@article{MTMT:34829278,
	title = {Comparative single-cell multiplex immunophenotyping of therapy-naive patients with rheumatoid arthritis, systemic sclerosis, and systemic lupus erythematosus shed light on disease-specific composition of the peripheral immune system},
	url = {https://m2.mtmt.hu/api/publication/34829278},
	author = {Balog, József Ágoston and Zvara, Ágnes and Bukovinszki, Vivien and Puskás, László and Balog, Attila and Szebeni, Gábor},
	doi = {10.3389/fimmu.2024.1376933},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {15},
	unique-id = {34829278},
	issn = {1664-3224},
	year = {2024},
	eissn = {1664-3224},
	orcid-numbers = {Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:34790193,
	title = {Lectin-Based Immunophenotyping and Whole Proteomic Profiling of CT-26 Colon Carcinoma Murine Model.},
	url = {https://m2.mtmt.hu/api/publication/34790193},
	author = {Faragó, Anna and Zvara, Ágnes and Tiszlavicz, László and Hunyadi-Gulyás Éva, Csilla and Darula, Zsuzsanna and Hegedűs, Zoltán and Szabó, Enikő and Surguta, Sára Eszter and Tóvári, József and Puskás, László and Szebeni, Gábor},
	doi = {10.3390/ijms25074022},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34790193},
	issn = {1661-6596},
	abstract = {A murine colorectal carcinoma (CRC) model was established. CT26 colon carcinoma cells were injected into BALB/c mice's spleen to study the primary tumor and the mechanisms of cell spread of colon cancer to the liver. The CRC was verified by the immunohistochemistry of Pan Cytokeratin and Vimentin expression. Immunophenotyping of leukocytes isolated from CRC-bearing BALB/c mice or healthy controls, such as CD19+ B cells, CD11+ myeloid cells, and CD3+ T cells, was carried out using fluorochrome-labeled lectins. The binding of six lectins to white blood cells, such as galectin-1 (Gal1), siglec-1 (Sig1), Sambucus nigra lectin (SNA), Aleuria aurantia lectin (AAL), Phytolacca americana lectin (PWM), and galectin-3 (Gal3), was assayed. Flow cytometric analysis of the splenocytes revealed the increased binding of SNA, and AAL to CD3 + T cells and CD11b myeloid cells; and increased siglec-1 and AAL binding to CD19 B cells of the tumor-bearing mice. The whole proteomic analysis of the established CRC-bearing liver and spleen versus healthy tissues identified differentially expressed proteins, characteristic of the primary or secondary CRC tissues. KEGG Gene Ontology bioinformatic analysis delineated the established murine CRC characteristic protein interaction networks, biological pathways, and cellular processes involved in CRC. Galectin-1 and S100A4 were identified as upregulated proteins in the primary and secondary CT26 tumor tissues, and these were previously reported to contribute to the poor prognosis of CRC patients. Modelling the development of liver colonization of CRC by the injection of CT26 cells into the spleen may facilitate the understanding of carcinogenesis in human CRC and contribute to the development of novel therapeutic strategies.},
	keywords = {colorectal carcinoma; lectin binding sugar code; proteomic analysis of murine CRC},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Tóvári, József/0000-0002-5543-3204; Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:34721641,
	title = {Mutation in Arabidopsis mitochondrial Pentatricopeptide repeat 40 gene affects tolerance to water deficit},
	url = {https://m2.mtmt.hu/api/publication/34721641},
	author = {Kant, Kamal and Rigó, Gábor and Faragó, Dóra and Benyó, Dániel and Tengölics, Roland and Szabados, László and Zsigmond, Laura},
