TY - JOUR AU - Sándor, Noémi AU - Schneider, Andrea E. AU - Matola, Alexandra Tünde AU - Barbai, Veronika H. AU - Bencze, Dániel AU - Hammad, Hani Hashim AU - Papp, Alexandra Éva AU - Kövesdi, Dorottya AU - Uzonyi, Barbara AU - Józsi, Mihály TI - The human factor H protein family – an update JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 15 PY - 2024 SN - 1664-3224 DO - 10.3389/fimmu.2024.1135490 UR - https://m2.mtmt.hu/api/publication/34706689 ID - 34706689 N1 - Department of Immunology, ELTE Eötvös Loránd University, Budapest, Hungary HUN-REN-ELTE Complement Research Group, Hungarian Research Network, Budapest, Hungary Export Date: 14 March 2024 Correspondence Address: Józsi, M.; Department of Immunology, Hungary; email: mihaly.jozsi@ttk.elte.hu AB - Complement is an ancient and complex network of the immune system and, as such, it plays vital physiological roles, but it is also involved in numerous pathological processes. The proper regulation of the complement system is important to allow its sufficient and targeted activity without deleterious side-effects. Factor H is a major complement regulator, and together with its splice variant factor H-like protein 1 and the five human factor H-related (FHR) proteins, they have been linked to various diseases. The role of factor H in inhibiting complement activation is well studied, but the function of the FHRs is less characterized. Current evidence supports the main role of the FHRs as enhancers of complement activation and opsonization, i.e., counter-balancing the inhibitory effect of factor H. FHRs emerge as soluble pattern recognition molecules and positive regulators of the complement system. In addition, factor H and some of the FHR proteins were shown to modulate the activity of immune cells, a non-canonical function outside the complement cascade. Recent efforts have intensified to study factor H and the FHRs and develop new tools for the distinction, quantification and functional characterization of members of this protein family. Here, we provide an update and overview on the versatile roles of factor H family proteins, what we know about their biological functions in healthy conditions and in diseases. LA - English DB - MTMT ER - TY - JOUR AU - Szöllősi, Dávid AU - Hajdrik, Polett AU - Tordai, Hedvig AU - Horváth, Ildikó AU - Veres, Dániel AU - Gillich, Bernadett AU - Shailaja, Kanni Das AU - Smeller, László AU - Bergmann, Ralf Konrad AU - Bachmann, Michael AU - Mihály, Judith AU - Gaál, Anikó AU - Jezsó, Bálint AU - Barátki, Balázs Lajos AU - Kövesdi, Dorottya AU - Bősze, Szilvia AU - Szabó, Ildikó AU - Felföldi, Tamás AU - Oszwald, Erzsébet AU - Padmanabhan, Parasuraman AU - Gulyás, Balázs Zoltán AU - Hamdani, Nazha AU - Máthé, Domokos AU - Varga, Zoltán AU - Szigeti, Krisztián TI - Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 14 SN - 2045-2322 DO - 10.1038/s41598-023-45628-9 UR - https://m2.mtmt.hu/api/publication/34267696 ID - 34267696 N1 - Department of Biophysics and Radiation Biology, Semmelweis University, 37-47 Tűzoltó Street, Budapest, 1094, Hungary Institute for Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf, 400 Bautzner Landstraße, Dresden, 01328, Germany Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, 2 Magyar Tudósok Körútja, Budapest, 1117, Hungary Doctoral School of Biology and Institute of Biology, Eötvös Loránd University, 1/C Pázmány Péter Sétány, Budapest, 1117, Hungary Department of Immunology, ELTE Eötvös Loránd University, 1/C Pázmány Péter Sétány, Budapest, 1117, Hungary MTA-ELTE Complement Research Group, Eötvös Loránd Research Network (ELKH), 1/A Pázmány Péter Sétány, Budapest, 1117, Hungary ELKH-ELTE