TY - JOUR AU - Gödöny, Krisztina AU - Herczeg, Róbert AU - Gyenesei, Attila AU - Várnagy, Ákos AU - Bognár, Zoltán AU - Kovács, L. Gábor AU - Szekeres, Júlia AU - Mauchart, Péter AU - Nagy, Bernadett AU - Erostyák, János AU - Kovács, Kálmán András AU - Bódis, József TI - Clinical Benefits of Decreased Photo-Oxidative Stress on Human Embryo Development JF - MEDICAL PRINCIPLES AND PRACTICE J2 - MED PRIN PRACT PY - 2024 SN - 1011-7571 DO - 10.1159/000536358 UR - https://m2.mtmt.hu/api/publication/34763514 ID - 34763514 N1 - Funding Agency and Grant Number: Recovery and Resilience Facility of the European Union within the framework of Programme Szechenyi Plan Plus [RRF-2.3.1-21-2022-00012]; HUN-REN Hungarian Research Network; ELIXIR Hungary; National Research, Development, and Innovation Fund of Hungary [TKP2021-EGA-17]; [GINOP-2.3.4-15-2020-00010]; [GINOP-2.3.1-20-2020-00001]; [ERASMUS+-2019-0-HU01-KA203-061251] Funding text: Project No. RRF-2.3.1-21-2022-00012, titled National Laboratoryon Human Reproduction, has been implemented with the support provided by the Recovery and Resilience Facility of the European Union within the framework of Programme Szechenyi Plan Plus, andthis project has received funding from the HUN-REN HungarianResearch Network. The research was performed in collaboration withthe Genomics and Bioinformatics Core Facility at the SzentagothaiResearch Centre of the University of Pecs. R.H and A.G. weresupported by the grants GINOP-2.3.4-15-2020-00010, GINOP-2.3.1-20-2020-00001, and ERASMUS+-2019-0-HU01-KA203-061251.Bioinformatics infrastructurewas supported by ELIXIR Hungary(http://elixir-hungary.org). J.E. was supported by TKP2021-EGA-17provided by the National Research, Development, and Innovation Fund of Hungary,financed under the EGA 17 funding scheme. AB - Objective: Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Materials and Methods: We tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control. Results: Blastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate. Conclusions: The implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety. LA - English DB - MTMT ER - TY - JOUR AU - Ahmadi, Hamid AU - Soltani-Zangbar, Mohammad Sadegh AU - Yousefi, Mehdi AU - Baradaran, Behzad AU - Bromand, Saro AU - Aghebati-Maleki, Leili AU - Szekeres, Júlia TI - The evaluation of PD-1 and Tim-3 expression besides their related miRNAs in PBMCs of women with recurrent pregnancy loss JF - IMMUNOLOGY LETTERS J2 - IMMUNOL LETT VL - 266 PY - 2024 PG - 9 SN - 0165-2478 DO - 10.1016/j.imlet.2024.106837 UR - https://m2.mtmt.hu/api/publication/34541561 ID - 34541561 AB - Recurrent pregnancy loss (RPL) is a multifactorial disorder, associated with immunologic abnormalities. During pregnancy, the maternal immune system uses different tolerance mechanisms to deal with a semi-allogenic fetus. The expression of immune checkpoints and their related miRNAs in immune cells can ensure pregnancy at the feto-maternal interface by modulating immune responses. This study aims to evaluate the expression of the immune checkpoint molecules PD-1 and Tim-3 on circulating T cells by flow cytometry, that of mir-138 and mir-155 in PBMCs by Real-time PCR, and the concentrations of TGF-β and IP-10 in the sera of women suffering from RPL as well as of gestational age-matched healthy pregnant women by ELISA. The percentage of PD-1 or Tim-3 expressing CD8+ T cells was significantly lower in RPL patients compared to the controls, while there was no significant difference in Tim-3 expression of CD4+ T cells between the two groups. The mRNA of both the PD-1 and Tim-3 genes were downregulated in PBMCs of RPL patients compared to controls, however, the the difference was not statistically significant for Tim-3. The concentration of TGF-β was significantly lower and that of IP-10 was significantly higher in the sera of RPL patients than in those of the controls. The relative expression of mir-138 and miR-155 were significantly lower, in PBMCs of RPL patients than in those of healthy pregnant women. These data confirm that by affecting cytokine production, immune checkpoints, and microRNAs play a role in establishing the appropriate local immune environment for successful pregnancy. The wider analysis of immune checkpoints may also yield new biomarkers for the diagnosis and prevention of RPL. LA - English DB - MTMT ER - TY - JOUR AU - Bognar, Zoltan AU - Csabai-Tanics, Timea J. AU - Gorgey, Eva