TY - JOUR AU - Zhang, Xiaorong AU - Yarman, Aysu AU - Kovács, Norbert AU - Bognár, Zsófia AU - Gyurcsányi, Ervin Róbert AU - Bier, Frank F. AU - Scheller, Frieder W. TI - Specific features of epitope-MIPs and whole-protein MIPs as illustrated for AFP and RBD of SARS-CoV-2 JF - MICROCHIMICA ACTA J2 - MICROCHIM ACTA VL - 191 PY - 2024 IS - 5 PG - 9 SN - 0026-3672 DO - 10.1007/s00604-024-06325-0 UR - https://m2.mtmt.hu/api/publication/34785838 ID - 34785838 N1 - Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht Str. 24-25, Potsdam, 14476, Germany Molecular Biotechnology, Faculty of Science, Turkish-German University, Sahinkaya Cad. Beykoz, Istanbul, 34820, Turkey BME “Lendület” Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, 1111, Hungary HUN-REN-BME Computation Driven Chemistry Research Group, Műegyetem rkp. 3, Budapest, 1111, Hungary Export Date: 12 April 2024 CODEN: MIACA Correspondence Address: Zhang, X.; Institute of Biochemistry and Biology, Karl-Liebknecht Str. 24-25, Germany; email: xiaorong.zhang.1@uni-potsdam.de AB - Molecularly imprinted polymer (MIP) nanofilms for alpha-fetoprotein (AFP) and the receptor binding domain (RBD) of the spike protein of SARS-CoV-2 using either a peptide (epitope-MIP) or the whole protein (protein-MIP) as the template were prepared by electropolymerization of scopoletin. Conducting atomic force microscopy revealed after template removal and electrochemical deposition of gold a larger surface density of imprinted cavities for the epitope-imprinted polymers than when using the whole protein as template. However, comparable affinities towards the respective target protein (AFP and RBD) were obtained for both types of MIPs as expressed by the KD values in the lower nanomolar range. On the other hand, while the cross reactivity of both protein-MIPs towards human serum albumin (HSA) amounts to around 50% in the saturation region, the nonspecific binding to the respective epitope-MIPs is as low as that for the non-imprinted polymer (NIP). This effect might be caused by the different sizes of the imprinted cavities. Thus, in addition to the lower costs the reduced nonspecific binding is an advantage of epitope-imprinted polymers for the recognition of proteins. Graphical Abstract: (Figure presented.) © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024. LA - English DB - MTMT ER - TY - JOUR AU - Ahmed, Marwa AU - Hessz, Dóra AU - Gyarmati, Benjámin Sándor AU - Pancsics, Mirko AU - Kovács, Norbert AU - Gyurcsányi, Ervin Róbert AU - Kubinyi, Miklós AU - Horváth, Viola TI - A generic approach based on long-lifetime fluorophores for the assessment of protein binding to polymer nanoparticles by fluorescence anisotropy JF - NANOSCALE J2 - NANOSCALE VL - 16 PY - 2024 IS - 7 SP - 3659 EP - 3667 PG - 9 SN - 2040-3364 DO - 10.1039/d3nr02460a UR - https://m2.mtmt.hu/api/publication/34569163 ID - 34569163 N1 - Funding Agency and Grant Number: Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund under the TKP2021 funding scheme; National Research, Development and Innovation (NRDI) Office, Hungary [BME-EGA-02]; Hungarian Academy of Sciences; Stipendium Hungaricum Scholarship; [FK 138029] Funding text: The research reported in this paper is part of project no. BME-EGA-02, implemented with the support provided by the Ministry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021 funding scheme. Further support was provided by the National Research, Development and Innovation (NRDI) Office, Hungary via grant FK 138029. B. Gy. and M. A. A. acknowledge the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences and the Stipendium Hungaricum Scholarship, respectively. AB - Quantitation of protein-nanoparticle interactions is essential for the investigation