TY - JOUR AU - Szabó, Dávid Ruben AU - Felinger, Attila AU - Horváth, Krisztián AU - Gáspár, Attila TI - Mathematical Modeling of Taylor-Aris Dispersion-Assisted Mass Spectrometry for the Study of Operating Conditions JF - TALANTA J2 - TALANTA VL - 296 PY - 2026 PG - 7 SN - 0039-9140 DO - 10.1016/j.talanta.2025.128435 UR - https://m2.mtmt.hu/api/publication/36183982 ID - 36183982 LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Balázs István AU - Bazeli, Bahar AU - Janssens, Annelies AU - Lisztes, Erika AU - Racskó, Márk AU - Kelemen, Balázs AU - Herczeg, Mihály AU - Nagy, Tamás Milán AU - E Kövér, Katalin AU - Mitra, Argha AU - Borics, Attila AU - Bíró, Tamás AU - Voets, Thomas TI - Direct modulation of TRPM8 ion channels by rapamycin and analog macrolide immunosuppressants JF - ELIFE J2 - ELIFE VL - 13 PY - 2025 PG - 33 SN - 2050-084X DO - 10.7554/eLife.97341.2 UR - https://m2.mtmt.hu/api/publication/34722559 ID - 34722559 AB - Rapamycin (sirolimus), a macrolide compound isolated from the bacterium Streptomyces hygroscopicus, is widely used as oral medication for the prevention of transplant rejection and the treatment of lymphangioleiomyomatosis. It is also incorporated in coronary stent coatings to prevent restenosis and in topical preparations for the treatment of skin disorders. Rapamycin’s in vivo activities are generally ascribed to its binding to the protein FKBP12, leading to potent inhibition of the mechanistic target of rapamycin kinase (mTOR) by the FKBP12-rapamycin complex. The specific rapamycin-induced interaction between domains from mTOR and FKBP12 is also frequently employed in cell biological research, for rapid chemically-induced protein dimerization strategies. Here we show that rapamycin activates TRPM8, a cation channel expressed in sensory nerve endings that serves as the primary cold sensor in mammals. Using a combination of electrophysiology, Saturation Transfer Triple-Difference (STTD) NMR spectroscopy and molecular docking-based targeted mutagenesis, we demonstrate that rapamycin directly binds to TRPM8. We identify a rapamycin-binding site in the groove between voltage sensor-like domain and the pore domain, distinct from the interaction sites of cooling agents and known TRPM8 agonists menthol and icilin. Related macrolide immunosuppressants act as partial TRPM8 agonists, competing with rapamycin for the same binding site. These findings identify a novel molecular target for rapamycin and provide new insights into the mechanisms of TRPM8 activation, which may assist in the development of therapies targeting this ion channel. Moreover, our findings also indicate that caution is needed when using molecular approaches based on rapamycin-induced dimerization to study ion channel regulation. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Dávid Ruben AU - Szabo, Peter AU - Gáspár, Attila TI - Taylor-Aris dispersion assisted ESI-MS applied in a simple flow injection system JF - MICROCHEMICAL JOURNAL J2 - MICROCHEM J VL - 209 PY - 2025 PG - 6 SN - 0026-265X DO - 10.1016/j.microc.2025.112785 UR - https://m2.mtmt.hu/api/publication/35708306 ID - 35708306 N1 - Export Date: 10 February 2025; Cited By: 0; Correspondence Address: A. Gaspar; email: gaspar@science.unideb.hu; CODEN: MICJA AB - This work demonstrates that the recently introduced TADA-MS method (originally designed in a CE instrument) can be applied in a simple, low-pressure flow injection system, which is more widely accessible in an average MS analytical laboratory. An essential part of the system is an internal sample chamber micro-scale injector. A sample volume of 0.5 μL was mobilized by a syringe pump. The analytical applicability of the proposed system was tested by analyzing concanavalin A protein under denaturing and native conditions. The obtained results demonstrated that in the proposed low-pressure flow injection TADA-MS system the capillaries of both 50 μm and 100 μm i.d., and both the conventional ESI interface and the coaxial sheath-liquid CE-ESI interface can be efficiently applied – the obtained MS spectra were similar. LA - English DB - MTMT ER - TY - JOUR AU - Csupász-Szabó, Hanna Judit AU - Döncző, Boglárka AU - Szarka, Máté AU - Daróczi, Lajos AU - Lázár, István TI - Thermal Reverse-Engineered