TY - CHAP AU - Deák, Tamás AU - Kupi, Tünde AU - Szegedi, Ernő AU - Bisztray, György Dénes AU - Oláh, Róbert ED - M, Pinto TI - Identifying plant genes involved in Agrobacterium infection of grapevine T2 - IX International Symposium on Grapevine Physiology and Biotechnology PB - International Society for Horticultural Science (ISHS) CY - Leuven SN - 9789462611535 T3 - Acta Horticulturae, ISSN 0567-7572 ; 1157. PY - 2017 SP - 315 EP - 320 PG - 6 DO - 10.17660/ActaHortic.2017.1157.44 UR - https://m2.mtmt.hu/api/publication/3220098 ID - 3220098 AB - Crown gall caused by agrobacteria is one of the most serious grapevine diseases which still cannot be efficiently controlled. The pathogen genetically transforms host plant cells resulting in tumour growth. Transfer of a bacterial DNA segment (the T-DNA) from the bacterium to the host cell nucleus is mainly guided by bacterial virulence genes, while its integration into the host plant genome is determined by host genes. The ultimate goal of our work was to achieve Agrobacterium-resistance in grapevine by silencing host plant genes contributing to the transformation process. The candidate gene approach was applied to identify genes of interest (GOIs) in grapevine. The selection of GOIs with existing biological evidence for their involvement in the transformation process in model organisms was followed by an in- depth bioinformatic analysis of homologous gene families in grapevine. Selected grape gene families were the subject of gene expression analysis. Based on our results, grape homologues of the GTPase AtRab8, the Vir-interacting protein AtVIP1 and the histone protein AtH2A can be described as responsive to A. tumefaciens inoculation in grapevine. Hairpin-based RNAi silencing constructs selective for these genes have been established and their effect on crown gall tumour formation will be tested on transgenic grapevines. LA - English DB - MTMT ER - TY - JOUR AU - I, Forgács AU - B, Suller AU - Zok, Anikó AU - Pedryc, Andrzej AU - Oláh, Róbert AU - Deák, Tamás AU - Bisztray, György Dénes AU - Szegedi, Ernő TI - Establishment of grapevine embryogenic liquid culture and induced somatic embryogenesis JF - ACTA HORTICULTURAE: TECHNICAL COMMUNICATIONS OF ISHS J2 - ACTA HORTICULTURAE VL - 1157 PY - 2017 SP - 113 EP - 118 PG - 6 SN - 0567-7572 DO - 10.17660/ActaHortic.2017.1157.18 UR - https://m2.mtmt.hu/api/publication/3220097 ID - 3220097 N1 - Funding Agency and Grant Number: [TAMOP 4. 2. 1./B-09/01/KMR/2010-0005]; [OTKA 83121/2011] Funding text: We gratefully acknowledge the financial support from Grant TAMOP 4. 2. 1./B-09/01/KMR/2010-0005 and Grant OTKA 83121/2011. WoS:hiba:000431841200018 2020-08-30 08:50 befoglaló kiadók nem egyeznek AB - Somatic embryogenesis is a process in which somatic cells can be used to regenerate embryos and whole plants. Embryogenic cell suspensions of 'Chardonnay' and 'Richter 110' grapevine cultivars were established in 8 different liquid media, cultures had been later maintained in solid media. By the end of the sixth week, we achieved 7-fold increase of fresh weight in the case of the 'Chardonnay' cultivar, using MSM1 and CP2 liquid media. Four different pH values of hormone-free MSM1 media were tested in our experiments (pH 5.8, pH 4.6, pH 4.3 were adjusted before autoclaving; or pH 4.3 after autoclaving). In the case of pH 5.8, we measured a 16-fold increase of fresh weight of the 'Richter110' cultivar. Various culture densities (0.25-4 mg mL-1) were tested, and found that the one with 2 mg mL-1 density was the most efficient. Somatic embryogenesis proved to be significantly more efficient when the medium was supplemented with 0.5 g L-1 MES buffer [2-(N-morpholino) ethanesulfonic acid]. In this case, however, the embryos regenerated developed highly elongated hypocotyls, which inhibited the germination of the embryos as well as the successful regeneration of plants. In a half-strength MSM1 medium with a cell density of 1 mg mL-1, the cultures were composed of well synchronised globular stage embryos. In this setup, however, further differentiation was reversibly inhibited. This inhibition can be resolved by a second dilution of the cultures (to 1 mg mL-1 cell density). When