@article{MTMT:34828140, title = {Lateral septum as a possible regulatory center of maternal behaviors}, url = {https://m2.mtmt.hu/api/publication/34828140}, author = {Puska, Gina and Szendi, Vivien and Dobolyi, Árpád}, doi = {10.1016/j.neubiorev.2024.105683}, journal-iso = {NEUROSCI BIOBEHAV R}, journal = {NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS}, volume = {161}, unique-id = {34828140}, issn = {0149-7634}, year = {2024}, eissn = {1873-7528}, orcid-numbers = {Dobolyi, Árpád/0000-0003-0397-2991} } @article{MTMT:34755849, title = {Sleep and local field potential effect of the D2 receptor agonist bromocriptine during the estrus cycle and postpartum period in female rats}, url = {https://m2.mtmt.hu/api/publication/34755849}, author = {Tóth, Attila István and Keserű, Dóra and Pethő, Máté and Détári, László and Bencsik, Norbert and Dobolyi, Árpád and Hajnik, Tünde}, doi = {10.1016/j.pbb.2024.173754}, journal-iso = {PHARMACOL BIOCHEM BE}, journal = {PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR}, volume = {239}, unique-id = {34755849}, issn = {0091-3057}, year = {2024}, eissn = {1873-5177}, orcid-numbers = {Tóth, Attila István/0000-0001-8607-8754; Détári, László/0000-0002-0630-8466; Bencsik, Norbert/0000-0001-9553-3851; Dobolyi, Árpád/0000-0003-0397-2991; Hajnik, Tünde/0000-0003-0684-8136} } @article{MTMT:34755053, title = {Sleep- and sleep deprivation-related changes of vertex auditory evoked potentials during the estrus cycle in female rats}, url = {https://m2.mtmt.hu/api/publication/34755053}, author = {Tóth, Attila István and Traub, Máté and Bencsik, Norbert and Détári, László and Hajnik, Tünde and Dobolyi, Árpád}, doi = {10.1038/s41598-024-56392-9}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {14}, unique-id = {34755053}, issn = {2045-2322}, abstract = {The estrus cycle in female rodents has been shown to affect a variety of physiological functions. However, little is known about its presumably thorough effect on auditory processing during the sleep–wake cycle and sleep deprivation. Vertex auditory evoked potentials (vAEPs) were evoked by single click tone stimulation and recorded during different stages of the estrus cycle and sleep deprivation performed in metestrus and proestrus in female rats. vAEPs showed a strong sleep-dependency, with the largest amplitudes present during slow wave sleep while the smallest ones during wakefulness. Higher amplitudes and longer latencies were seen in the light phase during all vigilance stages. The largest amplitudes were found during proestrus (light phase) while the shortest latencies were seen during estrus (dark phase) compared to the 2nd day diestrus baseline. High-amplitude responses without latency changes were also seen during metestrus with increased homeostatic sleep drive. More intense and faster processing of auditory information during proestrus and estrus suggesting a more effective perception of relevant environmental cues presumably in preparation for sexual receptivity. A 4-h sleep deprivation resulted in more pronounced sleep recovery in metestrus compared to proestrus without difference in delta power replacement suggesting a better tolerance of sleep deprivation in proestrus. Sleep deprivation decreased neuronal excitability and responsiveness in a similar manner both during metestrus and proestrus, suggesting that the negative consequences of sleep deprivation on auditory processing may have a limited correlation with the estrus cycle stage.