@{MTMT:36332522, title = {Pharmaceutical aspects of conventional and recent developments for the treatment of metabolic diseases and disorders}, url = {https://m2.mtmt.hu/api/publication/36332522}, author = {Kósa, Dóra and Pető, Ágota and Bácskay, Ildikó}, booktitle = {Pharmacological Targets in Metabolic Diseases}, unique-id = {36332522}, year = {2026}, pages = {53-62} } @article{MTMT:35652942, title = {Fluoxetine exerts anti-proliferative effect in human epidermal keratinocytes}, url = {https://m2.mtmt.hu/api/publication/35652942}, author = {Ádám-Nagy, Dorottya and Arany, József and Tóth, Kinga Fanni and Póliska, Szilárd and Váradi, Judit and Kolozsi, Péter and Tóth, Dezső and Niehues, Hanna and van den Bogaard, Ellen H. and Soeberdt, Michael and Abels, Christoph and Oláh, Attila}, doi = {10.1007/s00403-024-03711-9}, journal-iso = {ARCH DERMATOL RES}, journal = {ARCHIVES OF DERMATOLOGICAL RESEARCH}, volume = {317}, unique-id = {35652942}, issn = {0340-3696}, abstract = {We have recently shown that fluoxetine (FX) suppressed polyinosinic-polycytidylic acid-induced inflammatory response and endothelin release in human epidermal keratinocytes, via the indirect inhibition of the phosphoinositide 3-kinase (PI3K)-pathway. Because PI3K-signaling is a positive regulator of the proliferation, in the current, highly focused followup study, we assessed the effects of FX (14 μM) on the proliferation and differentiation of human epidermal keratinocytes. We found that FX exerted anti-proliferative actions in 2D cultures (HaCaT and primary human epidermal keratinocytes [NHEKs]; 48- and 72-h; CyQUANT-assay) as well as in 3D reconstructed epidermal equivalents (48-h; Ki-67 immunohistochemistry). Importantly, FX did not influence epidermal thickness (hematoxylin-eosin staining), and it did not have a major impact on the differentiation-associated alteration of the gene expression pattern (24-h treatments; RNA-Seq). Moreover, neither keratin (K)-1, nor K10 expression was altered by FX in NHEKs (RT-qPCR) or in 3D epidermal equivalents (semi-quantitative immunohistomorphometry). FX did not influence differentiation-induced up-regulation of occludin (RT-qPCR; NHEKs), and did not alter differentiation-associated barrier forming capacity of epidermal keratinocytes (electrical impedance; Lucifer Yellow penetration assay). Our data indicate that, besides the previously reported combined anti-inflammatory and putative anti-pruritic effects, FX may also suppress proliferation of human epidermal keratinocytes without impairing their differentiation and barrier-forming capacity.}, year = {2025}, eissn = {1432-069X}, orcid-numbers = {Oláh, Attila/0000-0003-4122-5639} } @article{MTMT:35710602, title = {Development of Salvia officinalis–Based Self-Emulsifying Systems for Dermal Application: Antioxidant, Anti-Inflammatory, and Skin Penetration Enhancement}, url = {https://m2.mtmt.hu/api/publication/35710602}, author = {Bodnár, Krisztina and Papp, Boglárka and Sinka, Dávid and Siposné Fehér, Pálma and Ujhelyi, Zoltán and Lekli, István and Kajtár, Richárd and Nacsa, Fruzsina and Bácskay, Ildikó and Józsa, Liza}, doi = {10.3390/pharmaceutics17020140}, journal-iso = {PHARMACEUTICS}, journal = {PHARMACEUTICS}, volume = {17}, unique-id = {35710602}, abstract = {Background/Objectives: The present study focused on the formulation and evaluation of novel topical systems containing Salvia officinalis (sage), emphasizing their antioxidant and anti-inflammatory properties. Sage, rich in carnosol, offers considerable therapeutic potential, yet its low water solubility limits its effectiveness in traditional formulations. The aim of our experimental work was to improve the solubility and thus bioavailability of the active ingredient by developing self-nano/microemulsifying drug delivery systems (SN/MEDDSs) with the help of Labrasol and Labrafil M as the nonionic surfactants, Transcutol HP as the co-surfactant, and isopropyl myristate as the oily phase. Methods: The formulations were characterized for droplet size, zeta potential, polydispersity index (PDI), encapsulation efficacy, and stability. The composition exhibiting the most favorable characteristics, with particle sizes falling within the nanoscale range, was incorporated into a cream and a gel, which were compared for their textural properties, carnosol penetration, biocompatibility and efficacy. Results: Release studies conducted using Franz diffusion cells demonstrated that the SNEDDS-based cream achieved up to 80% carnosol release, outperforming gels. