TY - JOUR AU - Balog, József Ágoston AU - Zvara, Ágnes AU - Bukovinszki, Vivien AU - Puskás, László AU - Balog, Attila AU - Szebeni, Gábor TI - Comparative single-cell multiplex immunophenotyping of therapy-naive patients with rheumatoid arthritis, systemic sclerosis, and systemic lupus erythematosus shed light on disease-specific composition of the peripheral immune system JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 15 PY - 2024 SN - 1664-3224 DO - 10.3389/fimmu.2024.1376933 UR - https://m2.mtmt.hu/api/publication/34829278 ID - 34829278 LA - English DB - MTMT ER - TY - JOUR AU - Faragó, Anna AU - Zvara, Ágnes AU - Tiszlavicz, László AU - Hunyadi-Gulyás Éva, Csilla AU - Darula, Zsuzsanna AU - Hegedűs, Zoltán AU - Szabó, Enikő AU - Surguta, Sára Eszter AU - Tóvári, József AU - Puskás, László AU - Szebeni, Gábor TI - Lectin-Based Immunophenotyping and Whole Proteomic Profiling of CT-26 Colon Carcinoma Murine Model. JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 7 PG - 21 SN - 1661-6596 DO - 10.3390/ijms25074022 UR - https://m2.mtmt.hu/api/publication/34790193 ID - 34790193 N1 - * Megosztott szerzőség AB - A murine colorectal carcinoma (CRC) model was established. CT26 colon carcinoma cells were injected into BALB/c mice's spleen to study the primary tumor and the mechanisms of cell spread of colon cancer to the liver. The CRC was verified by the immunohistochemistry of Pan Cytokeratin and Vimentin expression. Immunophenotyping of leukocytes isolated from CRC-bearing BALB/c mice or healthy controls, such as CD19+ B cells, CD11+ myeloid cells, and CD3+ T cells, was carried out using fluorochrome-labeled lectins. The binding of six lectins to white blood cells, such as galectin-1 (Gal1), siglec-1 (Sig1), Sambucus nigra lectin (SNA), Aleuria aurantia lectin (AAL), Phytolacca americana lectin (PWM), and galectin-3 (Gal3), was assayed. Flow cytometric analysis of the splenocytes revealed the increased binding of SNA, and AAL to CD3 + T cells and CD11b myeloid cells; and increased siglec-1 and AAL binding to CD19 B cells of the tumor-bearing mice. The whole proteomic analysis of the established CRC-bearing liver and spleen versus healthy tissues identified differentially expressed proteins, characteristic of the primary or secondary CRC tissues. KEGG Gene Ontology bioinformatic analysis delineated the established murine CRC characteristic protein interaction networks, biological pathways, and cellular processes involved in CRC. Galectin-1 and S100A4 were identified as upregulated proteins in the primary and secondary CT26 tumor tissues, and these were previously reported to contribute to the poor prognosis of CRC patients. Modelling the development of liver colonization of CRC by the injection of CT26 cells into the spleen may facilitate the understanding of carcinogenesis in human CRC and contribute to the development of novel therapeutic strategies. LA - English DB - MTMT ER - TY - JOUR AU - Kant, Kamal AU - Rigó, Gábor AU - Faragó, Dóra AU - Benyó, Dániel AU - Tengölics, Roland AU - Szabados, László AU - Zsigmond, Laura TI - Mutation in Arabidopsis mitochondrial Pentatricopeptide repeat 40 gene affects tolerance to water deficit JF - PLANTA J2 - PLANTA VL - 259 PY - 2024 IS - 4 SN - 0032-0935 DO - 10.1007/s00425-024-04354-w UR - https://m2.mtmt.hu/api/publication/34721641 ID - 34721641 N1 - Funding Agency and Grant Number: HUN-REN Biological Research Centre, Szeged Funding text: Open access funding provided by HUN-REN Biological Research Centre, Szeged. LA - English DB - MTMT ER - TY - JOUR AU - Schaubmayr, W. AU - Hochreiter, B. AU - Hunyadi-Gulyás Éva, Csilla AU - Riegler, L. AU - Schmidt, K. AU - Tiboldi, A. AU - Moser, B. AU - Klein, K.U. AU - Krenn, K. AU - Scharbert, G. AU - Mohr, T. AU - Schmid, J.A. AU - Spittler, A. AU - Tretter, V. TI - The Proteome of Extracellular Vesicles Released from Pulmonary Microvascular Endothelium Reveals Impact of Oxygen Conditions on Biotrauma JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 4 SN - 1661-6596 DO - 10.3390/ijms25042415 UR - https://m2.mtmt.hu/api/publication/34721386 ID - 34721386 N1 - Department of Anesthesia, General Intensive Care and Pain Medicine, Medical University of Vienna, Vienna, 1090, Austria Laboratory of Proteomics Research, HUN-REN Biological Research Centre, Szeged, 6726, Hungary Department of Orthopedics and Trauma Surgery, Medical University of Vienna, Vienna, 1090, Austria Core Facility of Cell Imaging and Ultrastructure Research, University of Vienna, Vienna, 1090, Austria Department of Thoracic Surgery, Medical University of Vienna, Vienna, 1090, Austria Institute of Cancer Research, Department of Medicine I, Comprehensive Cancer Center, Medical University of Vienna, Vienna, 1090, Austria Institute of Vascular Biology and Thrombosis Research, Medical University of Vienna, Vienna, 1090, Austria Department of Surgery and Core Facility Flow Cytometry, Medical University of Vienna, Vienna, 1090, Austria Export Date: 5 March 2024 Correspondence Address: Tretter, V.; Department of Anesthesia, Austria; email: verena.tretter@meduniwien.ac.at Funding details: 739593 Funding details: Austrian Science Fund, FWF, P28618-B28 Funding text 1: The work of the research team at Medical University Vienna was supported by the Austrian Science Fund (FWF P28618-B28 to K.U.K.), funds from Apeptico Forschung und Entwicklung GmbH and Alterras Therapeutics GmbH to K.K. and funds from the Department of Anesthesia, General Intensive Care and Pain Therapy. HCEMM has received funding from the EU’s Horizon 2020 research and innovation program under grant agreement No. 739593. AB - The lung can experience different oxygen concentrations, low as in hypoxia, high as under supplemental oxygen therapy, or oscillating during intermittent hypoxia as in obstructive sleep apnea or intermittent hypoxia/hyperoxia due to cyclic atelectasis in the ventilated patient. This study aimed to characterize the oxygen-condition-specific protein composition of extracellular vesicles (EVs) released from human pulmonary microvascular endothelial cells in vitro to decipher their potential role in biotrauma using quantitative proteomics with bioinformatic evaluation, transmission electron microscopy, flow cytometry, and non-activated thromboelastometry (NATEM). The release of vesicles enriched in markers CD9/CD63/CD81 was enhanced under intermittent hypoxia, strong hyperoxia and intermittent hypoxia/hyperoxia. Particles with exposed phosphatidylserine were increased under intermittent hypoxia. A small portion of vesicles were tissue factor-positive, which was enhanced under intermittent hypoxia and intermittent hypoxia/hyperoxia. EVs from treatment with intermittent hypoxia induced a significant reduction of Clotting Time in NATEM analysis compared to EVs isolated after normoxic exposure, while after intermittent hypoxia/hyperoxia, tissue factor in EVs seems to be inactive. Gene set enrichment analysis of differentially expressed genes revealed that EVs from individual oxygen conditions potentially induce different biological processes such as an inflammatory response under strong hyperoxia and intermittent hypoxia/hyperoxia and enhancement of tumor invasiveness under intermittent hypoxia. © 2024 by the authors. LA - English DB - MTMT ER - TY - JOUR AU - Tukacs, Vanda AU - Mittli, Dániel Árpád AU - Hunyadi-Gulyás Éva, Csilla AU - Darula, Zsuzsanna AU - Juhász, Gábor Dénes AU - Kardos, József AU - Kékesi, Adrienna Katalin TI - Comparative analysis