@article{MTMT:35192390, title = {In Vivo Efficacy of Rezafungin, Anidulafungin, Caspofungin, and Micafungin against Four Candida auris Clades in a Neutropenic Mouse Bloodstream Infection Model}, url = {https://m2.mtmt.hu/api/publication/35192390}, author = {Balázsi, Dávid and Tóth, Zoltán and Locke, Jeffrey B. and Borman, Andrew M. and Forgács, Lajos and Balla , Noémi and Kovács, Fruzsina and Kovács, Renátó and Amano, Chiaki and Baran, Tugba Ilay and Majoros, László}, doi = {10.3390/jof10090617}, journal-iso = {J FUNGI}, journal = {JOURNAL OF FUNGI}, volume = {10}, unique-id = {35192390}, abstract = {Objectives: Rezafungin is the first new drug approved to treat candidaemia and invasive candidiasis in more than 10 years. However, data are scant on the in vivo efficacy of rezafungin and the other three approved echinocandins against different Candida auris clades. Methods: This study involved 10 isolates representing 4 C. auris clades: South Asian (n = 2), East Asian (n = 2), South African (n = 2), and South American (n = 4, including 2 environmental isolates). In the lethality experiment and fungal tissue burden experiment (kidney, heart, and brain), cyclophosphamide-treated BALB/c male mice were intravenously infected (107 and 8 × 106 colony-forming units [CFU]/mouse, respectively). A 20 mg/kg dose of rezafungin was administered on days 1, 3, and 6. Alternatively, beginning 24 h post-infection, mice received 3 mg/kg of caspofungin, 5 mg/kg of micafungin, or 5 mg/kg of anidulafungin once daily for 6 days. Results: Regardless of isolate and clade, all echinocandin regimens improved survival after 21 days (p = 0.0041 to p < 0.0001). All echinocandins frequently produced >3-log mean CFU/g decreases in the fungal kidney and heart burdens, although some of these decreases were not statistically significant. Rezafungin, regardless of clade, produced 3–5 and 2–4 log CFU/g decreases in the kidney and heart burdens, respectively. Echinocandins did not inhibit fungal growth in the brain. Histopathological examination performed on day 7 showed no fungal cells in the heart and kidneys of rezafungin-treated mice and to a lesser extent, caspofungin-treated mice, regardless of the clinical isolate. All echinocandin-treated mice showed medium and/or large foci of fungal cells in their cerebrum or cerebellum. Conclusions: Regardless of the C. auris clade, rezafungin activity in vivo was comparable to or improved over that of the three previously approved echinocandins.}, year = {2024}, eissn = {2309-608X}, pages = {617}, orcid-numbers = {Locke, Jeffrey B./0000-0001-8145-0828; Borman, Andrew M./0000-0003-0585-5721; Kovács, Renátó/0000-0003-3946-2424} } @article{MTMT:35077128, title = {Expression of hsa-miRNA-15b, -99b, -181a and Their Relationship to Angiogenesis in Renal Cell Carcinoma}, url = {https://m2.mtmt.hu/api/publication/35077128}, author = {Király, József and Szabó, Erzsébet and Fodor, Petra and Vass, Anna and Choudhury, Mahua and Gesztelyi, Rudolf and Szász, Csaba and Flaskó, Tibor and Dobos, Nikoletta and Zsebik, Barbara and Steli, Ákos József and Halmos, Gábor and Szabó, Zsuzsanna}, doi = {10.3390/biomedicines12071441}, journal-iso = {BIOMEDICINES}, journal = {BIOMEDICINES}, volume = {12}, unique-id = {35077128}, abstract = {Background: MicroRNAs (miRNAs) play a regulatory role in various human cancers. The roles of hsa-miR-15a-5p, hsa-miR-99b-5p, and hsa-miR-181a-5p have not been fully explored in the angiogenesis of renal cell carcinoma (RCC). Aims: The present study aimed to evaluate the expression of these miRNAs in tumorous and adjacent healthy tissues of RCC. Methods: Paired tumorous and adjacent normal kidney tissues from 20 patients were studied. The expression levels of hsa-miR-15b-5p, hsa-miR-99b-5p, and hsa-miR-181a-5p were quantified by TaqMan miRNA Assays. Putative targets were analyzed by qRT-PCR. Results: Significant downregulation of all three miRNAs investigated was observed in tumorous samples compared to adjacent normal kidney tissues. Spearman analysis showed a negative correlation between the expression levels of miRNAs and the pathological grades of the patients. Increased expression of vascular endothelial growth factor-A (VEGF-A) and hypoxia-inducible factor-1α (HIF-1α), a tissue inhibitor of metalloproteinases-1 (TIMP-1), was observed in tumorous samples compared to adjacent normal tissues. Depletion of tissue inhibitors of metalloproteinase-2 (TIMP-2) and metalloproteinase-2 (MMP-2) was detected compared to normal adjacent tissues. The examined miRNAs might function as contributing factors to renal carcinogenesis. However, more prospective studies are warranted to evaluate the potential role of miRNAs in RCC angiogenesis.