TY - CONF AU - Nochta-Kazsoki, Adrienn Katalin AU - Abboud, Houssam AU - Niczinger, Noémi AU - Nándor, Nagy AU - Zelkó, Romána TI - Development and characterization of a Nanofibrous Ophthalmic Insert for the Treatment of Age-related Macular Degeneration T2 - PhD Scientific Days 2024 PY - 2024 UR - https://m2.mtmt.hu/api/publication/35314817 ID - 35314817 LA - English DB - MTMT ER - TY - JOUR AU - Farkas, Eszter AU - Abboud, Houssam AU - Nagy, Nándor AU - Hofmeister, Bálint AU - Ostorházi, Eszter AU - Tóth, Bence AU - Pinke, Balázs AU - Mészáros, László AU - Zelkó, Romána AU - Nochta-Kazsoki, Adrienn Katalin TI - Formulation and Development of Nanofiber-Based Ophthalmic Insert for the Treatment of Bacterial Conjunctivitis JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 17 PG - 22 SN - 1661-6596 DO - 10.3390/ijms25179228 UR - https://m2.mtmt.hu/api/publication/35181230 ID - 35181230 LA - English DB - MTMT ER - TY - JOUR AU - Vilimi, Zsófia AU - Pápay, Zsófia Edit AU - Basa, Bálint AU - Orekhova, Xeniya AU - Kállai-Szabó, Nikolett AU - Antal, István TI - Microfluidic Rheology: An Innovative Method for Viscosity Measurement of Gels and Various Pharmaceuticals JF - GELS (BASEL) J2 - GELS-BASEL VL - 10 PY - 2024 IS - 7 PG - 14 SN - 2310-2861 DO - 10.3390/gels10070464 UR - https://m2.mtmt.hu/api/publication/35149948 ID - 35149948 AB - Measuring the viscosity of pharmaceutical dosage forms is a crucial process. Viscosity provides information about the stability of the composition, the release rate of the drug, bioavailability, and, in the case of injectable drug formulations, even the force required for injection. However, measuring viscosity is a complex task with numerous challenges, especially for non-Newtonian materials, which include most pharmaceutical formulations, such as gels. Selecting the appropriate shear rate is critical. Since viscosity in many systems is highly temperature-dependent, stable temperature control is necessary during the measurement. Using microfluidics technology, it is now possible to perform rheological characterization and conduct fast and accurate measurements. Small sample volumes (even below 500 µL) are required, and viscosity determination can be carried out over a wide range of shear rates. Nevertheless, the pharmaceutical application of viscometers operating on the principle of microfluidics is not yet widespread. In our work, we compare the results of measurements taken with a microfluidic chip-based viscometer on different pharmaceutical forms (gels, solution) with those obtained using a traditional rotational viscometer, evaluating the relative advantages and disadvantages of the different methods. The microfluidics-based method enables time- and sample-efficient viscosity analysis of the examined pharmaceutical forms. LA - English DB - MTMT ER - TY - JOUR AU - Kocsis, Dorottya AU - Dhinakaran, Shanmugam AU - Pandey, Divyam AU - Laki, András József AU - Laki, Mária AU - Sztankovics, Dániel AU - Lengyel, Miléna AU - Vrábel, Judit AU - Naszlady, Márton Bese AU - Sebestyén, Anna AU - Ponmozhi, Jeyaraj AU - Antal, István AU - Erdő, Franciska TI - Fluid Dynamics Optimization of Microfluidic Diffusion Systems for Assessment of Transdermal Drug Delivery: An Experimental and Simulation Study JF - SCIENTIA PHARMACEUTICA J2 - SCI PHARM VL - 92 PY - 2024 IS - 2 PG - 20 SN - 0036-8709 DO - 10.3390/scipharm92020035 UR - https://m2.mtmt.hu/api/publication/35062202 ID - 35062202 N1 - Funding Agency and Grant Number: Ministry of Innovation and Technology, Hungary [TKP2021-EGA-42]; National Research Development and Innovation Fund; New National Excellence Program of the Ministry for Culture and Innovation from the National Research, Development, and Innovation Fund, Hungary [UNKP-23-3]; Science and Engineering Research Board (SERB), Government of India [CRG/2021/008464] Funding text: This work was supported by the TKP2021-EGA-42 grant, funded by the Ministry of Innovation and Technology, Hungary, with support from the National Research Development and Innovation Fund under the TKP2021 program and the UNKP-23-3 New National Excellence Program of the Ministry for Culture and Innovation from the National Research, Development, and Innovation Fund, Hungary. The authors Shanmugam Dhinakaran and Jeyaraj Ponmozhi acknowledge fundingreceived from the Science and Engineering Research Board (SERB), Government of India, under grantno. CRG/2021/008464. AB - Organ-on-a-chip technologies show exponential growth driven by the need to reduce the number of experimental animals and develop physiologically relevant human models for testing drugs. In vitro, microfluidic devices should be carefully designed and fabricated to provide reliable tools for modeling physiological or pathological conditions and assessing, for example, drug delivery through biological barriers. The aim of the current study was to optimize the utilization of three existing skin-on-a-chip microfluidic diffusion chambers with various designs. For this, different perfusion flow rates were compared