}, year = {2024}, eissn = {2045-2322}, orcid-numbers = {Tóth, Attila István/0000-0001-8607-8754; Bencsik, Norbert/0000-0001-9553-3851; Détári, László/0000-0002-0630-8466; Hajnik, Tünde/0000-0003-0684-8136; Dobolyi, Árpád/0000-0003-0397-2991} } @misc{MTMT:34718533, title = {Cell-type specific effects of Fusarium mycotoxins on primary neuronal and astroglial cells}, url = {https://m2.mtmt.hu/api/publication/34718533}, author = {Viktória, Szentgyörgyi and Bencsik, Norbert and Brigitta, Micska and Rátkai, Erika Anikó and Schlett, Katalin and Tárnok, Krisztián}, unique-id = {34718533}, abstract = {Fumonisin B1, deoxynivalenol (DON) and zearalenone (ZEA) are toxic secondary metabolites produced by Fusarium molds. These mycotoxins are common food and feed pollutants and represent a risk for human and animal health. Although the mycotoxins produced by this genus can cross the blood-brain-barrier (BBB) in many species, their effect on neuronal function remains unclear. We investigated cell viability effects of these toxins on specified neural cell types, including mouse primary neuronal, astroglial and mixed cell cultures 24 or 48 hours after mycotoxin administration. Cell viability assay revealed that DON decreased cell viability in a dose-dependent manner, independently from the culture's type. Fumonisin B1 increased cell viability significantly on astroglial and mixed cell cultures in lower doses, while it exerted a highly toxic effect in 50 μM. ZEA had significant effects on all culture type in 10 nM by increasing the cell viability. Since ZEA is a mycoestrogen, we analyzed the effects of ZEA on the expression of estrogen receptor isotypes ERα and ERβ and mitochondrial voltage-dependent anion channel (VDAC1) by qRT-PCR. In neuronal and mixed cultures, ZEA administration decreased ERα expression, while in astroglial cultures, it induced the opposite effect. ERβ and VDAC1 expression was not altered by ZEA in either culture types. ZEA also affected the firing pattern of neurons by enhancing the burst frequency. Our results demonstrate that Fusarium mycotoxins are acting on a cell specific manner in the brain tissue.Competing Interest StatementThe authors have declared no competing interest.}, year = {2024}, pages = {2024.02.29.582764}, orcid-numbers = {Viktória, Szentgyörgyi/0000-0003-2827-4399; Bencsik, Norbert/0000-0001-9553-3851; Schlett, Katalin/0000-0001-9265-4236; Tárnok, Krisztián/0000-0003-3491-4014} } @misc{MTMT:34685806, title = {Redox-dependent condensation and cytoplasmic granulation by human ssDNA binding protein 1 delineate roles in oxidative stress response}, url = {https://m2.mtmt.hu/api/publication/34685806}, author = {Harami, Gábor and János, Pálinkás and Kovács, Zoltán and Jezsó, Bálint and Tárnok, Krisztián and Hajnalka, Harami-Papp and József, Hegedüs and Lamiya, Mahmudova and Nóra, Kucsma and Szilárd, Tóth and Gergely, Szakács and Kovács, Mihály}, unique-id = {34685806}, year = {2024}, orcid-numbers = {Harami, Gábor/0000-0001-7234-2710; Jezsó, Bálint/0000-0002-1306-4797; Tárnok, Krisztián/0000-0003-3491-4014; Kovács, Mihály/0000-0002-1200-4741} } @article{MTMT:34497633, title = {Comparative analysis of hippocampal extracellular space uncovers widely altered peptidome upon epileptic seizure in urethane-anaesthetized rats}, url = {https://m2.mtmt.hu/api/publication/34497633}, author = {Tukacs, Vanda and Mittli, Dániel Árpád and Hunyadi-Gulyás Éva, Csilla and Darula, Zsuzsanna and Juhász, Gábor Dénes and Kardos, József and Kékesi, Adrienna Katalin}, doi = {10.1186/s12987-024-00508-w}, journal-iso = {FLUIDS BARRIERS CNS}, journal = {FLUIDS AND BARRIERS OF THE CNS}, volume = {21}, unique-id = {34497633}, issn = {2045-8118}, abstract = {Background: The brain extracellular fluid (ECF), composed of secreted neurotransmitters, metabolites, peptides, and proteins, may reflect brain processes. Analysis of brain ECF may provide new potential markers for synaptic activity or brain damage and reveal additional information on pathological alterations. Epileptic seizure induction is an acute and harsh intervention in brain functions, and it can activate extra- and intracellular proteases, which implies an altered brain secretome. Thus, we applied a 4-aminopyridine (4-AP) epilepsy model to study the hippocampal ECF peptidome alterations upon treatment in rats. Methods: We performed in vivo microdialysis in the hippocampus for 3–3 h of control and 4-AP treatment phase in parallel with electrophysiology measurement. Then, we analyzed the microdialysate peptidome of control and treated samples from the same subject by liquid chromatography-coupled tandem mass spectrometry. We analyzed electrophysiological and peptidomic alterations upon