TY - JOUR AU - Gebri, Eniko AU - Hogyor, Kinga AU - Szabo, Adrienne AU - Guttman, András TI - Analysis of Soft Tissue N-Glycome Profiles in Oral Squamous Cell Carcinoma, a Pilot Study JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 27 PY - 2026 IS - 2 SP - 740 SN - 1661-6596 DO - 10.3390/ijms27020740 UR - https://m2.mtmt.hu/api/publication/36868436 ID - 36868436 AB - Oral squamous cell carcinoma (OSCC) is an aggressive disease with a glycoproteomically unmapped progression and a low five-year survival rate. Thus, the aim of this pilot study was to explore the N-glycosylation pattern differences in malignant, adjacent mucosal and healthy tissues in the context of OSCC. Oral mucosal soft tissue samples was obtained by incisional biopsy from five patients with OSCC, both from the malignant and the opposite healthy gingival sides, and from seven age-sex-matched healthy controls. The collected tissues were homogenized, followed by N-glycan profiling of the endoglycosidase-released and fluorophore-labeled carbohydrates using capillary electrophoresis with ultra-sensitive laser-induced fluorescent detection (CE-LIF). Six out of the twenty-two identified N-glycan structures, including glycogens, showed significant (p < 0.05) differences between the malignant tissue samples of the OSCC patients and the healthy controls. Comparing the healthy and the positive control oral mucosal samples, differences in four N-glycan structures were revealed, while only one alteration was observed between the N-glycan profiles of the malignant tumor and positive control samples. However, the results are presented descriptively, reflecting the limited sample size of the pilot study, it shows the potential of high-resolution CE-LIF-based glyocoanalytical protocol to be highly efficient and sensitive for glycobiomarker-based molecular diagnostics of oral malignant lesions. LA - English DB - MTMT ER - TY - JOUR AU - Ungvári, Anna Sára AU - Gulej, Rafal AU - Patai, Roland AU - Papp, Zoltán AU - Tóth, Attila AU - Szabó, Attila Ádám AU - Podesser, Bruno K. AU - Sótonyi, Péter AU - Benyó, Zoltán AU - Yabluchanskiy, Andriy AU - Tarantini, Stefano AU - Maier, Andrea B. AU - Csiszar, Anna AU - Ungvári, Zoltán István TI - Sex-specific mechanisms in vascular aging: exploring cellular and molecular pathways in the pathogenesis of age-related cardiovascular and cerebrovascular diseases JF - GEROSCIENCE: OFFICIAL JOURNAL OF THE AMERICAN AGING ASSOCIATION (AGE) J2 - GEROSCIENCE VL - 47 PY - 2025 IS - 1 SP - 301 EP - 337 PG - 37 SN - 2509-2715 DO - 10.1007/s11357-024-01489-2 UR - https://m2.mtmt.hu/api/publication/35663024 ID - 35663024 N1 - Anna Ungvari, Rafal Gulej, and Roland Patai contributed equally to this manuscript. AB - Aging remains the foremost risk factor for cardiovascular and cerebrovascular diseases, surpassing traditional factors in epidemiological significance. This review elucidates the cellular and molecular mechanisms underlying vascular aging, with an emphasis on sex differences that influence disease progression and clinical outcomes in older adults. We discuss the convergence of aging processes at the macro- and microvascular levels and their contributions to the pathogenesis of vascular diseases. Critical analysis of both preclinical and clinical studies reveals significant sex-specific variations in these mechanisms, which could be pivotal in understanding the disparity in disease morbidity and mortality between sexes. The review highlights key molecular pathways, including oxidative stress, inflammation, and autophagy, and their differential roles in the vascular aging of males and females. We argue that recognizing these sex-specific differences is crucial for developing targeted therapeutic strategies aimed at preventing and managing age-related vascular pathologies. The implications for personalized medicine and potential areas for future research are also explored, emphasizing the need for a nuanced approach to the study and treatment of vascular aging. LA - English DB - MTMT ER - TY - JOUR AU - Fekete, Mónika AU - Lehoczki, Andrea Marianna AU - Szappanos, Ágnes