@article{MTMT:34558196, title = {Minor micro-rheological alterations in the presence of an artificial saphenous arteriovenous shunt, as an arteriovenous malformation model in the rat}, url = {https://m2.mtmt.hu/api/publication/34558196}, author = {Al-Smadi, Mohammad Walid and Fazekas, Laszlo Adam and Varga, Adam and Matrai, Adam Attila and Aslan, Siran and Beqain, Anas and Al-Khafaji, Mustafa Qais Muhsin and Baráth, Barbara and Novák, László and Németh, Norbert}, doi = {10.3233/CH-231825}, journal-iso = {CLIN HEMORHEOL MICRO}, journal = {CLINICAL HEMORHEOLOGY AND MICROCIRCULATION}, unique-id = {34558196}, issn = {1386-0291}, abstract = {BACKGROUND: Arteriovenous malformations (AVMs) are vascular anomalies characterized by abnormal shunting between arteries and veins. The progression of the AVMs and their hemodynamic and rheological relations are poorly studied, and there is a lack of a feasible experimental model. OBJECTIVE: To establish a model that cause only minimal micro-rheological alterations, compared to other AV models. METHODS: Sixteen female Sprague Dawley rats were randomly divided into control and AVM groups. End-to-end anastomoses were created between the saphenous veins and arteries to mimic AVM nidus. Hematological and hemorheological parameters were analyzed before surgery and on the 1st, 3rd, 5th, 7th, 9th, and 12th postoperative weeks. RESULTS: Compared to sham-operated Control group the AVM group did not show important alterations in hematological parameters nor in erythrocyte aggregation and deformability. However, slightly increased aggregation and moderately decreased deformability values were found, without significant differences. The changes normalized by the 12th postoperative week. CONCLUSIONS: The presented rat model of a small-caliber AVM created on saphenous vessels does not cause significant micro-rheological changes. The alterations found were most likely related to the acute phase reactions and not to the presence of a small-caliber shunt. The model seems to be suitable for further studies of AVM progression.}, year = {2024}, eissn = {1875-8622}, pages = {1-11}, orcid-numbers = {Németh, Norbert/0000-0002-1162-3778} } @article{MTMT:34732037, title = {Analysis of circulating miRNA profile in plasma sapmles of glioblastoma patients}, url = {https://m2.mtmt.hu/api/publication/34732037}, author = {Géczi, Dóra Anikó and Nagy, Bálint and Szilágyi-Bónizs, Melinda and Penyige, András and Klekner, Álmos and Virga, József and Birkó, Zsuzsanna}, journal-iso = {EUR J HUM GENET}, journal = {EUROPEAN JOURNAL OF HUMAN GENETICS}, volume = {31}, unique-id = {34732037}, issn = {1018-4813}, year = {2023}, eissn = {1476-5438}, pages = {546-547}, orcid-numbers = {Nagy, Bálint/0000-0002-0295-185X} } @article{MTMT:34718768, title = {Quantifying the effect of subthalamic stimulation on bradykinesia using digitized spiral drawings in Parkinson's disease}, url = {https://m2.mtmt.hu/api/publication/34718768}, author = {Berki, A. and Palotai, M. and Muthuraman, M. and Ding, H. and Halasz, L. and Eross, L. and Fekete, G. and Bognár, László and Pichner, E. and Tamas, G.