@article{MTMT:34706689,
	title = {The human factor H protein family – an update},
	url = {https://m2.mtmt.hu/api/publication/34706689},
	author = {Sándor, Noémi and Schneider, Andrea E. and Matola, Alexandra Tünde and Barbai, Veronika H. and Bencze, Dániel and Hammad, Hani Hashim and Papp, Alexandra Éva and Kövesdi, Dorottya and Uzonyi, Barbara and Józsi, Mihály},
	doi = {10.3389/fimmu.2024.1135490},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {15},
	unique-id = {34706689},
	issn = {1664-3224},
	abstract = {Complement is an ancient and complex network of the immune system and, as such, it plays vital physiological roles, but it is also involved in numerous pathological processes. The proper regulation of the complement system is important to allow its sufficient and targeted activity without deleterious side-effects. Factor H is a major complement regulator, and together with its splice variant factor H-like protein 1 and the five human factor H-related (FHR) proteins, they have been linked to various diseases. The role of factor H in inhibiting complement activation is well studied, but the function of the FHRs is less characterized. Current evidence supports the main role of the FHRs as enhancers of complement activation and opsonization, i.e., counter-balancing the inhibitory effect of factor H. FHRs emerge as soluble pattern recognition molecules and positive regulators of the complement system. In addition, factor H and some of the FHR proteins were shown to modulate the activity of immune cells, a non-canonical function outside the complement cascade. Recent efforts have intensified to study factor H and the FHRs and develop new tools for the distinction, quantification and functional characterization of members of this protein family. Here, we provide an update and overview on the versatile roles of factor H family proteins, what we know about their biological functions in healthy conditions and in diseases.},
	year = {2024},
	eissn = {1664-3224},
	orcid-numbers = {Sándor, Noémi/0000-0002-2461-1389; Bencze, Dániel/0000-0001-7169-9515; Uzonyi, Barbara/0000-0002-9680-1974; Józsi, Mihály/0000-0002-5520-5535}
}

@article{MTMT:34267696,
	title = {Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display},
	url = {https://m2.mtmt.hu/api/publication/34267696},
	author = {Szöllősi, Dávid and Hajdrik, Polett and Tordai, Hedvig and Horváth, Ildikó and Veres, Dániel and Gillich, Bernadett and Shailaja, Kanni Das and Smeller, László and Bergmann, Ralf Konrad and Bachmann, Michael and Mihály, Judith and Gaál, Anikó and Jezsó, Bálint and Barátki, Balázs Lajos and Kövesdi, Dorottya and Bősze, Szilvia and Szabó, Ildikó and Felföldi, Tamás and Oszwald, Erzsébet and Padmanabhan, Parasuraman and Gulyás, Balázs Zoltán and Hamdani, Nazha and Máthé, Domokos and Varga, Zoltán and Szigeti, Krisztián},
	doi = {10.1038/s41598-023-45628-9},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {13},
	unique-id = {34267696},
	issn = {2045-2322},
	abstract = {The important roles of bacterial outer membrane vesicles (OMVs) in various diseases and their emergence as a promising platform for vaccine development and targeted drug delivery necessitates the development of imaging techniques suitable for quantifying their biodistribution with high precision. To address this requirement, we aimed to develop an OMV specific radiolabeling technique for positron emission tomography (PET). A novel bacterial strain ( E. coli BL21(DE3) ΔnlpI, ΔlpxM ) was created for efficient OMV production, and OMVs were characterized using various methods. SpyCatcher was anchored to the OMV outer membrane using autotransporter-based surface display systems. Synthetic SpyTag-NODAGA conjugates were tested for OMV surface binding and 64 Cu labeling efficiency. The final labeling protocol shows a radiochemical purity of 100% with a ~ 29% radiolabeling efficiency and excellent serum stability. The in vivo biodistribution of OMVs labeled with 64 Cu was determined in mice using PET/MRI imaging which revealed that the biodistribution of radiolabeled OMVs in mice is characteristic of previously reported data with the highest organ uptakes corresponding to the liver and spleen 3, 6, and 12 h following intravenous administration. This novel method can serve as a basis for a general OMV radiolabeling scheme and could be used in vaccine- and drug-carrier development based on bioengineered OMVs.},
	year = {2023},
	eissn = {2045-2322},
	orcid-numbers = {Szöllősi, Dávid/0000-0002-3363-3862; Hajdrik, Polett/0000-0002-5452-4892; Tordai, Hedvig/0000-0002-0875-5569; Veres, Dániel/0000-0002-9687-3556; Smeller, László/0000-0002-3643-3268; Gaál, Anikó/0000-0003-4064-1825; Jezsó, Bálint/0000-0002-1306-4797; Szabó, Ildikó/0000-0002-9844-7841; Felföldi, Tamás/0000-0003-2009-2478; Hamdani, Nazha/0000-0002-3053-0008; Varga, Zoltán/0000-0002-5741-2669}
}

