TY - JOUR AU - Al-Smadi, Mohammad Walid AU - Fazekas, Laszlo Adam AU - Varga, Adam AU - Matrai, Adam Attila AU - Aslan, Siran AU - Beqain, Anas AU - Al-Khafaji, Mustafa Qais Muhsin AU - Baráth, Barbara AU - Novák, László AU - Németh, Norbert TI - Minor micro-rheological alterations in the presence of an artificial saphenous arteriovenous shunt, as an arteriovenous malformation model in the rat JF - CLINICAL HEMORHEOLOGY AND MICROCIRCULATION J2 - CLIN HEMORHEOL MICRO PY - 2024 SP - 1 EP - 11 PG - 11 SN - 1386-0291 DO - 10.3233/CH-231825 UR - https://m2.mtmt.hu/api/publication/34558196 ID - 34558196 AB - BACKGROUND: Arteriovenous malformations (AVMs) are vascular anomalies characterized by abnormal shunting between arteries and veins. The progression of the AVMs and their hemodynamic and rheological relations are poorly studied, and there is a lack of a feasible experimental model. OBJECTIVE: To establish a model that cause only minimal micro-rheological alterations, compared to other AV models. METHODS: Sixteen female Sprague Dawley rats were randomly divided into control and AVM groups. End-to-end anastomoses were created between the saphenous veins and arteries to mimic AVM nidus. Hematological and hemorheological parameters were analyzed before surgery and on the 1st, 3rd, 5th, 7th, 9th, and 12th postoperative weeks. RESULTS: Compared to sham-operated Control group the AVM group did not show important alterations in hematological parameters nor in erythrocyte aggregation and deformability. However, slightly increased aggregation and moderately decreased deformability values were found, without significant differences. The changes normalized by the 12th postoperative week. CONCLUSIONS: The presented rat model of a small-caliber AVM created on saphenous vessels does not cause significant micro-rheological changes. The alterations found were most likely related to the acute phase reactions and not to the presence of a small-caliber shunt. The model seems to be suitable for further studies of AVM progression. LA - English DB - MTMT ER - TY - JOUR AU - Géczi, Dóra Anikó AU - Nagy, Bálint AU - Szilágyi-Bónizs, Melinda AU - Penyige, András AU - Klekner, Álmos AU - Virga, József AU - Birkó, Zsuzsanna TI - Analysis of circulating miRNA profile in plasma sapmles of glioblastoma patients JF - EUROPEAN JOURNAL OF HUMAN GENETICS J2 - EUR J HUM GENET VL - 31 PY - 2023 IS - S1 SP - 546 EP - 547 PG - 2 SN - 1018-4813 UR - https://m2.mtmt.hu/api/publication/34732037 ID - 34732037 LA - English DB - MTMT ER - TY - JOUR AU - Berki, A. AU - Palotai, M. AU - Muthuraman, M. AU - Ding, H. AU - Halasz, L. AU - Eross, L. AU - Fekete, G. AU - Bognár, László AU - Pichner, E. AU - Tamas, G. TI - Quantifying the effect of subthalamic stimulation on bradykinesia using digitized spiral drawings in Parkinson's disease JF - EUROPEAN JOURNAL OF NEUROLOGY J2 - EUR J NEUROL VL - 30 PY - 2023 IS - Supplement_1 SP - 231 EP - 231 PG - 1 SN - 1351-5101 UR - https://m2.mtmt.hu/api/publication/34718768 ID - 34718768 LA - English DB - MTMT ER - TY - JOUR AU - Csámer, Loránd AU - Csernátony, Zoltán AU - Novák, László AU - Kővári, Viktor Zsolt AU - Kovács, Ágnes Éva AU - Soósné Horváth, Hajnalka AU - Manó, Sándor TI - Custom-made 3D printing-based cranioplasty using a silicone mould and PMMA JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 15 SN - 2045-2322 DO - 10.1038/s41598-023-38772-9 UR - https://m2.mtmt.hu/api/publication/34075903 ID - 34075903 AB - All types of cranioplasty techniques restore the morphology of the skull and affect patient aesthetics. Safe and easy techniques are required to enhance patients’ recovery and the rehabilitation process. We propose a new method of cranioplasty. The 3-dimensional (3D) reconstruction of