@article{MTMT:34785838,
	title = {Specific features of epitope-MIPs and whole-protein MIPs as illustrated for AFP and RBD of SARS-CoV-2},
	url = {https://m2.mtmt.hu/api/publication/34785838},
	author = {Zhang, Xiaorong and Yarman, Aysu and Kovács, Norbert and Bognár, Zsófia and Gyurcsányi, Ervin Róbert and Bier, Frank F. and Scheller, Frieder W.},
	doi = {10.1007/s00604-024-06325-0},
	journal-iso = {MICROCHIM ACTA},
	journal = {MICROCHIMICA ACTA},
	volume = {191},
	unique-id = {34785838},
	issn = {0026-3672},
	abstract = {Molecularly imprinted polymer (MIP) nanofilms for alpha-fetoprotein (AFP) and the receptor binding domain (RBD) of the spike protein of SARS-CoV-2 using either a peptide (epitope-MIP) or the whole protein (protein-MIP) as the template were prepared by electropolymerization of scopoletin. Conducting atomic force microscopy revealed after template removal and electrochemical deposition of gold a larger surface density of imprinted cavities for the epitope-imprinted polymers than when using the whole protein as template. However, comparable affinities towards the respective target protein (AFP and RBD) were obtained for both types of MIPs as expressed by the KD values in the lower nanomolar range. On the other hand, while the cross reactivity of both protein-MIPs towards human serum albumin (HSA) amounts to around 50% in the saturation region, the nonspecific binding to the respective epitope-MIPs is as low as that for the non-imprinted polymer (NIP). This effect might be caused by the different sizes of the imprinted cavities. Thus, in addition to the lower costs the reduced nonspecific binding is an advantage of epitope-imprinted polymers for the recognition of proteins. Graphical Abstract: (Figure presented.) © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024.},
	keywords = {PROTEINS; REDUCTION; DISEASES; Epitopes; alpha fetoprotein; molecularly imprinted polymers; molecularly imprinted polymer; electropolymerization; Electrochemical deposition; CORONAVIRUS; ALPHA-FETOPROTEIN; Non-specific binding; spike protein; alpha-fetoprotein (AFP); IMPRINTED POLYMERS; Receptor-binding domains; Receptor binding domain (RBD) of SARS-CoV-2; Imprinted cavities; Polymer nanofilms; Receptor binding domain of SARS-CoV-2},
	year = {2024},
	eissn = {1436-5073},
	orcid-numbers = {Kovács, Norbert/0000-0003-0753-0146; Gyurcsányi, Ervin Róbert/0000-0002-9929-7865}
}

@article{MTMT:34569163,
	title = {A generic approach based on long-lifetime fluorophores for the assessment of protein binding to polymer nanoparticles by fluorescence anisotropy},
	url = {https://m2.mtmt.hu/api/publication/34569163},
	author = {Ahmed, Marwa and Hessz, Dóra and Gyarmati, Benjámin Sándor and Pancsics, Mirko and Kovács, Norbert and Gyurcsányi, Ervin Róbert and Kubinyi, Miklós and Horváth, Viola},
	doi = {10.1039/d3nr02460a},
	journal-iso = {NANOSCALE},
	journal = {NANOSCALE},
	volume = {16},
	unique-id = {34569163},
	issn = {2040-3364},
	abstract = {Quantitation of protein-nanoparticle interactions is essential for the investigation of the protein corona around NPs in vivo and when using synthetic polymer nanoparticles as affinity reagents for selective protein recognition in vitro. Here, a method based on steady-state fluorescence anisotropy measurement is presented as a novel, separation-free tool for the assessment of protein-nanoparticle interactions. For this purpose, a long-lifetime luminescent Ru-complex is used for protein labelling, which exhibits low anisotropy when conjugated to the protein but displays high anisotropy when the proteins are bound to the much larger polymer nanoparticles. As a proof of concept, the interaction of lysozyme with poly(N-isopropylacrylamide-co-N-tert-butylacrylamide-co-acrylic acid) nanoparticles is studied, and fluorescence anisotropy measurements are used to establish the binding kinetics, binding isotherm and a competitive binding assay. Using long-lifetime fluorophores as protein labels, protein-nanoparticle interactions can be monitored through anisotropy change. Besides gaining thermodynamic and kinetic information on the binding process, competitive protein assays can be set up.},
	keywords = {SILICA; AFFINITY; POLARIZATION; ADSORPTION; GOLD NANOPARTICLES; lysozyme; Materials Science, Multidisciplinary; Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; EVALUATE; CORONA FORMATION},
	year = {2024},
	eissn = {2040-3372},
	pages = {3659-3667},
	orcid-numbers = {Hessz, Dóra/0000-0003-3033-2762; Kovács, Norbert/0000-0003-0753-0146; Gyurcsányi, Ervin Róbert/0000-0002-9929-7865; Horváth, Viola/0000-0003-1014-7930}
}