@article{MTMT:34213739,
	title = {Factor H family proteins as modulators of complement activation for better or worse},
	url = {https://m2.mtmt.hu/api/publication/34213739},
	author = {Józsi, Mihály},
	journal-iso = {EUR J IMMUNOL},
	journal = {EUROPEAN JOURNAL OF IMMUNOLOGY},
	volume = {53},
	unique-id = {34213739},
	issn = {0014-2980},
	year = {2023},
	eissn = {1521-4141},
	pages = {38-38},
	orcid-numbers = {Józsi, Mihály/0000-0002-5520-5535}
}

@article{MTMT:34198665,
	title = {A novel monoclonal antibody reveals the enrichment of NADPH oxidase 5 in human splenic endothelial cells},
	url = {https://m2.mtmt.hu/api/publication/34198665},
	author = {Szeles, Zsolt and Petheő, Gábor L. and Szikora, Bence and Kacskovics, Imre and Geiszt, Miklós},
	doi = {10.1038/s41598-023-44018-5},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {13},
	unique-id = {34198665},
	issn = {2045-2322},
	abstract = {Members of the NOX/DUOX family of NADPH oxidases are responsible for regulated ROS production in diverse cells and tissues. Detection of NOX/DUOX proteins at the protein level remains an important challenge in the field. Here we report the development and characterization of a novel anti-NOX5 monoclonal antibody, which recognizes the human NOX5 protein in both Western blot, immunocytochemistry, and histochemistry applications. With the help of the antibody we could successfully detect both heterologously and endogenously expressed NOX5 in mammalian cells. Furthermore, we could also detect NOX5 protein in the human spleen, testis, and ovary. Immunohistochemical studies on human testis revealed that NOX5 localized to spermatogenic cells. This expression pattern was also supported by the result of in silico analysis of single-cell RNA sequencing data that indicated that NOX5 protein is present in developing spermatids and spermatocytes. Mature spermatozoa, however, did not contain detectable NOX5. In the human ovary, both immunostaining and single-cell RNA sequencing suggest that NOX5 is expressed in interstitial fibroblasts and theca cells. We also analyzed vascular cells for the presence of NOX5 and we found that NOX5 expression is a fairly specific feature of splenic endothelial cells.},
	year = {2023},
	eissn = {2045-2322},
	orcid-numbers = {Petheő, Gábor L./0000-0002-1773-4129; Szikora, Bence/0000-0002-0766-5684; Kacskovics, Imre/0000-0002-0402-3862}
}

@article{MTMT:34133958,
	title = {230 Interaction of factor H and factor H-related proteins with S and N proteins of SARS-CoV-2},
	url = {https://m2.mtmt.hu/api/publication/34133958},
	author = {Homonnayné Barbai, Veronika and Papp, Alexandra Éva and Bencze, Dániel and Uzonyi, Barbara and Józsi, Mihály},
	doi = {10.1016/j.imbio.2023.152680},
	journal-iso = {IMMUNOBIOLOGY},
	journal = {IMMUNOBIOLOGY},
	volume = {228},
	unique-id = {34133958},
	issn = {0171-2985},
	year = {2023},
	eissn = {1878-3279},
	orcid-numbers = {Bencze, Dániel/0000-0001-7169-9515; Uzonyi, Barbara/0000-0002-9680-1974; Józsi, Mihály/0000-0002-5520-5535}
}