a thin-layer computed tomography (CT) scan of the skull was used to reflect the intact side onto the defect and subtract the overlapping points from one another. In this way, a 3D model of the planned implant can be built in the required shape and size. The precise fit of the implant can be checked by printing the defective part of the skull in case it can be modified. A sterilisable silicone mould based on the finalized model was created afterwards. Polymethyl methacrylate implants were prepared directly in an aseptic environment in the operating room during surgery. Between 2005 and 2020, we performed 54 cranioplasties on 52 patients whose craniotomies were performed previously for indications of traumatic brain injury, stroke or tumour surgeries. No technical problems were noted during the operations. In 2 cases, septic complications that occurred were not connected to the technique itself, and the implants were removed and later replaced. Our proposed technique based on 3D-printed individual silicone moulds is a reliable, safe, easily reproducible and low-cost method to repair different skull defects. LA - English DB - MTMT ER - TY - JOUR AU - Loevner, Laurie A AU - Kolumban, Balint AU - Hutóczki, Gábor AU - Dziadziuszko, Katarzyna AU - Bereczki, Dániel AU - Bagó, Attila György AU - Pichiecchio, Anna TI - Efficacy and Safety of Gadopiclenol for Contrast-Enhanced MRI of the Central Nervous System. The PICTURE Randomized Clinical Trial. TS - The PICTURE Randomized Clinical Trial. JF - INVESTIGATIVE RADIOLOGY J2 - INVEST RADIOL VL - 58 PY - 2023 IS - 5 SP - 307 EP - 313 PG - 7 SN - 0020-9996 DO - 10.1097/RLI.0000000000000944 UR - https://m2.mtmt.hu/api/publication/33623134 ID - 33623134 N1 - Department of Radiology, University of Pennsylvania, Philadelphia, PA, United States Department of Neurosurgery, University of Pécs, Pécs, Hungary Department of Neurosurgery, University of Debrecen, Debrecen, Hungary Department of Radiology Early Clinical Trials Centre, Medical University of Gdansk, Gdansk, Poland Department of Neurology, Semmelweis University, Hungary Department of Neuro-oncology, National Institute of Clinical Neurosciences, Budapest, Hungary Department of Brain and Behavioral Sciences, University of Pavia, Italy Department of Neuroradiology, IRCCS, Mondino Foundation, Pavia, Italy Cited By :4 Export Date: 3 October 2023 CODEN: INVRA Correspondence Address: Loevner, L.A.; Department of Radiology, 3400 Spruce St., United States; email: Laurie.Loevner@pennmedicine.upenn.edu AB - Developing new high relaxivity gadolinium-based contrast agents (GBCAs) for magnetic resonance imaging (MRI) allowing dose reduction while maintaining similar diagnostic efficacy is needed, especially in the context of gadolinium retention in tissues. This study aimed to demonstrate that contrast-enhanced MRI of the central nervous system (CNS) with gadopiclenol at 0.05 mmol/kg is not inferior to gadobutrol at 0.1 mmol/kg, and superior to unenhanced MRI.PICTURE is an international, randomized, double-blinded, controlled, cross-over, phase III study, conducted between June 2019 and September 2020. Adult patients with CNS lesions were randomized to undergo 2 MRIs (interval, 2-14 days) with gadopiclenol (0.05 mmol/kg) then gadobutrol (0.1 mmol/kg) or vice versa. The primary criterion was lesion visualization based on 3 parameters (border delineation, internal morphology, and contrast enhancement), assessed by 3 off-site blinded readers. Key secondary outcomes included lesion-to-background ratio, enhancement percentage, contrast-to-noise ratio, overall diagnostic preference, and adverse events.Of the 256 randomized patients, 250 received at least 1 GBCA administration (mean [SD] age, 57.2 [13.8] years; 53.6% women). The