TY - JOUR AU - Péntek, Adrienn AU - Pászty, Katalin AU - Apáti, Ágota TI - Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells JF - METHODS IN MOLECULAR BIOLOGY J2 - METHODS MOL BIOL VL - 1307 PY - 2016 SP - 141 EP - 147 PG - 7 SN - 1064-3745 DO - 10.1007/7651_2014_68 UR - https://m2.mtmt.hu/api/publication/2573910 ID - 2573910 AB - Measurement of changes in intracellular calcium concentration is one of the most common and useful tools for studying signal transduction pathways or cellular responses in basic research and drug screening purposes as well. Increasing number of such applications using human pluripotent stem cells and their derivatives requires development of calcium signal measurements for this special cell type. Here we describe a modified protocol for analysis of calcium signaling events in human embryonic stem cells, which can be used for other pluripotent cell types (such as iPSC) or their differentiated offspring as well. LA - English DB - MTMT ER - TY - CHAP AU - Szebényi, Kornélia AU - Péntek, Adrienn AU - Varga, Nóra AU - Erdei, Zsuzsa AU - Vőfély, Gergő AU - Orbán, Tamás I. AU - Sarkadi, Balázs AU - Apáti, Ágota ED - Sahu, Saura C ED - Casciano, Daniel A TI - Human Stem-Cell-Derived Cardiomyocytes in Drug Discovery and Toxicity Testing T2 - Handbook of Nanotoxicology, Nanomedicine and Stem Cell Use in Toxicology PB - John Wiley & Sons CY - London SN - 9781118856017 PY - 2014 SP - 267 EP - 298 PG - 32 DO - 10.1002/9781118856017.ch15 UR - https://m2.mtmt.hu/api/publication/2511378 ID - 2511378 N1 - Institute of Molecular Pharmacology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Membrane Research Group of the Hungarian Academy of Sciences, Semmelweis University, National Blood Service, Budapest, Hungary Chemical Technology Transfer Ltd., Budapest, Hungary Export Date: 25 January 2021 Correspondence Address: Szebényi, K.; Institute of Molecular Pharmacology, Hungary Összes idézések száma a WoS-ban: 0 Institute of Molecular Pharmacology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary Membrane Research Group of the Hungarian Academy of Sciences, Semmelweis University, National Blood Service, Budapest, Hungary Chemical Technology Transfer Ltd., Budapest, Hungary Export Date: 26 January 2021 Correspondence Address: Szebényi, K.; Institute of Molecular Pharmacology, Hungary AB - In this chapter, we review the methods of cardiomyocyte generation from human pluripotent and somatic stem cells, as well as from adult tissues through transdifferentiation. We describe the methods for stable genetic modification of human pluripotent stem cells, which can be applied to generate appropriate cardiomyocytes (CM) for drug screening, and introduce current methodologies for the preparation of cardiac progenitors for large-scale production. An overview of the current technologies for examining cardiotoxicity and the related key screening systems for drug development is provided. We describe selective reporter systems, which can be introduced into differentiated human CM and the potential application of human CM for disease models and personalized drug development. Finally, the future perspectives of these special systems in drug discovery are discussed. LA - English DB - MTMT ER - TY - BOOK AU - Nagyné Naszályi, Lívia AU - Ágnes, Szécsényi AU - Balázs, Debreczeni AU - Judith, Mihály AU - Szigyártó, Imola Csilla AU - András, Lőrincz AU - Attila, Bóta TI - Synthesis and characterization of zirconia-based drug nanocarriers PY - 2013 UR - https://m2.mtmt.hu/api/publication/30452708 ID - 30452708 AB - In modern drug research the role of formulation is increasing, since an appropriate matrix may ameliorate the absorption of a drug whose pharmacokinetic properties (solubility, stability, toxicity) are far from the optimum. Anti-cancer drugs are usually highly toxic and their solubility in aqueous phase is rather low. Our aim