TY - JOUR AU - Kemény, Kata Kira AU - Seres-Bokor, Adrienn AU - Barna, Tamara AU - Mirdamadi, Seyedmohsen AU - Gáspár, Róbert AU - Surányi, Andrea AU - Ducza, Eszter TI - Cooperation of aquaporin 5 and the adrenergic system in the initiation of birth in rat model JF - HELIYON J2 - HELIYON VL - 10 PY - 2024 IS - 17 PG - 11 SN - 2405-8440 DO - 10.1016/j.heliyon.2024.e37329 UR - https://m2.mtmt.hu/api/publication/35201852 ID - 35201852 N1 - ÚNKP-23-3 -SZTE-183 AB - Aquaporins (AQPs) are involved in the process of implantation, regulate myometrial contractions and cervical ripening, and maintain appropriate placental functioning. The molecular mechanism of these functions is not fully understood. Our study aimed to investigate the physiological significance of AQP5 during pregnancy and to determine the cooperation between the adrenergic system and the AQP5 in uterine contraction in the late-pregnant rat uterus. After administering AQP5 siRNA intraperitoneally to Sprague-Dawley rats, the length of the gestational period was determined and the changes in uterine contractions were measured in an isolated organ bath system. Pharmacological influence on AQP5 expression and uterine contraction was investigated by treatment with terbutaline (10 mg/kg, subcutaneously) and doxazosin (5 mg/kg, orally) in vivo; and mercuric chloride (HgCl2), in vitro. Moreover, the levels of cAMP response element binding protein (CREB) were measured in the uterus by an ELISA kit. The gestational period became shorter, AQP5 expression significantly decreased and rat uterus contraction increased after AQP5 siRNA treatment compared to the control. Treatment with terbutaline significantly increased AQP5 mRNA and protein expression after 30 minutes and continuously reduced it until 90 minutes, whereas doxazosin treatment did not significantly alter AQP5 expression. Treatment with the AQP5 antagonist HgCl2 increased spontaneous uterus contraction and decreased norepinephrine-induced uterus contraction with decreasing AQP5 expression in pregnant rat uterus. Moreover, the tocolytic effect through the adrenergic system was amplified in the presence of an AQP5 antagonist, presumably via the changes in cAMP level. In conclusion, our findings elucidate the collaborative role of aquaporin 5 (AQP5) and adrenergic systems in the regulation of uterine contractions in late-pregnant rats. Our findings suggest this may be a good starting point for developing a new tocolytic therapy. LA - English DB - MTMT ER - TY - JOUR AU - Pál, Z AU - Kádas, Zs AU - Kozinszky, Z AU - Surányi, Andrea TI - The Examination of Fetal Myocardium and Maternal and Fetal Features of Pregnancy Complicated By Gestational Diabetes Mellitus JF - Fortune Journal of Health Sciences J2 - Fortune J Health Sci VL - 7 PY - 2024 IS - 3 SP - 440 EP - 446 PG - 7 SN - 2644-2906 UR - https://m2.mtmt.hu/api/publication/35177894 ID - 35177894 N1 - https://www.fortunejournals.com/articles/the-examination-of-fetal-myocardium-and-maternal-and-fetal-features-of-pregnancy-complicated-by-gestational-diabetes-mellitus.html AB - Purpose: We examined the morphological factors affecting the growing foetus’ heart, the fetal and maternal relations of pregnancies complicated by diabetes mellitus. We seaked the severity of myocardium hyperplasia is in correlation with infant respiratory distress syndrome and cardiac failure. Our study was a prospective case- control study of pregnancies complicated by diabetes mellitus produce fetal myocardium hyperplasia. Methods: Two ultrasound examinations were carried out on 84 pregnant women, in accordance with the selection criteria (different types of diabetes mellitus and controll cases). The first ultrasound examination was carried out between the 24-28th weeks, the second between the 33-38th weeks of pregnancy. During the examinations we measured