AU - Tóth, Attila AU - Mahdi, Mohamed AU - Sótonyi, Péter AU - Benyó, Zoltán AU - Yabluchanskiy, Andriy AU - Tarantini, Stefano AU - Ungvári, Zoltán István TI - Cerebromicrovascular mechanisms contributing to long COVID: implications for neurocognitive health JF - GEROSCIENCE: OFFICIAL JOURNAL OF THE AMERICAN AGING ASSOCIATION (AGE) J2 - GEROSCIENCE VL - 47 PY - 2025 IS - 1 SP - 745 EP - 779 PG - 35 SN - 2509-2715 DO - 10.1007/s11357-024-01487-4 UR - https://m2.mtmt.hu/api/publication/35676767 ID - 35676767 N1 - Monika Fekete, Andrea Lehoczki and Ágnes Szappanos contributed equally. AB - Long COVID (also known as post-acute sequelae of SARS-CoV-2 infection [PASC] or post-COVID syndrome) is characterized by persistent symptoms that extend beyond the acute phase of SARS-CoV-2 infection, affecting approximately 10% to over 30% of those infected. It presents a significant clinical challenge, notably due to pronounced neurocognitive symptoms such as brain fog. The mechanisms underlying these effects are multifactorial, with mounting evidence pointing to a central role of cerebromicrovascular dysfunction. This review investigates key pathophysiological mechanisms contributing to cerebrovascular dysfunction in long COVID and their impacts on brain health. We discuss how endothelial tropism of SARS-CoV-2 and direct vascular infection trigger endothelial dysfunction, impaired neurovascular coupling, and blood-brain barrier disruption, resulting in compromised cerebral perfusion. Furthermore, the infection appears to induce mitochondrial dysfunction, enhancing oxidative stress and inflammation within cerebral endothelial cells. Autoantibody formation following infection also potentially exacerbates neurovascular injury, contributing to chronic vascular inflammation and ongoing blood-brain barrier compromise. These factors collectively contribute to the emergence of white matter hyperintensities, promote amyloid pathology, and may accelerate neurodegenerative processes, including Alzheimer's disease. This review also emphasizes the critical role of advanced imaging techniques in assessing cerebromicrovascular health and the need for targeted interventions to address these cerebrovascular complications. A deeper understanding of the cerebrovascular mechanisms of long COVID is essential to advance targeted treatments and mitigate its long-term neurocognitive consequences. LA - English DB - MTMT ER - TY - JOUR AU - Auer, Felícia AU - Guttman, András TI - Effect of the operational parameters on the electromigration of proteins in sodium dodecyl sulfate capillary gel electrophoresis in the presence of propidium iodide fluorescent dye JF - TALANTA J2 - TALANTA VL - 287 PY - 2025 SP - 127601 SN - 0039-9140 DO - 10.1016/j.talanta.2025.127601 UR - https://m2.mtmt.hu/api/publication/35701407 ID - 35701407 N1 - Export Date: 30 January 2025; Cited By: 0; Correspondence Address: A. Guttman; Translational Glycomics Research Group, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Hungary; email: guttmanandras@med.unideb.hu; CODEN: TLNTA LA - English DB - MTMT ER - TY - JOUR AU - Török, Rebeka AU - Mészáros, Brigitta AU - Gombás, Veronika AU - Fogarassyné Vathy, Ágnes AU - Szabó, Miklós AU - Csánky, Eszter AU - Járvás, Gábor AU - Guttman, András TI - Predicting the effectiveness of chemotherapy treatment in lung cancer utilizing artificial intelligence-supported serum N-glycome analysis JF - COMPUTERS IN BIOLOGY AND MEDICINE J2 - COMPUT BIOL MED VL - 186 PY - 2025 PG - 7 SN - 0010-4825 DO - 10.1016/j.compbiomed.2025.109681 UR - https://m2.mtmt.hu/api/publication/35721307 ID - 35721307 N1 - Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Hungary Horváth Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Department of Computer Science and Systems Technology, University of Pannonia, Veszprem, Hungary Department of Pulmonology, Borsod Academic County Hospital, Miskolc, Hungary Export Date: 07 February 2025; Cited By: 0; Correspondence Address: A. Guttman; Horváth Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Doctoral School of Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary; email: guttmanandras@med.unideb.hu; CODEN: CBMDA LA - English DB - MTMT ER - TY - JOUR AU - Szerényi, Dóra AU - Járvás, Gábor AU - Guttman, András TI - Multifaceted Approaches in Epithelial Cell Adhesion Molecule-Mediated Circulating Tumor Cell Isolation JF - MOLECULES J2 - MOLECULES VL - 30 PY - 2025 IS - 5 PG - 18 SN - 1431-5157 DO - 10.3390/molecules30050976 UR - https://m2.mtmt.hu/api/publication/35775490 ID - 35775490 AB - Circulating tumor cells (CTCs) are pivotal in cancer metastasis and serve as valuable biomarkers for diagnosis, prognosis, and treatment monitoring. Traditional CTC capture methods predominantly utilize the epithelial cell adhesion molecule (EpCAM) as a marker for isolation. However, the heterogeneity of these circulating cells and the epithelial-to-mesenchymal transition process (wherein epithelial cells acquire mesenchymal characteristics) limit the efficacy of EpCAM-based capture techniques. In this paper, we critically review the role of the EpCAM in CTC capture, explore the impact of epithelial-to-mesenchymal transition on EpCAM expression, and discuss alternative biomarkers and strategies to enhance CTC isolation. By evaluating the limitations of EpCAM-mediated capture and the challenges posed by epithelial-to-mesenchymal transition, we aim to provide insights into the development of more comprehensive liquid biopsy approaches for cancer management. LA - English DB - MTMT ER - TY - JOUR AU - Farkas, Anna AU - Surányi, Andrea AU - Kolcsár, Bálint AU - Gyurkovits, Zita AU - Kozinszky, Zoltán AU - Sandor, G. Vari AU - Guttman, András TI - Potential Glycobiomarkers in Maternal Obesity and Gestational Diabetes During Human Pregnancy JF - JOURNAL OF CLINICAL MEDICINE J2 - J CLIN MED VL - 14 PY - 2025 IS - 5 PG - 15 SN - 2077-0383 DO - 10.3390/jcm14051626 UR - https://m2.mtmt.hu/api/publication/35795725 ID - 35795725 N1 - Nomination for the "International Research Awards on Sensing Technology" under the category of "Best Researcher Award". Entry ID: 6004 AB - Introduction: Obesity is a rapidly growing common health problem worldwide that can lead to the development of gestational diabetes mellitus (GDM). However, GDM not only affects women with obesity but can also develop at any time, even after the OGTT test; therefore, an increasing number of complications related to GDM can be seen in both mothers and their children. It is necessary to discover biomarkers capable of indicating the development of GDM or complications during/after pregnancy. Since the N-glycosylation motif of human IgG has been described to change under many physiological and pathological conditions, it is a promising target for biomarker research. In our study, the effects of obesity and GDM were investigated on human serum IgG N-linked glycosylation patterns during human pregnancy. Materials and Methods: The study participants were categorized into four groups according to their body mass index (BMI) and GDM status: normal weight as control, obese (BMI > 30 kg/m2), normal weight with GDM, and obese with GDM. The released N-glycan components of IgG were separated with capillary electrophoresis and detected using a laser-induced fluorescence detector. Results: The result revealed several differences between the N-glycosylation patterns of the four study groups. Of this, 17 of the 20 identified structures differed significantly between the groups. The ratios of sialylated to non-sialylated structures were not changed significantly, but the core fucosylation level showed a significant decrease in the GDM and obese GDM groups compared to the control subjects. The lowest degree of core fucosylation was observed in the GDM group. Conclusions: The findings indicate that obesity in isolation does not have a significant impact on the IgG N-glycosylation pattern in pregnancy. Conversely, alterations in the N-glycan profile of antibodies may serve as biomarkers for the diagnosis of GDM in mothers with a normal BMI, although more evidence is needed. By incorporating glycan-based biomarkers into clinical practice, healthcare providers can