Synthesis and Catalytic Activity of Nanogold-Containing Silica Aerogels JF - GELS (BASEL) J2 - GELS-BASEL VL - 11 PY - 2025 IS - 2 PG - 19 SN - 2310-2861 DO - 10.3390/gels11020087 UR - https://m2.mtmt.hu/api/publication/35712556 ID - 35712556 N1 - Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem tér 1, Debrecen, 4032, Hungary HUN-REN Institute for Nuclear Research (ATOMKI), Bem tér 18/c, Debrecen, 4026, Hungary Department of Solid State Physics, University of Debrecen, Bem tér 18/b, Debrecen, 4026, Hungary Export Date: 12 March 2025; Correspondence Address: I. Lázár; Department of Inorganic and Analytical Chemistry, University of Debrecen, Debrecen, Egyetem tér 1, 4032, Hungary; email: lazar@science.unideb.hu AB - Silica aerogels are extensively used as catalyst supports due to their mesoporous structure and chemical inertness. In this study, SiO2–AuNP aerogels containing gold nanoparticles (AuNPs) were synthesized using the sol-gel method followed by supercritical CO2 drying. The inclusion of polyvinyl pyrrolidone (PVP) as a stabilizing agent preserved the gold particle sizes during the gelation process. In contrast, aerogels synthesized without PVP contained enlarged AuNP aggregates, resulting in a shift in the plasmon resonance color from red to bluish or blue–grey. Thermal treatment of these bluish-colored aerogels at high temperatures restored their red coloration, visually indicating the breakdown of large gold clusters into individual nanoparticles. Both types of aerogels were characterized using SEM, TEM, 3D optical microscopy, UV–vis and ATR-IR spectroscopy, and N2 porosimetry, with their properties analyzed as a function of annealing temperature. Their catalytic activity was evaluated through the reduction of 4-nitrophenol with sodium borohydride, and both aerogel types demonstrated catalytic activity. This thermal conversion of large clusters into individual nanoparticles within an aerogel matrix introduces a new and promising approach for creating catalytically active nanogold-containing aerogel catalysts. LA - English DB - MTMT ER - TY - GEN AU - Bárány, Zsolt Béla AU - Dóbéné, Cserjés Edit AU - Lente, Gábor AU - Márkus, Teréz AU - Musza, Katalin AU - Petri, Judit AU - Tóth, Imre AU - Várnagy, Katalin ED - Ősz, Katalin TI - 57. Irinyi János Középiskolai Kémiaverseny, I. forduló - Feladatok és Megoldások, Pontozási Útmutató (2025) PY - 2025 UR - https://m2.mtmt.hu/api/publication/35718417 ID - 35718417 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Kovács, Fruzsina AU - Jakab, Ágnes AU - Balla , Noémi AU - Tóth, Zoltán AU - Balázsi, Dávid AU - Forgács, Lajos AU - Harmath, Andrea AU - Bozó, Aliz AU - Ragyák, Ágota AU - Majoros, László AU - Kovács, Renátó TI - A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicans JF - BIOFILM J2 - Biofilm VL - 9 PY - 2025 PG - 12 SN - 2590-2075 DO - 10.1016/j.bioflm.2025.100259 UR - https://m2.mtmt.hu/api/publication/35729613 ID - 35729613 LA - English DB - MTMT ER - TY - JOUR AU - Barbosa, Barbara Marinho AU - Pires, Daniele C. AU - Galvácsi, Antal Tamás AU - Gaudard, Anna Julia AU - Flores, Leona S. AU - Sanchez-Lopez, Carolina AU - Correa, Charlane C. AU - Cukierman, Daphne S. AU - Hureau, Christelle AU - Kállay, Csilla AU - Pereira, Marcos D. AU - Quintanar, Liliana AU - Rey, Nicolas A. TI - A New Cu2+-Binding 1,3-Benzodioxole-Contaning N-Acylhydrazone Protects Saccharomyces cerevisiae Cells from Oxidative Stress JF - CHEMISTRYSELECT J2 - CHEMISTRYSELECT VL - 10 PY - 2025 IS - 6 PG - 15 SN - 2365-6549 DO - 10.1002/slct.202405195 UR - https://m2.mtmt.hu/api/publication/35767451 ID - 35767451 N1 - Funding Agency and Grant Number: CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brazil); FAPERJ (Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro, Brazil); FAPERJ; CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil); CAPES-COFECUB project [88,881.878977/2023-01]; [001] Funding text: BMB and DCP acknowledge CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brazil), for the scholarships awarded (finance code 001). MDP is indebted to FAPERJ (Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro, Brazil). NAR thanks FAPERJ and CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil) for the research fellowships. This work is part