@article{MTMT:34133943,
	title = {177 Factor H-related proteins bind to extracellular matrix components and affect complement activation},
	url = {https://m2.mtmt.hu/api/publication/34133943},
	author = {Papp, Alexandra Éva and Bencze, Dániel and Uzonyi, Barbara and Márquez-Tirado, Bárbara and de Jorge, Elena Goicoechea and Józsi, Mihály},
	doi = {10.1016/j.imbio.2023.152627},
	journal-iso = {IMMUNOBIOLOGY},
	journal = {IMMUNOBIOLOGY},
	volume = {228},
	unique-id = {34133943},
	issn = {0171-2985},
	year = {2023},
	eissn = {1878-3279},
	orcid-numbers = {Bencze, Dániel/0000-0001-7169-9515; Uzonyi, Barbara/0000-0002-9680-1974; Józsi, Mihály/0000-0002-5520-5535}
}

@article{MTMT:33999296,
	title = {Large-Scale Plasma Proteome Epitome Profiling is an Efficient Tool for the Discovery of Cancer Biomarkers},
	url = {https://m2.mtmt.hu/api/publication/33999296},
	author = {Lazar, Jozsef and Antal-Szalmás, Péter and Kurucz, Istvan and Ferenczi, Annamaria and Józsi, Mihály and Tornyi, Ilona and Muller, Monika and Fekete, János Tibor and Lamont, John and FitzGerald, Peter and Gall-Debreceni, Anna and Kádas, János and Vida, András and Tardieu, Nadege and Kieffer, Yann and Jullien, Anne and Guergova-Kuras, Mariana and Hempel, William and Kovacs, Andras and Kardos, Tamas and Bittner, Nóra and Csanky, Eszter and Szilasi, Mária and Losonczy, György and Szondy, Klára and Gálffy, Gabriella and Csada, Edit and Szalontai, Klára Margit and Somfay, Attila and Malka, David and Cottu, Paul and Bogos, Krisztina and Takacs, Laszlo},
	doi = {10.1016/j.mcpro.2023.100580},
	journal-iso = {MOL CELL PROTEOMICS},
	journal = {MOLECULAR & CELLULAR PROTEOMICS},
	volume = {22},
	unique-id = {33999296},
	issn = {1535-9476},
	year = {2023},
	eissn = {1535-9484},
	orcid-numbers = {Lazar, Jozsef/0000-0001-7337-3682; Józsi, Mihály/0000-0002-5520-5535; Fekete, János Tibor/0000-0002-6672-6563; Losonczy, György/0000-0002-5340-360X; Szondy, Klára/0000-0002-3717-3293; Somfay, Attila/0000-0001-5062-8152; Malka, David/0000-0002-7664-0130; Takacs, Laszlo/0000-0003-1764-2096}
}

@article{MTMT:33893006,
	title = {Liquid biopsy-based monitoring of residual disease in multiple myeloma by analysis of the rearranged immunoglobulin genes–A feasibility study},
	url = {https://m2.mtmt.hu/api/publication/33893006},
	author = {Marx, Anita and Osváth, Magdolna and Szikora, Bence and Pipek, Orsolya Anna and Csabai, István and Nagy, Ákos and Bödör, Csaba and Matula, Zsolt and Nagy, Ginette and Bors, András and Uher, Ferenc and Mikala, Gábor and Vályi-Nagy, István and Kacskovics, Imre},
	doi = {10.1371/journal.pone.0285696},
	journal-iso = {PLOS ONE},
	journal = {PLOS ONE},
	volume = {18},
	unique-id = {33893006},
	issn = {1932-6203},
	abstract = {The need for sensitive monitoring of minimal/measurable residual disease (MRD) in multiple myeloma emerged as novel therapies led to deeper responses. Moreover, the potential benefits of blood-based analyses, the so-called liquid biopsy is prompting more and more studies to assess its feasibility. Considering these recent demands, we aimed to optimize a highly sensitive molecular system based on the rearranged immunoglobulin (Ig) genes to monitor MRD from peripheral blood. We analyzed a small group of myeloma patients with the high-risk t(4;14) translocation, using next-generation sequencing of Ig genes and droplet digital PCR of patient-specific Ig heavy chain (IgH) sequences. Moreover, well established monitoring methods such as multiparametric flow cytometry and RT-qPCR of the fusion transcript IgH :: MMSET (IgH and multiple myeloma SET domain-containing protein) were utilized to evaluate the feasibility of these novel molecular tools. Serum measurements of M-protein and free light chains together with the clinical assessment by the treating physician served as routine clinical data. We found significant correlation between our molecular data and clinical parameters, using Spearman correlations. While the comparisons of the Ig-based methods and the other monitoring methods (flow cytometry, qPCR) were not statistically evaluable, we found common trends in their target detection. Regarding longitudinal disease monitoring, the applied methods yielded complementary information thus increasing the reliability of MRD evaluation. We also detected indications of early relapse before clinical signs, although this implication needs further verification in a larger patient cohort.},
	keywords = {multiple myeloma; liquid biopsy; circulating tumor DNA; next-generation sequencing (NGS); Circulating tumor cells; minimal residual disease (MRD); Droplet digital PCR (ddPCR); immunoglobulin genes},
	year = {2023},
	eissn = {1932-6203},
	orcid-numbers = {Marx, Anita/0000-0001-8969-5766; Szikora, Bence/0000-0002-0766-5684; Pipek, Orsolya Anna/0000-0001-8109-0340; Csabai, István/0000-0001-9232-9898; Bödör, Csaba/0000-0002-0729-692X; Bors, András/0000-0003-2109-4678; Uher, Ferenc/0000-0001-7997-6142; Kacskovics, Imre/0000-0002-0402-3862}
}

