, István and Molnár, Krisztina and Rácz, Csaba and Fidler, Gábor and Kállai, Zoltán and Stündl, László and Dobos, Attila Csaba and Paholcsek, Melinda},
	doi = {10.1371/journal.pone.0300563},
	journal-iso = {PLOS ONE},
	journal = {PLOS ONE},
	volume = {19},
	unique-id = {34832088},
	issn = {1932-6203},
	abstract = {A Hungarian survey of Tokaj-Mád vineyards was conducted. Shotgun metabarcoding was applied to decipher the microbial-terroir. The results of 60 soil samples showed that there were three dominant fungal phyla, Ascomycota 66.36% ± 15.26%, Basidiomycota 18.78% ± 14.90%, Mucoromycota 11.89% ± 8.99%, representing 97% of operational taxonomic units (OTUs). Mutual interactions between microbiota diversity and soil physicochemical parameters were revealed. Principal component analysis showed descriptive clustering patterns of microbial taxonomy and resistance gene profiles in the case of the four historic vineyards (Szent Tamás, Király, Betsek, Nyúlászó). Linear discriminant analysis effect size was performed, revealing pronounced shifts in community taxonomy based on soil physicochemical properties. Twelve clades exhibited the most significant shifts (LDA > 4.0), including the phyla Verrucomicrobia , Bacteroidetes , Chloroflexi , and Rokubacteria , the classes Acidobacteria , Deltaproteobacteria , Gemmatimonadetes , and Betaproteobacteria , the order Sphingomonadales , Hypomicrobiales , as well as the family Sphingomonadaceae and the genus Sphingomonas . Three out of the four historic vineyards exhibited the highest occurrences of the bacterial genus Bradyrhizobium , known for its positive influence on plant development and physiology through the secretion of steroid phytohormones. During ripening, the taxonomical composition of the soil fungal microbiota clustered into distinct groups depending on altitude, differences that were not reflected in bacteriomes. Network analyses were performed to unravel changes in fungal interactiomes when comparing postveraison and preharvest samples. In addition to the arbuscular mycorrhiza Glomeraceae , the families Mycosphaerellacae and Rhyzopodaceae and the class Agaricomycetes were found to have important roles in maintaining soil microbial community resilience. Functional metagenomics showed that the soil Na content stimulated several of the microbiota-related agrobiogeochemical cycles, such as nitrogen and sulphur metabolism; steroid, bisphenol, toluene, dioxin and atrazine degradation and the synthesis of folate.},
	year = {2024},
	eissn = {1932-6203},
	orcid-numbers = {Szilágyi-Rácz, Anna Anita/0000-0003-3612-3574; Molnár, Krisztina/0000-0001-6203-6154; Rácz, Csaba/0000-0002-0307-6508; Dobos, Attila Csaba/0000-0001-7904-2155}
}

@article{MTMT:34744049,
	title = {Reinstatement of the fission yeast species Schizosaccharomyces versatilis Wickerham et Duprat, a sibling species of Schizosaccharomyces japonicus},
	url = {https://m2.mtmt.hu/api/publication/34744049},
	author = {Brysch-Herzberg, M. and Jia, G.-S. and Sipiczki, Mátyás and Seidel, M. and Zhang, W.-C. and Du, L.-L.},
	doi = {10.1002/yea.3922},
	journal-iso = {YEAST},
	journal = {YEAST},
	volume = {41},
	unique-id = {34744049},
	issn = {0749-503X},
	abstract = {Schizosaccharomyces japonicus Yukawa et Maki (1931) and Schizosaccharomyces versatilis Wickerham et Duprat (1945) have been treated as varieties of S. japonicus or as conspecific, based on various approaches including mating trials and nDNA/nDNA optical reassociation studies. However, the type strains of S. japonicus and S. versatilis differ by five substitutions (99.15% identity) and one 1-bp indel in the sequences of the D1/D2 domain of the 26S rRNA gene, and 23 substitutions (96.3% identity) and 31-bp indels in the sequences of internal transcribed spacer (ITS) of rRNA, suggesting that they may not be conspecific. To reassess their taxonomic status, we conducted mating trials and whole-genome analyses. Mating trials using the type strains showed a strong but incomplete prezygotic sterility barrier, yielding interspecies mating products at two orders of magnitude lower efficiency than intraspecies matings. These mating products, which were exclusively allodiploid hybrids, were unable to undergo the haplontic life cycle of the parents. We generated chromosome-level gap-less genome assemblies for both type strains. Whole genome sequences yielded an average nucleotide identity (ANI) of 86.4%, indicating clear separation of S. japonicus and S. versatilis. Based on these findings, we propose the reinstatement of S. versatilis as a distinct species (holotype strain: CBS 103T and ex-types: NRRL Y-1026, NBRC 1607, ATCC 9987, PYCC 7100; Mycobank no.: 847838).},
	keywords = {GENOMIC REARRANGEMENTS; reproductive incompatibility; Schizosaccharomyces versatilis},
	year = {2024},
	eissn = {1097-0061},
	pages = {108-127},
	orcid-numbers = {Sipiczki, Mátyás/0000-0002-1059-1305}
}