of hippocampal extracellular space uncovers widely altered peptidome upon epileptic seizure in urethane-anaesthetized rats JF - FLUIDS AND BARRIERS OF THE CNS J2 - FLUIDS BARRIERS CNS VL - 21 PY - 2024 IS - 1 SN - 2045-8118 DO - 10.1186/s12987-024-00508-w UR - https://m2.mtmt.hu/api/publication/34497633 ID - 34497633 N1 - ELTE NAP Neuroimmunology Research Group, Department of Biochemistry, Institute of Biology, ELTE Eötvös Loránd University, Pázmány Péter Sétány 1/C, Budapest, 1117, Hungary Laboratory of Proteomics, Institute of Biology, ELTE Eötvös Loránd University, Pázmány Péter Sétány 1/C, Budapest, 1117, Hungary Laboratory of Proteomics Research, Biological Research Centre, Hungarian Research Network (HUN-REN), Temesvári Körút 62, Szeged, 6726, Hungary Single Cell Omics Advanced Core Facility, Hungarian Centre of Excellence for Molecular Medicine, Temesvári Körút 62, Szeged, 6726, Hungary InnoScience Hungary Ltd., Bátori Út 9, Mátranovák, 3142, Hungary Department of Physiology and Neurobiology, Institute of Biology, ELTE Eötvös Loránd University, Pázmány Péter Sétány 1/C, Budapest, 1117, Hungary Export Date: 01 February 2024; Cited By: 0; Correspondence Address: K.A. Kékesi; ELTE NAP Neuroimmunology Research Group, Department of Biochemistry, Institute of Biology, ELTE Eötvös Loránd University, Budapest, Pázmány Péter Sétány 1/C, 1117, Hungary; email: kakekesi@ttk.elte.hu AB - Background: The brain extracellular fluid (ECF), composed of secreted neurotransmitters, metabolites, peptides, and proteins, may reflect brain processes. Analysis of brain ECF may provide new potential markers for synaptic activity or brain damage and reveal additional information on pathological alterations. Epileptic seizure induction is an acute and harsh intervention in brain functions, and it can activate extra- and intracellular proteases, which implies an altered brain secretome. Thus, we applied a 4-aminopyridine (4-AP) epilepsy model to study the hippocampal ECF peptidome alterations upon treatment in rats. Methods: We performed in vivo microdialysis in the hippocampus for 3–3 h of control and 4-AP treatment phase in parallel with electrophysiology measurement. Then, we analyzed the microdialysate peptidome of control and treated samples from the same subject by liquid chromatography-coupled tandem mass spectrometry. We analyzed electrophysiological and peptidomic alterations upon epileptic seizure induction by two-tailed, paired t-test. Results: We detected 2540 peptides in microdialysate samples by mass spectrometry analysis; and 866 peptides—derived from 229 proteins—were found in more than half of the samples. In addition, the abundance of 322 peptides significantly altered upon epileptic seizure induction. Several proteins of significantly altered peptides are neuropeptides (Chgb) or have synapse- or brain-related functions such as the regulation of synaptic vesicle cycle (Atp6v1a, Napa), astrocyte morphology (Vim), and glutamate homeostasis (Slc3a2). Conclusions: We have detected several consequences of epileptic seizures at the peptidomic level, as altered peptide abundances of proteins that regulate epilepsy-related cellular processes. Thus, our results indicate that analyzing brain ECF by in vivo microdialysis and omics techniques is useful for monitoring brain processes, and it can be an alternative method in the discovery and analysis of CNS disease markers besides peripheral fluid analysis. © 2024, The Author(s). LA - English DB - MTMT ER - TY - JOUR AU - Sharma, Sumit AU - Shukla, Siddharth AU - Rawal, Amit AU - Jee, Shyam AU - Ayaydin, Ferhan AU - Vásárhelyi, Lívia AU - Kukovecz, Ákos AU - Kumar, Vijay AU - Kadi, Nawar TI - Droplet navigation on metastable hydrophobic and superhydrophobic