was to design and build up complex nanoparticles to realize targeted drug delivery into lung tumor via intravenous administration. Nanocarriers were composed of an inorganic silica/zirconia core coated with a polymeric shell that contained a polymeric matrix, complexing moieties, PEG chains, fluorescent dye and the drug. The different composition of the polymeric shell resulted in different dissolution kinetics in aqueous or biological media. Morphological analysis and stability conditions of the obtained nanocarriers were investigated using transmission electron microscopy (TEM), dynamic light scattering (DLS) and zeta potential measurements. Fourier-transform infrared spectroscopy (FTIR), mass spectrometry and thermogravimetry coupled to mass spectrometry (TG-MS) revealed the structure and composition of the polymeric shell. Degradation and release kinetics were studied in physiological salt solution. In vitro toxicity assay gave information about the biocompatibility of the complex nanocarriers. In vivo tests performed in tumor bearing SCID mice showed a long circulation time in the blood stream (several days) and the selective accumulation in lung tumor. LA - English DB - MTMT ER - TY - CHAP AU - Domonkos, Celesztina Diána AU - Zsila, F AU - Fitos, Ilona AU - Benéné Visy, Júlia ED - Magyar, Kémikusok Egyesülete TI - Evodiamin szérumfehérje kötődésének vizsgálata T2 - Vegyészkonferencia 2013 PB - Magyar Kémikusok Egyesülete (MKE) CY - Budapest SN - 9789639970366 PY - 2013 UR - https://m2.mtmt.hu/api/publication/2595161 ID - 2595161 LA - Hungarian DB - MTMT ER - TY - CHAP AU - Németh, K AU - Domonkos, Celesztina Diána AU - Szemán, Julianna AU - Mallareddy, J R AU - Sarnyai, V AU - Tóth, G AU - Jicsinszky, L AU - Szente, Lajos AU - Péter, Antal AU - Benéné Visy, Júlia ED - Kilár, Ferenc ED - Nagy, Laura ED - Kiss, Ibolya TI - Stereoselective analysis of amino acids and endomorphin analogue tetrapeptides by CE T2 - CECE 2013 10th International Interdisciplinary Meeting on Bioanalysis PB - University of Pécs CY - Pécs SN - 9789636425173 PY - 2013 SP - 80 EP - 80 PG - 1 UR - https://m2.mtmt.hu/api/publication/2594314 ID - 2594314 LA - English DB - MTMT ER - TY - CHAP AU - Gergely, András AU - Török, Tamás AU - Pászti, Zoltán AU - Bertóti, Imre AU - Mihály, Judith AU - Kálmán, Erika ED - Ajay, Kumar Mishra TI - Zinc-rich paint coatings containing either ionic surfactant-modified or functionalized multi-walled carbon nanotube-supported polypyrrole utilized to protect cold-rolled steel against corrosion T2 - Application of Carbon Nanotubes PB - Nova Science Publishers CY - New York, New York SN - 9781620819180 PY - 2013 SP - 211 EP - 258 PG - 48 UR - https://m2.mtmt.hu/api/publication/2563484 ID - 2563484 AB - The intense anodic action of sacrificial zinc pigments ensured viable galvanic function of the highly porous liquid zinc-rich paints (ZRPs) result in deteriorated longterm corrosion resistance often accompanied by cathodic delamination phenomena. In our approach, such a efficacy problem related to the corrosion preventive function of ZRPs is addressed by the application of intimately structured anodic inhibitor particles composed of nano-size alumina and either polyelectrolyte-modified or chemically functionalized multi-walled carbon nanotubes (MWCNT) supported polypyrrole (PPy) in one specific zinc-rich hybrid paint formulation providing balanced active-passive protective functionality. High dispersity of the nanotube-free PPy-deposited inhibitor particles (PDIPs) with uneven polymer distribution on the alumina carrier was confirmed by transmission electron microscopy (TEM) observations. Furthermore, the MWCNT-embedded PDIPs indicated almost complete surface coverage of the alumina-nanotube carriers by PPy with decreased microstructure dispersity which is attributed to the effect of double-flocculants type co-deposition of the