@article{MTMT:34824919, title = {Anti-Nociceptive Effects of Sphingomyelinase and Methyl-Beta-Cyclodextrin in the Icilin-Induced Mouse Pain Model}, url = {https://m2.mtmt.hu/api/publication/34824919}, author = {Horváth, Ádám and Steib, Anita and Nehr-Majoros, Andrea Kinga and Kántás, Boglárka and Király, Ágnes and Racskó, Márk and Tóth, Balázs István and Szánti-Pintér, Eszter and Kudová, Eva and Skodáné Földes, Rita and Helyes, Zsuzsanna and Szőke, Éva}, doi = {10.3390/ijms25094637}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {25}, unique-id = {34824919}, issn = {1661-6596}, abstract = {The thermo- and pain-sensitive Transient Receptor Potential Melastatin 3 and 8 (TRPM3 and TRPM8) ion channels are functionally associated in the lipid rafts of the plasma membrane. We have already described that cholesterol and sphingomyelin depletion, or inhibition of sphingolipid biosynthesis decreased the TRPM8 but not the TRPM3 channel opening on cultured sensory neurons. We aimed to test the effects of lipid raft disruptors on channel activation on TRPM3- and TRPM8-expressing HEK293T cells in vitro, as well as their potential analgesic actions in TRPM3 and TRPM8 channel activation involving acute pain models in mice. CHO cell viability was examined after lipid raft disruptor treatments and their effects on channel activation on channel expressing HEK293T cells by measurement of cytoplasmic Ca2+ concentration were monitored. The effects of treatments were investigated in Pregnenolone-Sulphate-CIM-0216-evoked and icilin-induced acute nocifensive pain models in mice. Cholesterol depletion decreased CHO cell viability. Sphingomyelinase and methyl-beta-cyclodextrin reduced the duration of icilin-evoked nocifensive behavior, while lipid raft disruptors did not inhibit the activity of recombinant TRPM3 and TRPM8. We conclude that depletion of sphingomyelin or cholesterol from rafts can modulate the function of native TRPM8 receptors. Furthermore, sphingolipid cleavage provided superiority over cholesterol depletion, and this method can open novel possibilities in the management of different pain conditions.}, keywords = {PAIN; cholesterol; Sphingomyelinase; lipid raft; methyl-beta-cyclodextrin; transient receptor potential}, year = {2024}, eissn = {1422-0067}, orcid-numbers = {Szánti-Pintér, Eszter/0000-0001-8263-9884; Skodáné Földes, Rita/0000-0002-9810-1509} } @article{MTMT:34796888, title = {Decidual γδT cells of early human pregnancy produce angiogenic and immunomodulatory proteins while also possessing cytotoxic potential}, url = {https://m2.mtmt.hu/api/publication/34796888}, author = {Nörenberg, Jasper Maximilian and Vida, P. and Bösmeier, I. and Forró, B. and Nörenberg, A. and Buda, Á. and Simon, Diána and Erdő-Bonyár, Szabina and Jáksó, Pál and Kovács, Kálmán András and Mikó, Éva and Berki, Tímea and Mezősi, Emese and Barakonyi, Alíz}, doi = {10.3389/fimmu.2024.1382424}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {15}, unique-id = {34796888}, issn = {1664-3224}, year = {2024}, eissn = {1664-3224}, orcid-numbers = {Berki, Tímea/0000-0002-0134-8127; Mezősi, Emese/0000-0001-9367-3877} } @article{MTMT:34786848, title = {Translation and validation of the Hungarian version of the Birth Satisfaction Scale-Revised (BSS-R).}, url = {https://m2.mtmt.hu/api/publication/34786848}, author = {Abrán, Hunor and Kovács, Kálmán András and Horvát, Zalán and Erőss, Edina and Hollins Martin, Caroline J and Martin, Colin R}, doi = {10.1016/j.midw.2024.103983}, journal-iso = {MIDWIFERY}, journal = {MIDWIFERY}, volume = {132}, unique-id = {34786848}, issn = {0266-6138}, abstract = {Evidence relating maternal birth experience to a range of maternal and neonatal outcomes is increasingly compelling. Consequently valid and reliable self-report of birth experience from the mothers perspective is critical.The current study sought to translate and validate a Hungarian-language