	doi = {10.1007/s00425-024-04354-w},
	journal-iso = {PLANTA},
	journal = {PLANTA},
	volume = {259},
	unique-id = {34721641},
	issn = {0032-0935},
	year = {2024},
	eissn = {1432-2048},
	orcid-numbers = {Benyó, Dániel/0000-0002-4537-2866; Zsigmond, Laura/0000-0002-1388-1762}
}

@article{MTMT:34721386,
	title = {The Proteome of Extracellular Vesicles Released from Pulmonary Microvascular Endothelium Reveals Impact of Oxygen Conditions on Biotrauma},
	url = {https://m2.mtmt.hu/api/publication/34721386},
	author = {Schaubmayr, W. and Hochreiter, B. and Hunyadi-Gulyás Éva, Csilla and Riegler, L. and Schmidt, K. and Tiboldi, A. and Moser, B. and Klein, K.U. and Krenn, K. and Scharbert, G. and Mohr, T. and Schmid, J.A. and Spittler, A. and Tretter, V.},
	doi = {10.3390/ijms25042415},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34721386},
	issn = {1661-6596},
	abstract = {The lung can experience different oxygen concentrations, low as in hypoxia, high as under supplemental oxygen therapy, or oscillating during intermittent hypoxia as in obstructive sleep apnea or intermittent hypoxia/hyperoxia due to cyclic atelectasis in the ventilated patient. This study aimed to characterize the oxygen-condition-specific protein composition of extracellular vesicles (EVs) released from human pulmonary microvascular endothelial cells in vitro to decipher their potential role in biotrauma using quantitative proteomics with bioinformatic evaluation, transmission electron microscopy, flow cytometry, and non-activated thromboelastometry (NATEM). The release of vesicles enriched in markers CD9/CD63/CD81 was enhanced under intermittent hypoxia, strong hyperoxia and intermittent hypoxia/hyperoxia. Particles with exposed phosphatidylserine were increased under intermittent hypoxia. A small portion of vesicles were tissue factor-positive, which was enhanced under intermittent hypoxia and intermittent hypoxia/hyperoxia. EVs from treatment with intermittent hypoxia induced a significant reduction of Clotting Time in NATEM analysis compared to EVs isolated after normoxic exposure, while after intermittent hypoxia/hyperoxia, tissue factor in EVs seems to be inactive. Gene set enrichment analysis of differentially expressed genes revealed that EVs from individual oxygen conditions potentially induce different biological processes such as an inflammatory response under strong hyperoxia and intermittent hypoxia/hyperoxia and enhancement of tumor invasiveness under intermittent hypoxia. © 2024 by the authors.},
	keywords = {ANGIOTENSIN-CONVERTING ENZYME; OXYGEN; TISSUE FACTOR; Extracellular vesicles; Pulmonary endothelium; vesicle proteomics},
	year = {2024},
	eissn = {1422-0067}
}

@article{MTMT:34497633,
	title = {Comparative analysis of hippocampal extracellular space uncovers widely altered peptidome upon epileptic seizure in urethane-anaesthetized rats},
	url = {https://m2.mtmt.hu/api/publication/34497633},
	author = {Tukacs, Vanda and Mittli, Dániel Árpád and Hunyadi-Gulyás Éva, Csilla and Darula, Zsuzsanna and Juhász, Gábor Dénes and Kardos, József and Kékesi, Adrienna Katalin},
	doi = {10.1186/s12987-024-00508-w},
	journal-iso = {FLUIDS BARRIERS CNS},
	journal = {FLUIDS AND BARRIERS OF THE CNS},
	volume = {21},
	unique-id = {34497633},
	issn = {2045-8118},