epileptic seizure induction by two-tailed, paired t-test. Results: We detected 2540 peptides in microdialysate samples by mass spectrometry analysis; and 866 peptides—derived from 229 proteins—were found in more than half of the samples. In addition, the abundance of 322 peptides significantly altered upon epileptic seizure induction. Several proteins of significantly altered peptides are neuropeptides (Chgb) or have synapse- or brain-related functions such as the regulation of synaptic vesicle cycle (Atp6v1a, Napa), astrocyte morphology (Vim), and glutamate homeostasis (Slc3a2). Conclusions: We have detected several consequences of epileptic seizures at the peptidomic level, as altered peptide abundances of proteins that regulate epilepsy-related cellular processes. Thus, our results indicate that analyzing brain ECF by in vivo microdialysis and omics techniques is useful for monitoring brain processes, and it can be an alternative method in the discovery and analysis of CNS disease markers besides peripheral fluid analysis. © 2024, The Author(s).}, keywords = {Animals; Male; PEPTIDES; metabolism; PEPTIDE; SEIZURES; comparative study; amino acid sequence; Extracellular Space; nonhuman; animal model; animal experiment; pathology; slow brain wave; Electroencephalogram; urethan; glial fibrillary acidic protein; area under the curve; in vivo study; seizure; AMIDES; AMIDE; upregulation; brain function; neuropeptide Y; ASTROCYTE; tandem mass spectrometry; Temporal lobe epilepsy; Urethane; MICRODIALYSIS; synapse vesicle; FAMPRIDINE; peptidomics; Gene set enrichment analysis; rat; secretoneurin; Chromogranin B}, year = {2024}, eissn = {2045-8118}, orcid-numbers = {Juhász, Gábor Dénes/0000-0002-0849-6931; Kardos, József/0000-0002-2135-2932; Kékesi, Adrienna Katalin/0000-0003-3042-4878} } @article{MTMT:34472863, title = {Valproate treatment induces age‐ and sex‐dependent neuronal activity changes according to a patch clamp study}, url = {https://m2.mtmt.hu/api/publication/34472863}, author = {Májer, Tímea and Bódi, Veronika and Kelemen, Viktor and Szűcs, Attila and Varró, Petra and Világi, Ildikó}, doi = {10.1002/dneu.22933}, journal-iso = {DEV NEUROBIOL}, journal = {DEVELOPMENTAL NEUROBIOLOGY}, volume = {84}, unique-id = {34472863}, issn = {1932-8451}, abstract = {Autism spectrum disorder is a heterogeneous neurodevelopmental disorder characterized by impaired social interactions, restricted, and stereotyped behaviors. The valproic acid model is one of the most recognized and broadly used models in rats to induce core symptoms of this disorder. Comorbidity of epilepsy and autism occurs frequently, due to similar background mechanisms that include the imbalance of excitation and inhibition. In this series of experiments, treatment was performed on rat dams with a single 500 mg/kg dose i.p. valproate injection on embryonic day 12.5. Intracellular whole‐cell patch clamp recordings were performed on brain slices prepared from adolescent and adult offspring of both sexes on pyramidal neurons of the medial prefrontal cortex and entorhinal cortex. Current clamp stimulation utilizing conventional current step protocols and dynamic clamp stimulation were applied to assess neuronal excitability. Membrane properties and spiking characteristics of layer II–III pyramidal cells were analyzed in both cortical regions. Significant sex‐dependent and age‐dependent differences were found in several parameters in the control groups. Considering membrane resistance, rheobase, voltage sag slope, and afterdepolarization slope, we observed notable changes mainly in the female groups. Valproate treatment seemed to enhance these differences and increase network excitability. However, it is possible that compensatory mechanisms took place during the maturation of the network while reaching the age‐group of 3 months. Based on the results, the expression of the hyperpolarization‐activated cyclic nucleotide‐gated channels may be appreciably affected by the valproate treatment, which influences fundamental electrophysiological properties of the neurons such as the voltage sag. Remarkable changes appeared in the prefrontal cortex; however, also the entorhinal cortex shows similar tendencies.