nonwoven materials JF - COLLOIDS AND SURFACES A : PHYSICOCHEMICAL AND ENGINEERING ASPECTS J2 - COLLOID SURFACE A VL - 683 PY - 2024 SP - 132993 SN - 0927-7757 DO - 10.1016/j.colsurfa.2023.132993 UR - https://m2.mtmt.hu/api/publication/34491425 ID - 34491425 LA - English DB - MTMT ER - TY - JOUR AU - Tengölics, Roland AU - Szappanos, Balázs AU - Mülleder, M AU - Kalapis, Dorottya AU - Grézal, Gábor AU - Sajben, Cs AU - Agostini, F AU - Mokochinski, Joao Benhur AU - Bálint, Balázs AU - Nagy, LG AU - Ralser, M AU - Papp, Balázs TI - The metabolic domestication syndrome of budding yeast JF - PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA J2 - P NATL ACAD SCI USA VL - 121 PY - 2024 IS - 11 PG - 11 SN - 0027-8424 DO - 10.1073/pnas.2313354121 UR - https://m2.mtmt.hu/api/publication/34479463 ID - 34479463 LA - English DB - MTMT ER - TY - JOUR AU - Tóth, Dávid AU - Kuntam, Soujanya AU - Ferenczi, Áron AU - Vidal-Meireles, Andre AU - Kovács, László AU - Wang, Lianyong AU - Sarkadi, Zsuzsa AU - Migh, Ede AU - Szentmihályi, Klára AU - Tengölics, Roland AU - Neupert, Juliane AU - Bock, Ralph AU - Jonikas, Martin C AU - Molnar, Attila AU - Tóth, Szilvia Zita TI - Chloroplast phosphate transporter CrPHT4-7 regulates phosphate homeostasis and photosynthesis in Chlamydomonas JF - PLANT PHYSIOLOGY J2 - PLANT PHYSIOL VL - 194 PY - 2024 IS - 3 SP - 1646 EP - 1661 PG - 16 SN - 0032-0889 DO - 10.1093/plphys/kiad607 UR - https://m2.mtmt.hu/api/publication/34435877 ID - 34435877 N1 - Funding Agency and Grant Number: Lendulet/Momentum Programme of the Hungarian Academy of Sciences [LP2014/19]; National Research, Development, and Innovation Office [K132600]; Biotechnology and Biological Sciences Research Council (BBSRC) [BB/R506163/1]; U.S. Department of Energy [DE-SC0020195]; Max Planck Society Funding text: This work was supported by the Lendulet/Momentum Programme of the Hungarian Academy of Sciences (LP2014/19 research grant to S.Z.T.) and the National Research, Development, and Innovation Office (K132600 research grant to S.Z.T.). A.F. was supported by Biotechnology and Biological Sciences Research Council (BBSRC) grant BB/R506163/1. L.W. and M.J. were supported by U.S. Department of Energy grant DE-SC0020195. M.J. is an investigator of the Howard Hughes Medical Institute. J.N. and R.B. were supported by the Max Planck Society. AB - In eukaryotic cells, phosphorus is assimilated and utilized primarily as phosphate (Pi). Pi homeostasis is mediated by transporters that have not yet been adequately characterized in green algae. This study reports on PHOSPHATE TRANSPORTER 4-7 (CrPHT4-7) from Chlamydomonas reinhardtii, a member of the PHT4 transporter family, which exhibits remarkable similarity to AtPHT4;4 from Arabidopsis (Arabidopsis thaliana), a chloroplastic ascorbate transporter. Using fluorescent protein tagging, we show that CrPHT4-7 resides in the chloroplast envelope membrane. Crpht4-7 mutants, generated by the CRISPR/Cas12a-mediated single-strand templated repair, show retarded growth, especially in high light, reduced ATP level, strong ascorbate accumulation, and diminished non-photochemical quenching in high light. On the other hand, total cellular phosphorous content was unaffected, and the phenotype of the Crpht4-7 mutants could not be alleviated by ample Pi supply. CrPHT4-7-overexpressing lines exhibit enhanced biomass accumulation under high light conditions in comparison with the wild-type strain. Expressing CrPHT4-7 in a yeast (Saccharomyces cerevisiae) strain lacking Pi transporters substantially recovered its slow growth phenotype, demonstrating that CrPHT4-7 transports Pi. Even though CrPHT4-7 shows a high degree of similarity to AtPHT4;4, it does not display any substantial ascorbate