}, year = {2024}, eissn = {2227-9059}, pages = {1441}, orcid-numbers = {Gesztelyi, Rudolf/0000-0001-7911-055X} } @article{MTMT:34688574, title = {Comparative transcriptional analysis of Candida auris biofilms following farnesol and tyrosol treatment}, url = {https://m2.mtmt.hu/api/publication/34688574}, author = {Jakab, Ágnes and Kovács, Fruzsina and Balla , Noémi and Nagy-Köteles, Csaba and Ragyák, Ágota and Nagy, Fruzsina and Borman, Andrew and Majoros, László and Kovács, Renátó}, doi = {10.1128/spectrum.02278-23}, journal-iso = {MICROBIOL SPEC}, journal = {MICROBIOLOGY SPECTRUM}, volume = {12}, unique-id = {34688574}, issn = {2165-0497}, year = {2024}, eissn = {2165-0497} } @CONFERENCE{MTMT:34453415, title = {Comprehensive functional analyses of the bzip transciption factors AtfA and AtfB in Aspergillus Nidulans}, url = {https://m2.mtmt.hu/api/publication/34453415}, author = {Kocsis, Beatrix and Mi-Kyung, Lee and Jae-Hyuk, Yu and Nagy, Tibor and Daróczi, Lajos and Batta, Gyula Gábor (Ifj.) and Pócsi, István and Leiter, Éva Juliánna}, booktitle = {16th European Conference on Fungal Genetics: Programme & Abstracts}, unique-id = {34453415}, year = {2023}, pages = {705-706}, orcid-numbers = {Nagy, Tibor/0000-0001-8568-914X; Batta, Gyula Gábor (Ifj.)/0000-0001-8735-6920} } @CONFERENCE{MTMT:34453385, title = {Genome-wide study of AtfA/AtfB-mediated menadione stress response during asexual development in Aspergillus nidulans}, url = {https://m2.mtmt.hu/api/publication/34453385}, author = {Kocsis, Beatrix and Mi-Kyung, Lee and Antal, Károly and Jae-Hyuk, Yu and Pócsi, István and Leiter, Éva Juliánna and Emri, Tamás}, booktitle = {The 19th International Aspergillus Meeting: Asperfest}, unique-id = {34453385}, year = {2023}, pages = {31-32} } @article{MTMT:34269173, title = {Personalized Nasal Protective Devices: Importance and Perspectives}, url = {https://m2.mtmt.hu/api/publication/34269173}, author = {Quoc, Thinh To and Bácskay, Ildikó and Siposné Fehér, Pálma and Pallér, Ádám and Papp, Boglárka and Bíró, Krisztina and Ujhelyi, Zoltán}, doi = {10.3390/life13112116}, journal-iso = {LIFE-BASEL}, journal = {LIFE-BASEL}, volume = {13}, unique-id = {34269173}, abstract = {Nowadays, in addition to diseases caused by environmental pollution, the importance of personalized protection against various infectious agents has become of paramount importance. Besides medicine, several technical and technological studies have been carried out to develop suitable devices. One such revolutionary solution is the use of personalized nasal filters, which allow our body to defend itself more effectively against external environmental damage and pathogens. These filters are small devices that are placed in the nose and specifically filter the inhaled environmental contaminants, allergens, and microorganisms according to individual needs. These devices not only play a key role in maintaining our health but also contribute to environmental protection, reducing the inhalation of pollutants and their harmful impact on the natural environment. Another advantage of personalized filters is that they also provide an opportunity to strengthen our individual immune systems. The use of personalized filters allows medicine to provide optimized protection for everyone, focusing on individual genetic and immunological conditions. The momentum behind the development and research of personalized nasal filters has reached astonishing proportions today. Nowadays, many research groups and medical institutions are working to create new materials, nanotechnologies, and bioinformatics solutions in order to create even more effective personalized nasal filters that can also be shaped easily and safely. Considering the needs of the users is at least as important during development as the efficiency of the device. These two properties together determine the success of the product. Industry research focuses not only on improving the efficiency of devices, but also on making them more responsive to user needs, comfort, and portability. Based on all this, it can be concluded that personalized nasal filters can be a promising and innovative solution for protection against environmental pollutants and pathogens. Through a commitment to the research and development of technology, the long-term impact of such devices on our health and the environment can be significant, contributing to improving people’s quality of life and creating a sustainable future. With unique solutions and continuous research, we give hope that in the future, despite the environmental challenges, we can enjoy the protection of our health with even more efficient and sophisticated devices.