and averaged the fetal sizes, the estimated fetal weight, the quantity of amniotic fluid and the thickness of the ventricles and interventricular septum. Besides, we collected data from pregnant women in connection with the symptoms of diabetes mellitus, plus followed the outcome of these pregnancies. We compared these data against the our control group and a literature reference group. Results: Regarding the fetal myocardium and the interventricular septum, significantly higher values were registered compared to the control group, and 21% of the infants are macrosomic, 16% of them were born with intrauterine growth restriction. Conclusions: Based on the examinations we concluded that more complications occurred to foetuses and infants of obese pregnant women and those with diabetes mellitus than in the control groups. Thus, following the fetal myocardium’s parameters in the perinatal outcome of the mother’s glucose system’s discrepancies can be a well-applicable filtering method. LA - English DB - MTMT ER - TY - JOUR AU - Kemény, Kata Kira AU - Kozinszky, Zoltan AU - Altorjay, Ábel T. AU - Kolcsár, Bálint AU - Surányi, Andrea AU - Ducza, Eszter TI - Effect of Obesity on Aquaporin5 Expression in Human Placental and Uterus Tissues JF - JOURNAL OF CLINICAL MEDICINE J2 - J CLIN MED VL - 13 PY - 2024 IS - 15 SP - 4490 SN - 2077-0383 DO - 10.3390/jcm13154490 UR - https://m2.mtmt.hu/api/publication/35155320 ID - 35155320 AB - Background: Obesity and overweight are also becoming more prevalent among women of childbearing age and pregnant women. In maternal obesity, the activation of metabolic, inflammatory, and oxidative stress pathways is proven, which appears to be a key step in the pathological changes observed in placental and uterine function. Several recent studies have evidenced that aquaporins (AQPs) are critical players in adipose tissue biology and are involved in the onset of obesity. Methods: Our studies aimed to investigate the changes in placental volume and vascularization and measure the AQP5 expression and total antioxidant capacity (TAC) in the placenta and uterus tissues in obese and typical-weight mothers. We also aim to measure the AQP5 plasma concentration. Results: We found AQP5 dominance in the uterus and plasma at 34 weeks of normal pregnancy. The placental volume increased and the vascularization decreased in obese mothers compared to the control. The AQP5 expression increased in the uterus of the obese group and did not change in the placenta. The TAC decreased in the plasma of overweight mothers. Conclusion: We hypothesize that increased AQP5 expression prolongs the length of pregnancy and inhibits the onset of contractions. Based on our findings, we can develop diagnostic tests and provide new targets for tocolytic drug development. LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Richárd AU - Tóth, Zsófia AU - Keszthelyi, Lotti Lúcia AU - Török, Marianna AU - Ács, Nándor AU - Várbíró, Szabolcs AU - Keszthelyi, Márton AU - Lintner, Balázs TI - Management of Malignant Bowel Obstruction in Patients with Gynaecological Cancer. A Systematic Review TS - A Systematic Review JF - JOURNAL OF CLINICAL MEDICINE J2 - J CLIN MED VL - 13 PY - 2024 IS - 14 PG - 25 SN - 2077-0383 DO - 10.3390/jcm13144213 UR - https://m2.mtmt.hu/api/publication/35153557 ID - 35153557 N1 - Department of Obstetrics and Gynaecology, Semmelweis University, Budapest, 1082, Hungary Workgroup of Research Management, Doctoral School, Semmelweis University, Budapest, 1085, Hungary Department of Obstetrics and Gynaecology, University of Szeged, Szeged, 6725, Hungary Export Date: 31 August 2024 Correspondence Address: Keszthelyi, M.; Department of Obstetrics and Gynaecology, Hungary; email: keszthelyi.marton@semmelweis.hu AB - Objectives: This systematic review aimed to evaluate current surgical and