@article{MTMT:35201514,
	title = {Exploring the transcriptomic profile of human monkeypox virus via CAGE and native RNA sequencing approaches},
	url = {https://m2.mtmt.hu/api/publication/35201514},
	author = {Nagy, Gergely Ármin and Tombácz, Dóra and Prazsák, István and Csabai, Zsolt and Dörmő, Ákos and Gulyás, Gábor and Kemenesi, Gábor and Tóth, Gábor Endre and Holoubek, Jiří and Růžek, Daniel and Kakuk, Balázs and Boldogkői, Zsolt},
	doi = {10.1128/msphere.00356-24},
	journal-iso = {MSPHERE},
	journal = {mSPHERE},
	unique-id = {35201514},
	issn = {2379-5042},
	year = {2024},
	eissn = {2379-5042},
	orcid-numbers = {Tombácz, Dóra/0000-0001-5520-2978; Csabai, Zsolt/0000-0003-0031-0116; Kemenesi, Gábor/0000-0001-9775-3065; Tóth, Gábor Endre/0000-0002-7201-9646; Boldogkői, Zsolt/0000-0003-1184-7293}
}

@article{MTMT:35159268,
	title = {Single-cell level LasR-mediated quorum sensing response of Pseudomonas aeruginosa to pulses of signal molecules},
	url = {https://m2.mtmt.hu/api/publication/35159268},
	author = {Ábrahám, Ágnes and Dér, László and Csákvári, Eszter and Vizsnyiczai, Gaszton and Pap, Imre and Lukacs, Rebeka and Varga-Zsíros, Vanda and Nagy, Krisztina and Galajda, Péter},
	doi = {10.1038/s41598-024-66706-6},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {14},
	unique-id = {35159268},
	issn = {2045-2322},
	abstract = {Quorum sensing (QS) is a communication form between bacteria via small signal molecules that enables global gene regulation as a function of cell density. We applied a microfluidic mother machine to study the kinetics of the QS response of Pseudomonas aeruginosa bacteria to additions and withdrawals of signal molecules. We traced the fast buildup and the subsequent considerably slower decay of a population-level and single-cell-level QS response. We applied a mathematical model to explain the results quantitatively. We found significant heterogeneity in QS on the single-cell level, which may result from variations in quorum-controlled gene expression and protein degradation. Heterogeneity correlates with cell lineage history, too. We used single-cell data to define and quantitatively characterize the population-level quorum state. We found that the population-level QS response is well-defined. The buildup of the quorum is fast upon signal molecule addition. At the same time, its decay is much slower following signal withdrawal, and the quorum may be maintained for several hours in the absence of the signal. Furthermore, the quorum sensing response of the population was largely repeatable in subsequent pulses of signal molecules.},
	keywords = {green fluorescent protein; BACTERIA; COMMUNICATION; MATHEMATICAL-MODEL; BIOLUMINESCENCE; VIBRIO-FISCHERI; PHENOTYPIC HETEROGENEITY; Association rates; STOCHASTIC GENE-EXPRESSION},
	year = {2024},
	eissn = {2045-2322},
	orcid-numbers = {Vizsnyiczai, Gaszton/0000-0003-3245-3736}
}

@article{MTMT:34883229,
	title = {LINE-1 ORF1p is a Promising Biomarker in Cervical Intraepithelial Neoplasia Degree Assessment},
	url = {https://m2.mtmt.hu/api/publication/34883229},
	author = {Karkas, Réka and Abdullah, Khaldoon Sadiq Ahmed and Kaizer, László and Ürmös, A and Raya, May and Tiszlavicz, Lilla Györgyi and Pankotai, Tibor and Nagy, István and Mátés, Lajos and Sükösd, Farkas},
	doi = {10.1097/PGP.0000000000001035},
	journal-iso = {INT J GYNECOL PATHOL},
	journal = {INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY},
	volume = {AiP},
	unique-id = {34883229},
	issn = {0277-1691},
	year = {2024},
	eissn = {1538-7151},
	orcid-numbers = {Pankotai, Tibor/0000-0001-9810-5465}
}

