TY - JOUR AU - Koderi Valappil, Sarshad AU - Shetty, Prateek AU - Deim, Zoltán AU - Terhes, Gabriella AU - Zsoldiné Urbán, Edit AU - Váczi, Sándor AU - Patai, Roland AU - Polgár, Tamás Ferenc AU - Pertics, Botond Zsombor AU - Schneider, György AU - Kovács, Tamás AU - Rákhely, Gábor TI - Survival Comes at a Cost: A Coevolution of Phage and Its Host Leads to Phage Resistance and Antibiotic Sensitivity of Pseudomonas aeruginosa Multidrug Resistant Strains JF - FRONTIERS IN MICROBIOLOGY J2 - FRONT MICROBIOL VL - 12 PY - 2021 PG - 17 SN - 1664-302X DO - 10.3389/fmicb.2021.783722 UR - https://m2.mtmt.hu/api/publication/32548543 ID - 32548543 AB - The increasing ineffectiveness of traditional antibiotics and the rise of multidrug resistant (MDR) bacteria have necessitated the revival of bacteriophage (phage) therapy. However, bacteria might also evolve resistance against phages. Phages and their bacterial hosts coexist in nature, resulting in a continuous coevolutionary competition for survival. We have isolated several clinical strains of Pseudomonas aeruginosa and phages that infect them. Among these, the PIAS (Phage Induced Antibiotic Sensitivity) phage belonging to the Myoviridae family can induce multistep genomic deletion in drug-resistant clinical strains of P. aeruginosa, producing a compromised drug efflux system in the bacterial host. We identified two types of mutant lines in the process: green mutants with SNPs (single nucleotide polymorphisms) and smaller deletions and brown mutants with large (∼250 kbp) genomic deletion. We demonstrated that PIAS used the MexXY-OprM system to initiate the infection. P. aeruginosa clogged PIAS phage infection by either modifying or deleting these receptors. The green mutant gaining phage resistance by SNPs could be overcome by evolved PIASs (E-PIASs) with a mutation in its tail-fiber protein. Characterization of the mutant phages will provide a deeper understanding of phage-host interaction. The coevolutionary process continued with large deletions in the same regions of the bacterial genomes to block the (E-)PIAS infection. These mutants gained phage resistance via either complete loss or substantial modifications of the phage receptor, MexXY-OprM, negating its essential role in antibiotic resistance. In vitro and in vivo studies indicated that combined use of PIAS and antibiotics could effectively inhibit P. aeruginosa growth. The phage can either eradicate bacteria or induce antibiotic sensitivity in MDR-resistant clinical strains. We have explored the potential use of combination therapy as an alternative approach against MDR P. aeruginosa infection. LA - English DB - MTMT ER - TY - THES AU - Pappné Ábrók, Marianna TI - Streptococcus agalactiae screening of pregnant women and detection of methicillin-resistant Staphylococcus aureus using MALDI-TOF mass spectrometry: application and improvement of the method [Várandós nők Streptococcus agalactiae szűrése és methicillin-rezisztens Staphylococcus aureus törzsek kimutatása MALDI-TOF tömegspektrometriával: alkalmazás és módszerfejlesztés] PB - Szegedi Tudományegyetem (SZTE) PY - 2021 SP - 67 DO - 10.14232/phd.10598 UR - https://m2.mtmt.hu/api/publication/32477085 ID - 32477085 LA - English DB - MTMT ER - TY - JOUR AU - Alexandra, Imre AU - Hanna, Viktória Rácz AU - Péter, Oláh AU - Zsuzsa, Antunovics AU - Csányiné Dóczi, Ilona AU - Kovács, Renátó AU - László, Majoros AU - István, Pócsi AU - Ksenija, Lopandic AU - Devin, Bendixsen AU - Rike, Stelkens AU - Walter, P. Pfiegler TI - Tuning Genomics for Highly Heterozygous and Polyploid Saccharomyces Genomes: Where