@article{MTMT:1999468, title = {Small-angle neutron scattering study of the ultrastructure of chloroplast thylakoid membranes - Periodicity and structural flexibility of the stroma lamellae}, url = {https://m2.mtmt.hu/api/publication/1999468}, author = {Posselt, D and Nagy, Gergely and Kirkensgaard, J J K and Holm, J K and Aagaard, T H and Timmins, P and Rétfalvi, Eszter and Rosta, László and Kovács, László and Garab, Győző}, doi = {10.1016/j.bbabio.2012.01.012}, journal-iso = {BBA-BIOENERGETICS}, journal = {BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS}, volume = {1817}, unique-id = {1999468}, issn = {0005-2728}, abstract = {The multilamellar organization of freshly isolated spinach and pea chloroplast thylakoid membranes was studied using small- angle neutron scattering. A broad peak at ∼ 0.02 Å - 1 is ascribed to diffraction from domains of ordered, unappressed stroma lamellae, revealing a repeat distance of 294 Å ± 7 Å in spinach and 345 Å ± 11 Å in pea. The peak position and hence the repeat distance of stroma lamellae is strongly dependent on the osmolarity and the ionic strength of the suspension medium, as demonstrated by varying the sorbitol and the Mg ++-concentration in the sample. For pea thylakoid membranes, we show that the repeat distance decreases when illuminating the sample with white light, in accordance with our earlier results on spinach, also regarding the observation that addition of an uncoupler prohibits the light-induced structural changes, a strong indication that these changes are driven by the transmembrane proton gradient. We show that the magnitude of the shrinkage is strongly dependent on light intensity and that the repeat distance characteristic of the dark state after illumination is different from the initial dark state. Prolonged strong illumination leads to irreversible changes and swelling as reflected in increased repeat distances. The observed reorganizations are discussed within the frames of the current structural models of the granum-stroma thylakoid membrane assembly and the regulatory mechanisms in response to variations in the environmental conditions in vivo. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial. © 2011 Elsevier B.V. All rights reserved.}, year = {2012}, eissn = {1879-2650}, pages = {1220-1228} } @article{MTMT:1999479, title = {Practice of 2D data treatment in SAS. 2nd International Workshop on SANS-YuMO User Meeting at the Start-up of Scientific Experiments on the IBR-2M Reactor: Devoted to the 75th Anniversary of Yu M Ostanevich's Birth}, url = {https://m2.mtmt.hu/api/publication/1999479}, author = {Pépy, Gerard}, doi = {10.1088/1742-6596/351/1/012025}, journal-iso = {J PHYS CONF SER}, journal = {JOURNAL OF PHYSICS-CONFERENCE SERIES}, volume = {351}, unique-id = {1999479}, issn = {1742-6588}, abstract = {Many Small Angle Scattering experiments are one dimensional. Meanwhile the data of some experiments are 2 dimensional and the experimentalist may find advantages to make a full 2 dimensional data treatment or cannot avoid it. 2 dimensional data treatments present specific difficulties. The first one is an appropriate display of the whole data and of the most important part of them, in a way significant for the scientist. The second difficulty comes from the bad statistics of data which cannot be grouped together. In the following paper we describe various solutions to these problems, developed through a very long practice in LLB (Saclay, France) and SzFKI (Budapest, Hungary). © Published under licence by IOP Publishing Ltd.