of an ongoing CAPES-COFECUB project (88,881.878977/2023-01) coordinated by Professors Nicolas A. Rey (Brazil) and Christelle Hureau (France). AB - Copper dyshomeostasis is related to an increase in oxidative stress that, in turn, has been associated with a myriad of diseases and physiological aging. Based on this, the study of compounds with moderate affinity for this biometal is of interest. In this scenario, a new, highly stable, and non-toxic (assessed in S. cerevisiae) 1-methylimidazole-containing N-acylhydrazone, HX1Diox, is synthesized and characterized, including by XRD. The compound forms complexes of 1:1 and 1:2 copper(II)-to-ligand stoichiometries in solution, while only the non-charged 1:2 compound, [Cu(X1Diox)2], is isolated in the solid state. In this case, each hydrazone unit is fully deprotonated and coordinates, in the iminolate X1Diox- form, as an N2O- meridional tridentate ligand. The soluble cationic species [Cu(X1Diox)(OH2)x]+ and [Cu(HX1Diox)(X1Diox)]+ are detected solely in solution, and stability constants of 12.49(4) and 28.42(6) are determined, respectively, for each of them. Interestingly, HX1Diox shows promising activity as a scavenger of superoxide radical anions, assessed in vitro, and eukaryotic cells by using wild-type and SOD1-deficient strains of S. cerevisiae yeast. Altogether, these results point to a promising copper interaction profile of HX1Diox, which, coupled with its favorable antioxidant profile, encourages further studies in the context of ROS-related diseases. LA - English DB - MTMT ER - TY - JOUR AU - Anwar, Armin AU - Mezbah Uddin, A. H.M. AU - Hasan, Md. Siddiq AU - Parvez, Md Sohel AU - Sipos, Bianka AU - Bibi, Dina AU - Sajtos, Zsófi AU - Tóthmérész, Béla AU - Magura, Tibor AU - Simon, Edina TI - Assessment of anthropogenic activities impact based on metals in soil and tree leaves along roadside in Bangladesh JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 15 PY - 2025 IS - 1 PG - 14 SN - 2045-2322 DO - 10.1038/s41598-025-91683-9 UR - https://m2.mtmt.hu/api/publication/35799281 ID - 35799281 AB - The assessment of heavy metal pollution in leaves and soils is essential in environmental health monitoring. We assessed the effect of anthropogenic activities along an industrial–urban–rural gradient in the roadside soil and tree leaves of Bangladesh based on their metal concentration. The concentrations of Pb, Cr, Ni, and Cd were analysed in leaves of Ficus benghalensis , Swietenia macrophylla , and Polyalthia longifolia and topsoil samples using ICP-OES. We used pollution indices to quantify the impact of contaminants on ecosystems. Our findings indicated that there were higher metal concentrations, especially Cd and Pb, in soil and leaves in close proximity to locations with substantial traffic and industrial activities. Igeo index indicated moderately to strongly polluted areas by Cd. The contamination factor (CF) for Cd indicated a severe contamination, while moderately contamination show by Cr, Pb, and Ni. The value of the ecological risk factor was below 40 for Cr, Ni, and Pb; thus, negligible ecological risks were considered for these elements. But, the ecological risk assessment indices showed that Cd poses high ecological risks in soil and leaves across all study areas. Bioaccumulation analysis indicated substantial heavy metal absorption in tree foliage, with F. benghalensis and P. longifolia exhibiting elevated Pb accumulation in urban environments. The PCA indicated that Cd, Cr, Ni, and Pb mostly derived from anthropogenic sources in leaves and soil samples, too. Our results demonstrated that F. benghalensis , S. macrophylla , and P. longifolia are good indicator to assess the effect of anthropogenic activities on the terrestrial ecosystem. Our results also indicated the critical need for environmental monitoring and mitigation techniques in urban and industrial zones to reduce heavy metal pollution in roadside ecosystems. LA - English DB - MTMT ER - TY - JOUR AU - Grattoni, Elena AU - Travagin, Fabio AU - Kálmán, Ferenc Krisztián AU - Baranyai, Zsolt AU - Negri, Roberto AU - Carniato, Fabio AU - Giovenzana, Giovanni B. AU - Platas-Iglesias, Carlos AU - Botta, Mauro TI - Evaluation of structurally related acyclic ligands OBETA, EHDTA, and EGTA