hormone-free MSM1 medium was used after the second dilution, which was enriched with 1 g L-1 activated charcoal, synchronised and well developed cotyledonary stage embryos were differentiated in the liquid cultures by the sixth week. LA - English DB - MTMT ER - TY - JOUR AU - Inotai, Katalin AU - György, Zsuzsanna AU - Kindlovits, Sára AU - Várady, György AU - Zámboriné Németh, Éva TI - Evaluation of yarrow (Achillea) accessions by phytochemical and molecular genetic tools JF - JOURNAL OF APPLIED BOTANY AND FOOD QUALITY-ANGEWANDTE BOTANIK J2 - J APPL BOT FOOD QUAL VL - 89 PY - 2016 SP - 105 EP - 112 PG - 8 SN - 1613-9216 DO - 10.5073/JABFQ.2016.089.013 UR - https://m2.mtmt.hu/api/publication/3105151 ID - 3105151 LA - English DB - MTMT ER - TY - JOUR AU - Szalay, László AU - Ladányi, Márta AU - Hajnal, Veronika AU - Pedryc, Andrzej AU - Tóth, Magdolna TI - Changing of the flower bud frost hardiness in three Hungarian apricot cultivars JF - HORTICULTURAL SCIENCE J2 - HORTIC SCI VL - 43 PY - 2016 IS - 3 SP - 134 EP - 141 PG - 8 SN - 0862-867X DO - 10.17221/161/2015-HORTSCI UR - https://m2.mtmt.hu/api/publication/3105101 ID - 3105101 LA - English DB - MTMT ER - TY - JOUR AU - Andrey, S Marchev AU - Albena, T Dinkova-Kostova AU - György, Zsuzsanna AU - Mirmazloum, Iman AU - Ina, Y Aneva AU - Milen, I Georgiev TI - Rhodiola rosea L.: from golden root to green cell factories JF - PHYTOCHEMISTRY REVIEWS J2 - PHYTOCHEM REV VL - 15 PY - 2016 IS - 4 SP - 515 EP - 536 PG - 22 SN - 1568-7767 DO - 10.1007/s11101-016-9453-5 UR - https://m2.mtmt.hu/api/publication/3007802 ID - 3007802 LA - English DB - MTMT ER - TY - JOUR AU - LENDVAY, B AU - KADEREIT, J W AU - WESTBERG, E AU - CORNEJO, C AU - Pedryc, Andrzej AU - Höhn, Mária Margit TI - Phylogeography of Syringa josikaea (Oleaceae). Early Pleistocene divergence from East Asian relatives and survival in small populations in the Carpathians (Central Europe) TS - Early Pleistocene divergence from East Asian relatives and survival in small populations in the Carpathians (Central Europe) JF - BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY J2 - BIOL J LINN SOC VL - 119 PY - 2016 SP - 689 EP - 703 PG - 15 SN - 0024-4066 DO - 10.1111/bij.12499 UR - https://m2.mtmt.hu/api/publication/2808445 ID - 2808445 LA - English DB - MTMT ER - TY - CHAP AU - Deák, Tamás AU - Sz, Nagy László AU - Balogh, István AU - Bodor-Pesti, Péter AU - Bisztray, György Dénes ED - Veisz, Ottó Bálint ED - Polgár, Zsolt TI - Szőlő magvatlanság kimutatása SCAR-RFLP markerekkel T2 - XXII. Növénynemesítési Tudományos Nap: összefoglalók PB - Magyar Tudományos Akadémia (MTA) CY - Budapest SN - 9789633960851 PY - 2016 SP - 137 UR - https://m2.mtmt.hu/api/publication/2288231 ID - 2288231 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Sárdi, Éva AU - Tordai, E AU - Lukácsy, György AU - Stefanovitsné Bányai, Éva AU - Balogh, I ED - Veisz, Ottó Bálint ED - Polgár, Zsolt TI - A termőfajta és a fürtritkítás hatása a szőlőbogyók glükóz/fruktóz arányának alakulására Tokaj–Hegyalján T2 - XXII. Növénynemesítési Tudományos Nap: összefoglalók PB - Magyar Tudományos Akadémia (MTA) CY - Budapest SN - 9789633960851 PY - 2016 SP - 144 EP - 144 PG - 1 UR - https://m2.mtmt.hu/api/publication/1707964 ID - 1707964 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Sárdi, Éva AU - Blázovics, Anna AU - Tordai, E AU - Stefanovitsné Bányai, Éva ED - Veisz, Ottó Bálint ED - Polgár, Zsolt TI - Néhány zöldségfaj fogyasztásának hatása a lipid-anyagcserére és a koleszterin szintre állati kísérletekben T2 - XXII. Növénynemesítési Tudományos Nap: összefoglalók PB - Magyar Tudományos Akadémia (MTA) CY - Budapest SN - 9789633960851 PY - 2016 SP - 135 EP - 135 PG - 1 UR - https://m2.mtmt.hu/api/publication/1707960 ID - 1707960 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Pernesz, Gy AU - Oláh, Róbert AU - Tóth, V AU - Velich, I AU - Korbuly, J ED - Veisz, Ottó Bálint ED - Polgár, Zsolt TI - Új fagytűrő, vörös borszőlő fajták T2 - XXII. Növénynemesítési Tudományos Nap: összefoglalók PB - Magyar Tudományos Akadémia (MTA) CY - Budapest SN - 9789633960851 PY - 2016 SP - 140 EP - 140 PG - 1 UR - https://m2.mtmt.hu/api/publication/1315362 ID - 1315362 LA - Hungarian DB - MTMT ER -