	abstract = {Background: The brain extracellular fluid (ECF), composed of secreted neurotransmitters, metabolites, peptides, and proteins, may reflect brain processes. Analysis of brain ECF may provide new potential markers for synaptic activity or brain damage and reveal additional information on pathological alterations. Epileptic seizure induction is an acute and harsh intervention in brain functions, and it can activate extra- and intracellular proteases, which implies an altered brain secretome. Thus, we applied a 4-aminopyridine (4-AP) epilepsy model to study the hippocampal ECF peptidome alterations upon treatment in rats. Methods: We performed in vivo microdialysis in the hippocampus for 3–3 h of control and 4-AP treatment phase in parallel with electrophysiology measurement. Then, we analyzed the microdialysate peptidome of control and treated samples from the same subject by liquid chromatography-coupled tandem mass spectrometry. We analyzed electrophysiological and peptidomic alterations upon epileptic seizure induction by two-tailed, paired t-test. Results: We detected 2540 peptides in microdialysate samples by mass spectrometry analysis; and 866 peptides—derived from 229 proteins—were found in more than half of the samples. In addition, the abundance of 322 peptides significantly altered upon epileptic seizure induction. Several proteins of significantly altered peptides are neuropeptides (Chgb) or have synapse- or brain-related functions such as the regulation of synaptic vesicle cycle (Atp6v1a, Napa), astrocyte morphology (Vim), and glutamate homeostasis (Slc3a2). Conclusions: We have detected several consequences of epileptic seizures at the peptidomic level, as altered peptide abundances of proteins that regulate epilepsy-related cellular processes. Thus, our results indicate that analyzing brain ECF by in vivo microdialysis and omics techniques is useful for monitoring brain processes, and it can be an alternative method in the discovery and analysis of CNS disease markers besides peripheral fluid analysis. © 2024, The Author(s).},
	keywords = {Animals; Male; PEPTIDES; metabolism; PEPTIDE; SEIZURES; comparative study; amino acid sequence; Extracellular Space; nonhuman; animal model; animal experiment; pathology; slow brain wave; Electroencephalogram; urethan; glial fibrillary acidic protein; area under the curve; in vivo study; seizure; AMIDES; AMIDE; upregulation; brain function; neuropeptide Y; ASTROCYTE; tandem mass spectrometry; Temporal lobe epilepsy; Urethane; MICRODIALYSIS; synapse vesicle; FAMPRIDINE; peptidomics; Gene set enrichment analysis; rat; secretoneurin; Chromogranin B},
	year = {2024},
	eissn = {2045-8118},
	orcid-numbers = {Juhász, Gábor Dénes/0000-0002-0849-6931; Kardos, József/0000-0002-2135-2932; Kékesi, Adrienna Katalin/0000-0003-3042-4878}
}

@article{MTMT:34491425,
	title = {Droplet navigation on metastable hydrophobic and superhydrophobic nonwoven materials},
	url = {https://m2.mtmt.hu/api/publication/34491425},
	author = {Sharma, Sumit and Shukla, Siddharth and Rawal, Amit and Jee, Shyam and Ayaydin, Ferhan and Vásárhelyi, Lívia and Kukovecz, Ákos and Kumar, Vijay and Kadi, Nawar},
	doi = {10.1016/j.colsurfa.2023.132993},
	journal-iso = {COLLOID SURFACE A},
	journal = {COLLOIDS AND SURFACES A : PHYSICOCHEMICAL AND ENGINEERING ASPECTS},
	volume = {683},
	unique-id = {34491425},
	issn = {0927-7757},
	year = {2024},
	eissn = {1873-4359},
	pages = {132993},
	orcid-numbers = {Sharma, Sumit/0000-0001-8851-7546; Shukla, Siddharth/0000-0002-3405-431X; Vásárhelyi, Lívia/0000-0001-8943-9500; Kukovecz, Ákos/0000-0003-0716-9557; Kadi, Nawar/0000-0002-1286-7053}
}

@article{MTMT:34479463,
	title = {The metabolic domestication syndrome of budding yeast},
	url = {https://m2.mtmt.hu/api/publication/34479463},
