TY - JOUR AU - Antal, Dóra AU - Pór, Ágnes AU - Kovács, Ilona AU - Dull, Katalin AU - Póliska, Szilárd AU - Ujlaki, Gyula AU - Demény, Máté Ágoston AU - Szöllősi, Attila AU - Kiss, Borbála AU - Szegedi, Andrea AU - Bay, Péter AU - Szántó, Magdolna TI - PARP2 promotes inflammation in psoriasis by modulating estradiol biosynthesis in keratinocytes JF - JOURNAL OF MOLECULAR MEDICINE-JMM J2 - J MOL MED-JMM VL - 101 PY - 2023 SP - 987 EP - 999 PG - 13 SN - 0946-2716 DO - 10.1007/s00109-023-02338-z UR - https://m2.mtmt.hu/api/publication/34034279 ID - 34034279 LA - English DB - MTMT ER - TY - JOUR AU - Guti, Eliza AU - Regdon, Zsolt AU - Sturniolo, Isotta AU - Kiss, Alexandra AU - Kovács, Katalin AU - Demény, Máté Ágoston AU - Szöőr, Árpád AU - Vereb, György AU - Szöllősi, János AU - Hegedűs, Csaba AU - Polgár, Zsuzsanna AU - Virág, László TI - The multitargeted receptor tyrosine kinase inhibitor sunitinib induces resistance of HER2 positive breast cancer cells to trastuzumab-mediated ADCC JF - CANCER IMMUNOLOGY IMMUNOTHERAPY J2 - CANCER IMMUNOL IMMUN VL - 71 PY - 2022 IS - 9 SP - 2151 EP - 2168 PG - 18 SN - 1432-0851 DO - 10.1007/s00262-022-03146-z UR - https://m2.mtmt.hu/api/publication/32628668 ID - 32628668 AB - Despite recent advances in the development of novel personalized therapies, breast cancer continues to challenge physicians with resistance to various advanced therapies. The anticancer action of the anti-HER2 antibody, trastuzumab, involves antibody-dependent cell-mediated cytotoxicity (ADCC) by natural killer (NK) cells. Here, we report a repurposing screen of 774 clinically used compounds on NK-cell + trastuzumab-induced killing of JIMT-1 breast cancer cells. Using a calcein-based high-content screening (HCS) assay for the image-based quantitation of ADCC that we have developed and optimized for this purpose, we have found that the multitargeted tyrosine kinase inhibitor sunitinib inhibits ADCC in this model. The cytoprotective effect of sunitinib was also confirmed with two other assays (lactate dehydrogenase release, and electric cell substrate impedance sensing, ECIS). The drug suppressed NK cell activation as indicated by reduced granzyme B deposition on to the target cells and inhibition of interferon-γ production by the NK cells. Moreover, sunitinib induced downregulation of HER2 on the target cells' surface, changed the morphology and increased adherence of the target cells. Moreover, sunitinib also triggered the autophagy pathway (speckled LC3b) as an additional potential underlying mechanism of the cytoprotective effect of the drug. Sunitinib-induced ADCC resistance has been confirmed in a 3D tumor model revealing the prevention of apoptotic cell death (Annexin V staining) in JIMT-1 spheroids co-incubated with NK cells and trastuzumab. In summary, our HCS assay may be suitable for the facile identification of ADCC boosting compounds. Our data urge caution concerning potential combinations of ADCC-based immunotherapies and sunitinib. LA - English DB - MTMT ER - TY - JOUR AU - Curtin, Nicola AU - Bay, Péter TI - PARPs, PAR and NAD Metabolism and Their Inhibitors in Cancer JF - CANCERS J2 - CANCERS VL - 12 PY - 2020 IS - 12 SP - 3494 SN - 2072-6694 DO - 10.3390/cancers12123494 UR - https://m2.mtmt.hu/api/publication/31672523 ID - 31672523 LA - English DB - MTMT ER - TY - JOUR AU - Aladdin, Azzam AU - Yao, Yanhua AU - Yang, Ciyu AU - Kahlert, Günther AU - Ghani, Marvi