Research Group of Peptide Chemistry, Eötvös L. Research Network, Eötvös L. University, 1/A Pázmány Péter Sétány, Budapest, 1117, Hungary Department of Microbiology, ELTE Eötvös Loránd University, 1/C Pázmány Péter Sétány, Budapest, 1117, Hungary Centre for Ecological Research, Institute of Aquatic Ecology, 29 Karolina Road, Budapest, 1113, Hungary Department of Anatomy, Histology, and Embryology, Semmelweis University, 58 Tűzoltó Street, Budapest, 1094, Hungary Lee Kong Chian School of Medicine, Nanyang Technological University, 11 Mandalay Road, Singapore, 30823, Singapore Cognitive Neuroimaging Centre, Nanyang Technological University, 59 Nanyang Drive, Singapore, 636921, Singapore Department of Cellular and Translational Physiology, Institute of Physiology, Ruhr University Bochum, Bochum, 44801, Germany HCEMM-Cardiovascular Research Group, Department of Pharmacology and Pharmacotherapy, University of Budapest, Budapest, 1089, Hungary CROmed Translational Research Centers, 37-47 Tűzoltó Street, Budapest, 1094, Hungary In Vivo Imaging Advanced Core Facility, Hungarian Center of Excellence for Molecular Medicine (HCEMM), 37-47 Tűzoltó Street, Budapest, 1094, Hungary Export Date: 30 January 2024 Correspondence Address: Szigeti, K.; Department of Biophysics and Radiation Biology, 37-47 Tűzoltó Street, Hungary; email: krisztian.szigeti@gmail.com Funding details: Horizon 2020 Framework Programme, H2020, 739593 Funding details: European Commission, EC, 859890 Funding details: Magyar Tudományos Akadémia, MTA Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI, 2020.1.16-Jövő-2021–00013, 2020–1.1.2-PIACI-KFI-2020–00021, TKP2021-EGA-31 Funding details: Nemzeti Kutatási, Fejlesztési és Innovaciós Alap, NKFIA, EFOP-1.8.0-VEKOP-17-2017-00001 Funding details: Innovációs és Technológiai Minisztérium Funding text 1: The authors thank Miklós Geiszt for his contributions to the in vivo experiments, Mihály Kálmán and Ferenc Kilin for their help with the TEM measurements, Wouter Jong and Bart van den Berg van Saparoea for the pHbpD(Δd1) plasmid. Funding text 2: Open access funding provided by Semmelweis University. Zoltán Varga was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences, the ÚNKP-21-5 Bolyai + New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research, Development and Innovation Fund. Ildikó Szabó and Szilvia Bősze thank for the support of grant EFOP-1.8.0-VEKOP-17-2017-00001 and for the ELTE Thematic Excellence Programme the 2018-1.2.1-NKP-2018-00005 project (under the 2018-1.2.1-NKP funding scheme) provided by the Hungarian Ministry for Innovation and Technology, Hungary. Kanni Das Shailaja received support from the European Union under project H2020-SmartAge grant Nr. 859890. This work was supported by The European Union’s Horizon 2020 Research And Innovation Program, grant agreement No 739593: HCEMM, supported by EU Programme: H2020-EU.4.a. This work was also partly funded by grants from the Hungarian National Research, Development and Innovation Office (Thematic Excellence Program, TKP-BIOImaging, financed under the 2020–4.1.1-TKP2020 funding scheme, Investment to the Future 2020.1.16-Jövő-2021–00013, TKP2021-EGA-31 and 2020–1.1.2-PIACI-KFI-2020–00021). AB - The important roles of bacterial outer membrane vesicles (OMVs) in various diseases and their emergence as a promising platform for vaccine development and targeted drug delivery necessitates the development of imaging techniques suitable for quantifying their biodistribution with high precision. To address this requirement, we aimed to develop an OMV specific radiolabeling technique for positron emission tomography (PET). A novel bacterial