	author = {Tengölics, Roland and Szappanos, Balázs and Mülleder, M and Kalapis, Dorottya and Grézal, Gábor and Sajben, Cs and Agostini, F and Mokochinski, Joao Benhur and Bálint, Balázs and Nagy, LG and Ralser, M and Papp, Balázs},
	doi = {10.1073/pnas.2313354121},
	journal-iso = {P NATL ACAD SCI USA},
	journal = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
	volume = {121},
	unique-id = {34479463},
	issn = {0027-8424},
	year = {2024},
	eissn = {1091-6490},
	orcid-numbers = {Szappanos, Balázs/0000-0002-5075-1799; Grézal, Gábor/0000-0003-1685-4791}
}

@article{MTMT:34435877,
	title = {Chloroplast phosphate transporter CrPHT4-7 regulates phosphate homeostasis and photosynthesis in Chlamydomonas},
	url = {https://m2.mtmt.hu/api/publication/34435877},
	author = {Tóth, Dávid and Kuntam, Soujanya and Ferenczi, Áron and Vidal-Meireles, Andre and Kovács, László and Wang, Lianyong and Sarkadi, Zsuzsa and Migh, Ede and Szentmihályi, Klára and Tengölics, Roland and Neupert, Juliane and Bock, Ralph and Jonikas, Martin C and Molnar, Attila and Tóth, Szilvia Zita},
	doi = {10.1093/plphys/kiad607},
	journal-iso = {PLANT PHYSIOL},
	journal = {PLANT PHYSIOLOGY},
	volume = {194},
	unique-id = {34435877},
	issn = {0032-0889},
	abstract = {In eukaryotic cells, phosphorus is assimilated and utilized primarily as phosphate (Pi). Pi homeostasis is mediated by transporters that have not yet been adequately characterized in green algae. This study reports on PHOSPHATE TRANSPORTER 4-7 (CrPHT4-7) from Chlamydomonas reinhardtii, a member of the PHT4 transporter family, which exhibits remarkable similarity to AtPHT4;4 from Arabidopsis (Arabidopsis thaliana), a chloroplastic ascorbate transporter. Using fluorescent protein tagging, we show that CrPHT4-7 resides in the chloroplast envelope membrane. Crpht4-7 mutants, generated by the CRISPR/Cas12a-mediated single-strand templated repair, show retarded growth, especially in high light, reduced ATP level, strong ascorbate accumulation, and diminished non-photochemical quenching in high light. On the other hand, total cellular phosphorous content was unaffected, and the phenotype of the Crpht4-7 mutants could not be alleviated by ample Pi supply. CrPHT4-7-overexpressing lines exhibit enhanced biomass accumulation under high light conditions in comparison with the wild-type strain. Expressing CrPHT4-7 in a yeast (Saccharomyces cerevisiae) strain lacking Pi transporters substantially recovered its slow growth phenotype, demonstrating that CrPHT4-7 transports Pi. Even though CrPHT4-7 shows a high degree of similarity to AtPHT4;4, it does not display any substantial ascorbate transport activity in yeast or intact algal cells. Thus, the results demonstrate that CrPHT4-7 functions as a chloroplastic Pi transporter essential for maintaining Pi homeostasis and photosynthesis in C. reinhardtii.},
	year = {2024},
	eissn = {1532-2548},
	pages = {1646-1661},
	orcid-numbers = {Ferenczi, Áron/0000-0002-2100-1702; Wang, Lianyong/0000-0003-0906-8346; Szentmihályi, Klára/0000-0002-3618-8151; Neupert, Juliane/0000-0002-4082-9484; Bock, Ralph/0000-0001-7502-6940; Jonikas, Martin C/0000-0002-9519-6055; Molnar, Attila/0000-0002-1044-6327}
}

@article{MTMT:34486293,
	title = {Single-cell immunophenotyping revealed the association of CD4+ central and CD4+ effector memory T cells linking exacerbating chronic obstructive pulmonary disease and NSCLC.},
	url = {https://m2.mtmt.hu/api/publication/34486293},
	author = {Gémes, Nikolett and Balog, József Ágoston and Neuperger, Patricia and Schlegl, Erzsébet and Barta, Imre and Fillinger, János and Antus, Balázs and Zvara, Ágnes and Hegedűs, Zoltán and Czimmerer, Zsolt and Manczinger, Máté and Balogh, Gergő Mihály and Tóvári, József and Puskás, László and Szebeni, Gábor},