@article{MTMT:34767238,
	title = {Examinations of cellular uptake of cell penetrating peptides in vitro and in vivo},
	url = {https://m2.mtmt.hu/api/publication/34767238},
	author = {Toth, Gabriella and Batta, Gyula Gábor (Ifj.) and Karpati, Levente and Szöőr, Árpád and Mandity, Istvan and Nagy, Péter},
	journal-iso = {EUR BIOPHYS J},
	journal = {EUROPEAN BIOPHYSICS JOURNAL},
	volume = {52},
	unique-id = {34767238},
	issn = {0175-7571},
	year = {2023},
	eissn = {1432-1017},
	pages = {S163-S163},
	orcid-numbers = {Batta, Gyula Gábor (Ifj.)/0000-0001-8735-6920; Nagy, Péter/0000-0002-7466-805X}
}

@CONFERENCE{MTMT:34453415,
	title = {Comprehensive functional analyses of the bzip transciption factors AtfA and AtfB in Aspergillus Nidulans},
	url = {https://m2.mtmt.hu/api/publication/34453415},
	author = {Kocsis, Beatrix and Mi-Kyung, Lee and Jae-Hyuk, Yu and Nagy, Tibor and Daróczi, Lajos and Batta, Gyula Gábor (Ifj.) and Pócsi, István and Leiter, Éva Juliánna},
	booktitle = {16th European Conference on Fungal Genetics: Programme & Abstracts},
	unique-id = {34453415},
	year = {2023},
	pages = {705-706},
	orcid-numbers = {Nagy, Tibor/0000-0001-8568-914X; Batta, Gyula Gábor (Ifj.)/0000-0001-8735-6920}
}

@CONFERENCE{MTMT:34448404,
	title = {Recent results in Fusarium verticillioides research at the University of Debrecen},
	url = {https://m2.mtmt.hu/api/publication/34448404},
	author = {Pócsi, István and Pákozdi, Klaudia Gréta and Miskei, Márton and Benkő , Zsigmond and Papp, László Attila and Pusztahelyi, Tünde and Virág, Eszter Andrea},
	booktitle = {7th International Conference on Emerging Trends and Innovations in Biotechnology BIOSPECTRUM 2023: Souvenir and Abstracts.},
	unique-id = {34448404},
	year = {2023},
	pages = {19},
	orcid-numbers = {Pusztahelyi, Tünde/0000-0002-5495-6273}
}

@CONFERENCE{MTMT:34447547,
	title = {Biological elimination of Fusarium mycotoxins: yeasts and bacteria in work},
	url = {https://m2.mtmt.hu/api/publication/34447547},
	author = {Pusztahelyi, Tünde and Kovács, Szilvia and Adácsi, Cintia and Miklós, Ida and Pócsi, István},
	booktitle = {International Conference on New Horizons in Biotechnology ABSTRACT BOOK},
	unique-id = {34447547},
	year = {2023},
	pages = {144},
	orcid-numbers = {Pusztahelyi, Tünde/0000-0002-5495-6273; Miklós, Ida/0000-0002-7074-4019}
}