}, journal-iso = {EUR J NEUROL}, journal = {EUROPEAN JOURNAL OF NEUROLOGY}, volume = {30}, unique-id = {34718768}, issn = {1351-5101}, year = {2023}, eissn = {1468-1331}, pages = {231-231} } @article{MTMT:34075903, title = {Custom-made 3D printing-based cranioplasty using a silicone mould and PMMA}, url = {https://m2.mtmt.hu/api/publication/34075903}, author = {Csámer, Loránd and Csernátony, Zoltán and Novák, László and Kővári, Viktor Zsolt and Kovács, Ágnes Éva and Soósné Horváth, Hajnalka and Manó, Sándor}, doi = {10.1038/s41598-023-38772-9}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {13}, unique-id = {34075903}, issn = {2045-2322}, abstract = {All types of cranioplasty techniques restore the morphology of the skull and affect patient aesthetics. Safe and easy techniques are required to enhance patients’ recovery and the rehabilitation process. We propose a new method of cranioplasty. The 3-dimensional (3D) reconstruction of a thin-layer computed tomography (CT) scan of the skull was used to reflect the intact side onto the defect and subtract the overlapping points from one another. In this way, a 3D model of the planned implant can be built in the required shape and size. The precise fit of the implant can be checked by printing the defective part of the skull in case it can be modified. A sterilisable silicone mould based on the finalized model was created afterwards. Polymethyl methacrylate implants were prepared directly in an aseptic environment in the operating room during surgery. Between 2005 and 2020, we performed 54 cranioplasties on 52 patients whose craniotomies were performed previously for indications of traumatic brain injury, stroke or tumour surgeries. No technical problems were noted during the operations. In 2 cases, septic complications that occurred were not connected to the technique itself, and the implants were removed and later replaced. Our proposed technique based on 3D-printed individual silicone moulds is a reliable, safe, easily reproducible and low-cost method to repair different skull defects.}, year = {2023}, eissn = {2045-2322}, orcid-numbers = {Csámer, Loránd/0000-0002-5023-3617; Kővári, Viktor Zsolt/0000-0002-5747-1167} } @article{MTMT:33623134, title = {Efficacy and Safety of Gadopiclenol for Contrast-Enhanced MRI of the Central Nervous System. The PICTURE Randomized Clinical Trial.}, url = {https://m2.mtmt.hu/api/publication/33623134}, author = {Loevner, Laurie A and Kolumban, Balint and Hutóczki, Gábor and Dziadziuszko, Katarzyna and Bereczki, Dániel and Bagó, Attila György and Pichiecchio, Anna}, doi = {10.1097/RLI.0000000000000944}, journal-iso = {INVEST RADIOL}, journal = {INVESTIGATIVE RADIOLOGY}, volume = {58}, unique-id = {33623134}, issn = {0020-9996}, abstract = {Developing new high relaxivity gadolinium-based contrast agents (GBCAs) for magnetic resonance imaging (MRI) allowing dose reduction while maintaining similar diagnostic efficacy is needed, especially in the context of gadolinium retention in tissues. This study aimed to demonstrate that contrast-enhanced MRI of the central nervous system (CNS) with gadopiclenol at 0.05 mmol/kg is not inferior to gadobutrol at 0.1 mmol/kg, and superior to unenhanced MRI.PICTURE is an international, randomized, double-blinded, controlled, cross-over, phase III study, conducted between June 2019 and September 2020. Adult patients with CNS lesions were randomized to undergo 2 MRIs (interval, 2-14 days) with gadopiclenol (0.05 mmol/kg) then gadobutrol (0.1 mmol/kg) or vice versa. The primary criterion was lesion visualization based on 3 parameters (border delineation, internal morphology, and contrast enhancement), assessed by 3 off-site blinded readers. Key secondary outcomes included lesion-to-background ratio, enhancement percentage, contrast-to-noise ratio, overall diagnostic preference, and adverse events.Of the 256 randomized patients, 250 received at least 1 GBCA administration (mean [SD] age, 57.2 [13.8] years; 53.6% women). The statistical noninferiority of gadopiclenol (0.05 mmol/kg) to