@article{MTMT:34794768, title = {Subarachnoid Hemorrhage Depletes Calcitonin Gene-Related Peptide Levels of Trigeminal Neurons in Rat Dura Mater}, url = {https://m2.mtmt.hu/api/publication/34794768}, author = {Masood, Thann Oon Saleh Saeed Saleh and Lakatos, Szandra and Karcsúné Kis, Gyöngyi and Ignácz, Melissza and Domoki, Ferenc and Rosta, Judit}, doi = {10.3390/cells13080653}, journal-iso = {CELLS-BASEL}, journal = {CELLS}, volume = {13}, unique-id = {34794768}, abstract = {Subarachnoid hemorrhage (SAH) remains a major cause of cerebrovascular morbidity, eliciting severe headaches and vasospasms that have been shown to inversely correlate with vasodilator calcitonin gene-related peptide (CGRP) levels. Although dura mater trigeminal afferents are an important source of intracranial CGRP, little is known about the effects of SAH on these neurons in preclinical models. The present study evaluated changes in CGRP levels and expression in trigeminal primary afferents innervating the dura mater 72 h after experimentally induced SAH in adult rats. SAH, eliciting marked damage revealed by neurological examination, significantly reduced the density of CGRP-immunoreactive nerve fibers both in the dura mater and the trigeminal caudal nucleus in the medulla but did not affect the total dural nerve fiber density. SAH attenuated ex vivo dural CGRP release by ~40% and in the trigeminal ganglion, reduced both CGRP mRNA levels and the number of highly CGRP-immunoreactive cell bodies. In summary, we provide novel complementary evidence that SAH negatively affects the integrity of the CGRP-expressing rat trigeminal neurons. Reduced CGRP levels suggest likely impaired meningeal neurovascular functions contributing to SAH complications. Further studies are to be performed to reveal the importance of impaired CGRP synthesis and its consequences in central sensory processing.}, year = {2024}, eissn = {2073-4409}, orcid-numbers = {Ignácz, Melissza/0009-0004-9154-0885; Domoki, Ferenc/0000-0002-5581-2167} } @article{MTMT:34783723, title = {Glycerol Trinitrate Acts Downstream of Calcitonin Gene-Related Peptide in Trigeminal Nociception—Evidence from Rodent Experiments with Anti-CGRP Antibody Fremanezumab}, url = {https://m2.mtmt.hu/api/publication/34783723}, author = {Benedicter, Nicola and Vogler, Birgit and Kuhn, Annette and Schramm, Jana and Mackenzie, Kimberly D. and Stratton, Jennifer and Dux, Mária and Messlinger, Karl}, doi = {10.3390/cells13070572}, journal-iso = {CELLS-BASEL}, journal = {CELLS}, volume = {13}, unique-id = {34783723}, abstract = {Calcitonin gene-related peptide (CGRP) and nitric oxide (NO) have been recognized as important mediators in migraine but their mechanisms of action and interaction have not been fully elucidated. Monoclonal anti-CGRP antibodies like fremanezumab are successful preventives of frequent migraine and can be used to study CGRP actions in preclinical experiments. Fremanezumab (30 mg/kg) or an isotype control monoclonal antibody was subcutaneously injected to Wistar rats of both sexes. One to several days later, glyceroltrinitrate (GTN, 5 mg/kg) mimicking nitric oxide (NO) was intraperitoneally injected, either once or for three consecutive days. The trigeminal ganglia were removed to determine the concentration of CGRP using an enzyme-linked immunosorbent assay (ELISA). In one series of experiments, the animals were trained to reach an attractive sugar solution, the access to which could be limited by mechanical or thermal barriers. Using a semi-automated registration system, the frequency of approaches to the source, the residence time at the source, and the consumed solution were registered. The results were compared with previous data of rats not treated with GTN. The CGRP concentration in the trigeminal ganglia was generally higher in male rats and tended to be increased in animals treated once with