TY - JOUR AU - Vishwakarma, Gayatri AU - Andrási, Melinda AU - Szabó, Dávid Ruben AU - Hajdu, Péter Béla AU - Petrilla, Vladimir AU - Petrillová, Monika AU - Legath, Jaroslav AU - Gáspár, Attila TI - Analysis of intact venom proteins with capillary zone electrophoresis - mass spectrometry JF - MICROCHEMICAL JOURNAL J2 - MICROCHEM J VL - 200 PY - 2024 PG - 8 SN - 0026-265X DO - 10.1016/j.microc.2024.110290 UR - https://m2.mtmt.hu/api/publication/34774200 ID - 34774200 N1 - Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem ter 1., Debrecen, 4032, Hungary Department of Dental Biochemistry and Department of Biophysics and Cell Biology, University of Debrecen, Egyetem tér 1., Debrecen, 4032, Hungary Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy, Košice, Slovakia Zoological Department, Zoological Garden Košice, Košice-Kavečany, Slovakia Department of General Competencies, University of Veterinary Medicine and Pharmacy, Košice, Slovakia Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Košice, Slovakia Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Rzeszow, Poland Export Date: 6 April 2024 CODEN: MICJA Correspondence Address: Gaspar, A.; Department of Inorganic and Analytical Chemistry, Egyetem ter 1., Hungary; email: gaspar@science.unideb.hu Funding details: Ministerstvo školstva, vedy, výskumu a športu Slovenskej republiky Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIH, K142134, K128525, 2022_526066, APVV - 22-0101 Funding text 1: The authors acknowledge the financial support provided for this project by the National Research, Development and Innovation Office, Hungary (K142134 (AG), K128525 (HP)), Stipendium Hungaricum (#2022_526066) and by the project APVV-22-0101 (Slovak Research and Development Agency Departmental Organization of the Ministry of Education, Science, Research and Sports of the Slovak Republic). LA - English DB - MTMT ER - TY - JOUR AU - Ecsédi, Bertold AU - Forgács, Attila AU - Balogh, Zoltán AU - Fábián, István AU - Kalmár, József TI - Hydration and wetting mechanism of borosilicate – Polyvinyl alcohol (PVA) hybrid aerogels of potential bioactivity JF - JOURNAL OF MOLECULAR LIQUIDS J2 - J MOL LIQ VL - 401 PY - 2024 PG - 8 SN - 0167-7322 DO - 10.1016/j.molliq.2024.124605 UR - https://m2.mtmt.hu/api/publication/34763386 ID - 34763386 LA - English DB - MTMT ER - TY - JOUR AU - Simon, Fruzsina AU - Fábián, István AU - Szabó, Mária TI - Oxidation of branched chain amino acids by HOCl: kinetics and mechanism JF - JOURNAL OF HAZARDOUS MATERIALS J2 - J HAZARD MATER VL - 470 PY - 2024 SP - 134145 SN - 0304-3894 DO - 10.1016/j.jhazmat.2024.134145 UR - https://m2.mtmt.hu/api/publication/34763028 ID - 34763028 LA - English DB - MTMT ER - TY - JOUR AU - Dibello, Estefanía AU - Oddone, Natalia AU - Franco, Jaime AU - Tóthné Illyés, Tünde Zita AU - Medeiros, Andrea AU - Kiss, Attila AU - Hőgye, Fanni AU - E Kövér, Katalin AU - Szilágyi, László AU - Comini, Marcelo A. TI - Selenosugars targeting the infective stage of Trypanosoma brucei with high selectivity JF - INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE J2 - INT J PARASITOL-DRUG VL - 24 PY - 2024 PG - 6 SN - 2211-3207 DO - 10.1016/j.ijpddr.2024.100529 UR - https://m2.mtmt.hu/api/publication/34743720 ID - 34743720 AB - Earlier evidences showed that diglycosyl diselenides are active against the infective stage of African trypanosomes (top hits IC50 0.5 and 1.5 μM) but poorly selective (selectivity index <10). Here we extended the study to 33 new seleno-glycoconjugates with the aim to improve potency and selectivity. Three selenoglycosides and