AU - Király, Nikolett AU - Boratkó, Anita AU - Uray (Davis), Karen L. AU - Dittmar, Gunnar AU - Tar, Krisztina TI - The Proteasome Activators Blm10/PA200 Enhance the Proteasomal Degradation of N-Terminal Huntingtin JF - BIOMOLECULES J2 - BIOMOLECULES VL - 10 PY - 2020 IS - 11 SP - 1581 SN - 2218-273X DO - 10.3390/biom10111581 UR - https://m2.mtmt.hu/api/publication/31669676 ID - 31669676 LA - English DB - MTMT ER - TY - JOUR AU - Sári, Zsanett Mercédesz AU - Mikó, Edit AU - Kovács, Tünde AU - Boratkó, Anita AU - Ujlaki, Gyula AU - Jankó, Laura AU - Kiss, Borbála Katalin AU - Uray (Davis), Karen L. AU - Bay, Péter TI - Indoxylsulfate, a metabolite of the microbiome, has cytostatic effects in breast cancer via activation of AHR and PXR receptors and induction of oxidative stress JF - CANCERS J2 - CANCERS VL - 12 PY - 2020 IS - 10 PG - 23 SN - 2072-6694 DO - 10.3390/cancers12102915 UR - https://m2.mtmt.hu/api/publication/31627406 ID - 31627406 N1 - Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Egyetem tér 1., Debrecen, 4032, Hungary MTA-DE Lendület Laboratory of Cellular Metabolism, Debrecen, 4032, Hungary Department of Oncology, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary Research Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, 4032, Hungary Cited By :2 Export Date: 24 May 2021 Correspondence Address: Bai, P.; Department of Medical Chemistry, Egyetem tér 1., Hungary; email: baip@med.unideb.hu Correspondence Address: Bai, P.; MTA-DE Lendület Laboratory of Cellular MetabolismHungary; email: baip@med.unideb.hu Correspondence Address: Bai, P.; Research Center for Molecular Medicine, Hungary; email: baip@med.unideb.hu Funding details: ÚNKP-19-4-DE-79, ÚNKP-20-5-DE-96 Funding details: Funding details: ÚNKP-20-4-II-DE-68 Funding details: NKFIH-1150-6/2019 LA - English DB - MTMT ER - TY - JOUR AU - Sári, Zsanett Mercédesz AU - Kovács, Tünde AU - Csonka, Tamás AU - Török, M AU - Sebő, É AU - Toth, J AU - Tóth, Dezső AU - Mikó, Edit AU - Kiss, Borbála Katalin AU - Szeőcs, Dóra AU - Uray (Davis), Karen L. AU - Karányi, Zsolt AU - Kovács, Ilona AU - Méhes, Gábor AU - Árkosy, Péter AU - Bay, Péter TI - Fecal expression of Escherichia coli lysine decarboxylase (LdcC) is downregulated in E-cadherin negative lobular breast carcinoma JF - PHYSIOLOGY INTERNATIONAL J2 - PHYSIOL INT VL - 107 PY - 2020 IS - 2 SP - 349 EP - 358 PG - 10 SN - 2498-602X DO - 10.1556/2060.2020.00016 UR - https://m2.mtmt.hu/api/publication/31607797 ID - 31607797 LA - English DB - MTMT ER - TY - JOUR AU - Nagy, Lilla Nikoletta AU - Béke, F AU - Juhász, László AU - Kovács, Tünde AU - Juhászné Tóth, Éva AU - Docsa, Tibor AU - Tóth, Attila AU - Somsák, László AU - Bay, Péter TI - Glycogen phosphorylase inhibitor, 2,3-bis[(2E)-3-(4-hydroxyphenyl)prop-2-enamido] butanedioic acid (BF142), improves baseline insulin secretion of MIN6 insulinoma cells JF - PLOS ONE J2 - PLOS ONE VL - 15 PY - 2020 IS - 9 PG - 15 SN - 1932-6203 DO - 10.1371/journal.pone.0236081 UR - https://m2.mtmt.hu/api/publication/31408732 ID - 31408732 N1 - Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Department of Organic Chemistry, Faculty of Science and Technology, University of Debrecen, Debrecen, Hungary Department of Cardiology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary MTA-DE Lendület Laboratory of Cellular Metabolism, Debrecen, Hungary Research Center for Molecular Medicine, University of Debrecen, Debrecen, Hungary