oppositely charged polymers causing coalescence of the modified particles. Depending on the amount of the nanotubes and their proportion to the quantities of the deposited PPy and polyelectrolyte as well as the concentration of the surfactant, varied micron-scale association of the PDIPs in the suspensions of dissolved alkyd matrix was disclosed by rheology characterization carried out at particular solid contents similar to hybrid paint formulation. The evenly distributed but less densely packed nano-structure of PPy was evidenced on the polyelectrolyte-modified nanotubes by Fourier-transform infrared (FTIR) spectroscopy whereas more compact polymer film formation was confirmed on the surface of functionalized nanotubes. According to the greater electrical conductivity, enhanced electroactivity and reversibility of the nanotubeembedded PDIPs were indicated over the nanotube-free particles by cyclic voltammetry, depending on the type and the amount of the nanotubes and their modification. Protection function of the hybrid paint coatings (formulated with spherical zinc pigment at 70 wt.%) was investigated by immersion and salt-spray chamber tests over 254 and 142 day periods, respectively. Firm barrier nature of the nanotube-embedded PDIP contained hybrids was proved by electrochemical impedance spectroscopy (EIS) and radio-frequency glow-discharge optical-emission-spectroscopy (RF-GD-OES). Furthermore, due to the increased conductivity of the nanotube-embedded PDIPs cemented in epoxy primers optimally at 0.4 and 0.6 wt.%, altered corrosion preventive behaviour of the hybrid coatings was indicated by the positively polarized open-circuit potentials (OCPs) and the X-ray photoelectron spectroscopy (XPS) detected lower relative quantities of the interfacially accumulated zinc corrosion products, moderate oxidative degradation of the epoxy vehicle. Decreasing oxidative conversion of iron at the surface was indicated by XPS found to correlate with the increasing intensity of zinc corrosion and decreasing oxidative degradation of the epoxy binder, according to the higher nanotube contents of hybrid coatings. In addition, inhibited zinc corrosion caused low rate of oxidative degradation of epoxy, allowing increased durability of coating adhesion and cohesion thereby ensuring reliable protection by zinc-rich compositions. As a conclusion, modified or functionalized MWCNTs acting as unexchangeable doping agents promote enhanced reversibility and increased conductivity of PPy, forming nano-size inhibitor particles with advanced features. Thus, such inhibitor nano-particles in zinc-rich hybrid compositions afford improved barrier and high efficiency galvanic- cathodic corrosion preventive function, exceeding long-term protection capability of the conventional ZRPs. © 2013 Nova Science Publishers, Inc. All rights reserved. LA - English DB - MTMT ER - TY - CHAP AU - Wacha, András Ferenc AU - Onyestyák, György AU - Bóta, Attila ED - Cebrian, Pesci ED - Alejandra, Ciricillo TI - Structure and sorption dynamics of activated carbons from wooden precursors. Further possibilities to reveal anisotropic behaviors in the carbon skeleton TS - Further possibilities to reveal anisotropic behaviors in the carbon skeleton T2 - New Research on Carbon Materials PB - Nova Science Publishers CY - New York, New York SN - 9781624171871 PY - 2013 SP - 83 EP - 111 PG - 29 UR - https://m2.mtmt.hu/api/publication/2563115 ID - 2563115 AB - The anisotropic structure of activated carbons prepared from the trunks of different woods (Norwegian spruce, European beech, Pedunculate oak) was studied by small-angle X-ray scattering recorded by a two-dimensional detector. It was found that the type of the precursor has strong influence onto the structure of the end-product for low conversion rates. Higher (cca. 50 %) burn-off results in similar features of the micropore system. The