}, year = {2012}, eissn = {1742-6596} } @article{MTMT:1999482, title = {Ordering of hydroxylated fullerenes in aqueous solutions}, url = {https://m2.mtmt.hu/api/publication/1999482}, author = {Nikolaev, I V and Lebedev, V T and Grushko, Y S and Sedov, V P and Shilin, V A and Török, Gyula and Melenevskaya, E Y}, doi = {10.1080/1536383X.2012.655176}, journal-iso = {FULLER NANOTUB CAR N}, journal = {FULLERENES NANOTUBES AND CARBON NANOSTRUCTURES}, volume = {20}, unique-id = {1999482}, issn = {1536-383X}, abstract = {Fullerenols' C 60(OH)X self-assembly in aqueous solutions (25C, pH = 5-8) has been studied by small-angle neutron scattering. The solution of C 60(OH)X (X 20, content CF = 0.5 mg/ml, pH = 5) has shown the increase of short-range order by the addition of Na-citrates, while the Na-phosphates have induced the formation of two-level structures with tiny particles (size 6 nm) packed into large clusters (gyration radius 30 nm, fullerenols' volume fraction 50%). The observed peculiarities of fullerenes association are important for the development of their medical applications. © 2012 Copyright Taylor and Francis Group, LLC.}, year = {2012}, eissn = {1536-4046}, pages = {345-350} } @article{MTMT:1999498, title = {Diagnostic and analysis of aggregation stability of magnetic fluids for biomedical applications by small-angle neutron scattering. 4th Nanotechnology International Forum, RUSNANOTECH 2011}, url = {https://m2.mtmt.hu/api/publication/1999498}, author = {Petrenko, V I and Avdeev, M V and Bulavin, L A and Vekas, L and Rosta, László and Garamus, V M and Willumeit, R and Aksenov, V L}, doi = {10.1088/1742-6596/345/1/012028}, journal-iso = {J PHYS CONF SER}, journal = {JOURNAL OF PHYSICS-CONFERENCE SERIES}, volume = {345}, unique-id = {1999498}, issn = {1742-6588}, abstract = {Diagnostics of aggregation and determination of the aggregation regimes and their control in biocompatible magnetic fluids are necessary for their development in biomedical applications. Small-angle neutron scattering (SANS) method was applied in the structure analysis of various types of magnetic fluids for biomedical applications. Additionally the interaction characteristics between surfactant/polymer molecules used in stabilization of magnetic fluids were investigated, which is very important for understanding the synthesis procedure of highly stable magnetic fluids with controllable properties.}, year = {2012}, eissn = {1742-6596} } @article{MTMT:1999506, title = {Dynamic properties of photosystem II membranes at physiological temperatures characterized by elastic incoherent neutron scattering. Increased flexibility associated with the inactivation of the oxygen evolving complex}, url = {https://m2.mtmt.hu/api/publication/1999506}, author = {Nagy, Gergely and Pieper, J and Krumova, S B and Kovács, László and Trapp, M and Garab, Győző and Peters, J}, doi = {10.1007/s11120-011-9701-x}, journal-iso = {PHOTOSYNTH RES}, journal = {PHOTOSYNTHESIS RESEARCH}, volume = {111}, unique-id = {1999506}, issn = {0166-8595}, abstract = {Elastic incoherent neutron scattering (EINS), a non-invasive technique which is capable of measuring the mean square displacement of atoms in the sample, has been widely used in biology for exploring the dynamics of proteins and lipid membranes but studies on photosynthetic systems are scarce. In this study we investigated the dynamic characteristics of Photosystem II (PSII) membrane fragments between 280 and 340 K, i.e., in the physiological temperature range and in the range of thermal denaturation of some of the protein complexes. The mean square displacement values revealed the presence of a hydration-sensitive transition in the sample between 310 and 320 K, suggesting that the oxygen evolving complex (OEC) plays an important role in the transition. Indeed, in samples in which the OEC had been removed by TRIS- or heat-treatments (323 and 333 K) no such transition was found. Further support on the main role of OEC in these reorganizations is provided by data obtained from differential scanning calorimetry experiments, showing marked differences between the untreated and TRIS-treated samples. In contrast, circular dichroism spectra exhibited only minor changes in the excitonic interactions below 323 K, showing that the molecular organization of the pigment-protein complexes remains essentially unaffected. Our data, along with earlier incoherent neutron scattering data on PSII membranes at cryogenic temperatures (Pieper et al., Biochemistry 46:11398-11409, 2007), demonstrate that this technique can be applied to characterize the dynamic features of PSII membranes, and can be used to investigate photosynthetic membranes under physiologically relevant experimental conditions. © 2011 Springer Science+Business Media B.V.