transport activity in yeast or intact algal cells. Thus, the results demonstrate that CrPHT4-7 functions as a chloroplastic Pi transporter essential for maintaining Pi homeostasis and photosynthesis in C. reinhardtii. LA - English DB - MTMT ER - TY - JOUR AU - Gémes, Nikolett AU - Balog, József Ágoston AU - Neuperger, Patricia AU - Schlegl, Erzsébet AU - Barta, Imre AU - Fillinger, János AU - Antus, Balázs AU - Zvara, Ágnes AU - Hegedűs, Zoltán AU - Czimmerer, Zsolt AU - Manczinger, Máté AU - Balogh, Gergő Mihály AU - Tóvári, József AU - Puskás, László AU - Szebeni, Gábor TI - Single-cell immunophenotyping revealed the association of CD4+ central and CD4+ effector memory T cells linking exacerbating chronic obstructive pulmonary disease and NSCLC. JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 14 PY - 2023 PG - 16 SN - 1664-3224 DO - 10.3389/fimmu.2023.1297577 UR - https://m2.mtmt.hu/api/publication/34486293 ID - 34486293 N1 - * Megosztott szerzőség AB - Tobacco smoking generates airway inflammation in chronic obstructive pulmonary disease (COPD), and its involvement in the development of lung cancer is still among the leading causes of early death. Therefore, we aimed to have a better understanding of the disbalance in immunoregulation in chronic inflammatory conditions in smoker subjects with stable COPD (stCOPD), exacerbating COPD (exCOPD), or non-small cell lung cancer (NSCLC).Smoker controls without chronic illness were recruited as controls. Through extensive mapping of single cells, surface receptor quantification was achieved by single-cell mass cytometry (CyTOF) with 29 antibodies. The CyTOF characterized 14 main immune subsets such as CD4+, CD8+, CD4+/CD8+, CD4-/CD8-, and γ/δ T cells and other subsets such as CD4+ or CD8+ NKT cells, NK cells, B cells, plasmablasts, monocytes, CD11cdim, mDCs, and pDCs. The CD4+ central memory (CM) T cells (CD4+/CD45RA-/CD45RO+/CD197+) and CD4+ effector memory (EM) T cells (CD4+/CD45RA-/CD45RO+/CD197-) were FACS-sorted for RNA-Seq analysis. Plasma samples were assayed by Luminex MAGPIX® for the quantitative measurement of 17 soluble immuno-oncology mediators (BTLA, CD28, CD80, CD27, CD40, CD86, CTLA-4, GITR, GITRL, HVEM, ICOS, LAG-3, PD-1, PD-L1, PD-L2, TIM-3, TLR-2) in the four studied groups.Our focus was on T-cell-dependent differences in COPD and NSCLC, where peripheral CD4+ central memory and CD4+ effector memory cells showed a significant reduction in exCOPD and CD4+ CM showed elevation in NSCLC. The transcriptome analysis delineated a perfect correlation of differentially expressed genes between exacerbating COPD and NSCLC-derived peripheral CD4+ CM or CD4+ EM cells. The measurement of 17 immuno-oncology soluble mediators revealed a disease-associated phenotype in the peripheral blood of stCOPD, exCOPD, and NSCLC patients.The applied single-cell mass cytometry, the whole transcriptome profiling of peripheral CD4+ memory cells, and the quantification of 17 plasma mediators provided complex data that may contribute to the understanding of the disbalance in immune homeostasis generated or sustained by tobacco smoking in COPD and NSCLC. LA - English DB - MTMT ER - TY - JOUR AU - Cinege, Gyöngyi Ilona AU - Magyar, Lilla Brigitta AU - Kovács, Henrietta AU - Varga, Viktória AU - Bodai, László AU - Zsindely, Nóra AU - Nagy, Gábor AU - Hegedűs, Zoltán AU - Hultmark, Dan AU - Andó, István TI - Distinctive features of Zaprionus indianus hemocyte differentiation and function revealed by transcriptomic analysis JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 14 PY - 2023 PG - 14 SN - 1664-3224 DO - 10.3389/fimmu.2023.1322381 UR - https://m2.mtmt.hu/api/publication/34446740 ID - 34446740 N1 - * Megosztott szerzőség LA - English DB - MTMT ER -