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) test and enzyme-linked immunosorbent assays (ELISA) showed strong efficacy, with an in vivo carrageenan-induced rat paw edema model revealing that the SNEDDS-based cream significantly reduced inflammation. Conclusions: These findings highlight the potential of SNEDDS-enhanced topical formulations in improving therapeutic outcomes. Further research is warranted to confirm their long-term safety and efficacy.}, year = {2025}, eissn = {1999-4923}, pages = {140}, orcid-numbers = {Nacsa, Fruzsina/0009-0005-0064-6180} } @article{MTMT:35710641, title = {Bioavailability Enhancement and Formulation Technologies of Oral Mucosal Dosage Forms: A Review}, url = {https://m2.mtmt.hu/api/publication/35710641}, author = {Bácskay, Ildikó and Arany, Petra and Siposné Fehér, Pálma and Józsa, Liza and Vasvári, Gábor and Nemes, Dániel and Pető, Ágota and Kósa, Dóra and Haimhoffer, Ádám and Ujhelyi, Zoltán and Sinka, Dávid}, doi = {10.3390/pharmaceutics17020148}, journal-iso = {PHARMACEUTICS}, journal = {PHARMACEUTICS}, volume = {17}, unique-id = {35710641}, abstract = {The oral mucosa is a versatile surface for drug administration, supporting both local and systemic therapies. Many active substances are effectively absorbed in the oral cavity, offering an alternative to enteral administration by bypassing the harsh gastrointestinal environment and hepatic first-pass metabolism. This has made oral mucosal drug delivery a growing area of research. Enhancing the bioavailability of active ingredients is a key focus in pharmaceutical technology, especially given the challenges of developing new drugs. Numerous strategies to improve bioavailability are compatible with oral mucosal delivery, with the unique anatomy of the oral cavity enabling specialized applications. A variety of dosage forms tailored for oral mucosal delivery meet therapeutic needs while addressing biopharmaceutical and patient compliance challenges. Proper formulation can achieve controlled release, improved bioavailability, and patient convenience. This review highlights the potential of oral mucosal drug delivery, focusing on bioavailability enhancement methods and the types and production technologies of dosage forms optimized for use in the oral cavity.}, year = {2025}, eissn = {1999-4923}, pages = {148}, orcid-numbers = {Nemes, Dániel/0000-0002-5477-0540} } @article{MTMT:35784145, title = {In Vitro Functional and Structural Evaluation of Low-Complexity Artificial Human Epidermis for 3D Tissue Engineering}, url = {https://m2.mtmt.hu/api/publication/35784145}, author = {Kocsis, Dorottya and Sztankovics, Dániel and Józsa, Liza and Németh, Afrodité and Garay, Tamás and Naszlady, Márton Bese and Lengyel, Miléna and Vecsernyés, Miklós and Antal, István and Sebestyén, Anna and Erdő, Franciska}, doi = {10.3390/bioengineering12030230}, journal-iso = {BIOENGINEERING-BASEL}, journal = {BIOENGINEERING}, volume = {12}, unique-id = {35784145}, abstract = {In recent times, with the need for a reduction, refinement, and replacement of in vivo animal testing, there has been an increasing demand for the use of relevant in vitro human cell systems in drug development. There is also a great demand for the replacement of skin tissue in various wounds and burns. Furthermore, human skin cell-based in vitro systems can be used to investigate the side effects (toxicity and irritation) and tissue penetration of topical preparations. In this study, exploratory experiments were performed to produce artificial epidermis using two hydrogel scaffolds, alginate and GelMA C. The amount of keratinocytes added to the matrix (10–50–100 × 106/mL) and the duration of tissue maturation (fresh, 1–3–4 weeks) were optimized in an extensive study. The behavior and structure of the two hydrogels were functionally and morphologically