three glycosyl selenenylsulfides displayed IC50 against bloodstream Trypanosoma brucei in the sub-μM range (IC50 0.35–0.77 μM) and four of them showed an improved selectivity (selectivity index >38-folds vs. murine and human macrohages). For the glycosyl selenylsulfides, the anti-trypanosomal activity was not significantly influenced by the nature of the moiety attached to the sulfur atom. Except for a quinoline-, and to a minor extent a nitro-derivative, the most selective hits induced a rapid (within 60 min) and marked perturbation of the LMWTredox homeostasis. The formation of selenenylsulfide glycoconjugates with free thiols has been identified as a potential mechanism involved in this process. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Balázs István AU - Bahar, Bazeli AU - Annelies, Janssens AU - Lisztes, Erika AU - Racskó, Márk AU - Kelemen, Balázs AU - Herczeg, Mihály AU - Nagy, Tamás Milán AU - E Kövér, Katalin AU - Argha, Mitra AU - Attila, Borics AU - Bíró, Tamás AU - Thomas, Voets TI - Direct modulation of TRPM8 ion channels by rapamycin and analog macrolide immunosuppressants JF - ELIFE J2 - ELIFE PY - 2024 SN - 2050-084X UR - https://m2.mtmt.hu/api/publication/34722559 ID - 34722559 N1 - preprint DOI-ja: 10.1101/2024.02.01.578392 LA - English DB - MTMT ER - TY - JOUR AU - Balogh, Zoltán AU - Kalmár, József AU - Gommes, Cedric J. TI - Wetting of alginate aerogels, from mesoporous solids to hydrogels: a small-angle scattering analysis JF - JOURNAL OF APPLIED CRYSTALLOGRAPHY J2 - J APPL CRYSTALLOGR VL - 57 PY - 2024 IS - 2 SP - 369 EP - 379 PG - 11 SN - 0021-8898 DO - 10.1107/S1600576724001705 UR - https://m2.mtmt.hu/api/publication/34655160 ID - 34655160 N1 - Accepted on 20-Febr-2024. Published on-line on 21-March-2024. LA - English DB - MTMT ER - TY - JOUR AU - Szabó, Dávid Ruben AU - Nagy, Cynthia Nóra AU - Gáspár, Attila TI - Direct Injection Electrospray Ionization Mass Spectrometry (ESI-MS) Analysis of Proteins with High Matrix Content:Utilizing Taylor–Aris Dispersion JF - ANGEWANDTE CHEMIE-INTERNATIONAL EDITION J2 - ANGEW CHEM INT EDIT VL - 63 PY - 2024 IS - 15 PG - 8 SN - 1433-7851 DO - 10.1002/anie.202318225 UR - https://m2.mtmt.hu/api/publication/34654115 ID - 34654115 N1 - Export Date: 21 February 2024; Cited By: 0; Correspondence Address: A. Gaspar; Department of Inorganic and Analytical Chemistry, Institute of Chemistry, University of Debrecen, Debrecen, 4032, Hungary; email: gaspar@science.unideb.hu; CODEN: ACIEF AB - This is the first work demonstrating the utility of the Taylor–Aris (TA) dispersion in avoiding serious interference issues commonly occurring in the electrospray ionization-mass spectrometric (ESI-MS) determination of therapeutic protein pharmaceuticals undergoing no pre-separation or sample purification. It was also pointed out that the TA dispersion conditions and its analytical utilization for proteomics can be easily accomplished in a commercial CE-MS instrument. In the proposed Taylor–Aris dispersion-assisted mass spectrometry (TADA-MS) analysis 0.5 μL sample is injected into a 65 cm long 50 μm i.d. capillary and pumped with 1 bar toward the MS. The procedure is efficient for the direct injection analysis of components having low diffusion coefficients (proteins) that are present in complex matrices of small organic and inorganic compounds. © 2024 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH. LA - English DB - MTMT ER - TY - JOUR AU - Duong, Thoa AU - Vivero-Lopez, Maria AU - Ardao, Inés AU - Alvarez-Lorenzo, Carmen AU - Forgács, Attila AU - Kalmár, József AU - García-González, Carlos A. TI - Alginate aerogels by spray gelation for enhanced pulmonary delivery and solubilization of beclomethasone dipropionate JF - CHEMICAL ENGINEERING JOURNAL J2 - CHEM ENG J VL - 485 PY - 2024 PG - 15 SN - 1385-8947 DO - 10.1016/j.cej.2024.149849 UR - https://m2.mtmt.hu/api/publication/34629003 ID - 34629003 N1 - Accepted on 18-February-2024. Published online on 20-February-2024. LA - English DB - MTMT ER - TY - JOUR AU - Székely, Enikő AU - Molnár, Mariann AU - Lihi, Norbert AU - Várnagy, Katalin TI - Characterization of Copper(II) and Zinc(II) Complexes of Peptides Mimicking the CuZnSOD Enzyme JF - MOLECULES J2 - MOLECULES VL - 29 PY - 2024 IS - 4 PG - 27 SN - 1420-3049 DO - 10.3390/molecules29040795 UR - https://m2.mtmt.hu/api/publication/34577813 ID - 34577813 AB - Antimicrobial peptides are short cationic peptides that are present on biological surfaces susceptible to infection, and they play an important role in innate immunity. These peptides, like other compounds with antimicrobial activity, often have significant superoxide dismutase (SOD) activity. One direction of our research is the characterization of peptides modeling the CuZnSOD enzyme and the determination of their biological activity, and these results may contribute to the development of novel antimicrobial peptides. In the framework of this research, we have synthesized 10, 15, and 16-membered model peptides containing the amino acid sequence corresponding to the Cu(II) and Zn(II) binding sites of the CuZnSOD enzyme, namely the Zn(II)-binding HVGD sequence (80–83. fragments), the Cu(II)-binding sequence HVH (fragments 46–48), and the histidine (His63), which links the two metal ions as an imidazolate bridge: Ac-FHVHEGPHFN-NH2 (L1(10)), Ac-FHVHAGPHFNGGHVG-NH2 (L2(15)), and Ac-FHVHEGPHFNGGHVGD-NH2 (L3(16)). pH-potentiometric, UV-Vis-, and CD-spectroscopy studies of the Cu(II), Zn(II), and Cu(II)-Zn(II) mixed complexes of these peptides were performed, and the SOD activity of the complexes was determined. The binding sites preferred by Cu(II) and Zn(II) were identified by means of CD-spectroscopy. From the results obtained for these systems, it can be concluded that in equimolar solution, the –(NGG)HVGD- sequence of the peptides is the preferred binding site for copper(II) ion. However, in the presence of both metal ions, according to the native enzyme, the -HVGD- sequence offers the main binding site for Zn(II), while the majority of Cu(II) binds to the -FHVH- sequence. Based on the SOD activity assays, complexes of the 15- and 16-membered peptide have a significant SOD activity. Although this activity is smaller than that of the native CuZnSOD enzyme, the complexes showed better performance in the degradation of superoxide anion than other SOD mimics. Thus, the incorporation of specific amino acid sequences mimicking the CuZnSOD enzyme increases the efficiency of model systems in the catalytic decomposition of superoxide anion. LA - English DB - MTMT ER - TY - JOUR AU - Balogh, Zoltán AU - Len, Adél AU - Baksa, Viktória AU - Krajnc, Andraž AU - Herman, Petra AU - Szemán-Nagy, Gábor AU - Czigány, Zsolt AU - Fábián, István AU - Kalmár, József AU - Dudás, Zoltán Imre TI - Nanoscale structural characteristics and in vitro bioactivity of borosilicate – polyvinyl alcohol (PVA) hybrid aerogels for bone regeneration JF - ACS APPLIED NANO MATERIALS J2 - ACS APPL NANO MATER VL - 7 PY - 2024 IS - 4 SP - 4092 EP - 4102 PG - 11 SN - 2574-0970 DO - 10.1021/acsanm.3c05668 UR - https://m2.mtmt.hu/api/publication/34538335 ID - 34538335 LA - English DB - MTMT ER -