TY - JOUR AU - Telek, Elek AU - Ujfalusi, Zoltán AU - Nyitrai, Miklós AU - Bogner, Péter AU - Lukács, András Szilárd AU - Németh, Timea AU - Hild, Gabriella AU - Hild, Gábor TI - Deconvolution Analysis of the Non-Ionic Iomeprol, Iobitridol and Iodixanol Contrast Media-Treated Human Whole Blood Thermograms: A Comparative Study JF - DIAGNOSTICS J2 - DIAGNOSTICS VL - 13 PY - 2023 IS - 15 PG - 18 SN - 2075-4418 DO - 10.3390/diagnostics13152523 UR - https://m2.mtmt.hu/api/publication/34095179 ID - 34095179 N1 - Department of Biophysics, Medical School, University of Pécs, Szigeti Str. 12, Pécs, H-7624, Hungary Szentágothai Research Center, Ifjúság Str. 34, Pécs, H-7624, Hungary MTA-PTE Nuclear-Mitochondrial Interactions Research Group, Szigeti Str. 12, Pécs, H-7624, Hungary Department of Medical Imaging, Clinical Centre, University of Pécs, Ifjúság Str. 13, Pécs, H-7624, Hungary Languages for Biomedical Purposes and Communication, Medical School, University of Pécs, Szigeti Str. 12, Pécs, H-7624, Hungary Export Date: 22 August 2023 Correspondence Address: Hild, G.; Department of Biophysics, Szigeti Str. 12, Hungary; email: gabriella.hild@aok.pte.hu AB - To study the effect of non-ionic contrast media on anticoagulated and non-anticoagulated human whole blood samples, calorimetric measurements were performed. The anticoagulated plasma showed the greatest fall in the total ΔH after Iodixanol treatment. The plasma-free erythrocytes revealed a pronounced shift in the Tmax and a decrease in the ΔH of hemoglobin and transferrin. The total ΔH of Iodixanol treatment showed the highest decline, while Iomeprol and Iobitridol had fewer adverse effects. Similarly, the non-anticoagulated samples revealed a decrease both in the Tmax and the ΔH of albumin and immunoglobulin-specific transitions. The total ΔH showed that Iodixanol had more influence on the serum. The serum-free erythrocyte samples resulted in a significant drop in the Tmax of erythrocyte and transferrin (~5–6 °C). The ΔH of deconvolved hemoglobin and transferrin decreased considerably; however, the ΔH of albumin increased. Surprisingly, compared to Iomeprol and Iobitridol treatments, the total ΔH of Iodixanol was less pronounced in the non-anticoagulated erythrocyte samples. In sum, each non-ionic contrast medium affected the thermal stability of anticoagulated and non-anticoagulated erythrocyte proteins. Interestingly, Iodixanol treatment caused more significant effects. These findings suggest that conformational changes in blood components can occur, which can potentially lead to the increased prevalence of cardiovascular dysfunctions and blood clotting. LA - English DB - MTMT ER - TY - JOUR AU - Longauer, Beáta AU - Bódis, Emőke AU - Lukács, András Szilárd AU - Barkó, Szilvia AU - Nyitrai, Miklós TI - Solubility and Thermal Stability of Thermotoga maritima MreB JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 24 PG - 13 SN - 1661-6596 DO - 10.3390/ijms232416044 UR - https://m2.mtmt.hu/api/publication/33366469 ID - 33366469 AB - The basis of MreB research is the study of the MreB protein from the Thermotoga maritima species, since it was the first one whose crystal structure was described. Since MreB proteins from different bacterial species show different polymerisation properties in terms of nucleotide and salt dependence, we conducted our research in this direction. For this, we performed measurements based on tryptophan emission, which were supplemented with temperature-dependent and chemical denaturation experiments. The role of nucleotide binding was studied through the fluorescent analogue TNP-ATP. These experiments show that Thermotoga maritima MreB is stabilised in the presence of low salt buffer and ATP. In the course of our work, we developed a new expression and purification procedure that allows us to obtain a large amount of pure, functional protein. LA - English DB - MTMT ER - TY - JOUR AU - Kőszegi, Balázs AU - Balogh, Gábor AU - Berente, Zoltán AU - Vranesics, Anett AU - Pollák, Edit AU - Molnár, László AU - Takátsy, Anikó AU - Poór, Viktória AU - Wahr, Mátyás AU - Antus, Csenge Petra AU - Erős, Krisztián AU - Vigh, László AU - Gallyas, Ferenc AU - Péter, Mária AU - Veres, Balázs