of the protein corona around NPs in vivo and when using synthetic polymer nanoparticles as affinity reagents for selective protein recognition in vitro. Here, a method based on steady-state fluorescence anisotropy measurement is presented as a novel, separation-free tool for the assessment of protein-nanoparticle interactions. For this purpose, a long-lifetime luminescent Ru-complex is used for protein labelling, which exhibits low anisotropy when conjugated to the protein but displays high anisotropy when the proteins are bound to the much larger polymer nanoparticles. As a proof of concept, the interaction of lysozyme with poly(N-isopropylacrylamide-co-N-tert-butylacrylamide-co-acrylic acid) nanoparticles is studied, and fluorescence anisotropy measurements are used to establish the binding kinetics, binding isotherm and a competitive binding assay. Using long-lifetime fluorophores as protein labels, protein-nanoparticle interactions can be monitored through anisotropy change. Besides gaining thermodynamic and kinetic information on the binding process, competitive protein assays can be set up. LA - English DB - MTMT ER - TY - JOUR AU - Bognár, Zsófia AU - Supala, Eszter AU - Yarman, Aysu AU - Zhang, Xiaorong AU - Bier, Frank F. AU - Scheller, Frieder W. AU - Gyurcsányi, Ervin Róbert TI - Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein (vol 13, pg 1263, 2022) JF - CHEMICAL SCIENCE J2 - CHEM SCI VL - 14 PY - 2023 IS - 36 SP - 9980 EP - 9980 PG - 1 SN - 2041-6520 DO - 10.1039/d3sc90170j UR - https://m2.mtmt.hu/api/publication/34192911 ID - 34192911 N1 - Funding Agency and Grant Number: National Research, Development and Innovation Fund; [TKP2021] Funding text: The research reported in this paper is part of Project BME-EGA-02, implemented with support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021 funding scheme. AB - Correction for 'Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein' by Zsofia Bognar et al., Chem. Sci., 2022, 13, 1263-1269, https://doi.org/10.1039/D1SC04502D. LA - English DB - MTMT ER - TY - JOUR AU - Solymosi, Gergely Tamás AU - Gyurcsányi, Ervin Róbert TI - Understanding the potentiometric response of cation-permselective hydrophilic nanopore-based electrodes in the low ion concentration range JF - ELECTROCHEMISTRY COMMUNICATIONS J2 - ELECTROCHEM COMMUN VL - 153 PY - 2023 SN - 1388-2481 DO - 10.1016/j.elecom.2023.107540 UR - https://m2.mtmt.hu/api/publication/34085469 ID - 34085469 N1 - Export Date: 3 August 2023 CODEN: ECCMF Correspondence Address: Gyurcsányi, R.E.; BME Lendület Chemical Nanosensors Research Group, Műegyetem rkp. 3, H-1111, Hungary; email: robert.gyurcsanyi@vbk.bme.hu LA - English DB - MTMT ER - TY - JOUR AU - Szalay, Péter ED - Gyurcsányi, Ervin Róbert / Interviewed person TI - A kémiai érzékelés határainak feszegetése JF - MAGYAR KÉMIKUSOK LAPJA J2 - MAGY KEM LAP VL - 78 PY - 2023 IS - 4 SP - 108 EP - 110 PG - 3 SN - 0025-0163 UR - https://m2.mtmt.hu/api/publication/34069356 ID - 34069356 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Bognár, Zsófia AU - Kozma, József AU - Kovács, Norbert AU - Gyurcsányi, Ervin Róbert TI - Novel functional monomer for the electrochemical synthesis of highly affine epitope-imprinted polymers JF - ELECTROANALYSIS J2 - ELECTROANAL VL - 35 PY - 2023 IS - 6 SN - 1040-0397 DO - 10.1002/elan.202300025 UR - https://m2.mtmt.hu/api/publication/33727389 ID - 33727389 N1 - Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary BME Lendület Chemical Nanosensors Research Group, Műegyetem rkp. 3, Budapest, H-1111, Hungary ELKH-BME Computation Driven Chemistry Research Group, Műegyetem rkp. 3, Budapest, H-1111, Hungary CODEN: ELANE Correspondence Address: Gyurcsányi, R.E.; Department of Inorganic and