for stable Mn2+ complex formation JF - DALTON TRANSACTIONS J2 - DALTON TRANSACT VL - 54 PY - 2025 IS - 1 SP - 376 EP - 388 PG - 13 SN - 1477-9226 DO - 10.1039/d4dt02761b UR - https://m2.mtmt.hu/api/publication/35805561 ID - 35805561 N1 - Funding Agency and Grant Number: MUR - M4C2 1.5 of PNRR [ECS00000036]; Ministerio de Ciencia e Innovacion [PID2022-138335NB-I00]; Xunta de Galicia [ED431C 2023/33]; Centro de Supercomputacion de Galicia (CESGA); Hungarian National Research, Development and Innovation Office [FK-134551]; New National Excellence Program [UNKP-22-5]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences Funding text: This publication is part of the project NODES which has received funding from the MUR - M4C2 1.5 of PNRR with grant agreement no. ECS00000036 (M. B., G. B. G., F. T.). C. P.-I. thanks Ministerio de Ciencia e Innovacion (PID2022-138335NB-I00) and Xunta de Galicia (ED431C 2023/33) for generous financial support, as well as Centro de Supercomputacion de Galicia (CESGA) for providing the computing facilities. The research was also partially funded by the Hungarian National Research, Development and Innovation Office (FK-134551) project, the New National Excellence Program UNKP-22-5 (F. K. K.). F. K. K. acknowledges financial support of the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. AB - In recent years, significant research efforts have been dedicated to finding efficient and safe alternatives to the currently used gadolinium (Gd)-based MRI contrast agents. Among the most explored alternatives are paramagnetic chelates of the Earth-abundant Mn2+, which form a prominent class of metal complexes. The design of Mn2+ complexes with enhanced relaxation properties and improved safety profiles hinges on a delicate balance between thermodynamic and kinetic stability, as well as the presence of coordinated water molecules. In this study, we present a comprehensive investigation into the coordination chemistry of three structurally related polyetheraminocarboxylic chelating agents. Our aim is to elucidate the structural features, paramagnetic properties, and thermodynamic and kinetic inertness of the corresponding Mn2+ complexes. The most significant finding is the considerable difference in the dissociation rates of the complexes, with the octadentate EGTA complex being the most labile. The observed dissociation rates correlate well with the nitrogen inversion dynamics, as assessed through NMR spectral analysis of the analogous Zn2+ complexes. LA - English DB - MTMT ER - TY - JOUR AU - Parvez, Md Sohel AU - Imdadul Hoque, Md. AU - Nurul Momen Siddique, Md. AU - Rahman, Mohammad AU - Baranyai, Edina AU - Sajtos, Zsófi AU - Döncző, Boglárka AU - Aib, Haithem AU - Abdul Kader, Md. AU - Simon, Edina AU - Czédli, Herta TI - Elemental analysis in the scales of commercially important coastal fishes and their connections with fish feeding habits and habitats JF - FRONTIERS IN MARINE SCIENCE J2 - FRONT MAR SCI VL - 12 PY - 2025 PG - 13 SN - 2296-7745 DO - 10.3389/fmars.2025.1546313 UR - https://m2.mtmt.hu/api/publication/35860389 ID - 35860389 N1 - Doctoral School of Biology and Environmental Sciences, University of Debrecen, Debrecen, Hungary Department of Hydrobiology, University of Debrecen, Debrecen, Hungary Department of Oceanography, Noakhali Science and Technology University, Noakhali, Bangladesh Department of Urdu, University of Dhaka, Dhaka, Bangladesh Local Government Initiatives on Climate Change (LoGIC), United Nations Development Programme, Bhola, Bangladesh Institute of Marine Sciences, University of Chittagong, Chattogram, Bangladesh Department of Inorganic and Analytical Chemistry, University of Debrecen, Debrecen, Hungary HUN-REN Institute for Nuclear Research (ATOMKI), Debrecen, Hungary Bangladesh Space Research and Remote Sensing Organization (SPARRSO), Dhaka, Bangladesh Department of Ecology, University of Debrecen, Debrecen, Hungary HUN-REN–UD Anthropocene Ecology Research Group, Debrecen, Hungary Department of Civil Engineering, University of Debrecen, Debrecen, Hungary Export Date: 23 April 2025; Correspondence Address: H. Czédli; Department of Civil Engineering, University of Debrecen, Debrecen, Hungary; email: herta.czedli@eng.unideb.hu LA - English DB - MTMT ER -