statistical noninferiority of gadopiclenol (0.05 mmol/kg) to gadobutrol (0.1 mmol/kg) was achieved for all parameters and all readers (n = 236, lower limit 95% confidence interval of the difference ≥-0.06, above the noninferiority margin [-0.35], P < 0.0001), as well as its statistical superiority over unenhanced images (n = 239, lower limit 95% confidence interval of the difference ≥1.29, P < 0.0001).Enhancement percentage and lesion-to-background ratio were higher with gadopiclenol for all readers (P < 0.0001), and contrast-to-noise ratio was higher for 2 readers (P = 0.02 and P < 0.0001). Three blinded readers preferred images with gadopiclenol for 44.8%, 54.4%, and 57.3% of evaluations, reported no preference for 40.7%, 21.6%, and 23.2%, and preferred images with gadobutrol for 14.5%, 24.1%, and 19.5% (P < 0.001).Adverse events reported after MRI were similar for gadopiclenol (14.6% of patients) and gadobutrol (17.6%). Adverse events considered related to gadopiclenol (4.9%) and gadobutrol (6.9%) were mainly injection site reactions, and none was serious.Gadopiclenol at 0.05 mmol/kg is not inferior to gadobutrol at 0.1 mmol/kg for MRI of the CNS, confirming that gadopiclenol can be used at half the gadolinium dose used for other GBCAs to achieve similar clinical efficacy. LA - English DB - MTMT ER - TY - JOUR AU - Dobra, Gabriella AU - Gyukity-Sebestyén, Edina AU - Bukva, Mátyás AU - Harmati, Mária AU - Nagy, Valentina AU - Szabó, Zoltán AU - Pankotai, Tibor AU - Klekner, Álmos AU - Buzás, Krisztina TI - MMP-9 as Prognostic Marker for Brain Tumours: A Comparative Study on Serum-Derived Small Extracellular Vesicles JF - CANCERS J2 - CANCERS VL - 15 PY - 2023 IS - 3 PG - 21 SN - 2072-6694 DO - 10.3390/cancers15030712 UR - https://m2.mtmt.hu/api/publication/33592853 ID - 33592853 N1 - Laboratory of Microscopic Image Analysis and Machine Learning, Institute of Biochemistry, Biological Research Centre, Eötvös Loránd Research Network (ELKH), Szeged, H-6726, Hungary Doctoral School of Interdisciplinary Medicine, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, H-6720, Hungary Department of Immunology, Albert Szent-Györgyi Medical School, Faculty of Science and Informatics, University of Szeged, Szeged, H-6720, Hungary Department of Medical Chemistry, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, H-6720, Hungary Institute of Pathology, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, H-6720, Hungary Genome Integrity and DNA Repair Group, Hungarian Centre of Excellence for Molecular Medicine (HCEMM), University of Szeged, Szeged, H-6720, Hungary Department of Neurosurgery, Faculty of Medicine, University of Debrecen, Debrecen, H-4032, Hungary Export Date: 8 March 2023 Correspondence Address: Buzás, K.; Laboratory of Microscopic Image Analysis and Machine Learning, Hungary; email: buzas.krisztina@brc.hu AB - Matrix metalloproteinase-9 (MMP-9) degrades the extracellular matrix, contributes to tumour cell invasion and metastasis, and its elevated level in brain tumour tissues indicates poor prognosis. High-risk tissue biopsy can be replaced by liquid biopsy; however, the blood–brain barrier (BBB) prevents tumour-associated components from entering the peripheral blood, making the development of blood-based biomarkers challenging. Therefore, we examined the MMP-9 content of small extracellular vesicles (sEVs)—which can cross the BBB and are stable in body fluids—to characterise tumours with different invasion capacity. From four patient groups (glioblastoma multiforme, brain metastases of lung cancer, meningioma, and lumbar disc herniation as controls), 222 serum-derived sEV samples were evaluated. After isolating and characterising sEVs, their MMP-9 content was measured by ELISA and assessed