Analytical Chemistry, Műegyetem rkp. 3, Hungary; email: gyurcsanyi.robert@vbk.bme.hu LA - English DB - MTMT ER - TY - JOUR AU - Kozma, József AU - Papp, Soma AU - Gyurcsányi, Ervin Róbert TI - Highly hydrophobic TEMPO-functionalized conducting copolymers for solid-contact ion-selective electrodes JF - BIOELECTROCHEMISTRY J2 - BIOELECTROCHEMISTRY VL - 150 PY - 2023 SN - 1567-5394 DO - 10.1016/j.bioelechem.2022.108352 UR - https://m2.mtmt.hu/api/publication/33453038 ID - 33453038 N1 - Funding Agency and Grant Number: Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund [BME-NVA-02, BME-EGA-02]; New National Excellence Program of the Ministry for Innovation and Technology [UNKP-21-3] Funding text: The research reported in this paper is part of project no. BME-EGA-02 and BME-NVA-02, implemented with the support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021 funding scheme. Further support was received from National Research, Development and Innovation Fund through UNKP-21-3 New National Excellence Program of the Ministry for Innovation and Technology. The authors would like to thank to Dr. Lajos Hofler for the inspiring discussion on the margin of GITT experiments. AB - Solid-contact ion-selective electrodes (SCISEs) emerged as the best electrode embodiment for miniaturized, wearable and disposable sensors for ion/electrolyte measurements in body fluids. The commercialization of inexpensive single-use "calibration-free" electrodes requires large scale manufacturing of electrodes with reproducible calibration parameters, e.g. E0. This is perhaps the most important shortcoming of SCISEs, beside the many advantages over their conventional liquid-contact counterparts. However, adjusting the E0 value for optimal potential stability is challenging for all state-of-the-art solid-contact materials, which may combine several types of transducing mechanism (e.g. capacitive and redox materials or their combination) for enhanced potential stability and analytical performance. Therefore, here we introduce for the first time the galvanostatic intermittent titration technique (GITT) to determine the best preadjusment potential. The proof of concept is shown for a novel type of solid-contact based on the copolymerization of 3,4-ethylenedioxythiophene with perfluorinated alkyl side chain (EDOTF) and (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl modified 3,4-ethylenedioxythiophene (EDOT-TEMPO). Such materials that are compliant with local electrodeposition and provide multiple functionalities, i.e. high hydrophobicity by the perfluorinated alkyl side chain, electron-to-ion transduction by the conducting polymer (EDOT) backbone and the confinement of well-defined redox couple (TEMPO), are expected to prevail as solid-contacts. LA - English DB - MTMT ER - TY - JOUR AU - Zhang, Xiaorong AU - Waffo, Armel T. AU - Yarman, Aysu AU - Kovács, Norbert AU - Bognár, Zsófia AU - Wollenberger, Ulla AU - El-Sherbiny, Ibrahim M. AU - Hassan, Rabeay Y. A. AU - Bier, Frank F. AU - Gyurcsányi, Ervin Róbert AU - Zebger, Ingo AU - Scheller, Frieder W. TI - How an ACE2 mimicking epitope-MIP nanofilm recognizes template-related peptides and the receptor binding domain of SARS-CoV-2 JF - NANOSCALE J2 - NANOSCALE VL - 14 PY - 2022 IS - 48 SP - 18106 EP - 18114 PG - 9 SN - 2040-3364 DO - 10.1039/D2NR03898F UR - https://m2.mtmt.hu/api/publication/33452970 ID - 33452970 N1 - Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht Str. 24-25, Potsdam, 14476, Germany Institut für Chemie, PC 14 Technische Universität Berlin, Straße des 17. Juni 135, Berlin, 10623, Germany Molecular Biotechnology, Faculty of Science, Turkish-German University, Sahinkaya Cad, 86, Beykoz, Istanbul, 34820, Turkey Department of Inorganic and