GTN, whereas the CGRP concentration decreased after repetitive GTN treatment. No significant difference in CGRP concentration was observed between animals having received fremanezumab or the control antibody. Animals treated with GTN generally spent less time at the source and consumed less sugar solution. Without barriers, there was no significant difference between animals having received fremanezumab or the control antibody. Under mechanical barrier conditions, all behavioral parameters tended to be reduced but animals that had received fremanezumab tended to be more active, partly compensating for the depressive effect of GTN. In conclusion, GTN treatment seems to increase the production of CGRP in the trigeminal ganglion independently of the antibodies applied, but repetitive GTN administration may deplete CGRP stores. GTN treatment generally tends to suppress the animals’ activity and increase facial sensitivity, which is partly compensated by fremanezumab through reduced CGRP signaling. If CGRP and NO signaling share the same pathway in sensitizing trigeminal afferents, GTN and NO may act downstream of CGRP to increase facial sensitivity.}, year = {2024}, eissn = {2073-4409}, orcid-numbers = {Dux, Mária/0000-0002-9941-8188; Messlinger, Karl/0000-0002-8099-3860} } @article{MTMT:34780663, title = {The effects of CO2 levels and body temperature on brain interstitial pH alterations during the induction of hypoxic-ischemic encephalopathy in newborn pigs}, url = {https://m2.mtmt.hu/api/publication/34780663}, author = {Remzső, Gábor and Kovács, Viktória and Tóth-Szűki, Valéria and Domoki, Ferenc}, doi = {10.1016/j.heliyon.2024.e28607}, journal-iso = {HELIYON}, journal = {HELIYON}, volume = {10}, unique-id = {34780663}, abstract = {Brain interstitial pH (pHbrain) alterations play a crucial role in the development of hypoxic-ischemic (HI) encephalopathy (HIE) caused by asphyxia in neonates. The newborn pig is one of the most suitable large animal models for studying HIE, however, compared to rats, experimental data on pHbrain alterations during HIE induction are limited. The major objective of the present study was thus to compare pHbrain changes during HIE development induced by experimental normocapnic hypoxia (H) or asphyxia (A), elicited with ventilation of a gas mixture containing 6%O2 or 6%O2/20%CO2, respectively for 20 min, under either normothermia (NT) or hypothermia (HT) (38.5 ± 0.5 °C or 33.5 ± 0.5 °C core temperature, respectively) in anesthetized piglets yielding four groups: H-NT, A-NT, H-HT, and A-HT. pHbrain changes during HI stress and the 60 min reoxygenation period were measured using a pH-selective microelectrode inserted into the parietal cortex through an open cranial window. In all groups, the pHbrain response to HI stress was acidosis, at the nadir pHbrain values dropped from the baseline of 7.27 ± 0.02 to H-NT:5.93 ± 0.30, A-NT:5.90 ± 0.52, H-HT:6.81 ± 0.27, and A-HT:6.27 ± 0.24 indicating that (1) H and A elicited similar, severe brain acidosis under NT greatly exceeding pH changes in arterial blood (pHa dropped to 7.24 ± 0.07 and 6.78 ± 0.03 from 7.52 ± 0.06 and 7.50 ± 0.05, respectively), and (2) HT ameliorated more the brain acidosis induced by H than by A. In all four groups, pHbrain was restored to baseline values without an alkalotic overshoot during the observed reoxygenation, Our findings suggest that under NT either H or A – both commonly employed HI stresses to elicit HIE in piglet models – would result in a similar acidotic pHbrain response without an alkalotic component either during the HI stress or the early reoxygenation period. © 2024 The Authors}, keywords = {hypothermia; Asphyxia; HYPOXIA}, year = {2024}, eissn = {2405-8440}, orcid-numbers = {Remzső, Gábor/0000-0002-3448-3835; Domoki, Ferenc/0000-0002-5581-2167} } @CONFERENCE{MTMT:34577105, title = {Exploring the gut-brain connection: How does microbiome composition relate to cognitive behavior in Wisket model rats?