TY - JOUR AU - Farkasinszky, Gergely AU - Péliné Szabó, Judit AU - Károlyi, Péter AU - Rácz, Szilvia AU - Dénes, Noémi AU - Papp, Tamás AU - Király, József AU - Szabó, Zsuzsanna AU - Kertész, István AU - Mező, Gábor AU - Halmos, Gábor AU - Képes, Zita AU - Trencsényi, György TI - In Vivo Imaging of Acute Hindlimb Ischaemia in Rat Model: A Pre-Clinical PET Study JF - PHARMACEUTICS J2 - PHARMACEUTICS VL - 16 PY - 2024 IS - 4 SP - 542 SN - 1999-4923 DO - 10.3390/pharmaceutics16040542 UR - https://m2.mtmt.hu/api/publication/34820368 ID - 34820368 AB - Background: To better understand ischaemia-related molecular alterations, temporal changes in angiogenic Aminopeptidase N (APN/CD13) expression and glucose metabolism were assessed with PET using a rat model of peripheral arterial disease (PAD). Methods: The mechanical occlusion of the base of the left hindlimb triggered using a tourniquet was applied to establish the ischaemia/reperfusion injury model in Fischer-344 rats. 2-[18F]FDG and [68Ga]Ga-NOTA-c(NGR) PET imaging performed 1, 3, 5, 7, and 10 days post-ischaemia induction was followed by Western blotting and immunohistochemical staining for APN/CD13 in ischaemic and control muscle tissue extracts. Results: Due to a cellular adaptation to hypoxia, a gradual increase in [68Ga]Ga-NOTA-c(NGR) and 2-[18F]FDG uptake was observed from post-intervention day 1 to 7 in the ischaemic hindlimbs, which was followed by a drop on day 10. Conforming pronounced angiogenic recovery, the NGR accretion of the ischaemic extremities differed significantly from the controls 5, 7, and 10 days after ischaemia induction (p ≤ 0.05), which correlated with the Western blot and immunohistochemical results. No remarkable radioactivity was depicted between the normally perfused hindlimbs of either the ischaemic or the control groups. Conclusions: The PET-based longitudinal assessment of angiogenesis-associated APN/CD13 expression and glucose metabolism during ischaemia may continue to broaden our knowledge on the pathophysiology of PAD. LA - English DB - MTMT ER - TY - JOUR AU - Bíró, Krisztina AU - Ujhelyi, Zoltán AU - Schlammadinger, Ágota AU - Rázsó, Katalin AU - Buchholcz, Gyula AU - Boda, Zoltán TI - Antitrombin-III-deficites gravidák - kezelési lehetőségek JF - GYÓGYSZERÉSZET J2 - GYÓGYSZERÉSZET VL - 68 PY - 2024 IS - 2 SP - 62 EP - 66 PG - 5 SN - 0017-6036 UR - https://m2.mtmt.hu/api/publication/34813506 ID - 34813506 LA - Hungarian DB - MTMT ER - TY - CONF AU - Fésüs, Adina AU - Matuz, M AU - Hambalek, H AU - Ruzsa, R AU - Tánczos, B AU - Bácskay, I AU - Illés, Á AU - Benkő, R TI - 4CPS-036 Evaluation of the diagnosis and antibiotic prescription pattern in patients hospitalised with urinary tract infections (UTIs) T2 - Section 4: Clinical pharmacy services PB - British Medical Journal Publishing Group PY - 2024 SP - A68.2 EP - A68 DO - 10.1136/ejhpharm-2024-eahp.140 UR - https://m2.mtmt.hu/api/publication/34756237 ID - 34756237 LA - English DB - MTMT ER - TY - CONF AU - Fésüs, Adina AU - Baluku, P AU - Sipos, É AU - Somodi, S AU - Vaskó, A AU - Lekli, I AU - Berczi-Kun, E AU - Bácskay, I TI - 4CPS-035 Impact of antibiotic stewardship programme (ASP) on antibiotic use and clinical outcomes in patients hospitalised with community-acquired pneumonia (CAP): retrospective observational before-after study T2 - Section 4: Clinical pharmacy services PB - British Medical Journal Publishing Group PY - 2024 SP - A68.1 EP - A68 DO - 10.1136/ejhpharm-2024-eahp.139 UR - https://m2.mtmt.hu/api/publication/34756232 ID - 34756232 LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Balázs István AU - Bahar, Bazeli AU - Annelies, Janssens AU - Lisztes, Erika AU - Racskó, Márk AU - Kelemen, Balázs AU - Herczeg, Mihály AU - Nagy, Tamás Milán AU - E Kövér, Katalin AU - Argha, Mitra AU - Attila, Borics AU - Bíró, Tamás AU - Thomas, Voets TI - Direct modulation of TRPM8 ion channels by rapamycin and analog macrolide immunosuppressants JF - ELIFE J2 - ELIFE PY - 2024 SN - 2050-084X UR - https://m2.mtmt.hu/api/publication/34722559 ID - 34722559 N1 - preprint DOI-ja: 10.1101/2024.02.01.578392 LA - English DB - MTMT ER - TY - JOUR AU - Ababneh, Haneen AU - Balogh, Enikő AU - Csiki, Dávid Máté AU - Lente, Gréta AU - Fenyvesi, Ferenc AU - Tóth, Andrea