did our Commercial and Clinical Yeasts come from? JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 68 PY - 2021 IS - Supplement 1 SP - 73 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/32471601 ID - 32471601 LA - English DB - MTMT ER - TY - JOUR AU - Imre, Alexandra AU - Kovács, Renátó AU - Pázmándi, Kitti AU - Jakab, Ágnes AU - V. Rácz, Hanna AU - Nemes, Dániel AU - Csányiné Dóczi, Ilona AU - Bácskay, Ildikó AU - Gácser, Attila AU - Farkas, Zoltán AU - Kovács, Károly AU - Majoros, László AU - Pócsi, István AU - P. Pflieger, Walter TI - Virulence Factors and In-Host Selection of Phenotypes in Infectious Probiotic Yeast Isolates JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 68 PY - 2021 IS - Supplement 1 SP - 73 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/32471568 ID - 32471568 LA - English DB - MTMT ER - TY - JOUR AU - Koderi Valappil, Sarshad AU - Deim, Zoltán AU - Terhes, Gabriella AU - Zsoldiné Urbán, Edit AU - Prateek, Shetty AU - Roland, Patai AU - György, Schneider AU - Tamás, Kovács AU - Rákhely, Gábor TI - Phage-Induced Antibiotic Sensitivity of Multidrug Resistant Pseudomonas Aeruginosa: A Perspective on Combined Phage-Antibiotic Therapy JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 68 PY - 2021 IS - Suppl. 1 SP - 47 EP - 47 PG - 1 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/32471534 ID - 32471534 LA - English DB - MTMT ER - TY - JOUR AU - Ulbert, Áron AU - Anett, Magyari AU - Hajdú, Edit AU - Zita, Túri AU - Burián, Katalin AU - Terhes, Gabriella TI - Epidemiological Characterization of HEV Infection, in Hungary JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 68 PY - 2021 IS - Suppl. 1 SP - 47 EP - 47 PG - 1 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/32471530 ID - 32471530 LA - English DB - MTMT ER - TY - JOUR AU - Sóki, József AU - Ulrike, Schumacher AU - Balázs, Horváth AU - Ágnes, Berényi AU - István, Nagy AU - Nagy, Erzsébet TI - Description of a Novel Carbapenemase From Bacteroides Xylanisolvens JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 68 PY - 2021 IS - Supplement 1 SP - 38 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/32471522 ID - 32471522 LA - English DB - MTMT ER - TY - JOUR AU - Baaity, Zain AU - Nagy, Erzsébet AU - Sóki, József TI - Phenotypic and Molecular Characterization of Carbapenem Heteroresistant Bacteroides Fragilis strains JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 68 PY - 2021 IS - Supplement 1 SP - 6 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/32471464 ID - 32471464 LA - English DB - MTMT ER - TY - JOUR AU - Baaity, Zain AU - Wafaa, Jamal AU - Vincent, O. Rotimi AU - Burián, Katalin AU - Nagy, Erzsébet AU - Somogyvári, Ferenc AU - Sóki, József TI - Metronidazole-resistant Bacteroidesstrains from Kuwait: A Molecular Study JF - ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA J2 - ACTA MICROBIOL IMMUNOL HUNG VL - 68 PY - 2021 IS - Supplement 1 SP - 5 SN - 1217-8950 UR - https://m2.mtmt.hu/api/publication/32471444 ID - 32471444 LA - English DB - MTMT ER - TY - CONF AU - Gajdács, Márió AU - Klaudia, Komáry AU - Burián, Katalin AU - Hajdú, Edit AU - Paulik, Edit AU - Szabó, Andrea TI - Development and pilot testing of a questionnaire to assess the knowledge-level and attitudes of junior doctors on infectious diseases and antimicrobial resistance T2 - Proceedings of the 1st International Electronic Conference on Antibiotics T3 - International Electronic Conference on Antibiotics PY - 2021 DO - 10.3390/ECA2021-09881 UR - https://m2.mtmt.hu/api/publication/32118834 ID - 32118834 LA - English DB - MTMT ER -