gadobutrol (0.1 mmol/kg) was achieved for all parameters and all readers (n = 236, lower limit 95% confidence interval of the difference ≥-0.06, above the noninferiority margin [-0.35], P < 0.0001), as well as its statistical superiority over unenhanced images (n = 239, lower limit 95% confidence interval of the difference ≥1.29, P < 0.0001).Enhancement percentage and lesion-to-background ratio were higher with gadopiclenol for all readers (P < 0.0001), and contrast-to-noise ratio was higher for 2 readers (P = 0.02 and P < 0.0001). Three blinded readers preferred images with gadopiclenol for 44.8%, 54.4%, and 57.3% of evaluations, reported no preference for 40.7%, 21.6%, and 23.2%, and preferred images with gadobutrol for 14.5%, 24.1%, and 19.5% (P < 0.001).Adverse events reported after MRI were similar for gadopiclenol (14.6% of patients) and gadobutrol (17.6%). Adverse events considered related to gadopiclenol (4.9%) and gadobutrol (6.9%) were mainly injection site reactions, and none was serious.Gadopiclenol at 0.05 mmol/kg is not inferior to gadobutrol at 0.1 mmol/kg for MRI of the CNS, confirming that gadopiclenol can be used at half the gadolinium dose used for other GBCAs to achieve similar clinical efficacy.}, year = {2023}, eissn = {1536-0210}, pages = {307-313}, orcid-numbers = {Bereczki, Dániel/0000-0002-8374-0500} } @article{MTMT:33592853, title = {MMP-9 as Prognostic Marker for Brain Tumours: A Comparative Study on Serum-Derived Small Extracellular Vesicles}, url = {https://m2.mtmt.hu/api/publication/33592853}, author = {Dobra, Gabriella and Gyukity-Sebestyén, Edina and Bukva, Mátyás and Harmati, Mária and Nagy, Valentina and Szabó, Zoltán and Pankotai, Tibor and Klekner, Álmos and Buzás, Krisztina}, doi = {10.3390/cancers15030712}, journal-iso = {CANCERS}, journal = {CANCERS}, volume = {15}, unique-id = {33592853}, abstract = {Matrix metalloproteinase-9 (MMP-9) degrades the extracellular matrix, contributes to tumour cell invasion and metastasis, and its elevated level in brain tumour tissues indicates poor prognosis. High-risk tissue biopsy can be replaced by liquid biopsy; however, the blood–brain barrier (BBB) prevents tumour-associated components from entering the peripheral blood, making the development of blood-based biomarkers challenging. Therefore, we examined the MMP-9 content of small extracellular vesicles (sEVs)—which can cross the BBB and are stable in body fluids—to characterise tumours with different invasion capacity. From four patient groups (glioblastoma multiforme, brain metastases of lung cancer, meningioma, and lumbar disc herniation as controls), 222 serum-derived sEV samples were evaluated. After isolating and characterising sEVs, their MMP-9 content was measured by ELISA and assessed statistically (correlation, paired t-test, Welch’s test, ANOVA, ROC). We found that the MMP-9 content of sEVs is independent of gender and age, but is affected by surgical intervention, treatment, and recurrence. We found a relation between low MMP-9 level in sEVs (<28 ppm) and improved survival (8-month advantage) of glioblastoma patients, and MMP-9 levels showed a positive correlation with aggressiveness. These findings suggest that vesicular MMP-9 level might be a useful prognostic marker for brain tumours.