strain ( E. coli BL21(DE3) ΔnlpI, ΔlpxM ) was created for efficient OMV production, and OMVs were characterized using various methods. SpyCatcher was anchored to the OMV outer membrane using autotransporter-based surface display systems. Synthetic SpyTag-NODAGA conjugates were tested for OMV surface binding and 64 Cu labeling efficiency. The final labeling protocol shows a radiochemical purity of 100% with a ~ 29% radiolabeling efficiency and excellent serum stability. The in vivo biodistribution of OMVs labeled with 64 Cu was determined in mice using PET/MRI imaging which revealed that the biodistribution of radiolabeled OMVs in mice is characteristic of previously reported data with the highest organ uptakes corresponding to the liver and spleen 3, 6, and 12 h following intravenous administration. This novel method can serve as a basis for a general OMV radiolabeling scheme and could be used in vaccine- and drug-carrier development based on bioengineered OMVs. LA - English DB - MTMT ER - TY - JOUR AU - Józsi, Mihály TI - Factor H family proteins as modulators of complement activation for better or worse JF - EUROPEAN JOURNAL OF IMMUNOLOGY J2 - EUR J IMMUNOL VL - 53 PY - 2023 SP - 38 EP - 38 PG - 1 SN - 0014-2980 UR - https://m2.mtmt.hu/api/publication/34213739 ID - 34213739 LA - English DB - MTMT ER - TY - JOUR AU - Szeles, Zsolt AU - Petheő, Gábor L. AU - Szikora, Bence AU - Kacskovics, Imre AU - Geiszt, Miklós TI - A novel monoclonal antibody reveals the enrichment of NADPH oxidase 5 in human splenic endothelial cells JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 13 PY - 2023 IS - 1 PG - 13 SN - 2045-2322 DO - 10.1038/s41598-023-44018-5 UR - https://m2.mtmt.hu/api/publication/34198665 ID - 34198665 AB - Members of the NOX/DUOX family of NADPH oxidases are responsible for regulated ROS production in diverse cells and tissues. Detection of NOX/DUOX proteins at the protein level remains an important challenge in the field. Here we report the development and characterization of a novel anti-NOX5 monoclonal antibody, which recognizes the human NOX5 protein in both Western blot, immunocytochemistry, and histochemistry applications. With the help of the antibody we could successfully detect both heterologously and endogenously expressed NOX5 in mammalian cells. Furthermore, we could also detect NOX5 protein in the human spleen, testis, and ovary. Immunohistochemical studies on human testis revealed that NOX5 localized to spermatogenic cells. This expression pattern was also supported by the result of in silico analysis of single-cell RNA sequencing data that indicated that NOX5 protein is present in developing spermatids and spermatocytes. Mature spermatozoa, however, did not contain detectable NOX5. In the human ovary, both immunostaining and single-cell RNA sequencing suggest that NOX5 is expressed in interstitial fibroblasts and theca cells. We also analyzed vascular cells for the presence of NOX5 and we found that NOX5 expression is a fairly specific feature of splenic endothelial cells. LA - English DB - MTMT ER - TY - JOUR AU - Homonnayné Barbai, Veronika AU - Papp, Alexandra Éva AU - Bencze, Dániel AU - Uzonyi, Barbara AU - Józsi, Mihály TI - 230 Interaction of factor H and factor H-related proteins with S and N proteins of SARS-CoV-2 JF - IMMUNOBIOLOGY J2 - IMMUNOBIOLOGY VL - 228 PY - 2023 IS - 5 SN - 0171-2985 DO - 10.1016/j.imbio.2023.152680 UR - https://m2.mtmt.hu/api/publication/34133958 ID - 34133958 LA - English DB - MTMT ER - TY - JOUR AU - Papp, Alexandra Éva AU - Bencze, Dániel AU - Uzonyi, Barbara AU - Márquez-Tirado, Bárbara AU - de Jorge, Elena Goicoechea AU - Józsi, Mihály TI - 177 