improve early detection, personalize management strategies, and potentially mitigate adverse pregnancy outcomes associated with obesity and GDM. Keywords: capillary electrophoresis; gestational diabetes mellitus; human IgG N-glycome; maternal obesity; molecular diagnostics LA - English DB - MTMT ER - TY - JOUR AU - Sárközy, Dániel AU - Farkas, Anna AU - Guttman, András TI - Rapid baseline hump-free analysis of therapeutic proteins in a wide molecular weight range by SDS - capillary agarose gel electrophoresis JF - ANALYTICA CHIMICA ACTA J2 - ANAL CHIM ACTA VL - 2025 PY - 2025 SP - 344147 SN - 0003-2670 DO - 10.1016/j.aca.2025.344147 UR - https://m2.mtmt.hu/api/publication/36123222 ID - 36123222 N1 - Horváth Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, Hungary Translational Glycomics Group, Research Institute of Biomolecular and Chemical Engineering, University of Pannonia, Egyetem u 10, Veszprem, Hungary Export Date: 29 July 2025; Cited By: 0; Correspondence Address: A. Guttman; Horváth Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, Hungary; email: guttmanandras@med.unideb.hu; CODEN: ACACA LA - English DB - MTMT ER - TY - JOUR AU - Fogarassyné Vathy, Ágnes AU - Gombás, Veronika AU - Török, Rebeka AU - Járvás, Gábor AU - Guttman, András TI - Improved analytical workflow towards machine learning supported N-glycomics-based biomarker discovery JF - TALANTA J2 - TALANTA VL - 295 PY - 2025 SP - 128389 SN - 0039-9140 DO - 10.1016/j.talanta.2025.128389 UR - https://m2.mtmt.hu/api/publication/36172614 ID - 36172614 N1 - Cited by: 0 LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Andrea AU - Jeney, Viktória TI - Hypoxia-Induced Changes in Endothelial Cell Phenotype and Function JF - ANTIOXIDANTS & REDOX SIGNALING J2 - ANTIOXID REDOX SIGNAL PY - 2025 SN - 1523-0864 DO - 10.1089/ars.2025.1022 UR - https://m2.mtmt.hu/api/publication/36189639 ID - 36189639 N1 - Export Date: 12 June 2025; Cited By: 0; Correspondence Address: V. Jeney; MTA-DE Lendület Vascular Pathophysiology Research Group, Research Centre for Molecular Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Nagyerdei Krt. 98., 4032, Hungary; email: jeney.viktoria@med.unideb.hu; CODEN: ARSIF AB - Significance: Endothelial cells (ECs) are specialized cells lining the interior surface of blood vessels, playing a crucial role in vascular biology. They exhibit remarkable versatility, adapting to various tissue requirements. Their ability to respond to physiological and pathological stimuli ensures proper tissue function and homeostasis. Recent Advances: Hypoxia is when the oxygen level in a given organ, tissue, or cell type drops below the physiological level and is insufficient to maintain adequate homeostasis. ECs respond to hypoxia by activating various mechanisms. Hypoxia-induced changes in ECs can promote survival in low-oxygen environments by altering cellular metabolism and inducing neoangiogenesis. However, hypoxia-induced EC responses can also be detrimental, leading to increased production of reactive oxygen species, heightened inflammation, changes in vascular tone, increased permeability of the endothelial barrier, and a higher risk of coagulation. Critical Issues: Hypoxia-induced EC responses contribute to the pathogenesis of various diseases, including metabolic diseases (e.g., diabetes, chronic kidney disease), infectious diseases, chronic inflammation, neoplastic diseases, cardiovascular diseases (e.g., atherosclerosis, myocardial infarction, and stroke) lung diseases (e.g., chronic obstructive pulmonary disease and pulmonary hypertension), eye diseases (age-related macular degeneration and retinopathy), and neurodegenerative diseases (e.g., Alzheimer’s disease and Parkinson’s disease). Future Directions: Detailed, disease-specific investigations are essential to delineate how endothelial hypoxia responses contribute to various pathologies. Understanding these mechanisms could reveal whether targeting endothelial hypoxia holds therapeutic potential. Antioxid. Redox Signal. 00, 000-000. Copyright 2025, Mary Ann Liebert, Inc., publishers. LA - English DB - MTMT ER -