Analytical Chemistry, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3, Budapest, H-1111, Hungary ELKH-BME Computation Driven Chemistry Research Group, Műegyetem rkp. 3, Budapest, H-1111, Hungary Nanoscience Program, University of Science and Technology (UST), Center for Materials Science (CMS), Zewail City of Science and Technology, Giza, 12578, Egypt Cited By :1 Export Date: 5 April 2023 Correspondence Address: Gyurcsányi, R.E.; Department of Inorganic and Analytical Chemistry, Műegyetem rkp. 3, Hungary; email: gyurcsanyi.robert@vbk.bme.hu Correspondence Address: Scheller, F.W.; Institute of Biochemistry and Biology, Karl-Liebknecht Str. 24-25, Germany; email: fschell@uni-potsdam.de AB - We developed original methods to confirm the liberation of the imprinted binding cavities by electrochemical template removal and identified the amino acid motif of the template which is determinant for the affinity of the epitope-imprinted polymer. LA - English DB - MTMT ER - TY - JOUR AU - Kozma, József AU - Papp, Soma AU - Gyurcsányi, Ervin Róbert TI - TEMPO-Functionalized Carbon Nanotubes for Solid-Contact Ion-Selective Electrodes with Largely Improved Potential Reproducibility and Stability JF - ANALYTICAL CHEMISTRY J2 - ANAL CHEM VL - 94 PY - 2022 IS - 23 SP - 8249 EP - 8257 PG - 9 SN - 0003-2700 DO - 10.1021/acs.analchem.2c00395 UR - https://m2.mtmt.hu/api/publication/32897099 ID - 32897099 N1 - Export Date: 21 June 2022 CODEN: ANCHA Correspondence Address: Gyurcsányi, R.E.; Department of Inorganic and Analytical Chemistry, Müegyetem rkp. 3, Hungary; email: gyurcsanyi.robert@vbk.bme.hu LA - English DB - MTMT ER - TY - JOUR AU - Bognár, Zsófia AU - de, Jonge Marien I AU - Gyurcsányi, Ervin Róbert TI - In situ silver nanoparticle coating of virions for quantification at single virus level JF - NANOSCALE J2 - NANOSCALE VL - 14 PY - 2022 IS - 6 SP - 2296 EP - 2303 PG - 8 SN - 2040-3364 DO - 10.1039/d1nr07607h UR - https://m2.mtmt.hu/api/publication/32761581 ID - 32761581 N1 - Funding Agency and Grant Number: National Research, Development, and Innovation Fund of Hungary [TKP2021-EGA-02] Funding text: This research was funded by the National Research, Development, and Innovation Fund of Hungary under Grant TKP2021-EGA-02. Soma Papp is greatly acknowledged for assisting with resistive pulse sensing measurement. The authors are grateful to Dr Peter Furjes and Levente Illes for the scanning electron microscopy measurements. We thank Marc Eleveld for the production of inactivated influenza A (H3N2) virus. AB - In situ labelling and encapsulation of biological entities, such as of single viruses, may provide a versatile approach to modulate their functionality and facilitate their detection at single particle level. Here, we introduce a novel virus metallization approach based on in situ coating of viruses in solution with silver nanoparticles (AgNP) in a two-step synthetic process, i.e. surface activation with a tannic acid - Sn(ii) coordination complex, which subsequently induces silver ion (i) reduction. The metalic coating on the virus surface opens the opportunity for electrochemical quantification of the AgNP-tagged viruses by nano-impact electrochemistry on a microelectrode with single particle sensitivity, i.e. enable the detection of particles oherwise undetectable. We show that the silver coating of the virus particles impacting the electrode can be oxidized to produce distinct current peaks the frequency of which show a linear correlation with the virus count. The proof of the concept was done with inactivated Influenza A (H3N2) viruses resulting in their quantitation down to the femtomolar concentrations (ca. 5 x 10(7) particles per mL) using 50 s counting sequences. LA - English DB - MTMT ER -