AU - Jeney, Viktória TI - High glucose promotes osteogenic differentiation of human lens epithelial cells through hypoxia-inducible factor (HIF) activation JF - JOURNAL OF CELLULAR PHYSIOLOGY J2 - J CELL PHYSIOL PY - 2024 PG - 12 SN - 0021-9541 DO - 10.1002/jcp.31211 UR - https://m2.mtmt.hu/api/publication/34618976 ID - 34618976 AB - Cataract, a leading cause of blindness, is characterised by lens opacification. Type 2 diabetes is associated with a two- to fivefold higher prevalence of cataracts. The risk of cataract formation increases with the duration of diabetes and the severity of hyperglycaemia. Hydroxyapatite deposition is present in cataractous lenses that could be the consequence of osteogenic differentiation and calcification of lens epithelial cells (LECs). We hypothesised that hyperglycaemia might promote the osteogenic differentiation of human LECs (HuLECs). Osteogenic medium (OM) containing excess phosphate and calcium with normal (1 g/L) or high (4.5 g/L) glucose was used to induce HuLEC calcification. High glucose accelerated and intensified OM-induced calcification of HuLECs, which was accompanied by hyperglycaemia-induced upregulation of the osteogenic markers Runx2, Sox9, alkaline phosphatase and osteocalcin, as well as nuclear translocation of Runx2. High glucose-induced calcification was abolished in Runx2-deficient HuLECs. Additionally, high glucose stabilised the regulatory alpha subunits of hypoxia-inducible factor 1 (HIF-1), triggered nuclear translocation of HIF-1 alpha and increased the expression of HIF-1 target genes. Gene silencing of HIF-1 alpha or HIF-2 alpha attenuated hyperglycaemia-induced calcification of HuLECs, while hypoxia mimetics (desferrioxamine, CoCl2) enhanced calcification of HuLECs under normal glucose conditions. Overall, this study suggests that high glucose promotes HuLEC calcification via Runx2 and the activation of the HIF-1 signalling pathway. These findings may provide new insights into the pathogenesis of diabetic cataracts, shedding light on potential factors for intervention to treat this sight-threatening condition. LA - English DB - MTMT ER - TY - JOUR AU - Tánczos, Bence AU - Vass, Virág AU - Szabó, Erzsébet AU - Lovas, Miklós AU - Kattoub, Rasha Ghanem AU - Bakai-Bereczki, Ilona AU - Borbás, Anikó AU - Herczegh, Pál AU - Tósaki, Árpád TI - Effects of H2S-donor ascorbic acid derivative and ischemia/reperfusion-induced injury in isolated rat hearts JF - EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES J2 - EUR J PHARM SCI VL - 195 PY - 2024 IS - - SP - 106721 SN - 0928-0987 DO - 10.1016/j.ejps.2024.106721 UR - https://m2.mtmt.hu/api/publication/34572686 ID - 34572686 LA - English DB - MTMT ER - TY - JOUR AU - Ratku, Balázs AU - Lőrincz, Hajnalka AU - Bak-Csiha, Sára AU - Sebestyén, Veronika AU - Berta, Eszter AU - Bodor, Miklós AU - Nagy, Endre AU - Szabó, Zoltán AU - Harangi, Mariann AU - Somodi, Sándor TI - Serum afamin and its implications in adult growth hormone deficiency: a prospective GH-withdrawal study JF - FRONTIERS IN ENDOCRINOLOGY J2 - FRONT ENDOCRINOL VL - 15 PY - 2024 SN - 1664-2392 DO - 10.3389/fendo.2024.1348046 UR - https://m2.mtmt.hu/api/publication/34564621 ID - 34564621 LA - English DB - MTMT ER - TY - JOUR AU - Skopkó, Boglárka Emese AU - Homoki, Judit Rita AU - Fazekas, Mónika Éva AU - Paholcsek, Melinda AU - Fauszt, Péter AU - Dávid, Péter AU - Stündl, László AU - Molnár, Piroska Bíróné AU - Forgács, Ildikó Noémi AU - Váradi, Judit AU - Bágyi, Kinga, Ágnes AU - Gálné Remenyik, Judit TI - Changes in the Composition of Unstimulated and Stimulated Saliva Due to Chewing Sour Cherry Gum and a Toothbrush Change JF - CELLS J2 - CELLS-BASEL VL - 13 PY - 2024 IS - 3 SN - 2073-4409 DO - 10.3390/cells13030251 UR - https://m2.mtmt.hu/api/publication/34546012 ID - 34546012 AB - Background: Our previous studies demonstrated that sour cherry anthocyanins (AC) reduce the salivary count of Streptococcus mutans and inhibit salivary amylase activity within 30 minutes after chewing AC gum. AC gum and changing toothbrushes after scaling reduced the Gram-negative species in the unstimulated salivary microbiota. The present study examined the effect of AC gums on salivary factors, including changes in microbiome. Methods: The study was conducted over three weeks with two groups; young adults (18–30) and adults (30–45). Ten participants changed their toothbrushes, while the other 10 participants did not change after the control period. After scaling, all participants received three doses of AC gum daily. The salivary mRNA and protein levels of cytokines, mucins, melatonin, and the microbiota of unstimulated and stimulated saliva were determined by polymerase chain reaction, enzyme-linked immunosorbent assay, and 16S rRNA gene sequencing. Results: Significantly higher levels of tumor necrosis factor α (TNFα), interleukin-1β (IL-1β), mucin5B (MUC5B), mucin7 (MUC7), and melatonin were detected in stimulated saliva. Correlation analysis of these factors with the microbiota showed positive correlations with the genera Lachnospiraceae, Eikenella, Saccharibacteria_(TM7), Streptococcus, Prevotella, and Haemophilus. Conclusions: AC chewing gum has a beneficial effect on the composition of the oral microbiome, and toothbrush replacement leads to changes in the levels of salivary pro-inflammatory cytokines. LA - English DB - MTMT ER - TY - JOUR AU - Feró, Orsolya AU - Varga, Dóra AU - Nagy, Éva AU - Karányi, Zsolt AU - Sipos, Éva AU - Engelhardt, József AU - Török, Nóra AU - Balogh, István AU - Vető, Borbála AU - Likó, István AU - Fóthi, Ábel AU - Szabó, Zoltán AU - Halmos, Gábor AU - Vécsei, László AU - Arányi, Tamás AU - Székvölgyi, Lóránt TI - DNA methylome, R-loop and clinical exome profiling of patients with sporadic amyotrophic lateral sclerosis JF - SCIENTIFIC DATA J2 - SCI DATA VL - 11 PY - 2024 IS - 1 PG - 12 SN - 2052-4463 DO - 10.1038/s41597-024-02985-y UR - https://m2.mtmt.hu/api/publication/34534306 ID - 34534306 N1 - MTA-DE Momentum, Genome Architecture and Recombination Research Group, Department of Molecular and Nanopharmaceutics, Faculty of Pharmacy, University of Debrecen, Debrecen, H-4032, Hungary Doctoral School of Pharmaceutical Sciences, Faculty of Pharmacy, University of Debrecen, Debrecen, H-4032, Hungary Department of Internal Medicine, Faculty of Medicine, University of Debrecen, Debrecen, H-4032, Hungary Department of Biopharmacy, Faculty of Pharmacy, University of Debrecen, Debrecen, H-4032, Hungary Department of Neurology, Albert Szent-Györgyi Medical School, University of Szeged, Szeged, Hungary Division of Clinical Genetics, Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, H-4032, Hungary Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary Department of Emergency Medicine, Faculty of Medicine, University of Debrecen, Debrecen, H-4032, Hungary Department of Molecular Biology, Semmelweis University, Budapest, Hungary Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, Debrecen, H-4032, Hungary Export Date: 13 February 2024; Cited By: 0; Correspondence Address: L. Székvölgyi; MTA-DE Momentum, Genome Architecture and Recombination Research Group, Department of Molecular and Nanopharmaceutics, Faculty of Pharmacy, University of Debrecen, Debrecen, H-4032, Hungary; email: lorantsz@med.unideb.hu; T. Arányi; Institute of Enzymology, Research Centre for Natural Sciences, Budapest, Hungary; email: aranyi.tamas@ttk.hu AB - Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the death of motor neurons, the aetiology of which is essentially unknown. Here, we present an integrative epigenomic study in blood samples from seven clinically characterised sporadic ALS patients to elucidate molecular factors associated with the disease. We used clinical exome sequencing (CES) to study DNA variants, DNA-RNA hybrid immunoprecipitation sequencing (DRIP-seq) to assess R-loop distribution, and reduced representation bisulfite sequencing (RRBS) to examine DNA methylation changes. The above datasets were combined to create a comprehensive repository of genetic and epigenetic changes associated with the ALS cases studied. This repository is well-suited to unveil new correlations within individual patients and across the entire patient cohort. The molecular attributes described here are expected to guide further mechanistic studies on ALS, shedding light on the underlying genetic causes and facilitating the development of new epigenetic therapies to combat this life-threatening disease. LA - English DB - MTMT ER -