statistically (correlation, paired t-test, Welch’s test, ANOVA, ROC). We found that the MMP-9 content of sEVs is independent of gender and age, but is affected by surgical intervention, treatment, and recurrence. We found a relation between low MMP-9 level in sEVs (<28 ppm) and improved survival (8-month advantage) of glioblastoma patients, and MMP-9 levels showed a positive correlation with aggressiveness. These findings suggest that vesicular MMP-9 level might be a useful prognostic marker for brain tumours. LA - English DB - MTMT ER - TY - BOOK ED - Novák, László ED - Volodymyr, Smolanka TI - Implementation of new standards and technologies for surgical treatment of central nervous system diseases in cross-border region (HUSKROUA 1901/8.2/0074) PB - Debreceni Egyetem CY - Debrecen PY - 2022 UR - https://m2.mtmt.hu/api/publication/33688912 ID - 33688912 LA - English DB - MTMT ER - TY - JOUR AU - Virga, József TI - Primer központi idegrendszeri daganatok ellátása : Összefoglaló klinikai onkológusok számára JF - KLINIKAI ONKOLÓGIA J2 - KLINIKAI ONKOLÓGIA VL - 9 PY - 2022 IS - 4 SP - 369 EP - 376 PG - 8 SN - 2064-5058 UR - https://m2.mtmt.hu/api/publication/33688802 ID - 33688802 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Fehér, Ádám AU - Pethő, Zoltán Dénes AU - Szántó, Gábor Tibor AU - Klekner, Álmos AU - Tajti, Gábor AU - Batta, Gyula Gábor (Ifj.) AU - Hortobágyi, Tibor AU - Varga, Zoltán AU - Schwab, Albrecht AU - Panyi, György TI - Mapping the functional expression of auxiliary subunits of KCa1.1 in glioblastoma JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 12 PY - 2022 IS - 1 PG - 14 SN - 2045-2322 DO - 10.1038/s41598-022-26196-w UR - https://m2.mtmt.hu/api/publication/33398474 ID - 33398474 AB - Glioblastoma (GBM) is the most aggressive glial tumor, where ion channels, including K Ca 1.1, are candidates for new therapeutic options. Since the auxiliary subunits linked to K Ca 1.1 in GBM are largely unknown we used electrophysiology combined with pharmacology and gene silencing to address the functional expression of K Ca 1.1/ β subunits complexes in both primary tumor cells and in the glioblastoma cell line U-87 MG. The pattern of the sensitivity (activation/inhibition) of the whole-cell currents to paxilline, lithocholic acid, arachidonic acid, and iberiotoxin; the presence of inactivation of the whole-cell current along with the loss of the outward rectification upon exposure to the reducing agent DTT collectively argue that K Ca 1.1/β3 complex is expressed in U-87 MG. Similar results were found using human primary glioblastoma cells isolated from patient samples. Silencing the β3 subunit expression inhibited carbachol-induced Ca 2+ transients in U-87 MG thereby indicating the role of the K Ca 1.1/β3 in the Ca 2+ signaling of glioblastoma cells. Functional expression of the K Ca 1.1/β3 complex, on the other hand, lacks cell cycle dependence. We suggest that the K Ca 1.1/β3 complex may have diagnostic and therapeutic potential in glioblastoma in the future. LA - English DB - MTMT ER - TY - JOUR AU - Soltész, Beáta AU - Pös, Ondrej AU - Wlachovska, Zuzana AU - Budis, Jaroslav AU - Hekel, Rastislav AU - Strieskova, Lucia AU - Liptak, Jana Bozenka AU - Krampl, Werner AU - Styk, Jakub AU - Németh , Nikolett AU - Keserű, Judit Szilvia AU - Jenei, Adrienn AU - Buglyó, Gergely AU - Klekner, Álmos AU - Nagy, Bálint AU - Szemes, Tomas TI - Mitochondrial DNA copy number changes, heteroplasmy, and mutations in plasma-derived exosomes and brain tissue of glioblastoma patients JF - MOLECULAR AND CELLULAR PROBES J2 - MOL CELL PROBE VL - 66 PY - 2022 PG - 6 SN - 0890-8508 DO - 10.1016/j.mcp.2022.101875 UR - https://m2.mtmt.hu/api/publication/33291817 ID - 33291817 LA - English DB - MTMT ER -