}, year = {2023}, eissn = {2075-1729}, pages = {2116} } @article{MTMT:34201609, title = {Carbapenem-resistant Escherichia coli in Black-headed gulls, the Danube, and human clinical samples: A One Health comparison of contemporary isolates}, url = {https://m2.mtmt.hu/api/publication/34201609}, author = {Nagy, József Bálint and Koleszár, Balázs and Khayer, Bernadett and Róka, Eszter and Laczkó, Levente and Ungvári, Erika and Kaszab, Eszter and Bali, Krisztina and Bányai, Krisztián and Vargha, Márta and Lovas-Kiss, Ádám and Tóth, Ákos and Kardos, Gábor}, doi = {10.1016/j.jgar.2023.10.002}, journal-iso = {J GLOB ANTIMICROB RE}, journal = {JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE}, volume = {35}, unique-id = {34201609}, issn = {2213-7165}, year = {2023}, eissn = {2213-7173}, pages = {257-261}, orcid-numbers = {Khayer, Bernadett/0000-0003-3484-9232} } @article{MTMT:34081680, title = {Antifungal Activity of Glucosinolate-Derived Nitriles and Their Synergistic Activity with Glucosinolate-Derived Isothiocyanates Distinguishes Various Taxa of Brassicaceae Endophytes and Soil Fungi}, url = {https://m2.mtmt.hu/api/publication/34081680}, author = {Szűcs, Zsolt and Plaszkó, Tamás and Bódor, Eszter and Csoma, Hajnalka and Ács-Szabó, Lajos and Kiss, Attila and Vasas, Gábor and Gonda, Sándor}, doi = {10.3390/plants12142741}, journal-iso = {PLANTS-BASEL}, journal = {PLANTS-BASEL}, volume = {12}, unique-id = {34081680}, abstract = {The glucosinolates of Brassicaceae plants are converted into bioactive isothiocyanates and other volatiles during a challenge by pathogens and other biotic stressors. However, the role of alternative downstream products with weaker potency (e.g., nitriles) is far from being fully understood. This study tested the possible synergistic antifungal interaction between various glucosinolate-derived nitriles and 2-phenylethyl isothiocyanate (PEITC) on 45 fungal strains, including endophytes from horseradish roots (Brassicaceae) and soil fungi, using an airtight system enabling the accurate study of extremely volatile antifungal agents. The median minimal inhibitory concentrations (MICs) were 1.28, 6.10, 27.00 and 49.72 mM for 1H-indole-3-acetonitrile (IAN), 3-phenylpropanenitrile (PPN), 4-(methylsulfanyl)-butanenitrile (MSBN) and 3-butenenitrile (BN, = allyl cyanide), respectively. Thus, nitriles were considerably weaker antifungal agents compared to PEITC with a median MIC of 0.04 mM. For the same nitriles, the median fractional inhibitory concentration indices (FICIs) of the combinations were 0.562, 0.531, 0.562 and 0.625, respectively. Altogether, 47.7%, 56.8%, 50.0% and 27.3% of tested fungal strains showed a synergistic antifungal activity (FICI ≤ 0.5) for the nitrile–isothiocyanate combinations, respectively. Hypocreales strains showed the least sensitivity towards the GSL decomposition products and their combinations. The mean MIC values for PEITC showed 0.0679 ± 0.0358, 0.0400 ± 0.0214, 0.0319 ± 0.0087 and 0.0178 ± 0.0171 mM for Hypocreales, Eurotiales, Glomerellales and Pleosporales, respectively. In addition, nitriles, especially IAN, also showed significant differences. For the same fungi, the median FICI values fell in the ranges of 0.61–0.67, 0.52–0.61, 0.40–0.50 and 0.48–0.67, respectively, depending on the nitrile. Our results suggest that glucosinolate-derived nitriles may enhance isothiocyanate antifungal activity and that they may play an active role in shaping the plant microbiome and contribute to the filtering of microbes by plants.}, keywords = {ISOTHIOCYANATE; Antifungal activity; nitrile; ITC; Synergy; Soil fungi; Endophyte fungi}, year = {2023}, eissn = {2223-7747}, orcid-numbers = {Plaszkó, Tamás/0000-0003-4211-3823; Kiss, Attila/0000-0003-3601-5143; Gonda, Sándor/0000-0001-9776-0249} } @article{MTMT:34008857, title = {Formulation and characterization of pramipexole containing buccal films for using in Parkinson's disease}, url = {https://m2.mtmt.hu/api/publication/34008857}, author = {Pamlényi, Krisztián and Regdon, Géza (ifj.) and Jójártné Laczkovich, Orsolya and Nemes, Dániel and Bácskay, Ildikó and Kristó, Katalin}, doi = {10.1016/j.ejps.2023.106491}, journal-iso = {EUR J PHARM SCI}, journal = {EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES}, volume = {187}, unique-id = {34008857}, issn = {0928-0987}, year = {2023}, eissn = {1879-0720}, orcid-numbers = {Regdon, Géza (ifj.)/0000-0002-6921-3069; Nemes, Dániel/0000-0002-5477-0540} } @article{MTMT:33823864, title = {Glaukóma : a látás "néma tolvaja"}, url = {https://m2.mtmt.hu/api/publication/33823864}, author = {Józsa, Liza}, journal-iso = {GYÓGYSZERÉSZET}, journal = {GYÓGYSZERÉSZET}, volume = {3}, unique-id = {33823864}, issn = {0017-6036}, year = {2023}, pages = {1-5} }