}, year = {2023}, eissn = {2072-6694}, orcid-numbers = {Dobra, Gabriella/0000-0002-2814-7720; Gyukity-Sebestyén, Edina/0000-0003-1383-6301; Bukva, Mátyás/0000-0002-5225-0285; Harmati, Mária/0000-0002-4875-5723; Szabó, Zoltán/0000-0001-8278-8038; Pankotai, Tibor/0000-0001-9810-5465; Buzás, Krisztina/0000-0001-8933-2033} } @book{MTMT:33688912, title = {Implementation of new standards and technologies for surgical treatment of central nervous system diseases in cross-border region (HUSKROUA 1901/8.2/0074)}, url = {https://m2.mtmt.hu/api/publication/33688912}, editor = {Novák, László and Volodymyr, Smolanka}, publisher = {University of Debrecen}, unique-id = {33688912}, year = {2022} } @article{MTMT:33688802, title = {Primer központi idegrendszeri daganatok ellátása : Összefoglaló klinikai onkológusok számára}, url = {https://m2.mtmt.hu/api/publication/33688802}, author = {Virga, József}, journal-iso = {KLINIKAI ONKOLÓGIA}, journal = {KLINIKAI ONKOLÓGIA}, volume = {9}, unique-id = {33688802}, issn = {2064-5058}, year = {2022}, pages = {369-376} } @article{MTMT:33398474, title = {Mapping the functional expression of auxiliary subunits of KCa1.1 in glioblastoma}, url = {https://m2.mtmt.hu/api/publication/33398474}, author = {Fehér, Ádám and Pethő, Zoltán Dénes and Szántó, Gábor Tibor and Klekner, Álmos and Tajti, Gábor and Batta, Gyula Gábor (Ifj.) and Hortobágyi, Tibor and Varga, Zoltán and Schwab, Albrecht and Panyi, György}, doi = {10.1038/s41598-022-26196-w}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {12}, unique-id = {33398474}, issn = {2045-2322}, abstract = {Glioblastoma (GBM) is the most aggressive glial tumor, where ion channels, including K Ca 1.1, are candidates for new therapeutic options. Since the auxiliary subunits linked to K Ca 1.1 in GBM are largely unknown we used electrophysiology combined with pharmacology and gene silencing to address the functional expression of K Ca 1.1/ β subunits complexes in both primary tumor cells and in the glioblastoma cell line U-87 MG. The pattern of the sensitivity (activation/inhibition) of the whole-cell currents to paxilline, lithocholic acid, arachidonic acid, and iberiotoxin; the presence of inactivation of the whole-cell current along with the loss of the outward rectification upon exposure to the reducing agent DTT collectively argue that K Ca 1.1/β3 complex is expressed in U-87 MG. Similar results were found using human primary glioblastoma cells isolated from patient samples. Silencing the β3 subunit expression inhibited carbachol-induced Ca 2+ transients in U-87 MG thereby indicating the role of the K Ca 1.1/β3 in the Ca 2+ signaling of glioblastoma cells. Functional expression of the K Ca 1.1/β3 complex, on the other hand, lacks cell cycle dependence. We suggest that the K Ca 1.1/β3 complex may have diagnostic and therapeutic potential in glioblastoma in the future.}, year = {2022}, eissn = {2045-2322}, orcid-numbers = {Batta, Gyula Gábor (Ifj.)/0000-0001-8735-6920; Hortobágyi, Tibor/0000-0001-5732-7942; Panyi, György/0000-0001-6227-3301} } @article{MTMT:33291817, title = {Mitochondrial DNA copy number changes, heteroplasmy, and mutations in plasma-derived exosomes and brain tissue of glioblastoma patients}, url = {https://m2.mtmt.hu/api/publication/33291817}, author = {Soltész, Beáta and Pös, Ondrej and Wlachovska, Zuzana and Budis, Jaroslav and Hekel, Rastislav and Strieskova, Lucia and Liptak, Jana Bozenka and Krampl, Werner and Styk, Jakub and Németh , Nikolett and Keserű, Judit Szilvia and Jenei, Adrienn and Buglyó, Gergely and Klekner, Álmos and Nagy, Bálint and Szemes, Tomas}, doi = {10.1016/j.mcp.2022.101875}, journal-iso = {MOL CELL PROBE}, journal = {MOLECULAR AND CELLULAR PROBES}, volume = {66}, unique-id = {33291817}, issn = {0890-8508}, year = {2022}, eissn = {1096-1194}, orcid-numbers = {Buglyó, Gergely/0000-0001-5994-2658; Nagy, Bálint/0000-0002-0295-185X} }