}, year = {2024}, eissn = {1932-846X}, pages = {32-43}, orcid-numbers = {Szűcs, Attila/0000-0001-9733-4135; Varró, Petra/0000-0001-7945-2672; Világi, Ildikó/0000-0002-1301-6568} } @misc{MTMT:34694668, title = {Multifactorial approach is needed to unravel the maturation phases of human neurons derived from induced pluripotent stem cells}, url = {https://m2.mtmt.hu/api/publication/34694668}, author = {Ben Mahmoud, Maissa and Rátkai, Erika Anikó and Krisztina, Bauer and Bencsik, Norbert and Szűcs, Attila and Schlett, Katalin and Tárnok, Krisztián}, unique-id = {34694668}, abstract = {Neurons derived from induced pluripotent stem cells (h-iPSC-Ns) provide an invaluable model for studying the physiological aspects of neuronal development and diseases. However, multiple studies have also demonstrated that h-iPSC-Ns exhibit a high degree of functional and epigenetic diversity. Due to the imprecise characterization and significant variation among the currently available maturation protocols, it is essential to establish a set of criteria to standardize models and accurately characterize and define the developmental properties of neurons derived from iPSCs.In this study, we conducted a comprehensive analysis of the h-iPSC-Ns via electrophysiological and microscopic techniques to follow their functional development at the cellular and network levels. This enabled us to provide a thorough description of the maturation process of h-iPSC-Ns over a 10-week period in vitro. Specifically, we have used conventional whole-cell patch-clamp and dynamic clamp techniques, alongside morphometry, to assess the characteristics of maturing h-iPSC-Ns. Additionally, we utilized calcium imaging to monitor the progression of synaptic activity and network communication. At the single cell level, human neurons exhibited gradually decreasing membrane resistance in parallel with improved excitability by 5 weeks of maturation. Their firing profiles were consistent with those of mature regular firing type of neurons. At the network level we observed the development of abundant fast glutamatergic and depolarizing GABAergic synaptic connections together with synchronized network activity. The identified sequence of differentiation events are consistent and offers a robust framework for developing targeted experiments at varying stages of neuronal maturation. This framework allows for the use of different, age-related methodologies or a singular set of experiments for a culture’s maturation.Competing Interest StatementThe authors have declared no competing interest.}, year = {2023}, pages = {2023.09.21.558836}, orcid-numbers = {Ben Mahmoud, Maissa/0000-0003-0931-915X; Bencsik, Norbert/0000-0001-9553-3851; Szűcs, Attila/0000-0001-9733-4135; Schlett, Katalin/0000-0001-9265-4236; Tárnok, Krisztián/0000-0003-3491-4014} } @article{MTMT:34422513, title = {Inflammatory processes in the prefrontal cortex induced by systemic immune challenge: Focusing on neurons}, url = {https://m2.mtmt.hu/api/publication/34422513}, author = {Mittli, Dániel Árpád}, doi = {10.1016/j.bbih.2023.100703}, journal-iso = {BRAIN BEHAVIOR IMMUNITY - HEALTH}, journal = {BRAIN BEHAVIOR IMMUNITY - HEALTH}, volume = {34}, unique-id = {34422513}, year = {2023}, eissn = {2666-3546} } @article{MTMT:34317854, title = {Risk and protective factors for health behaviour in adolescence in Europe}, url = {https://m2.mtmt.hu/api/publication/34317854}, author = {Zsákai, Annamária and Rátz-Sulyok, Fanny Zselyke and Koronczai, Beatrix and Varró, Petra and M Tóth, Erika and Szarvas, S. and Tauber, T. and Karkus, Z. and Molnár, Kinga}, doi = {10.1038/s41598-023-45800-1}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {13}, unique-id = {34317854}, issn = {2045-2322}, year = {2023}, eissn = {2045-2322}, orcid-numbers = {Zsákai, Annamária/0000-0001-8880-2056; Koronczai, Beatrix/0000-0003-0884-1798; Varró, Petra/0000-0001-7945-2672; M Tóth, Erika/0000-0001-9048-5758; Molnár, Kinga/0000-0002-7196-5331} }