	doi = {10.3389/fimmu.2023.1297577},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {14},
	unique-id = {34486293},
	issn = {1664-3224},
	abstract = {Tobacco smoking generates airway inflammation in chronic obstructive pulmonary disease (COPD), and its involvement in the development of lung cancer is still among the leading causes of early death. Therefore, we aimed to have a better understanding of the disbalance in immunoregulation in chronic inflammatory conditions in smoker subjects with stable COPD (stCOPD), exacerbating COPD (exCOPD), or non-small cell lung cancer (NSCLC).Smoker controls without chronic illness were recruited as controls. Through extensive mapping of single cells, surface receptor quantification was achieved by single-cell mass cytometry (CyTOF) with 29 antibodies. The CyTOF characterized 14 main immune subsets such as CD4+, CD8+, CD4+/CD8+, CD4-/CD8-, and γ/δ T cells and other subsets such as CD4+ or CD8+ NKT cells, NK cells, B cells, plasmablasts, monocytes, CD11cdim, mDCs, and pDCs. The CD4+ central memory (CM) T cells (CD4+/CD45RA-/CD45RO+/CD197+) and CD4+ effector memory (EM) T cells (CD4+/CD45RA-/CD45RO+/CD197-) were FACS-sorted for RNA-Seq analysis. Plasma samples were assayed by Luminex MAGPIX® for the quantitative measurement of 17 soluble immuno-oncology mediators (BTLA, CD28, CD80, CD27, CD40, CD86, CTLA-4, GITR, GITRL, HVEM, ICOS, LAG-3, PD-1, PD-L1, PD-L2, TIM-3, TLR-2) in the four studied groups.Our focus was on T-cell-dependent differences in COPD and NSCLC, where peripheral CD4+ central memory and CD4+ effector memory cells showed a significant reduction in exCOPD and CD4+ CM showed elevation in NSCLC. The transcriptome analysis delineated a perfect correlation of differentially expressed genes between exacerbating COPD and NSCLC-derived peripheral CD4+ CM or CD4+ EM cells. The measurement of 17 immuno-oncology soluble mediators revealed a disease-associated phenotype in the peripheral blood of stCOPD, exCOPD, and NSCLC patients.The applied single-cell mass cytometry, the whole transcriptome profiling of peripheral CD4+ memory cells, and the quantification of 17 plasma mediators provided complex data that may contribute to the understanding of the disbalance in immune homeostasis generated or sustained by tobacco smoking in COPD and NSCLC.},
	keywords = {Tobacco smoking; non-small cell lung cancer; single-cell mass cytometry; CD4 central memory T cells; CD4 effector memory T cells; exacerbating COPD; stable COPD},
	year = {2023},
	eissn = {1664-3224},
	orcid-numbers = {Manczinger, Máté/0000-0003-0831-9617; Tóvári, József/0000-0002-5543-3204; Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:34446740,
	title = {Distinctive features of Zaprionus indianus hemocyte differentiation and function revealed by transcriptomic analysis},
	url = {https://m2.mtmt.hu/api/publication/34446740},
	author = {Cinege, Gyöngyi Ilona and Magyar, Lilla Brigitta and Kovács, Henrietta and Varga, Viktória and Bodai, László and Zsindely, Nóra and Nagy, Gábor and Hegedűs, Zoltán and Hultmark, Dan and Andó, István},
	doi = {10.3389/fimmu.2023.1322381},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {14},
	unique-id = {34446740},
	issn = {1664-3224},
	year = {2023},
	eissn = {1664-3224},
	orcid-numbers = {Bodai, László/0000-0001-8411-626X; Zsindely, Nóra/0000-0002-6189-3100; Nagy, Gábor/0000-0001-5464-1135; Andó, István/0000-0002-4648-9396}
}