@article{MTMT:34410030,
	title = {Identification of antagonistic yeasts as potential biocontrol agents: Diverse criteria and strategies},
	url = {https://m2.mtmt.hu/api/publication/34410030},
	author = {Sipiczki, Mátyás},
	doi = {10.1016/j.ijfoodmicro.2023.110360},
	journal-iso = {INT J FOOD MICROBIOL},
	journal = {INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY},
	volume = {406},
	unique-id = {34410030},
	issn = {0168-1605},
	abstract = {Plant pathogenic and food spoilage microorganisms cause serious losses in crop production and severe damage during food manufacturing, transportation and storage. Synthetic antimicrobial agents are commonly used to control their propagation and harmful activities. However, the recent trend is shifting from chemicals towards safer and more eco-friendly alternatives. The use of antagonistic microorganisms as biological antimicrobial agents is becoming popular throughout the world to replace chemical agents. High numbers of microorganisms have turned out to exert adverse/inhibitory effects on other microorganisms including pathogens and spoiling strains. However, most of them are only active under laboratory conditions and their activity is sensitive to environmental changes. Only a small number of them can be used to manufacture biological protective products on an industrial scale. Therefore, there is a great need to identify additional antagonists. Yeasts have come to the forefront of attention because antimicrobial antagonism is fairly widespread among them. In the recent years, numerous excellent review articles covered various aspects of the phenomenon of antimicrobial antagonism of yeasts. However, none of them dealt with how antagonistic yeasts can be sought and identified, despite the high number and diverse efficiency of screening and identification procedures. As researchers working in different laboratories use different criteria and different experimental set-ups, a yeast strain found antagonistic in one laboratory may prove to be non-antagonistic in another laboratory. This review aims to provide a comprehensive and partially critical overview of the wide diversity of identification criteria and procedures to help researchers choose appropriate screening and identification strategies.},
	year = {2023},
	eissn = {1879-3460},
	orcid-numbers = {Sipiczki, Mátyás/0000-0002-1059-1305}
}

@article{MTMT:34129658,
	title = {Cloning, expression, and biochemical characterisation of a novel endomannanase from Thermobifida alba},
	url = {https://m2.mtmt.hu/api/publication/34129658},
	author = {Luzics, Szabina and Tóth, Ákos and Barna, Teréz and Szabó, Erna and Nagy, I. and Horváth, B. and Nagy, István and Varecza, Zoltán and Batáné Vidács, Ildikó and Kukolya, József},
	doi = {10.1556/066.2023.00186},
	journal-iso = {ACTA ALIMENT},
	journal = {ACTA ALIMENTARIA: AN INTERNATIONAL JOURNAL OF FOOD SCIENCE},
	volume = {52},
	unique-id = {34129658},
	issn = {0139-3006},
	year = {2023},
	eissn = {1588-2535},
	pages = {502-519},
	orcid-numbers = {Batáné Vidács, Ildikó/0000-0003-3019-8030; Kukolya, József/0000-0002-0724-9541}
}