using cellulose acetate membrane, polyester membrane, excised rat skin, and acellular alginate scaffold in the chips. These diffusion platforms were integrated into a single-channel microfluidic diffusion chamber, a multi-channel chamber, and the LiveBox2 system. The experimental results revealed that the 40 µL/min flow rate resulted in the highest diffusion of the hydrophilic model formulation (2% caffeine cream) in each system. The single-channel setup was used for further analysis by computational fluid dynamics simulation. The visualization of shear stress and fluid velocity within the microchannel and the presentation of caffeine progression with the perfusion fluid were consistent with the measured data. These findings contribute to the development and effective application of microfluidic systems for penetration testing. LA - English DB - MTMT ER - TY - JOUR AU - Császár-Nagy, Noémi AU - Bob, Petr AU - Bókkon, István TI - Long-Term Implicit Epigenetic Stress Information in the Enteric Nervous System and its Contribution to Developing and Perpetuating IBS JF - CURRENT NEUROPHARMACOLOGY J2 - CURR NEUROPHARMACOL VL - 22 PY - 2024 IS - 13 SP - 2100 EP - 2112 PG - 13 SN - 1570-159X DO - 10.2174/1570159X22666240507095700 UR - https://m2.mtmt.hu/api/publication/34871984 ID - 34871984 AB - Psychiatric and mood disorders may play an important role in the development and persistence of irritable bowel syndrome (IBS). Previously, we hypothesized that stress-induced implicit memories may persist throughout life via epigenetic processes in the enteric nervous system (ENS), independent of the central nervous system (CNS). These epigenetic memories in the ENS may contribute to developing and perpetuating IBS. Here, we further elaborate on our earlier hypothesis. That is, during pregnancy, maternal prenatal stresses perturb the HPA axis and increase circulating cortisol levels, which can affect the maternal gut microbiota. Maternal cortisol can cross the placental barrier and increase cortisol-circulating levels in the fetus. This leads to dysregulation of the HPA axis, affecting the gut microbiota, microbial metabolites, and intestinal permeability in the fetus. Microbial metabolites, such as short-chain fatty acids (which also regulate the development of fetal ENS), can modulate a range of diseases by inducing epigenetic changes. These mentioned processes suggest that stress-related, implicit, long-term epigenetic memories may be programmed into the fetal ENS during pregnancy. Subsequently, this implicit epigenetic stress information from the fetal ENS could be conveyed to the CNS through the bidirectional microbiota-gut-brain axis (MGBA), leading to perturbed functional connectivity among various brain networks and the dysregulation of affective and pain processes. LA - English DB - MTMT ER - TY - JOUR AU - Virág, Dávid AU - Schlosser, Gitta (Vácziné) AU - Borbély, Adina Noémi AU - Gellén, Gabriella AU - Papp, Dávid AU - Kaleta, Zoltán AU - Dalmadiné Kiss, Borbála AU - Antal, István AU - Ludányi, Krisztina TI - A Mass Spectrometry Strategy for Protein Quantification Based on the Differential Alkylation of Cysteines Using Iodoacetamide and Acrylamide JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 9 PG - 12 SN - 1661-6596 DO - 10.3390/ijms25094656 UR - https://m2.mtmt.hu/api/publication/34821651 ID - 34821651 AB - Mass spectrometry has become the most prominent yet evolving technology in quantitative proteomics. Today, a number of label-free and label-based approaches are available for the relative and absolute quantification of proteins and peptides. However, the label-based methods rely solely on the employment of stable isotopes, which are expensive and often limited in availability. Here we propose a label-based quantification strategy, where the mass difference is identified by the differential alkylation of cysteines using iodoacetamide and acrylamide. The alkylation reactions were performed under identical experimental conditions; therefore, the method can be easily integrated into standard proteomic workflows. Using high-resolution mass spectrometry, the feasibility of this approach was assessed with a set of tryptic peptides of human serum albumin. Several critical questions, such as the efficiency of labeling and the effect of the differential alkylation on the peptide retention and fragmentation, were addressed. The concentration of the quality control samples calculated against the calibration curves were within the ±20% acceptance range. It was also demonstrated that heavy labeled peptides exhibit a similar extraction recovery and matrix effect to light ones. Consequently, the approach presented here may be a viable and cost-effective alternative of stable isotope labeling strategies for the quantification of cysteine-containing proteins. LA - English DB - MTMT ER - TY - JOUR AU - Szederkényi, Gábor AU - Kocsis, Dorottya AU - Vághy, Mihály András AU - Czárán, Domonkos Tamás AU - Sasvári, Péter AU - Lengyel, Miléna AU - Naszlady, Márton Bese AU - Kreis, F AU - Antal, István AU - Csépányi-Kömi, Roland AU - Erdő, Franciska TI - Mathematical modeling of transdermal delivery of topical drug formulations in a dynamic microfluidic diffusion chamber in health and disease JF - PLOS ONE J2 - PLOS ONE VL - 19 PY - 2024 IS - 4 PG - 17 SN - 1932-6203 DO - 10.1371/journal.pone.0299501 UR - https://m2.mtmt.hu/api/publication/34813473 ID - 34813473 AB - Mathematical models of epidermal and dermal transport are essential for optimization and development of products for percutaneous delivery both for local and systemic indication and for evaluation of dermal exposure to chemicals for assessing their toxicity. These models often help directly by providing information on the rate of drug penetration through the skin and thus on the dermal or systemic concentration of drugs which is the base of their pharmacological effect. The simulations are also helpful in analyzing experimental data, reducing the number of experiments and translating the in vitro investigations to an in-vivo setting. In this study skin penetration of topically administered caffeine cream was investigated in a skin-on-a-chip microfluidic diffusion chamber at room temperature and at 32̊C. Also the transdermal penetration of caffeine in healthy and diseased conditions was compared in mouse skins from intact, psoriatic and allergic animals. In the last experimental setup dexamethasone, indomethacin, piroxicam and diclofenac were examined as a cream formulation for absorption across the dermal barrier. All the measured data were used for making mathematical simulation in a three-compartmental model. The calculated and measured results showed a good match, which findings indicate that our mathematical model might be applied for prediction of drug delivery through the skin under different circumstances and for various drugs in the novel, miniaturized diffusion chamber. © 2024 Szederkényi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, LA - English DB - MTMT ER - TY - JOUR AU - Kirschfink, M. AU - Frazer-Abel, A. AU - Bertalanné Balogh, Emese AU - Goseberg, S. AU - Weiss, N. AU - Prohászka, Zoltán TI - External quality assurance program for diagnostic complement laboratories: evaluation of the results of the past seven years JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 15 PY - 2024 PG - 18 SN - 1664-3224 DO - 10.3389/fimmu.2024.1368399 UR - https://m2.mtmt.hu/api/publication/34796435 ID - 34796435 N1 - Institute of Immunology, University of Heidelberg, Heidelberg, Germany Instand eV, Düsseldorf, Germany ExseraBioLabs, University of Colorado, Aurora, CO, United States Department of Pharmaceutics, Semmelweis University, Budapest, Hungary Department of Internal Medicine and Hematology, Füst György Complement Diagnostic Laboratory, Semmelweis University, Budapest, Hungary Export Date: 11 September 2024 Correspondence Address: Prohászka, Z.; Instand eVGermany; email: prohaszka.zoltan@semmelweis.hu LA - English DB - MTMT ER - TY - JOUR AU - Kállai-Szabó, Nikolett AU - Farkas, Dóra AU - Lengyel, Miléna AU - Basa, Bálint AU - Fleck, C. AU - Antal, István TI - Microparticles and multi-unit systems for advanced drug delivery JF - EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES J2 - EUR J PHARM SCI VL - 194 PY - 2024 PG - 25 SN - 0928-0987 DO - 10.1016/j.ejps.2024.106704 UR - https://m2.mtmt.hu/api/publication/34631071 ID - 34631071 LA - English DB - MTMT ER - TY - JOUR AU - Budavári, Bálint Péter AU - Karancsi, Áron AU - Pinke, Balázs Gábor AU - Pállinger, Éva AU - Juriga-Tóth, Krisztina AU - Király, Márton AU - Szász, Adrienn Zsófia AU - Voszka, István AU - Molnár, Kolos AU - Kőhidai, László AU - Jedlovszky-Hajdú, Angéla AU - S. Nagy, Krisztina TI - Long-term shelf-life liposomes for delivery of prednisolone and budesonide JF - JOURNAL OF MOLECULAR LIQUIDS J2 - J MOL LIQ VL - 394 PY - 2024 PG - 13 SN - 0167-7322 DO - 10.1016/j.molliq.2023.123756 UR - https://m2.mtmt.hu/api/publication/34506340 ID - 34506340 N1 - Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University, Nagyvárad tér 4., Budapest, H-1089, Hungary Department of Polymer Engineering, Faculty of Mechanical Engineering, Budapest University of Technology and Economics, Műegyetem rkp. 3-9., Budapest, H-1111, Hungary Department of Genetics, Cell- and Immunobiology, Faculty of Medicine, Semmelweis University, Nagyvárad tér 4., Budapest, H-1089, Hungary Department of Pharmaceutics, Faculty of Pharmacy, Semmelweis University, Hőgyes Endre u. 7., Budapest, H-1092, Hungary Department of Biophysics and Radiation Biology, Faculty of Medicine, Semmelweis University, Tűzoltó u. 37-47., Budapest, H-1094, Hungary ELKH–BME Research Group for Composite Science and Technology, Műegyetem rkp. 3., Budapest, H-1111, Hungary MTA-BME Lendület Sustainable Polymers Research Group, Műegyetem rkp. 3, Budapest, H-1111, Hungary Export Date: 14 March 2024 CODEN: JMLID Correspondence Address: Jedlovszky-Hajdu, A.; Laboratory of Nanochemistry, Nagyvárad tér 4., Hungary; email: hajdu.angela@med.semmelweis-univ.hu LA - English DB - MTMT ER -