decrease of the inherent anisotropy of the precursors during activation was also observed. Specific surface and pore size distribution were characterized by the well-established methods of static adsorption measurements (N2 adsorption, iodine number, methylene-blue adsorption). A novel, dynamic technique, such as frequency-response (FR) has also been applied taken advantage of its unique potentials. The obtained three-dimensional carbonized frames exhibit very low transport diffusional resistance due to the wide one-dimensional straight transport channels of micron size in tangential direction. The evolution of propane mass transport dynamics in the three series of the activated carbon preparations provides evidence for the complex diffusion processes in the pore system of the carbonized cell walls and for the significant differences in the mass transport dynamic occuring in micropores and the lowest range of mesopores depending on evolution of pore structure determined by the history of the activation. © 2013 by Nova Science Publishers, Inc. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Kékesi, Orsolya Sára AU - Nyitrai, Gabriella AU - Szabó, Pál Tamás AU - Fiáth, Richárd AU - Ulbert, István AU - Kardos, Julianna AU - Héja, László TI - GLIAL GABA TRANSPORTERS DOWNREGULATE ENHANCED NEURONAL ACTIVITY JF - GLIA J2 - GLIA VL - 61 PY - 2013 IS - 1 SP - S122 EP - S123 PG - 2 SN - 0894-1491 UR - https://m2.mtmt.hu/api/publication/2540335 ID - 2540335 N1 - 11th European Meeting on Glial Cell Function in Health and Disease JUL 03-06, 2013. Berlin, GERMANY SU 1 LA - English DB - MTMT ER - TY - JOUR AU - Schaper, L-A AU - Wei, X AU - Altmann, PJ AU - Öfele, K AU - Pöthig, A AU - Drees, M AU - Mink, János AU - Herdtweck, E AU - Bechlars, B AU - Herrmann, WA AU - Kühn, FE TI - Synthesis and comparison of transition metal complexes of abnormal and normal tetrazolylidenes: A neglected ligand species JF - INORGANIC CHEMISTRY J2 - INORG CHEM VL - 52 PY - 2013 IS - 12 SP - 7031 EP - 7044 PG - 14 SN - 0020-1669 DO - 10.1021/ic4005449 UR - https://m2.mtmt.hu/api/publication/2539087 ID - 2539087 N1 - Megjegyzés-23745395 : Wolfgang/A-3588-2010 Megjegyzés-23748905 : Wolfgang/A-3588-2010 AB - To date, only few examples of tetrazolylidene carbenes coordinated to transition metal complexes have been described. A direct comparison of "normal" tetrazolylidenes (1,4-substitution pattern) and "abnormal" tetrazolylidenes (also referred to as "mesoionic carbenes"; 1,3-substitution pattern) has not been performed at all. In this work, we describe coordination of both ligand types to a row of transition metals. For example, the first examples of tetrazolylidene Mo, Ag, or Ir complexes have been isolated and fully characterized. The mesoionic tetrazolylidene Ag compound 2d is shown to be a viable carbene transfer reagent. By means of NMR, IR, and Raman spectroscopy, as well as X-ray crystallography and computational results, the pronounced differences between the two ligand substitution patterns are highlighted. It is shown that simply changing the position of a methyl group from N3 to N4 in the heterocycle can cause enormous differences in ligand characteristics. © 2013 American Chemical Society. LA - English DB - MTMT ER - TY - JOUR AU - Haász, Veronika AU - Pöstényi, Zita AU - Polyák, András AU - Szigeti, A AU - Matula, Zsolt AU - Német, Katalin AU - Dabasi, Gabriella AU - Jóba, Róbert Péter AU - Jánoki, Győző AU - Jánoki, G AU - Balogh, Lajos TI - OSTEOGENIC CAPACITY OF CANINE ADIPOSE TISSUE-DERIVED MESENCHYMAL STEM CELLS FROM YOUNG VERSUS OLD DONORS: A COMPARATIVE STUDY JF - NUCLEAR MEDICINE REVIEW: CENTRAL AND EASTERN EUROPE J2 - NUCL MED REV CENT E EUR VL - 16 PY - 2013 IS - Suppl. A SP - A24 SN - 1506-9680 UR - https://m2.mtmt.hu/api/publication/2532216 ID - 2532216 N1 - # Besorolás ismeretlen #hozzárendelt szerző ismeretlen LA - English DB - MTMT ER -