TY - JOUR AU - Homolya, L AU - Mathomes, RT AU - Fodor-Varga, Luca Anna AU - Docsa, Tibor AU - Juhász, L AU - Hayes, JM AU - Somsák, L TI - Synthesis, in silico and kinetics evaluation of N-(beta-D-glucopyranosyl)-2-arylimidazole-4(5)-carboxamides and N-(beta-D-glucopyranosyl)-4(5)-arylimidazole-2-carboxamides as glycogen phosphorylase inhibitors JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 9 SP - 1 EP - 21 PG - 21 SN - 1661-6596 DO - 10.3390/ijms25094591 UR - https://m2.mtmt.hu/api/publication/34813914 ID - 34813914 LA - English DB - MTMT ER - TY - JOUR AU - Sturniolo, I AU - Váróczy, Cs AU - Regdon, Zsolt AU - Mázló, A AU - Muzsai, Sz AU - Bácsi, A AU - Intili, G AU - Hegedűs, Csaba AU - Boothby, MR AU - Holechek, J AU - Ferraris, D AU - Schüler, H AU - Virág, László TI - PARP14 contributes to the development of the tumor-associated macrophage phenotype JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 7 SP - 1 EP - 21 PG - 21 SN - 1661-6596 DO - 10.3390/ijms25073601 UR - https://m2.mtmt.hu/api/publication/34754751 ID - 34754751 LA - English DB - MTMT ER - TY - JOUR AU - Lontay, Beáta TI - Ünnepelt a Debreceni Egyetem Orvosi Vegytani Intézete: 70 éves Dombrádi Viktor és Erdődi Ferenc JF - BIOKÉMIA: A MAGYAR BIOKÉMIAI EGYESÜLET FOLYÓIRATA J2 - BIOKÉMIA VL - XLVIII PY - 2024 IS - 1 SP - 96 EP - 100 PG - 5 SN - 0133-8455 UR - https://m2.mtmt.hu/api/publication/34747207 ID - 34747207 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Ghani, Marvi AU - Zohar, Peleg AU - Ujlaki, Gyula AU - Tóth, Melinda AU - Amsalu, Hailemariam AU - Póliska, Szilárd AU - Tar, Krisztina TI - Stable knockdown of Drp1 improves retinoic acid-BDNF-induced neuronal differentiation through global transcriptomic changes and results in reduced phosphorylation of ERK1/2 independently of DUSP1 and 6 JF - FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY J2 - FRONT CELL DEV BIOL VL - 12 PY - 2024 SP - 1 EP - 25 PG - 25 SN - 2296-634X DO - 10.3389/fcell.2024.1342741 UR - https://m2.mtmt.hu/api/publication/34738061 ID - 34738061 AB - Background: Dynamin-related protein Drp1 —a major mitochondrial fission protein— is widely distributed in the central nervous system and plays a crucial role in regulating mitochondrial dynamics, specifically mitochondrial fission and the organelle's shaping. Upregulated Drp1 function may contribute to the pathological progression of neurodegenerative diseases by dysregulating mitochondrial fission/ fusion. The study aims to investigate the effects of Drp1 on retinoic acid-BDNF-induced (RA-BDNF) neuronal differentiation and mitochondrial network reorganization in SH-SY5Y neuroblastoma cells. LA - English DB - MTMT ER - TY - JOUR AU - Thalwieser, Zsófia AU - Fonódi, Márton AU - Király, Nikolett AU - Csortos, Csilla AU - Boratkó, Anita TI - PP2A Affects Angiogenesis via Its Interaction with a Novel Phosphorylation Site of TSP1 JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 3 SP - 1844 SN - 1661-6596 DO - 10.3390/ijms25031844 UR - https://m2.mtmt.hu/api/publication/34573513 ID - 34573513 AB - Alterations in angiogenic properties play a pivotal role in the manifestation and onset of various pathologies, including vascular diseases and cancer. Thrombospondin-1 (TSP1) protein is one of the master regulators of angiogenesis. This study unveils a novel aspect of TSP1 regulation through reversible phosphorylation. The silencing of the B55α regulatory subunit of protein phosphatase 2A (PP2A) in endothelial cells led to a significant decrease in TSP1 expression. Direct interaction between TSP1 and PP2A-B55α was confirmed via various methods. Truncated TSP1 constructs were employed to identify the phosphorylation site and the responsible kinase, ultimately pinpointing PKC as the enzyme phosphorylating TSP1 on Ser93. The biological effects of B55α–TSP1 interaction were also analyzed. B55α silencing not only counteracted the increase in TSP1 expression during wound closure but also prolonged wound closure time. Although B55α silenced cells initiated tube-like structures earlier than control cells, their spheroid formation was disrupted, leading to disintegration. Cells transfected with phosphomimic TSP1 S93D exhibited smaller spheroids