}, url = {https://m2.mtmt.hu/api/publication/34577105}, author = {Plesz, Szonja Bianka and Adlan, Leatitia Gabriella and Büki, Alexandra and Horváth, Gyöngyi and Ligeti, Balázs and Zádori, Zoltán Sándor and Kékesi, Gabriella}, booktitle = {7th Hungarian Neuroscience Doctoral Conference for Undergraduate Students, Graduate Students and Junior Post-Docs (HUNDOC) Booklet}, unique-id = {34577105}, year = {2024}, pages = {86}, orcid-numbers = {Büki, Alexandra/0000-0003-2078-5231; Horváth, Gyöngyi/0000-0002-6025-4577; Zádori, Zoltán Sándor/0000-0001-7312-618X; Kékesi, Gabriella/0000-0002-0185-2155} } @CONFERENCE{MTMT:34577022, title = {The Firmicutes/Bacteroidetes ratio in the triple-hit Wisket model rats of schizophrenia}, url = {https://m2.mtmt.hu/api/publication/34577022}, author = {Adlan, Leatitia Gabriella and Plesz, Szonja Bianka and Büki, Alexandra and Horváth, Gyöngyi and Ligeti, Balázs and Zádori, Zoltán Sándor and Kékesi, Gabriella}, booktitle = {International Neuroscience Conference, Pécs 2024}, unique-id = {34577022}, year = {2024}, pages = {74}, orcid-numbers = {Büki, Alexandra/0000-0003-2078-5231; Horváth, Gyöngyi/0000-0002-6025-4577; Zádori, Zoltán Sándor/0000-0001-7312-618X; Kékesi, Gabriella/0000-0002-0185-2155} } @CONFERENCE{MTMT:34576951, title = {Is gut dysbiosis associated with the motivational deficit observed in Wisket animals?}, url = {https://m2.mtmt.hu/api/publication/34576951}, author = {Plesz, Szonja Bianka and Adlan, Leatitia Gabriella and Büki, Alexandra and Ligeti, Balázs and Zádori, Zoltán Sándor and Kékesi, Gabriella}, booktitle = {International Neuroscience Conference, Pécs 2024}, unique-id = {34576951}, year = {2024}, pages = {73}, orcid-numbers = {Büki, Alexandra/0000-0003-2078-5231; Zádori, Zoltán Sándor/0000-0001-7312-618X; Kékesi, Gabriella/0000-0002-0185-2155} } @CONFERENCE{MTMT:34551935, title = {Altered multisensory integration in pediatric migraine without aura}, url = {https://m2.mtmt.hu/api/publication/34551935}, author = {Eördegh, Gabriella and Braunitzer, Gábor and Tót, Kálmán and Kiss, Ádám and Kóbor, Jenő Dezső and Nagy, Attila}, booktitle = {International Neuroscience Conference, Pécs 2024}, unique-id = {34551935}, abstract = {Alterations of visual processing in migraine are well known. It seems to be that multisensory information processing is altered in migraine, too, but this aspects of migraine are strongly underex- plored, especially regarding pediatric migraine. In the present study performances in visual and audiovisual associative equivalence learning of the pediatric patients with migraine without aura (aged 7-17.5 years) were compared to those of age- and sex-matched controls. The application of audiovisual stimuli significantly facilitated associative pair learning in healthy children and adolescents, but not in pediatric migraine patients. Although the performances were not significant worse in pediatric migraineurs compared those of the controls but the multisensory gain is significantly reduced in these patients. The results of this study suggest that multisensory integration is altered not only in adult mi- graineurs but this dysfunction can be already observed in pediatric migraine patients, too.}, year = {2024}, pages = {198-198}, orcid-numbers = {Eördegh, Gabriella/0000-0002-3707-3583; Braunitzer, Gábor/0000-0001-8983-5175; Tót, Kálmán/0000-0002-7641-7182; Kiss, Ádám/0000-0003-2597-7953} } @CONFERENCE{MTMT:34551903, title = {The effect of the applied visual stimuli with different level of complexity on audiovisual equivalence learning}, url = {https://m2.mtmt.hu/api/publication/34551903}, author = {Tót, Kálmán and Eördegh, Gabriella and Bátor, Brassó and Harcsa-Pintér, Noémi and Kiss, Ádám and Bodosi, Balázs and Kelemen, András and Nagy, Attila}, booktitle = {International Neuroscience