@article{MTMT:33786674,
	title = {Editorial: Updates on the complement system in kidney diseases},
	url = {https://m2.mtmt.hu/api/publication/33786674},
	author = {Bulla, Roberta and Józsi, Mihály},
	doi = {10.3389/fimmu.2023.1196487},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {14},
	unique-id = {33786674},
	issn = {1664-3224},
	year = {2023},
	eissn = {1664-3224},
	orcid-numbers = {Józsi, Mihály/0000-0002-5520-5535}
}

@article{MTMT:33726885,
	title = {The NF-κB Transcriptional Network Is a High-Dose Vitamin C-Targetable Vulnerability in Breast Cancer},
	url = {https://m2.mtmt.hu/api/publication/33726885},
	author = {Mussa, Ali and Afolabi, Hafeez Abiola and Syed, Nazmul Huda and Talib, Mustafa Mohammed Hamid and Murtadha, Ahmad Hafiz and Hajissa, Khalid and Mokhtar, Noor Fatmawati and Mohamud, Rohimah and Hassan, Rosline},
	doi = {10.3390/biomedicines11041060},
	journal-iso = {BIOMEDICINES},
	journal = {BIOMEDICINES},
	volume = {11},
	unique-id = {33726885},
	abstract = {Breast cancer (BC) is the most common cancer type among women with a distinct clinical presentation, but the survival rate remains moderate despite advances in multimodal therapy. Consequently, a deeper understanding of the molecular etiology is required for the development of more effective treatments for BC. The relationship between inflammation and tumorigenesis is well established, and the activation of the pro-inflammatory transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is frequently identified in BC. Constitutive NF-κB activation is linked to cell survival, metastasis, proliferation, and hormonal, chemo-, and radiotherapy resistance. Moreover, the crosstalk between NF-κB and other transcription factors is well documented. It is reported that vitamin C plays a key role in preventing and treating a number of pathological conditions, including cancer, when administered at remarkably high doses. Indeed, vitamin C can regulate the activation of NF-κB by inhibiting specific NF-κB-dependent genes and multiple stimuli. In this review, we examine the various NF-κB impacts on BC development. We also provide some insight into how the NF-κB network may be targeted as a potential vulnerability by using natural pro-oxidant therapies such as vitamin C.},
	year = {2023},
	eissn = {2227-9059},
	orcid-numbers = {Mussa, Ali/0000-0002-1116-7362; Afolabi, Hafeez Abiola/0000-0002-1120-4100; Talib, Mustafa Mohammed Hamid/0000-0002-0721-4314; Murtadha, Ahmad Hafiz/0000-0001-8580-9578; Hajissa, Khalid/0000-0002-1725-6702; Mokhtar, Noor Fatmawati/0000-0003-0349-4096; Mohamud, Rohimah/0000-0002-2465-6421; Hassan, Rosline/0000-0003-0493-1390}
}