Factor H-related proteins bind to extracellular matrix components and affect complement activation JF - IMMUNOBIOLOGY J2 - IMMUNOBIOLOGY VL - 228 PY - 2023 IS - 5 SN - 0171-2985 DO - 10.1016/j.imbio.2023.152627 UR - https://m2.mtmt.hu/api/publication/34133943 ID - 34133943 LA - English DB - MTMT ER - TY - JOUR AU - Lazar, Jozsef AU - Antal-Szalmás, Péter AU - Kurucz, Istvan AU - Ferenczi, Annamaria AU - Józsi, Mihály AU - Tornyi, Ilona AU - Muller, Monika AU - Fekete, János Tibor AU - Lamont, John AU - FitzGerald, Peter AU - Gall-Debreceni, Anna AU - Kádas, János AU - Vida, András AU - Tardieu, Nadege AU - Kieffer, Yann AU - Jullien, Anne AU - Guergova-Kuras, Mariana AU - Hempel, William AU - Kovacs, Andras AU - Kardos, Tamas AU - Bittner, Nóra AU - Csanky, Eszter AU - Szilasi, Mária AU - Losonczy, György AU - Szondy, Klára AU - Gálffy, Gabriella AU - Csada, Edit AU - Szalontai, Klára Margit AU - Somfay, Attila AU - Malka, David AU - Cottu, Paul AU - Bogos, Krisztina AU - Takacs, Laszlo TI - Large-Scale Plasma Proteome Epitome Profiling is an Efficient Tool for the Discovery of Cancer Biomarkers JF - MOLECULAR & CELLULAR PROTEOMICS J2 - MOL CELL PROTEOMICS VL - 22 PY - 2023 IS - 7 PG - 20 SN - 1535-9476 DO - 10.1016/j.mcpro.2023.100580 UR - https://m2.mtmt.hu/api/publication/33999296 ID - 33999296 N1 - Biosystems International Kft., Debrecen, Hungary Biosystems Immunolab Zrt., Debrecen, Hungary Department of Laboratory Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Department of Immunology, ELTE Eötvös Loránd University, Budapest, Hungary MTA-ELTE Complement Research Group, Eötvös Loránd Research Network (ELKH), Budapest, Hungary Department of Human Genetics, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Adware Research Kft., Balatonfüred, Hungary Department of Bioinformatics, Semmelweis University, Budapest, Hungary Randox Laboratories Ltd, Crumlin, United Kingdom Bio-systems International SAS, Evry, France Department of Pulmonology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Department of Pulmonology, Miskolc Semmelweis Hospital, University Hospital, Miskolc, Hungary Department of Pulmonology, Faculty of Medicine, Semmelweis University, Budapest, Hungary Csongrád County Hospital of Chest Diseases, Deszk, Hungary Department of Pulmonology, Faculty of Medicine, University of Szeged, Deszk, Hungary Department of Medical Oncology, Gustave Roussy, Villejuif, France Department of Medical Oncology, Institut Curie, Paris, France National Koranyi Institute for Pulmonology, Budapest, Hungary Cited By :2 Export Date: 14 March 2024 CODEN: MCPOB Correspondence Address: Lazar, J.; Biosystems International Kft.Hungary; email: jozsef.lazar@biosys-ilab.com Correspondence Address: Takacs, L.; Biosystems International Kft.Hungary; email: laszlo.takacs@biosys-ilab.com LA - English DB - MTMT ER - TY - JOUR AU - Marx, Anita AU - Osváth, Magdolna AU - Szikora, Bence AU - Pipek, Orsolya Anna AU - Csabai, István AU - Nagy, Ákos AU - Bödör, Csaba AU - Matula, Zsolt AU - Nagy, Ginette AU - Bors, András AU - Uher, Ferenc AU - Mikala, Gábor AU - Vályi-Nagy, István AU - Kacskovics, Imre TI - Liquid biopsy-based monitoring of residual disease in multiple myeloma by analysis of the rearranged immunoglobulin genes–A feasibility study JF - PLOS ONE J2 - PLOS ONE VL - 18 PY - 2023 IS - 5 PG - 23 SN - 1932-6203 DO - 10.1371/journal.pone.0285696 UR - https://m2.mtmt.hu/api/publication/33893006 ID - 33893006 N1 - * Megosztott szerzőség AB - The need for sensitive monitoring of minimal/measurable residual disease (MRD) in multiple myeloma emerged as novel therapies led to deeper responses. Moreover, the potential benefits of