Conference, Pécs 2024}, unique-id = {34551903}, abstract = {The Rutgers Acquired Equivalence Test (RAET) is a learning task, where the subjects learn visual stimuli pairs, and after the learning, they have to retrieve and generalize the previously learned as- sociations. We developed three audiovisual equivalence learning tests with the same structure, in which the antecedents were the same four different distinguishable sounds, but the consequents differed in complexity and semantic meanings. In the SoundFace test the consequents were four drawn faces, in the SoundFish four different colored fish (all same in size and shape), and in the SoundPolygon test blank geometric shapes. In the present study we compared the psychophysi- cal performances of 52 healthy volunteers between the three audiovisual tests. We asked whether there is any difference between the performances when visual stimuli differ in complexity and se- mantic meanings. In all parts (learning, retrieval and generalization) of the tests, the performance was significantly better in the SoundFace test compared to the other two, but there was no signifi- cant difference between the SoundFish and SoundPolygon. Our results suggest that the color infor- mation alone could not significantly enhance the effectiveness of the associative learning. However, the verbalizability of individual features of the visual stimuli seem to determine primarily the perfor- mances in associative equivalence learning.}, year = {2024}, pages = {196-196}, orcid-numbers = {Tót, Kálmán/0000-0002-7641-7182; Eördegh, Gabriella/0000-0002-3707-3583; Kiss, Ádám/0000-0003-2597-7953} } @CONFERENCE{MTMT:34551651, title = {Blink rate extraction from EEG recording}, url = {https://m2.mtmt.hu/api/publication/34551651}, author = {Kiss, Ádám and Kelemen, András and Nagy, Attila}, booktitle = {7th Hungarian Neuroscience Doctoral Conference for Undergraduate Students, Graduate Students and Junior Post-Docs (HUNDOC) Booklet}, unique-id = {34551651}, abstract = {EEG recordings include many components, such as EMG or blink components. In classical processing these are excluded, however, they may contain additional incomes. A possible side-channel information is the blink rate. A possible extraction of it is presented in this paper.}, year = {2024}, pages = {57-57}, orcid-numbers = {Kiss, Ádám/0000-0003-2597-7953} } @CONFERENCE{MTMT:34551634, title = {The effect of semantic meanings on audiovisual equivalence learning}, url = {https://m2.mtmt.hu/api/publication/34551634}, author = {Tót, Kálmán and Eördegh, Gabriella and Harcsa-Pintér, Noémi and Kiss, Ádám and Bátor, Brassó and Kelemen, András and Nagy, Attila}, booktitle = {7th Hungarian Neuroscience Doctoral Conference for Undergraduate Students, Graduate Students and Junior Post-Docs (HUNDOC) Booklet}, unique-id = {34551634}, abstract = {Equivalence learning is a type of associative learning, where the person learns that two stimuli are linked and they are equivalent with each other, if they share the same outcome, and this equivalence can be generalized to new instances. The Rutgers Acquired Equivalence Test (RAET) is a visual learning task to investigate this kind of learning. Based on the test we developed three audiovisual equivalence learning tests with the same structure. In all three tests the antecedents are the same four sound stimuli, but the consequents are visual stimuli with different complexity and semantic meanings. Our question is whether the semantic meanings affect the performances in the learning tests. The results of our research with healthy volunteers showed that if more semantic label corresponds to the applied visual stimuli, it leads to better performances in the learning test.}, year = {2024}, pages = {110-110}, orcid-numbers = {Tót, Kálmán/0000-0002-7641-7182; Eördegh, Gabriella/0000-0002-3707-3583; Kiss, Ádám/0000-0003-2597-7953} }