and reduced effectiveness in tube formation, revealing insights into the effects of TSP1 phosphorylation on angiogenic properties. In this paper, we introduce a new regulatory mechanism of angiogenesis by reversible phosphorylation on TSP1 S93 by PKC and PP2A B55α. LA - English DB - MTMT ER - TY - JOUR AU - Szeőcs, Dóra AU - Vida, Beáta AU - Petővári, Gábor AU - Póliska, Szilárd AU - Janka, Eszter Anna AU - Sipos, Adrienn AU - Uray (Davis), Karen L. AU - Sebestyén, Anna AU - Krasznai, Zoárd Tibor AU - Bay, Péter TI - Cell-free ascites from ovarian cancer patients induces Warburg metabolism and cell proliferation through TGFbeta-ERK signalling JF - GEROSCIENCE: OFFICIAL JOURNAL OF THE AMERICAN AGING ASSOCIATION (AGE) J2 - GEROSCIENCE PY - 2024 PG - 17 SN - 2509-2715 DO - 10.1007/s11357-023-01056-1 UR - https://m2.mtmt.hu/api/publication/34483936 ID - 34483936 N1 - Funding Agency and Grant Number: University of Debrecen; NKFIH [K142141, FK128387, FK146852, TKP2021-EGA-19, TKP2021-EGA-20]; Hungarian Academy of Sciences [POST-COVID2021-33, NKM2022-30]; National Research, Development and Innovation Fund of Hungary [TKP2021-EGA-19, TKP2021-EGA-20]; HUN-REN Hungarian Research Network Funding text: Open access funding provided by University of Debrecen. Our work was supported by grants from NKFIH (K142141, FK128387, FK146852, TKP2021-EGA-19, and TKP2021-EGA-20) and the Hungarian Academy of Sciences (POST-COVID2021-33, NKM2022-30). Project no. TKP2021-EGA-19 and TKP2021-EGA-20 were implemented with support provided by the National Research, Development and Innovation Fund of Hungary, financed under the TKP2021-EGA funding scheme. AS is supported by the Bolyai fellowship of the Hungarian Academy of Sciences. This project received funding from the HUN-REN Hungarian Research Network. AB - Ascites plays a key role in supporting the metastatic potential of ovarian cancer cells. Shear stress and carry-over of cancer cells by ascites flow support carcinogenesis and metastasis formation. In addition, soluble factors may participate in the procarcinogenic effects of ascites in ovarian cancer. This study aimed to determine the biological effects of cell-free ascites on carcinogenesis in ovarian cancer cells. Cell-free ascites from ovarian cancer patients (ASC) non-selectively induced cell proliferation in multiple models of ovarian cancer and untransformed primary human dermal fibroblasts. Furthermore, ASC induced a Warburg-type rearrangement of cellular metabolism in A2780 ovarian cancer cells characterized by increases in cellular oxygen consumption and glycolytic flux; increases in glycolytic flux were dominant. ASC induced mitochondrial uncoupling and fundamentally reduced fatty acid oxidation. Ascites-elicited effects were uniform among ascites specimens. ASC-elicited transcriptomic changes in A2780 ovarian cancer cells included induction of the TGF beta-ERK/MEK pathway, which plays a key role in inducing cell proliferation and oncometabolism. ASC-induced gene expression changes, as well as the overexpression of members of the TGF beta signaling system, were associated with poor survival in ovarian cancer patients. We provided evidence that the activation of the autocrine/paracrine of TGF beta signaling system may be present in bladder urothelial carcinoma and stomach adenocarcinoma. Database analysis suggests that the TGF beta system may feed forward bladder urothelial carcinoma and stomach adenocarcinoma. Soluble components of ASC support the progression of ovarian cancer. These results suggest that reducing ascites production may play an essential role in the treatment of ovarian cancer by inhibiting the progression and reducing the severity of the disease. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Emese AU - Fagyas, Miklós AU - Nagy, Béla AU - Siket, Ivetta Mányiné AU - Szőke, Blanka AU - Mártha, Lilla AU - Mahdi, Mohamed AU - Erdősi, Gábor AU - Pólik, Zsófia AU - Kappelmayer, János AU - Papp, Zoltán AU - Borbély, Attila AU - Szabó, Tamás AU - Balla, József AU - Balla, György AU - Bácsi, Attila AU - Szekanecz, Zoltán AU - Bay, Péter AU - Tóth, Attila TI - Distinct subsets of anti-pulmonary autoantibodies correlate with disease severity and survival in severe COVID-19 patients JF - GEROSCIENCE: OFFICIAL JOURNAL OF THE AMERICAN AGING ASSOCIATION (AGE) J2 - GEROSCIENCE VL - 46 PY - 2024 IS - 2 SP - 1561 EP - 1574 PG - 14 SN - 2509-2715 DO - 10.1007/s11357-023-00887-2 UR - https://m2.mtmt.hu/api/publication/34148390 ID - 34148390 AB - Autoantibodies targeting the lung tissue were identified in severe COVID-19 patients in this retrospective study. Fifty-three percent of 104 patients developed anti-pulmonary antibodies, the majority of which were IgM class, suggesting that they developed upon infection with SARS-CoV-2. Anti-pulmonary antibodies correlated with worse pulmonary function and a higher risk of multiorgan failure that was further aggravated if 3 or more autoantibody clones were simultaneously present (multi-producers). Multi-producer patients were older than the patients with less or no autoantibodies. One of the identified autoantibodies (targeting a pulmonary protein of ~ 50 kDa) associated with worse clinical outcomes, including mortality. In summary, severe COVID-19 is associated with the development of lung-specific autoantibodies, which may worsen the clinical outcome. Tissue proteome-wide tests, such as the ones applied here, can be used to detect autoimmunity in the post-COVID state to identify the cause of symptoms and to reveal a new target for treatment. LA - English DB - MTMT ER - TY - JOUR AU - Virág, László AU - Hegedűs, Csaba AU - Kovács, Katalin AU - Polgár, Zsuzsanna AU - Kókai, Endre AU - Sturniolo, Isotta AU - Demény, Máté Ágoston AU - Virág, Bernadett AU - Szabó, Éva TI - Automated high-throughput cell culture scratch assay identifies wound healing promoting and inhibiting compounds from a small compound library of redox active molecules JF - FREE RADICAL BIOLOGY AND MEDICINE J2 - FREE RADICAL BIO MED VL - 208 PY - 2023 SP - S144 EP - S144 PG - 1 SN - 0891-5849 DO - 10.1016/j.freeradbiomed.2023.10.328 UR - https://m2.mtmt.hu/api/publication/34729573 ID - 34729573 LA - English DB - MTMT ER - TY - JOUR AU - Fonódi, Márton AU - Thalwieser, Zsófia AU - Csortos, Csilla AU - Boratkó, Anita TI - TIMAP, a Regulatory Subunit of Protein Phosphatase 1, Inhibits In Vitro Neuronal Differentiation JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 24 PY - 2023 IS - 24 SN - 1661-6596 DO - 10.3390/ijms242417360 UR - https://m2.mtmt.hu/api/publication/34430018 ID - 34430018 AB - TIMAP (TGF-β-inhibited membrane associated protein) is abundant in endothelial cells, and it has been regarded as a member of the myosin phosphatase targeting protein (MYPT) family. Our workgroup previously identified several interacting protein partners of TIMAP and proved its regulatory subunit role for protein phosphatase 1 catalytic subunit (PP1c). TIMAP is also expressed in neuronal cells, but details of its function have not been studied yet. Therefore, we aimed to explore the role of TIMAP in neuronal cells, especially during differentiation. Expression of TIMAP was proved both at mRNA and protein levels in SH-SY5Y human neuroblastoma cells. Differentiation of SH-SY5Y cells was optimized and proved by the detection of neuronal differentiation markers, such as β3-tubulin, nestin and inhibitor of differentiation 1 (ID1) using qPCR and Western blot. We found downregulation of TIMAP during differentiation. In accordance with this, overexpression of recombinant TIMAP attenuated the differentiation of neuronal cells. Moreover, the subcellular localization of TIMAP has changed during differentiation as it translocated from the plasma membrane into the nucleus. The nuclear interactome of TIMAP revealed more than 50 proteins, offering the possibility to further investigate the role of TIMAP in several key physiological pathways of neuronal cells. LA - English DB - MTMT ER - TY - JOUR AU - Guti, Eliza AU - Bede, Ákos Máté AU - Váróczy, Csongor AU - Hegedűs, Csaba AU - Demény, Máté Ágoston AU - Virág, László TI - High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds JF - JOVE-JOURNAL OF VISUALIZED EXPERIMENTS J2 - JOVE-J VIS EXP PY - 2023 IS - 198 SP - 1 EP - 12 PG - 12 SN - 1940-087X DO - 10.3791/64485 UR - https://m2.mtmt.hu/api/publication/34108111 ID - 34108111 LA - English DB - MTMT ER -