@article{MTMT:34840255,
	title = {Identification of immune subsets with distinct lectin binding signatures using multi-parameter flow cytometry: correlations with disease activity in systemic lupus erythematosus},
	url = {https://m2.mtmt.hu/api/publication/34840255},
	author = {Szabó, Enikő and Faragó, Anna and Bodor, Gergely and Gémes, Nikolett and Puskás, László and Kovács, László and Szebeni, Gábor},
	doi = {10.3389/fimmu.2024.1380481},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {15},
	unique-id = {34840255},
	issn = {1664-3224},
	year = {2024},
	eissn = {1664-3224},
	orcid-numbers = {Kovács, László/0000-0003-4457-1430; Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:34790193,
	title = {Lectin-Based Immunophenotyping and Whole Proteomic Profiling of CT-26 Colon Carcinoma Murine Model},
	url = {https://m2.mtmt.hu/api/publication/34790193},
	author = {Faragó, Anna and Zvara, Ágnes and Tiszlavicz, László and Hunyadi-Gulyás Éva, Csilla and Darula, Zsuzsanna and Hegedűs, Zoltán and Szabó, Enikő and Surguta, Sára Eszter and Tóvári, József and Puskás, László and Szebeni, Gábor},
	doi = {10.3390/ijms25074022},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34790193},
	issn = {1661-6596},
	abstract = {A murine colorectal carcinoma (CRC) model was established. CT26 colon carcinoma cells were injected into BALB/c mice's spleen to study the primary tumor and the mechanisms of cell spread of colon cancer to the liver. The CRC was verified by the immunohistochemistry of Pan Cytokeratin and Vimentin expression. Immunophenotyping of leukocytes isolated from CRC-bearing BALB/c mice or healthy controls, such as CD19+ B cells, CD11+ myeloid cells, and CD3+ T cells, was carried out using fluorochrome-labeled lectins. The binding of six lectins to white blood cells, such as galectin-1 (Gal1), siglec-1 (Sig1), Sambucus nigra lectin (SNA), Aleuria aurantia lectin (AAL), Phytolacca americana lectin (PWM), and galectin-3 (Gal3), was assayed. Flow cytometric analysis of the splenocytes revealed the increased binding of SNA, and AAL to CD3 + T cells and CD11b myeloid cells; and increased siglec-1 and AAL binding to CD19 B cells of the tumor-bearing mice. The whole proteomic analysis of the established CRC-bearing liver and spleen versus healthy tissues identified differentially expressed proteins, characteristic of the primary or secondary CRC tissues. KEGG Gene Ontology bioinformatic analysis delineated the established murine CRC characteristic protein interaction networks, biological pathways, and cellular processes involved in CRC. Galectin-1 and S100A4 were identified as upregulated proteins in the primary and secondary CT26 tumor tissues, and these were previously reported to contribute to the poor prognosis of CRC patients. Modelling the development of liver colonization of CRC by the injection of CT26 cells into the spleen may facilitate the understanding of carcinogenesis in human CRC and contribute to the development of novel therapeutic strategies.},
	keywords = {colorectal carcinoma; lectin binding sugar code; proteomic analysis of murine CRC},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Tiszlavicz, László/0000-0003-1134-6587; Tóvári, József/0000-0002-5543-3204; Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:34743202,
	title = {A bipartite NLS motif mediates the nuclear import of Drosophila moesin},
	url = {https://m2.mtmt.hu/api/publication/34743202},
	author = {Kovács, Zoltán and Bajusz, Csaba and Szabó, Anikó and Borkúti, Péter and Vedelek, Balázs and Benke, Reka and Lipinszki, Zoltán and Kristó, Ildikó and Vilmos, Péter},
	doi = {10.3389/fcell.2024.1206067},
	journal-iso = {FRONT CELL DEV BIOL},
	journal = {FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY},
	volume = {12},
	unique-id = {34743202},
	issn = {2296-634X},
	abstract = {The ERM protein family, which consists of three closely related proteins in vertebrates, ezrin, radixin, and moesin (ERM), is an ancient and important group of cytoplasmic actin-binding and organizing proteins. With their FERM domain, ERMs bind various transmembrane proteins and anchor them to the actin cortex through their C-terminal F-actin binding domain, thus they are major regulators of actin dynamics in the cell. ERMs participate in many fundamental cellular processes, such as phagocytosis, microvilli formation, T-cell activation and tumor metastasis. We have previously shown that, besides its cytoplasmic activities, the single ERM protein of Drosophila melanogaster, moesin, is also present in the cell nucleus, where it participates in gene expression and mRNA export. Here we study the mechanism by which moesin enters the nucleus. We show that the nuclear import of moesin is an NLS-mediated, active process. The nuclear localization sequence of the moesin protein is an evolutionarily highly conserved, conventional bipartite motif located on the surface of the FERM domain. Our experiments also reveal that the nuclear import of moesin does not require PIP2 binding or protein activation, and occurs in monomeric form. We propose, that the balance between the phosphorylated and non-phosphorylated protein pools determines the degree of nuclear import of moesin.},
	keywords = {PHOSPHORYLATION; BINDING; LOCALIZATION; IDENTIFICATION; NUCLEUS; STRUCTURAL BASIS; DROSOPHILA; CELL BIOLOGY; ERM PROTEINS; ezrin; moesin; CYTOPLASMIC TAIL; ERM; PIP2; importin; MERLIN; LINKS ACTIN},
	year = {2024},
	eissn = {2296-634X},
	orcid-numbers = {Vedelek, Balázs/0000-0001-6981-0026; Lipinszki, Zoltán/0000-0002-2067-0832}
}