TI - Remodeling of Liver and Plasma Lipidomes in Mice Lacking Cyclophilin D JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 23 PY - 2022 IS - 19 PG - 16 SN - 1661-6596 DO - 10.3390/ijms231911274 UR - https://m2.mtmt.hu/api/publication/33154711 ID - 33154711 N1 - * Megosztott szerzőség AB - In recent years, several studies aimed to investigate the metabolic effects of non-functioning or absent cyclophilin D (CypD), a crucial regulatory component of mitochondrial permeability transition pores. It has been reported that the lack of CypD affects glucose and lipid metabolism. However, the findings are controversial regarding the metabolic pathways involved, and most reports describe the effect of a high-fat diet on metabolism. We performed a lipidomic analysis of plasma and liver samples of CypD-/- and wild-type (WT) mice to reveal the lipid-specific alterations resulting from the absence of CypD. In the CypD-/- mice compared to the WT animals, we found a significant change in 52% and 47% of the measured 225 and 201 lipid species in liver and plasma samples, respectively. The higher total lipid content detected in these tissues was not accompanied by abdominal fat accumulation assessed by nuclear magnetic resonance imaging. We also documented characteristic changes in the lipid composition of the liver and plasma as a result of CypD ablation with the relative increase in polyunsaturated membrane lipid species. In addition, we did not observe remarkable differences in the lipid distribution of hepatocytes using histochemistry, but we found characteristic changes in the hepatocyte ultrastructure in CypD-/- animals using electron microscopy. Our results highlight the possible long-term effects of CypD inhibition as a novel therapeutic consideration for various diseases. LA - English DB - MTMT ER - TY - JOUR AU - Telek, Elek AU - Karadi, Kristof AU - Kardos, József AU - Kengyel, Andras AU - Fekete, Zsuzsanna AU - Halasz, Henriett AU - Nyitrai, Miklos AU - Bugyi, Beata AU - Lukács, András Szilárd TI - Conformational dynamics and functional characterization of the C-terminal tail of Myosin 16 JF - BIOPHYSICAL JOURNAL J2 - BIOPHYS J VL - 121 PY - 2022 IS - 3 SP - 181A EP - 181A PG - 1 SN - 0006-3495 UR - https://m2.mtmt.hu/api/publication/32760182 ID - 32760182 LA - English DB - MTMT ER - TY - JOUR AU - Ujfalusi, Zoltán AU - Telek, Elek AU - Nyitrai, Miklós AU - Bogner, Péter AU - Rostás, Tamás AU - Hild, Gabriella AU - Trif, László AU - Hild, Gábor TI - The effect of Iodixanol on the thermodynamic properties of blood components JF - THERMOCHIMICA ACTA J2 - THERMOCHIM ACTA VL - 710 PY - 2022 PG - 7 SN - 0040-6031 DO - 10.1016/j.tca.2022.179165 UR - https://m2.mtmt.hu/api/publication/32653646 ID - 32653646 LA - English DB - MTMT ER - TY - JOUR AU - Isbera, Mostafa AU - Bognár, Balázs AU - Gallyas, Ferenc AU - Bényei, Attila Csaba AU - Jekő, József AU - Kálai, Tamás TI - Syntheses and study of a pyrroline nitroxide condensed phospholene oxide and a pyrroline nitroxide with diphenylphosphino moiety JF - PHOSPHORUS SULFUR AND SILICON AND THE RELATED ELEMENTS J2 - PHOSPHOR SULFUR SIL REL ELEM VL - 197 PY - 2022 IS - 5-6 SP - 515 EP - 517 PG - 3 SN - 1042-6507 DO - 10.1080/10426507.2021.1989690 UR - https://m2.mtmt.hu/api/publication/32466727 ID - 32466727 N1 - Export Date: 6 March 2024 CODEN: PSSLE Correspondence Address: Kálai, T.; Faculty of Pharmacy, Honvéd st. 1, Hungary; email: tamas.kalai@aok.pte.hu AB - The reaction of a diene nitroxide precursor with dichlorophenylphosphine in a McCormac procedure afforded pyrroline nitroxide condensed phospholen oxide. Lithiation of the protected 3-bromo-pyrroline nitroxide followed by treatment with chlorodiphenylphosphine after deprotection afforded (1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)diphenylphosphine oxide, and after reduction, (1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)diphenylphosphine was realized, which was also supported by X-ray single crystal diffraction measurements. This pyrroline diphenylphosphine derivative was converted to hexadecylphosphonium salt, which was more effective antineoplastic agent than its analogue MITO-CP. LA - English DB - MTMT ER - TY - CHAP