non-surgical management strategies for malignant bowel obstruction (MBO) in patients with gynaecological cancer. Methods: Comprehensive literature searches were conducted across MEDLINE, Embase, CENTRAL, and Scopus, without restrictions on language or publication date. Following the removal of duplicates, 4866 articles were screened, with 34 meeting the inclusion criteria. Results: Surgical intervention remains the definitive treatment for MBO, offering longer symptom-free periods and improved survival, particularly when conservative methods fail. However, the selection of surgical candidates is crucial due to the high risk of morbidity and the potential for significant complications. Non-surgical treatments, such as the use of Gastrografin, Octreotide, and Dexamethasone, along with invasive procedures like nasogastric tubing, percutaneous gastrostomy, and stent placement, offer varying degrees of symptom relief and are often considered when surgery is not feasible. Conclusions: In this article we provide a potential therapeutic algorithm for the management of patients with MBO. This review underscores the urgent need for high-quality research to develop clear, evidence-based guidelines for MBO management in patients with gynaecologic cancer. Establishing standardised protocols will improve patient outcomes by aiding clinicians in making informed, individualised treatment decisions. LA - English DB - MTMT ER - TY - JOUR AU - Kata, Kira Kemény AU - Zoltan, Kozinszky AU - Ábel, T. Altorjay AU - Bálint, Kolcsár AU - Surányi, Andrea AU - Eszter, Ducza * TI - Effect of obesity on the aquaporin5 expression in the human placental and uterus tissues JF - PREPRINTS J2 - PREPRINTS PY - 2024 SN - 2310-287X UR - https://m2.mtmt.hu/api/publication/35135933 ID - 35135933 AB - Abstract: Obesity and overweight also affect women of childbearing age and pregnant women increasingly. In maternal obesity, the activation of metabolic, inflammatory, and oxidative stress pathways is proved, which appears to be a key step in placental and uterus function pathological changes. Several recent studies have evidenced that aquaporins (AQPs) are critical players in adipose tissue biology and are involved in the onset of obesity. Our studies aimed to investigate the changes in placental volume and vascularization and measure the AQP5 expression and total antioxidant capacity (TAC) in the placenta and uterus tissues in obese and normal-weight mothers. We also aim to measure the AQP5 plasma concentration. We found AQP5 dominance in the uterus and plasma at 34 weeks of normal pregnancy. The placental volume increased, and the vascularization decreased in obese mothers compared to the control. The AQP5 expression increased in the uterus of the obese group and did not change in the placenta. The TAC decreased in the plasma of overweight mothers. We hypothesize that increased AQP5 expression prolongs the length of pregnancy and inhibits the onset of contractions. Based on our findings, we can develop diagnostic tests and provide new targets for tocolytic drug development. LA - English DB - MTMT ER - TY - CONF AU - Kemény, Kata Kira AU - Surányi, Andrea AU - Altorjay, Ábel Tamás AU - Ducza, Eszter TI - The Relationship Between Obesity and Pregnancy – Molecular Biology Studies in Human Placental Samples T2 - Congressus Pharmaceuticus Hungaricus XVII. and EUFEPS Annual Meeting 2024 PB - Magyar Gyógyszerésztudományi Társaság (MGYT) C1 - Budapest PY - 2024 SP - 92 EP - 92 PG - 1 UR - https://m2.mtmt.hu/api/publication/34999655 ID - 34999655 LA - English DB - MTMT ER - TY - CONF AU - Szatmári, Péter AU - Kemény, Kata Kira AU - Surányi, Andrea AU - Altorjay, Ábel Tamás AU - Ducza, Eszter TI - Changes of P-glycoprotein expression in obese rat and human placentas T2 - Congressus Pharmaceuticus Hungaricus XVII. and EUFEPS Annual Meeting 2024 