AU - Miko, Eva AU - Szekeres, Júlia TI - The effect of Calcitriol on the development and implantation capacity of embryos from hyper-stimulated mice JF - JOURNAL OF REPRODUCTIVE IMMUNOLOGY J2 - J REPROD IMMUNOL VL - 159 PY - 2023 SP - 27 EP - 27 PG - 1 SN - 0165-0378 DO - 10.1016/j.jri.2023.104060 UR - https://m2.mtmt.hu/api/publication/34214976 ID - 34214976 LA - English DB - MTMT ER - TY - JOUR AU - Bognar, Zoltan AU - Csabai-Tanics, Timea Judith AU - Gorgey, Eva AU - Szekeres, Júlia TI - The effect of Calcitriol on oocyte and embryo development mice JF - JOURNAL OF REPRODUCTIVE IMMUNOLOGY J2 - J REPROD IMMUNOL VL - 158 PY - 2023 PG - 1 SN - 0165-0378 DO - 10.1016/j.jri.2022.103612 UR - https://m2.mtmt.hu/api/publication/34212243 ID - 34212243 N1 - WoS:hiba:001061855500105 2023-12-31 23:29 cikkazonosító nem egyezik LA - English DB - MTMT ER - TY - JOUR AU - Szekeres, Júlia AU - Tanics, Timea Judith Csabai AU - Gorgey, Eva AU - Bognar, Zoltan AU - Mikó, Éva TI - [8h30-50] Foetal-maternal interactions in pregnancy JF - JOURNAL OF REPRODUCTIVE IMMUNOLOGY J2 - J REPROD IMMUNOL VL - 158 PY - 2023 PG - 1 SN - 0165-0378 DO - 10.1016/j.jri.2022.103517 UR - https://m2.mtmt.hu/api/publication/34212242 ID - 34212242 N1 - WoS:hiba:001061855500002 2023-12-31 23:29 cikkazonosító nem egyezik LA - English DB - MTMT ER - TY - JOUR AU - Gorgey, Eva AU - Tanics, Timea Judith Csabai AU - Bognar, Zoltan AU - Mikó, Éva AU - Szekeres, Júlia TI - [9h20-9h30] Immune checkpoint ligands expressed by embryo-derived extracellular vesicles JF - JOURNAL OF REPRODUCTIVE IMMUNOLOGY J2 - J REPROD IMMUNOL VL - 158 PY - 2023 PG - 1 SN - 0165-0378 DO - 10.1016/j.jri.2022.103520 UR - https://m2.mtmt.hu/api/publication/34212237 ID - 34212237 N1 - WoS:hiba:001061855500005 2023-12-31 23:29 cím nem egyezik LA - English DB - MTMT ER - TY - JOUR AU - Ahmadi, Hamid AU - Aghebati-Maleki, Leili AU - Rashidiani, Shima AU - Csabai-Tanics, Tímea Judith AU - Nnaemeka, Obodo B. AU - Szekeres, Júlia TI - Long-Term Effects of ART on the Health of the Offspring JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 17 PG - 27 SN - 1661-6596 DO - 10.3390/ijms241713564 UR - https://m2.mtmt.hu/api/publication/34124223 ID - 34124223 N1 - ISSN:1422-0067 AB - Assisted reproductive technologies (ART) significantly increase the chance of successful pregnancy and live birth in infertile couples. The different procedures for ART, including in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), intrauterine insemination (IUI), and gamete intrafallopian tube transfer (GIFT), are widely used to overcome infertility-related problems. In spite of its inarguable usefulness, concerns about the health consequences of ART-conceived babies have been raised. There are reports about the association of ART with birth defects and health complications, e.g., malignancies, high blood pressure, generalized vascular functional disorders, asthma and metabolic disorders in later life. It has been suggested that hormonal treatment of the mother, and the artificial environment during the manipulation of gametes and embryos may cause genomic and epigenetic alterations and subsequent complications in the health status of ART-conceived babies. In the current study, we aimed to review the possible long-term consequences of different ART procedures on the subsequent health status of ART-conceived offspring, considering the confounding factors that might account for/contribute to the long-term consequences. LA - English DB - MTMT ER - TY - JOUR AU - Bognár, Zoltán AU - Csabai-Tanics, Tímea Judith AU - Görgey, Éva AU - Mikó, Éva AU - Horváth-Szalai, Zoltán AU - Szekeres, Júlia TI - The effect of calcitriol on the development and implantation capacity of embryos from hyper-stimulated mice JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 14 PY - 2023 PG - 8 SN - 1664-3224 DO - 10.3389/fimmu.2023.1200704 UR - https://m2.mtmt.hu/api/publication/34104633 ID - 34104633 AB - The evidence concerning the role of vitamin D (VD) in reproduction is still inconclusive. Calcitriol was given to superovulated female mice at the time of FSH injection (Group A), or at day 0.5 of pregnancy (Group B). The retrieved and cultured embryos were transferred to the uteri of pseudopregnant females. Ten animals from each group conceived naturally, and at day 7.5 of pregnancy, the implantation sites were counted. Serum hormone concentrations were determined by ELISA. The