@article{MTMT:34192911,
	title = {Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein (vol 13, pg 1263, 2022)},
	url = {https://m2.mtmt.hu/api/publication/34192911},
	author = {Bognár, Zsófia and Supala, Eszter and Yarman, Aysu and Zhang, Xiaorong and Bier, Frank F. and Scheller, Frieder W. and Gyurcsányi, Ervin Róbert},
	doi = {10.1039/d3sc90170j},
	journal-iso = {CHEM SCI},
	journal = {CHEMICAL SCIENCE},
	volume = {14},
	unique-id = {34192911},
	issn = {2041-6520},
	abstract = {Correction for 'Peptide epitope-imprinted polymer microarrays for selective protein recognition. Application for SARS-CoV-2 RBD protein' by Zsofia Bognar et al., Chem. Sci., 2022, 13, 1263-1269, https://doi.org/10.1039/D1SC04502D.},
	year = {2023},
	eissn = {2041-6539},
	pages = {9980-9980},
	orcid-numbers = {Gyurcsányi, Ervin Róbert/0000-0002-9929-7865}
}

@article{MTMT:34085469,
	title = {Understanding the potentiometric response of cation-permselective hydrophilic nanopore-based electrodes in the low ion concentration range},
	url = {https://m2.mtmt.hu/api/publication/34085469},
	author = {Solymosi, Gergely Tamás and Gyurcsányi, Ervin Róbert},
	doi = {10.1016/j.elecom.2023.107540},
	journal-iso = {ELECTROCHEM COMMUN},
	journal = {ELECTROCHEMISTRY COMMUNICATIONS},
	volume = {153},
	unique-id = {34085469},
	issn = {1388-2481},
	year = {2023},
	eissn = {1873-1902},
	orcid-numbers = {Solymosi, Gergely Tamás/0000-0003-4006-0326; Gyurcsányi, Ervin Róbert/0000-0002-9929-7865}
}

@article{MTMT:34069356,
	title = {A kémiai érzékelés határainak feszegetése},
	url = {https://m2.mtmt.hu/api/publication/34069356},
	author = {Szalay, Péter},
	journal-iso = {MAGY KEM LAP},
	journal = {MAGYAR KÉMIKUSOK LAPJA},
	volume = {78},
	unique-id = {34069356},
	issn = {0025-0163},
	year = {2023},
	eissn = {1588-1199},
	pages = {108-110},
	orcid-numbers = {Szalay, Péter/0000-0003-1885-3557; Gyurcsányi, Ervin Róbert/0000-0002-9929-7865}
}

@article{MTMT:33727389,
	title = {Novel functional monomer for the electrochemical synthesis of highly affine epitope-imprinted polymers},
	url = {https://m2.mtmt.hu/api/publication/33727389},
	author = {Bognár, Zsófia and Kozma, József and Kovács, Norbert and Gyurcsányi, Ervin Róbert},
	doi = {10.1002/elan.202300025},
	journal-iso = {ELECTROANAL},
	journal = {ELECTROANALYSIS},
	volume = {35},
	unique-id = {33727389},
	issn = {1040-0397},
	year = {2023},
	eissn = {1521-4109},
	orcid-numbers = {Kovács, Norbert/0000-0003-0753-0146; Gyurcsányi, Ervin Róbert/0000-0002-9929-7865}
}

@article{MTMT:33453038,
	title = {Highly hydrophobic TEMPO-functionalized conducting copolymers for solid-contact ion-selective electrodes},
	url = {https://m2.mtmt.hu/api/publication/33453038},
	author = {Kozma, József and Papp, Soma and Gyurcsányi, Ervin Róbert},
	doi = {10.1016/j.bioelechem.2022.108352},
	journal-iso = {BIOELECTROCHEMISTRY},
	journal = {BIOELECTROCHEMISTRY},
	volume = {150},
	unique-id = {33453038},
	issn = {1567-5394},
	abstract = {Solid-contact ion-selective electrodes (SCISEs) emerged as the best electrode embodiment for miniaturized, wearable and disposable sensors for ion/electrolyte measurements in body fluids. The commercialization of inexpensive single-use "calibration-free" electrodes requires large scale manufacturing of electrodes with reproducible calibration parameters, e.g. E0. This is perhaps the most important shortcoming of SCISEs, beside the many advantages over their conventional liquid-contact counterparts. However, adjusting the E0 value for optimal potential stability is challenging for all state-of-the-art solid-contact materials, which may combine several types of transducing mechanism (e.g. capacitive and redox materials or their combination) for enhanced potential stability and analytical performance. Therefore, here we introduce for the first time the galvanostatic intermittent titration technique (GITT) to determine the best preadjusment potential. The proof of concept is shown for a novel type of solid-contact based on the copolymerization of 3,4-ethylenedioxythiophene with perfluorinated alkyl side chain (EDOTF) and (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl modified 3,4-ethylenedioxythiophene (EDOT-TEMPO). Such materials that are compliant with local electrodeposition and provide multiple functionalities, i.e. high hydrophobicity by the perfluorinated alkyl side chain, electron-to-ion transduction by the conducting polymer (EDOT) backbone and the confinement of well-defined redox couple (TEMPO), are expected to prevail as solid-contacts.},
	year = {2023},
	eissn = {1878-562X},
	orcid-numbers = {Papp, Soma/0000-0001-6625-8088; Gyurcsányi, Ervin Róbert/0000-0002-9929-7865}
}