blood-based analyses, the so-called liquid biopsy is prompting more and more studies to assess its feasibility. Considering these recent demands, we aimed to optimize a highly sensitive molecular system based on the rearranged immunoglobulin (Ig) genes to monitor MRD from peripheral blood. We analyzed a small group of myeloma patients with the high-risk t(4;14) translocation, using next-generation sequencing of Ig genes and droplet digital PCR of patient-specific Ig heavy chain (IgH) sequences. Moreover, well established monitoring methods such as multiparametric flow cytometry and RT-qPCR of the fusion transcript IgH :: MMSET (IgH and multiple myeloma SET domain-containing protein) were utilized to evaluate the feasibility of these novel molecular tools. Serum measurements of M-protein and free light chains together with the clinical assessment by the treating physician served as routine clinical data. We found significant correlation between our molecular data and clinical parameters, using Spearman correlations. While the comparisons of the Ig-based methods and the other monitoring methods (flow cytometry, qPCR) were not statistically evaluable, we found common trends in their target detection. Regarding longitudinal disease monitoring, the applied methods yielded complementary information thus increasing the reliability of MRD evaluation. We also detected indications of early relapse before clinical signs, although this implication needs further verification in a larger patient cohort. LA - English DB - MTMT ER - TY - JOUR AU - Bulla, Roberta AU - Józsi, Mihály TI - Editorial: Updates on the complement system in kidney diseases JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 14 PY - 2023 SN - 1664-3224 DO - 10.3389/fimmu.2023.1196487 UR - https://m2.mtmt.hu/api/publication/33786674 ID - 33786674 LA - English DB - MTMT ER - TY - JOUR AU - Mussa, Ali AU - Afolabi, Hafeez Abiola AU - Syed, Nazmul Huda AU - Talib, Mustafa Mohammed Hamid AU - Murtadha, Ahmad Hafiz AU - Hajissa, Khalid AU - Mokhtar, Noor Fatmawati AU - Mohamud, Rohimah AU - Hassan, Rosline TI - The NF-κB Transcriptional Network Is a High-Dose Vitamin C-Targetable Vulnerability in Breast Cancer JF - BIOMEDICINES J2 - BIOMEDICINES VL - 11 PY - 2023 IS - 4 SN - 2227-9059 DO - 10.3390/biomedicines11041060 UR - https://m2.mtmt.hu/api/publication/33726885 ID - 33726885 AB - Breast cancer (BC) is the most common cancer type among women with a distinct clinical presentation, but the survival rate remains moderate despite advances in multimodal therapy. Consequently, a deeper understanding of the molecular etiology is required for the development of more effective treatments for BC. The relationship between inflammation and tumorigenesis is well established, and the activation of the pro-inflammatory transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is frequently identified in BC. Constitutive NF-κB activation is linked to cell survival, metastasis, proliferation, and hormonal, chemo-, and radiotherapy resistance. Moreover, the crosstalk between NF-κB and other transcription factors is well documented. It is reported that vitamin C plays a key role in preventing and treating a number of pathological conditions, including cancer, when administered at remarkably high doses. Indeed, vitamin C can regulate the activation of NF-κB by inhibiting specific NF-κB-dependent genes and multiple stimuli. In this review, we examine the various NF-κB impacts on BC development. We also provide some insight into how the NF-κB network may be targeted as a potential vulnerability by using natural pro-oxidant therapies such as vitamin C. LA - English DB - MTMT ER -