AU - Bognár, Zita AU - Ramadan, Fadi AU - Bognár, Rita AU - Hocsák, Enikő AU - Andreidesz, Kitti AU - Gallyas, Ferenc ED - László, Buday ED - Miklós, Erdélyi ED - Beáta, Lontay ED - József, Mihály ED - Sinka, Rita ED - László, Virág ED - Gergely, Szakáts ED - Attila, Varga TI - Desethylamiodarone may have cytostatic potential on triple negative breast cancer T2 - Hungarian Molecular Life Sciences 2021 PB - Diamond Congress Kft. CY - Eger SN - 9786155270673 PY - 2021 SP - 181 EP - 181 PG - 1 UR - https://m2.mtmt.hu/api/publication/33095981 ID - 33095981 LA - English DB - MTMT ER - TY - CHAP AU - Ramadan, Fadi AU - Bognár, Rita AU - Szabó, Aliz AU - Gallyas, Ferenc AU - Bognár, Zita ED - László, Buday ED - Miklós, Erdélyi ED - Beáta, Lontay ED - József, Mihály ED - Sinka, Rita ED - László, Virág ED - Gergely, Szakáts ED - Attila, Varga TI - Involvement of mitochondrial mechanisms in the cytostatic effect of desethylamiodarone in B16F10 melanoma cells T2 - Hungarian Molecular Life Sciences 2021 PB - Diamond Congress Kft. CY - Eger SN - 9786155270673 PY - 2021 SP - 47 EP - 47 PG - 1 UR - https://m2.mtmt.hu/api/publication/33095958 ID - 33095958 LA - English DB - MTMT ER - TY - JOUR AU - Benyei, A. AU - Isbera, Mostafa AU - Bognár, Balázs AU - Gallyas, Ferenc AU - Jekő, József AU - Kalai, T. TI - Nitroxide with diphenylphosphino moiety: synthesis, supramolecular structure, biology and catalysis JF - ACTA CRYSTALLOGRAPHICA SECTION A: FOUNDATIONS AND ADVANCES J2 - ACTA CRYSTALLOGR A FOUND ADV VL - 77 PY - 2021 SP - C886 EP - C886 PG - 1 SN - 2053-2733 UR - https://m2.mtmt.hu/api/publication/32766022 ID - 32766022 N1 - Supplement: S LA - English DB - MTMT ER - TY - JOUR AU - Kovács, Dominika AU - Bagóné Vántus, Viola AU - Vámos, Eszter AU - Kálmán, Nikoletta AU - Schicho, Rudolf AU - Gallyas, Ferenc AU - Radnai, Balázs TI - Olaparib : A Clinically Applied PARP Inhibitor Protects from Experimental Crohn's Disease and Maintains Barrier Integrity by Improving Bioenergetics through Rescuing Glycolysis in Colonic Epithelial Cells JF - OXIDATIVE MEDICINE AND CELLULAR LONGEVITY J2 - OXID MED CELL LONGEV VL - 2021 PY - 2021 PG - 17 SN - 1942-0900 DO - 10.1155/2021/7308897 UR - https://m2.mtmt.hu/api/publication/32266554 ID - 32266554 AB - Crohn's disease (CD) is an inflammatory disorder of the intestines characterized by epithelial barrier dysfunction and mucosal damage. The activity of poly(ADP-ribose) polymerase-1 (PARP-1) is deeply involved in the pathomechanism of inflammation since it leads to energy depletion and mitochondrial failure in cells. Focusing on the epithelial barrier integrity and bioenergetics of epithelial cells, we investigated whether the clinically applied PARP inhibitor olaparib might improve experimental CD. We used the oral PARP inhibitor olaparib in the 2,4,6-trinitrobenzene sulfonic acid- (TNBS-) induced mouse colitis model. Inflammatory scoring, cytokine levels, colon histology, hematological analysis, and intestinal permeability were studied. Caco-2 monolayer culture was utilized as an epithelial barrier model, on which we used qPCR and light microscopy imaging, and measured impedance-based barrier integrity, FITC-dextran permeability, apoptosis, mitochondrial oxygen consumption rate, and extracellular acidification rate. Olaparib reduced the inflammation score, the concentration of IL-1β and IL-6, enhanced the level of IL-10, and decreased the intestinal permeability in TNBS-colitis. Blood cell ratios, such as lymphocyte to monocyte ratio, platelet to lymphocyte ratio, and neutrophil to lymphocyte ratio were improved. In H2O2-treated Caco-2 monolayer, olaparib decreased morphological changes, barrier permeability, and preserved barrier integrity. In oxidative stress, olaparib enhanced glycolysis (extracellular acidification rate), and it improved mitochondrial function (mitochondrial coupling efficiency, maximal respiration, and spare respiratory capacity) in epithelial cells. Olaparib, a PARP inhibitor used in human cancer therapy, improved experimental CD and protected intestinal barrier integrity by preventing its energetic collapse; therefore, it could be repurposed for the therapy of Crohn's disease. LA - English DB - MTMT ER -