expression of CD70, PD-L1, OX-40L, and PIBF on extracellular vesicles (EVs) was tested by flow cytometry. Calcitriol treatment did not alter serum oestradiol concentrations, while 25(OH) D levels significantly decreased in both treated groups. Progesterone concentrations were significantly higher in group A and lower in group B than in the controls. On EVs produced by group B embryos PIBF, CD70, and OX-40L expression were significantly lower, while that of PD-L1 was significantly higher than that of controls. Calcitriol treatment decreased the fertilization rate in group A, and the blastulation rate of cultured embryos in group B, while the implantation capacity of the embryos was not affected, suggesting that depending on the time of administration, VD has an adverse effect on oocyte maturation and embryo development, but not on the implantation rates. LA - English DB - MTMT ER - TY - JOUR AU - Mikó, Éva AU - Barakonyi, Alíz TI - The Role of Hydrogen-Peroxide (H2O2) Produced by Vaginal Microbiota in Female Reproductive Health JF - ANTIOXIDANTS J2 - ANTIOXIDANTS-BASEL VL - 12 PY - 2023 IS - 5 PG - 17 SN - 2076-3921 DO - 10.3390/antiox12051055 UR - https://m2.mtmt.hu/api/publication/33937657 ID - 33937657 AB - Female reproductive health is strongly associated with healthy vaginal microbiota, which is thought to be ensured by the dominance of certain Lactobacillus species. Lactobacilli control the vaginal microenvironment through several factors and mechanisms. One of them is their ability to produce hydrogen peroxide (H2O2). The role of Lactobacillus-derived H2O2 in the vaginal microbial community has been intensively investigated in several studies with many designs. However, results and data are controversial and challenging to interpret in vivo. Defining the underlying mechanisms responsible for a physiological vaginal ecosystem is crucial since it could directly affect probiotic treatment attempts. This review aims to summarize current knowledge on the topic, focusing on probiotic treatment possibilities. LA - English DB - MTMT ER - TY - JOUR AU - Meggyes, Mátyás AU - Feik, Timoteus AU - Nagy, Dávid AU - Polgár, Beáta AU - Szereday, László TI - CD8 and CD4 Positive NKT Subpopulations and Immune-Checkpoint Pathways in Early-Onset Preeclampsia and Healthy Pregnancy JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 2 PG - 16 SN - 1661-6596 DO - 10.3390/ijms24021390 UR - https://m2.mtmt.hu/api/publication/33559387 ID - 33559387 N1 - Department of Medical Microbiology and Immunology, Medical School, University of Pecs, 12 Szigeti Street, Pecs, 7624, Hungary Janos Szentagothai Research Centre, 20 Ifjusag Street, Pecs, 7624, Hungary Institute of Geobotany/Plant Ecology, Martin-Luther-University, Große Steinstraße 79/80, Halle (Saale), 06108, Germany Export Date: 2 October 2023 Correspondence Address: Meggyes, M.; Department of Medical Microbiology and Immunology, 12 Szigeti Street, Hungary; email: meggyes.matyas@pte.hu AB - Although many studies have investigated the clinical aspect of early-onset preeclampsia, our knowledge about the immunological consequences of improper placenta development is scarce. The maternal immunotolerance against the fetus is greatly influenced by the Th1 predominance developed by the mother’s immune system. Thirty-two early-onset preeclamptic and fifty-one healthy pregnant women with appropriately matched gestational age were involved in our study. Mononuclear cells were separated from peripheral venous blood and the frequency of CD8⁺, CD4⁺, double positive (DP), and double negative (DN) NKT cell subpopulations was determined using multicolor flow cytometry. Following the characterization, the expression levels of different immune checkpoint receptors and ligands were also defined. Soluble CD226 levels were quantified by ELISA. Novel and significant differences were revealed among the ratios of the investigated NKT subsets and in the expression patterns of PD-1, LAG-3, TIGIT and CD226 receptors. Further differences were determined in the expression of CD112, PD-1, LAG-3 and CD226 MFI values between the early-onset preeclamptic and the healthy pregnant groups. Our results suggest that the investigated NKT subpopulations act differently in the altered immune condition characteristic of early-onset preeclampsia and indicate that the different subsets may contribute to the compensation or maintenance of Th1 predominance. LA - English DB - MTMT ER -