}, year = {2012}, eissn = {1573-5079}, pages = {113-124} } @article{MTMT:1999520, title = {How Can Neutrons Contribute to Cultural Heritage Research?}, url = {https://m2.mtmt.hu/api/publication/1999520}, author = {Kasztovszky, Zsolt and Rosta, László}, doi = {10.1080/10448632.2012.645689}, journal-iso = {NEUTRON NEWS}, journal = {NEUTRON NEWS}, volume = {23}, unique-id = {1999520}, issn = {1044-8632}, year = {2012}, eissn = {1931-7352}, pages = {25-28} } @article{MTMT:1999521, title = {Neutron Holography - a Brief History and Overview}, url = {https://m2.mtmt.hu/api/publication/1999521}, author = {Cser, László and Krexner, G and Markó, Márton and Szakál, Alex}, doi = {10.1080/10448632.2012.645691}, journal-iso = {NEUTRON NEWS}, journal = {NEUTRON NEWS}, volume = {23}, unique-id = {1999521}, issn = {1044-8632}, year = {2012}, eissn = {1931-7352}, pages = {17-20} } @article{MTMT:1999522, title = {Nano-scale Morphology of Inclusions in Tungsten Wires - a Complex SANS Study}, url = {https://m2.mtmt.hu/api/publication/1999522}, author = {Rosta, László and Len, Adél and Pépy, Gerard and Harmat, Péter}, doi = {10.1080/10448632.2012.645697}, journal-iso = {NEUTRON NEWS}, journal = {NEUTRON NEWS}, volume = {23}, unique-id = {1999522}, issn = {1044-8632}, year = {2012}, eissn = {1931-7352}, pages = {13-16} } @article{MTMT:1999968, title = {First implementation of novel multiplexing techniques for advanced instruments at pulsed neutron sources. 5th European Conference on Neutron Scattering, ECNS 2011}, url = {https://m2.mtmt.hu/api/publication/1999968}, author = {Russina, M and Mezei, Ferenc and Káli, György}, doi = {10.1088/1742-6596/340/1/012018}, journal-iso = {J PHYS CONF SER}, journal = {JOURNAL OF PHYSICS-CONFERENCE SERIES}, volume = {340}, unique-id = {1999968}, issn = {1742-6588}, abstract = {Novel multiplexing techniques, such as Repetition Rate Multiplication and Wavelength Frame Multiplication imply the use of a set of monochromatic wavelengths or a set of wavelength bands coming from the same source pulse by using novel combinations of standard mechanical neutron choppers. In this case the instrumental parameters, such as wavelength resolution, wavelength band, repetition rate are not any more determined by the source parameters, but can be flexibly defined by the chopper frequencies, speeds and slits. Here we report about the first experimental implementation of Repetition Rate Multiplication (RRM) and Wavelength Frame Multiplication (WFM). For this purpose the TOF spectrometer NEAT at HZB, Berlin and TOF diffractometer at BNC, Budapest have been used in non-standard modes of operation. Our results provide full proof-of-principle of the RRM and WFM methods and clearly show the extensive capability of these methods to achieve multiply enhanced data collection rates by individually tuning for each experiment the pulse length and/or pulse repetition rate within broad limits and independently from the actual source pulse parameters.}, year = {2012}, eissn = {1742-6596} } @article{MTMT:1999973, title = {Time-of-Flight Neutron Diffraction for Long Pulse Neutron Sources}, url = {https://m2.mtmt.hu/api/publication/1999973}, author = {Mezei, Ferenc and Russina, M and Káli, György}, doi = {10.1080/10448632.2012.645686}, journal-iso = {NEUTRON NEWS}, journal = {NEUTRON NEWS}, volume = {23}, unique-id = {1999973}, issn = {1044-8632}, year = {2012}, eissn = {1931-7352}, pages = {29-31} }