Cited By :1 Export Date: 24 May 2021 CODEN: POLNC Correspondence Address: Bai, P.; Department of Medical Chemistry, Hungary; email: baip@med.unideb.hu AB - Type 2 diabetes mellitus (T2DM), one of the most common metabolic diseases, is characterized by insulin resistance and inadequate insulin secretion of beta cells. Glycogen phosphorylase (GP) is the key enzyme in glycogen breakdown, and contributes to hepatic glucose production during fasting or during insulin resistance. Pharmacological GP inhibitors are potential glucose lowering agents, which may be used in T2DM therapy. A natural product isolated from the cultured broth of the fungal strain No. 138354, called 2,3-bis(4-hydroxycinnamoyloxy)glutaric acid (FR258900), was discovered a decade ago. In vivo studies showed that FR258900 significantly reduced blood glucose levels in diabetic mice. We previously showed that GP inhibitors can potently enhance the function of beta cells. The purpose of this study was to assess whether an analogue of FR258900 can influence beta cell function. BF142 (Meso-Dimethyl 2,3-bis[(E)-3-(4-acetoxyphenyl)prop-2-enamido]butanedioate) treatment activated the glucose-stimulated insulin secretion pathway, as indicated by enhanced glycolysis, increased mitochondrial oxidation, significantly increased ATP production, and elevated calcium influx in MIN6 cells. Furthermore, BF142 induced mTORC1-specific phosphorylation of S6K, increased levels of PDX1 and insulin protein, and increased insulin secretion. Our data suggest that BF142 can influence beta cell function and can support the insulin producing ability of beta cells. LA - English DB - MTMT ER - TY - JOUR AU - Sári, Zsanett Mercédesz AU - Mikó, Edit AU - Kovács, Tünde AU - Jankó, Laura AU - Csonka, Tamás AU - Lente, G AU - Sebő, É AU - Toth, J AU - Tóth, Dezső AU - Árkosy, Péter AU - Boratkó, Anita AU - Ujlaki, Gyula AU - Török, M AU - Kovács, Ilona AU - Szabó, Judit AU - Kiss, Borbála Katalin AU - Méhes, Gábor AU - Goedert, JJ AU - Bay, Péter TI - Indolepropionic acid, a metabolite of the microbiome, has cytostatic properties in breast cancer by activating AHR and PXR receptors and inducing oxidative stress JF - CANCERS J2 - CANCERS VL - 12 PY - 2020 IS - 9 PG - 27 SN - 2072-6694 DO - 10.3390/cancers12092411 UR - https://m2.mtmt.hu/api/publication/31408725 ID - 31408725 LA - English DB - MTMT ER - TY - JOUR AU - Mészáros, Beáta AU - Papp, Ferenc AU - Mocsár, Gábor AU - Kókai, Endre AU - Kovács, Katalin AU - Tajti, Gábor AU - Panyi, György TI - The voltage-gated proton channel hHv1 is functionally expressed in human chorion-derived mesenchymal stem cells JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 10 PY - 2020 IS - 1 SN - 2045-2322 DO - 10.1038/s41598-020-63517-3 UR - https://m2.mtmt.hu/api/publication/31334758 ID - 31334758 LA - English DB - MTMT ER - TY - JOUR AU - DOUIDA, ABDENNOUR AU - Batista, F AU - Robaszkiewicz, A AU - Botó, Pál AU - Aladdin, Azzam AU - Szenykiv, M AU - Czinege, R AU - Virág, László AU - Tar, Krisztina TI - The proteasome activator PA200 regulates expression of genes involved in cell survival upon selective mitochondrial inhibition in neuroblastoma cells JF - JOURNAL OF CELLULAR AND MOLECULAR MEDICINE J2 - J CELL MOL MED VL - 24 PY - 2020 IS - 12 SP - 6716 EP - 6730 PG - 1 SN - 1582-1838 DO - 10.1111/jcmm.15323 UR - https://m2.mtmt.hu/api/publication/31307373 ID - 31307373 LA - English DB - MTMT ER -