@article{MTMT:34575638,
	title = {FERM domain-containing proteins are active components of the cell nucleus},
	url = {https://m2.mtmt.hu/api/publication/34575638},
	author = {Borkúti, Péter and Kristó, Ildikó and Szabó, Anikó and Kovács, Zoltán and Vilmos, Péter},
	doi = {10.26508/lsa.202302489},
	journal-iso = {LIFE SCI ALLIANCE},
	journal = {LIFE SCIENCE ALLIANCE},
	volume = {7},
	unique-id = {34575638},
	abstract = {The FERM domain is a conserved and widespread protein module that appeared in the common ancestor of amoebae, fungi, and animals, and is therefore now found in a wide variety of species. The primary function of the FERM domain is localizing to the plasma membrane through binding lipids and proteins of the membrane; thus, for a long time, FERM domain-containing proteins (FDCPs) were considered exclusively cytoskeletal. Although their role in the cytoplasm has been extensively studied, the recent discovery of the presence and importance of cytoskeletal proteins in the nucleus suggests that FDCPs might also play an important role in nuclear function. In this review, we collected data on their nuclear localization, transport, and possible functions, which are still scattered throughout the literature, with special regard to the role of the FERM domain in these processes. With this, we would like to draw attention to the exciting, new dimension of the role of FDCPs, their nuclear activity, which could be an interesting novel direction for future research.},
	keywords = {GENE-EXPRESSION; STRUCTURAL BASIS; FOCAL ADHESION; TERMINAL DOMAIN; SUBCELLULAR-LOCALIZATION; Export signal; Ankyrin Repeat; FAK INTERACTION; KINDLIN FAMILY},
	year = {2024},
	eissn = {2575-1077}
}

@article{MTMT:34600487,
	title = {Measuring Transposable Element Activity in Adult Drosophila Ovaries},
	url = {https://m2.mtmt.hu/api/publication/34600487},
	author = {Szabó, Anikó and Borkúti, Péter and Kovács, Zoltán and Kristó, Ildikó and Abonyi, Csilla and Vilmos, Péter},
	doi = {10.1007/978-1-0716-2970-3_16},
	journal-iso = {METHODS MOL BIOL},
	journal = {METHODS IN MOLECULAR BIOLOGY},
	volume = {2626},
	unique-id = {34600487},
	issn = {1064-3745},
	year = {2023},
	eissn = {1940-6029},
	pages = {309-321}
}

@article{MTMT:34600475,
	title = {Detection of Actin in Nuclear Protein Fraction Isolated from Adult Drosophila Ovary},
	url = {https://m2.mtmt.hu/api/publication/34600475},
	author = {Kristó, Ildikó and Borkúti, Péter and Kovács, Zoltán and Szabó, Anikó and Szikora, Szilárd and Vilmos, Péter},
	doi = {10.1007/978-1-0716-2970-3_19},
	journal-iso = {METHODS MOL BIOL},
	journal = {METHODS IN MOLECULAR BIOLOGY},
	volume = {2626},
	unique-id = {34600475},
	issn = {1064-3745},
	year = {2023},
	eissn = {1940-6029},
	pages = {353-364}
}

@article{MTMT:34473960,
	title = {Monitoring the photosynthetic activity at single-cell level in Haematococcus lacustris},
	url = {https://m2.mtmt.hu/api/publication/34473960},
	author = {Patil, Priyanka Pradeep and Nagy, Krisztina and Ábrahám, Ágnes and Vass, Imre and Szabó, Milán},
	doi = {10.32615/ps.2023.042},
	journal-iso = {PHOTOSYNTHETICA},
	journal = {PHOTOSYNTHETICA},
	volume = {61},
	unique-id = {34473960},
	issn = {0300-3604},
	abstract = {Haematococcus lacustris is an important species of green algae because it produces the high-value carotenoid astaxanthin. Astaxanthin production is enhanced by various stress conditions causing the transformation of green vegetative cells to red cells with high amounts of astaxanthin, which plays various photoprotective and antioxidant roles. Although intensive research has been conducted to reveal the regulation of astaxanthin production, the photosynthetic capacity of the various cell forms is unresolved at the single-cell level. In this work, we characterized the photosynthetic and morphological changes of Haematococcus cells, using a combination of microfluidic tools and microscopic chlorophyll fluorescence imaging. We found marked but reversible changes in the variable chlorophyll fluorescence signatures upon the transformation of green cells to red cells, and we propose that the photosynthetic activity as revealed by single-cell chlorophyll fluorescence kinetics serves as a useful phenotypic marker of the different cell forms of Haematococcus.},
	keywords = {PHOTOSYNTHESIS; CHLOROPHYLL FLUORESCENCE; astaxanthin; photoprotection; cyclic electron flow; Haematococcus lacustris; GREEN-ALGA; VIVO CHLOROPHYLL FLUORESCENCE; LOW-WAVE PHENOMENON; PLUVIALIS},
	year = {2023},
	eissn = {1573-9058},
	pages = {473-482}
}