assessed. The permeability order for caffeine in the tested barriers was the following: alginate > GelMA C > cellulose acetate membrane > rat skin. It was concluded that GelMA C matrix provides a more favorable environment for cell survival and tissue differentiation (as demonstrated by histology and immunohistochemistry) than alginate. The 3-week incubation and 50 × 106/mL cell number proved to be the most beneficial in the given system. This study provides data for the first time on the multifactorial optimization of two potential skin substitutes for tissue manufacturing. In order to use these results in tissue engineering, the fabricated artificial epidermis preparations must also be optimized for biocompatibility and from physical and mechanical point of views.}, year = {2025}, eissn = {2306-5354}, orcid-numbers = {Józsa, Liza/0000-0001-5835-5304; Garay, Tamás/0000-0003-0329-9207; Naszlady, Márton Bese/0000-0001-8114-1750; Lengyel, Miléna/0000-0001-8865-054X; Antal, István/0000-0002-5434-201X; Sebestyén, Anna/0000-0001-8814-4794; Erdő, Franciska/0000-0001-6265-3777} } @article{MTMT:35804387, title = {AI-Driven Framework for Enhanced and Automated Behavioral Analysis in Morris Water Maze Studies}, url = {https://m2.mtmt.hu/api/publication/35804387}, author = {Lakatos, István and Bogacsovics, Gergő and Tiba, Attila and Priksz, Dániel and Juhász, Béla and Erdélyi, Rita and Berényi, Zsuzsa and Bácskay, Ildikó and Ujvárosy, Dóra and Harangi, Balázs}, doi = {10.3390/s25051564}, journal-iso = {SENSORS-BASEL}, journal = {SENSORS}, volume = {25}, unique-id = {35804387}, abstract = {The Morris Water Maze (MWM) is a widely used behavioral test to assess the spatial learning and memory of animals, particularly valuable in studying neurodegenerative disorders such as Alzheimer’s disease. Traditional methods for analyzing MWM experiments often face limitations in capturing the complexity of animal behaviors. In this study, we present a novel AI-based automated framework to process and evaluate MWM test videos, aiming to enhance behavioral analysis through machine learning. Our pipeline involves video preprocessing, animal detection using convolutional neural networks (CNNs), trajectory tracking, and postprocessing to derive detailed behavioral features. We propose concentric circle segmentation of the pool next to the quadrant-based division, and we extract 32 behavioral metrics for each zone, which are employed in classification tasks to differentiate between younger and older animals. Several machine learning classifiers, including random forest and neural networks, are evaluated, with feature selection techniques applied to improve the classification accuracy. Our results demonstrate a significant improvement in classification performance, particularly through the integration of feature sets derived from concentric zone analyses. This automated approach offers a robust solution for MWM data processing, providing enhanced precision and reliability, which is critical for the study of neurodegenerative disorders.}, year = {2025}, eissn = {1424-8220}, orcid-numbers = {Lakatos, István/0000-0002-1451-6046; Harangi, Balázs/0000-0003-4405-2040} } @article{MTMT:35960147, title = {Correction: Fluoxetine exerts anti-proliferative effect in human epidermal keratinocytes}, url = {https://m2.mtmt.hu/api/publication/35960147}, author = {Ádám-Nagy, Dorottya and Arany, József and Tóth, Kinga Fanni and Póliska, Szilárd and Váradi, Judit and Kolozsi, Péter and Tóth, Dezső and Niehues, Hanna and Bogaard, Elen H. Van den and Soeberdt, Michael and Abels, Christoph and Oláh, Attila}, doi = {10.1007/s00403-025-04001-8}, journal-iso = {ARCH DERMATOL RES}, journal = {ARCHIVES OF DERMATOLOGICAL RESEARCH}, volume = {317}, unique-id = {35960147}, issn = {0340-3696}, year = {2025}, eissn = {1432-069X}, orcid-numbers = {Niehues, Hanna/0000-0002-6954-6955; Bogaard, Elen H. Van den/0000-0003-4846-0287; Soeberdt, Michael/0000-0003-2594-9901; Abels, Christoph/0000-0002-7778-7740; Oláh, Attila/0000-0003-4122-5639} } @article{MTMT:36201062, title = {Tapinarof Nanogels as a Promising Therapeutic Approach}, url = {https://m2.mtmt.hu/api/publication/36201062}, author = {Balogh, Barbara