@article{MTMT:33452970,
	title = {How an ACE2 mimicking epitope-MIP nanofilm recognizes template-related peptides and the receptor binding domain of SARS-CoV-2},
	url = {https://m2.mtmt.hu/api/publication/33452970},
	author = {Zhang, Xiaorong and Waffo, Armel T. and Yarman, Aysu and Kovács, Norbert and Bognár, Zsófia and Wollenberger, Ulla and El-Sherbiny, Ibrahim M. and Hassan, Rabeay Y. A. and Bier, Frank F. and Gyurcsányi, Ervin Róbert and Zebger, Ingo and Scheller, Frieder W.},
	doi = {10.1039/D2NR03898F},
	journal-iso = {NANOSCALE},
	journal = {NANOSCALE},
	volume = {14},
	unique-id = {33452970},
	issn = {2040-3364},
	abstract = {We developed original methods to confirm the liberation of the imprinted binding cavities by electrochemical template removal and identified the amino acid motif of the template which is determinant for the affinity of the epitope-imprinted polymer.},
	year = {2022},
	eissn = {2040-3372},
	pages = {18106-18114},
	orcid-numbers = {Zhang, Xiaorong/0000-0001-8079-1321; Waffo, Armel T./0000-0003-4021-1438; Kovács, Norbert/0000-0003-0753-0146; Bognár, Zsófia/0000-0001-9270-8863; El-Sherbiny, Ibrahim M./0000-0002-8179-437X; Gyurcsányi, Ervin Róbert/0000-0002-9929-7865; Zebger, Ingo/0000-0002-6354-3585}
}

@article{MTMT:32897099,
	title = {TEMPO-Functionalized Carbon Nanotubes for Solid-Contact Ion-Selective Electrodes with Largely Improved Potential Reproducibility and Stability},
	url = {https://m2.mtmt.hu/api/publication/32897099},
	author = {Kozma, József and Papp, Soma and Gyurcsányi, Ervin Róbert},
	doi = {10.1021/acs.analchem.2c00395},
	journal-iso = {ANAL CHEM},
	journal = {ANALYTICAL CHEMISTRY},
	volume = {94},
	unique-id = {32897099},
	issn = {0003-2700},
	year = {2022},
	eissn = {1520-6882},
	pages = {8249-8257},
	orcid-numbers = {Papp, Soma/0000-0001-6625-8088; Gyurcsányi, Ervin Róbert/0000-0002-9929-7865}
}

@article{MTMT:32761581,
	title = {In situ silver nanoparticle coating of virions for quantification at single virus level},
	url = {https://m2.mtmt.hu/api/publication/32761581},
	author = {Bognár, Zsófia and de, Jonge Marien I and Gyurcsányi, Ervin Róbert},
	doi = {10.1039/d1nr07607h},
	journal-iso = {NANOSCALE},
	journal = {NANOSCALE},
	volume = {14},
	unique-id = {32761581},
	issn = {2040-3364},
	abstract = {In situ labelling and encapsulation of biological entities, such as of single viruses, may provide a versatile approach to modulate their functionality and facilitate their detection at single particle level. Here, we introduce a novel virus metallization approach based on in situ coating of viruses in solution with silver nanoparticles (AgNP) in a two-step synthetic process, i.e. surface activation with a tannic acid - Sn(ii) coordination complex, which subsequently induces silver ion (i) reduction. The metalic coating on the virus surface opens the opportunity for electrochemical quantification of the AgNP-tagged viruses by nano-impact electrochemistry on a microelectrode with single particle sensitivity, i.e. enable the detection of particles oherwise undetectable. We show that the silver coating of the virus particles impacting the electrode can be oxidized to produce distinct current peaks the frequency of which show a linear correlation with the virus count. The proof of the concept was done with inactivated Influenza A (H3N2) viruses resulting in their quantitation down to the femtomolar concentrations (ca. 5 x 10(7) particles per mL) using 50 s counting sequences.},
	keywords = {ELECTROCHEMICAL DETECTION; LIVING CELLS; BACTERIA; particle; COLLISIONS; encapsulation; Graphene; YEAST-CELLS; Ultramicroelectrode; Materials Science, Multidisciplinary; Chemistry, Multidisciplinary; Nanoscience & Nanotechnology},
	year = {2022},
	eissn = {2040-3372},
	pages = {2296-2303},
	orcid-numbers = {Gyurcsányi, Ervin Róbert/0000-0002-9929-7865}
}