PB - Magyar Gyógyszerésztudományi Társaság (MGYT) C1 - Budapest PY - 2024 SP - 415 EP - 415 PG - 1 UR - https://m2.mtmt.hu/api/publication/34999597 ID - 34999597 LA - English DB - MTMT ER - TY - BOOK AU - Kemény, Kata Kira AU - Surányi, Andrea AU - Altorjay, Ábel Tamás AU - Ducza, Eszter TI - Investigation of obesity-related factors in human placental tissue samples PY - 2024 UR - https://m2.mtmt.hu/api/publication/34999568 ID - 34999568 LA - English DB - MTMT ER - TY - JOUR AU - Vincze, Márió Attila AU - Sikovanyecz, János Sebestyén AU - Földesi, Imre AU - Surányi, Andrea AU - Várbíró, Szabolcs AU - Németh, Gábor László AU - Sikovanyecz, János AU - Kozinszky, Zoltán TI - Galectin-13 and Laeverin Levels Interfere with Human Fetoplacental Growth JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 12 PG - 12 SN - 1661-6596 DO - 10.3390/ijms25126347 UR - https://m2.mtmt.hu/api/publication/34972138 ID - 34972138 N1 - Department of Obstetrics and Gynecology, University of Szeged, Szeged, H-6725, Hungary Department of Laboratory Medicine, University of Szeged, Szeged, H-6720, Hungary Capio Specialized Center for Gynecology, Stockholm, Solna, 182 88, Sweden Export Date: 21 August 2024 Correspondence Address: Kozinszky, Z.; Capio Specialized Center for Gynecology, Stockholm, Sweden; email: kozinszky@gmail.com Chemicals/CAS: aminopeptidase, 9031-94-1; metalloproteinase, 81669-70-7; Biomarkers; Galectins; LGALS13 protein, human; LVRN protein, human; Metalloproteases; Pregnancy Proteins Tradenames: Voluson 730 Expert, GE Healthcare, Austria Manufacturers: GE Healthcare, Austria AB - Galectin-13 (Gal-13) is predominantly produced by the syncytiotrophoblast, while laeverin is expressed on the outgrowing extravillous trophoblast, and both are thought to be biomarkers of preeclampsia. The aim of this study was to assess the correlation between concentrations of Gal-13 and laeverin measured in maternal serum and amniotic fluid at 16–22 weeks of gestation and the sonographic assessment of the fetoplacental measurements. Fetal biometric data and placental volume and perfusion indices were measured in 62 singleton pregnancies. Serum and amniotic levels of Gal-13 and laeverin levels were measured using a sandwich ELISA. Both amniotic fluid and serum Gal-13 levels expressed a negative correlation to the plasma laeverin level in mid-pregnancy. Serum laeverin level correlated positively with the gestational length at delivery (β = 0.39, p < 0.05), while the amniotic laeverin level correlated well with the abdominal circumference of the fetus (β = 0.44, p < 0.05). Furthermore, laeverin level in the amnion correlated positively with the estimated fetal weight (β = 0.48, p < 0.05) and with the placental volume (β = 0.32, p < 0.05). Logistic regression analyses revealed that a higher circulating Gal-13 level represents a slightly significant risk factor (OR: 1.01) for hypertension-related diseases during pregnancy. It is a novelty that laeverin can be detected in the amniotic fluid, and amnion laeverin concentration represents a potential biomarker of fetoplacental growth. LA - English DB - MTMT ER - TY - JOUR AU - Pásztor, Norbert AU - Veréb, Dániel AU - Király, István Előd AU - Kincses, Zsigmond Tamás AU - Palkó, András AU - Németh, Gábor László AU - Bajory, Zoltán AU - Fejes, Zsuzsanna TI - Testicular ultrasound measurement of testicular volume and epididymis diameters: Prediction of semen quality in a prospective study JF - IMAGING J2 - IMAGING VL - 16 PY - 2024 IS - 1 SP - 50 EP - 58 PG - 9 SN - 2732-0960 DO - 10.1556/1647.2024.00184 UR - https://m2.mtmt.hu/api/publication/34906053 ID - 34906053 N1 - Pásztor Norbert, Király István Előd, Németh Gábor szerzők affiliációjában tévesen lett feltüntetve: Department of Radiology, Albert Szent-Györgyi Health Centre, University of Szeged LA - English DB - MTMT ER -