@article{MTMT:34081680,
	title = {Antifungal Activity of Glucosinolate-Derived Nitriles and Their Synergistic Activity with Glucosinolate-Derived Isothiocyanates Distinguishes Various Taxa of Brassicaceae Endophytes and Soil Fungi},
	url = {https://m2.mtmt.hu/api/publication/34081680},
	author = {Szűcs, Zsolt and Plaszkó, Tamás and Bódor, Eszter and Csoma, Hajnalka and Ács-Szabó, Lajos and Kiss, Attila and Vasas, Gábor and Gonda, Sándor},
	doi = {10.3390/plants12142741},
	journal-iso = {PLANTS-BASEL},
	journal = {PLANTS-BASEL},
	volume = {12},
	unique-id = {34081680},
	abstract = {The glucosinolates of Brassicaceae plants are converted into bioactive isothiocyanates and other volatiles during a challenge by pathogens and other biotic stressors. However, the role of alternative downstream products with weaker potency (e.g., nitriles) is far from being fully understood. This study tested the possible synergistic antifungal interaction between various glucosinolate-derived nitriles and 2-phenylethyl isothiocyanate (PEITC) on 45 fungal strains, including endophytes from horseradish roots (Brassicaceae) and soil fungi, using an airtight system enabling the accurate study of extremely volatile antifungal agents. The median minimal inhibitory concentrations (MICs) were 1.28, 6.10, 27.00 and 49.72 mM for 1H-indole-3-acetonitrile (IAN), 3-phenylpropanenitrile (PPN), 4-(methylsulfanyl)-butanenitrile (MSBN) and 3-butenenitrile (BN, = allyl cyanide), respectively. Thus, nitriles were considerably weaker antifungal agents compared to PEITC with a median MIC of 0.04 mM. For the same nitriles, the median fractional inhibitory concentration indices (FICIs) of the combinations were 0.562, 0.531, 0.562 and 0.625, respectively. Altogether, 47.7%, 56.8%, 50.0% and 27.3% of tested fungal strains showed a synergistic antifungal activity (FICI ≤ 0.5) for the nitrile–isothiocyanate combinations, respectively. Hypocreales strains showed the least sensitivity towards the GSL decomposition products and their combinations. The mean MIC values for PEITC showed 0.0679 ± 0.0358, 0.0400 ± 0.0214, 0.0319 ± 0.0087 and 0.0178 ± 0.0171 mM for Hypocreales, Eurotiales, Glomerellales and Pleosporales, respectively. In addition, nitriles, especially IAN, also showed significant differences. For the same fungi, the median FICI values fell in the ranges of 0.61–0.67, 0.52–0.61, 0.40–0.50 and 0.48–0.67, respectively, depending on the nitrile. Our results suggest that glucosinolate-derived nitriles may enhance isothiocyanate antifungal activity and that they may play an active role in shaping the plant microbiome and contribute to the filtering of microbes by plants.},
	keywords = {ISOTHIOCYANATE; Antifungal activity; nitrile; ITC; Synergy; Soil fungi; Endophyte fungi},
	year = {2023},
	eissn = {2223-7747},
	orcid-numbers = {Plaszkó, Tamás/0000-0003-4211-3823; Kiss, Attila/0000-0003-3601-5143; Gonda, Sándor/0000-0001-9776-0249}
}

@article{MTMT:34026705,
	title = {Schizosaccharomyces lindneri sp. nov., a fission yeast occurring in honey},
	url = {https://m2.mtmt.hu/api/publication/34026705},
	author = {Brysch-Herzberg, Michael and Jia, Guo-Song and Sipiczki, Mátyás and Seidel, Martin and Li, Wen and Assali, Imen and Du, Li-Lin},
	doi = {10.1002/yea.3857},
	journal-iso = {YEAST},
	journal = {YEAST},
	volume = {40},
	unique-id = {34026705},
	issn = {0749-503X},
	abstract = {Two strains of fission yeast were isolated from honey. They differ from the type strain of Schizosaccharomyces octosporus by three substitutions in the D1/D2 domain of the nuclear 26S large subunit ribosomal RNA (rRNA) gene sequence, resulting in a 99.5% identity. In the internal transcribed spacer (ITS) region (consisting of ITS1, 5.8S rDNA, and ITS2), the strains differ from S. octosporus by 16 gaps and 91 substitutions, which is equivalent to an identity of 88.1%. Genome sequencing on one of the new strains revealed that the average nucleotide identity (ANI) between its genome and the reference genome of S. octosporus is 90.43% and there exist major genome rearrangements between the two genomes. Mating analysis revealed that S. octosporus and one of the new strains are completely reproductively separated. A strong prezygotic barrier exists and the few mating products consist of diploid hybrids that do not form recombinant ascospores. In the new strains, asci are either zygotic, arising from conjugation, or they develop without conjugation from asexual cells (azygotic). Compared to the currently recognized Schizosaccharomyces species, the spectrum of nutrients that are assimilated by the new strains is restricted. Of the 43 carbohydrates that were included in the physiological standard tests, only 7 were assimilated. According to the results of the genome sequence analysis, the mating trials, and the phenotypic characterization, the new species Schizosaccharomyces lindneri is described to accommodate the two strains (holotype: CBS 18203(T) and ex-type: MUCL 58363; MycoBank no.: MB 847838).},
	keywords = {MUTANTS; microbiology; HYBRIDIZATION; MULTIPLE SEQUENCE ALIGNMENT; GENOMIC REARRANGEMENTS; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; pombe; Schizosaccharomyces lindneri; MAUVE},
	year = {2023},
	eissn = {1097-0061},
	pages = {237-253},
	orcid-numbers = {Sipiczki, Mátyás/0000-0002-1059-1305}
}