Nóra and Pető, Ágota and Siposné Fehér, Pálma and Ujhelyi, Zoltán and Bácskay, Ildikó}, doi = {10.3390/pharmaceutics17060731}, journal-iso = {PHARMACEUTICS}, journal = {PHARMACEUTICS}, volume = {17}, unique-id = {36201062}, abstract = {Psoriasis is a chronic inflammatory skin disease characterised by increased oxidative stress, the overproliferation of keratinocytes, the accumulation of inflammatory mediators, and skin barrier damage. Although a number of therapeutic options are available, finding long-term treatments that are well-tolerated and patient-friendly treatments remains a challenge. Tapinarof is a new type of aryl hydrocarbon receptor (AhR) modulator that has recently attracted attention as a promising non-steroidal alternative. However, its application may be limited by its poor water solubility and low degree of skin penetration. Nanotechnology-based drug carriers, specially nanogels, offer new opportunities to overcome these limitations by combining the advantages of targeted drug delivery and enhanced skin penetration. Furthermore, nanogel formulations can improve skin hydration and support the restoration of skin barrier function, which are important in the treatment of psoriasis. This review focuses on current and emerging therapeutic approaches, with particular emphasis on the potential of incorporating tapinarof into nanogel formulations as a novel alternative to topical psoriasis treatment.}, year = {2025}, eissn = {1999-4923}, pages = {731} } @article{MTMT:36234894, title = {Formulation and investigation of Lactobacillus rhamnosus cek-R1 filled alginate microspheres with different excipients}, url = {https://m2.mtmt.hu/api/publication/36234894}, author = {Papp, Boglárka and Józsa, Liza and Sinka, Dávid and Fidrus, Eszter and Kardos, Gábor and Nacsa, Fruzsina and Bácskay, Ildikó and Siposné Fehér, Pálma}, doi = {10.71116/ws5t3n82}, journal = {DE REMEDIIS}, volume = {1}, unique-id = {36234894}, abstract = {Microspheres are spherical particles containing the active substance, in our case bacterial probiotic strain Lactobacillus rhamnosus (L. rhamnosus), in an individually coated form. L. rhamnosus is a natural constituent of the human intestinal flora and is known to improve immune function, enhance healing of the intestinal mucosa, reduce inflammation, bloating and diarrhoea. The aim of our experimental work was to formulate sodium alginate microspheres containing L. rhamnosus bacterial strain as active ingredient and prebiotic (galactooligosaccharide, pectin, inulin). The microspheres were formulated using Büchi Encapsulator equipment, after which the entrapment efficiency was measured. The lyophilized product was filled into hydroxypropylmethylcellulose (HPMC) capsules and was subjected to a dissolution assay using Erweka equipment. The number of viable L. rhamnosus was determined from samples of the dissolution fluid. The microspheres are lyophilised to improve shelf-life and facilitate filling into traditional capsules. The number of viable bacteria in the lyophilizate was determined by inoculation on medium and standard microdilution test. Microspheres containing different compositions of pro- and prebiotics were formulated, and their antioxidant capacity was detected by 2,2-diphenyl-l-1-picrylhydrazyl (DPPH). We tested the anti-inflammatory effect of the microspheres using human IL-4 ELISA Kit on colon adenocarcinoma (CaCo-2) cell line.}, year = {2025}, eissn = {3058-0358}, pages = {194}, orcid-numbers = {Nacsa, Fruzsina/0009-0005-0064-6180} } @article{MTMT:36283701, title = {Sex differences in suspected adverse drug reactions of anti-seizure medications reported in EudraVigilance}, url = {https://m2.mtmt.hu/api/publication/36283701}, author = {Girgis, Michael Magdy Fahmy and Farkasinszky, Gergely and Fekete, Klára and Fekete, István and Vecsernyés, Miklós and Bácskay, Ildikó and Horváth, László}, doi = {10.1016/j.ejps.2025.107119}, journal-iso = {EUR J PHARM SCI}, journal = {EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES}, volume = {210}, unique-id = {36283701}, issn = {0928-0987}, year = {2025}, eissn = {1879-0720}, pages = {107119}, orcid-numbers = {Horváth, László/0000-0003-1466-7052} }