version of the Birth Satisfaction Scale-Revised (BSS-R).Following forward and backwards translation into Hungarian, the Hungarian BSS-R (HU-BSS-R) was administered to women in a major Transylvanian hospital maternity unit within 72 h postpartum. Key psychometric characteristics were then examined in relation to factor structure, divergent and convergent validity, internal consistency, and known-groups discriminant validity.Two-hundred and thirty-two women completed the HU-BSS-R. Confirmatory factor analysis revealed the HU-BSS-R to offer an excellent fit to data for the established tri-dimensional measurement model. The HU-BSS-R was also found to offer excellent convergent and divergent validity and known-groups discriminant validity. No significant differences were observed between internal consistency observations between the current study and the original UK validation study.The HU-BSS-R is a valid and reliable translation of the original BSS-R, it has proved itself to have excellent psychometric properties and is suitable for use in the Hungarian maternity context.}, keywords = {Psychometrics; SCALES; birth experience; birth satisfaction}, year = {2024}, eissn = {1532-3099}, orcid-numbers = {Abrán, Hunor/0000-0003-4795-9705} } @article{MTMT:34769257, title = {Reducing radicality in fertility-sparing surgery is associated with improved in vitro fertilization outcome in early-stage cervical cancer: A national retrospective study}, url = {https://m2.mtmt.hu/api/publication/34769257}, author = {Vesztergom, Dóra and Téglás, Gyöngyvér and Bahrehmand, Kiarash and Török, Attila and Balla, Levente and Forgács, Vince and Konc, János and Tándor, Zoltán and Várnagy, Ákos and Boga, Péter and Zádori, János and Sipos, Miklós and Mánfai, Zoltán and Novák, Zoltán}, doi = {10.1016/j.ygyno.2024.03.024}, journal-iso = {GYNECOL ONCOL}, journal = {GYNECOLOGIC ONCOLOGY}, volume = {186}, unique-id = {34769257}, issn = {0090-8258}, year = {2024}, eissn = {1095-6859}, pages = {35-41}, orcid-numbers = {Bahrehmand, Kiarash/0000-0002-8909-1599; Novák, Zoltán/0000-0002-2060-6654} } @article{MTMT:34763514, title = {Clinical Benefits of Decreased Photo-Oxidative Stress on Human Embryo Development}, url = {https://m2.mtmt.hu/api/publication/34763514}, author = {Gödöny, Krisztina and Herczeg, Róbert and Gyenesei, Attila and Várnagy, Ákos and Bognár, Zoltán and Kovács, L. Gábor and Szekeres, Júlia and Mauchart, Péter and Nagy, Bernadett and Erostyák, János and Kovács, Kálmán András and Bódis, József}, doi = {10.1159/000536358}, journal-iso = {MED PRIN PRACT}, journal = {MEDICAL PRINCIPLES AND PRACTICE}, unique-id = {34763514}, issn = {1011-7571}, abstract = {Objective: Early embryonic development is characterized by rapid cell division and gene activation, making the embryo extremely sensitive to environmental influences. Light exposure can affect embryonic development through a direct toxic effect on the embryo via the generation of reactive oxygen species. In a previous study, we demonstrated the positive effect of improved light-protected embryo culture conditions implemented in our laboratory. This study aimed to investigate the changes in human embryo development under light protection during the conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Materials and Methods: We tested the potential beneficial effect of light filters to reduce the risk of toxic effects of light. IVF outcomes were compared between two experimental conditions, light protection with red light filters versus no light protection as a control. Results: Blastocyst development rate in IVF was significantly higher in the light-protected group than in the group treated under conventional conditions (46.6 vs. 26.7%). In the case of ICSI, we obtained a similar result (44.5 vs. 31.6%). The rate of cryopreservation with at least one embryo was higher in the light-protected phase (32.8%) than in the conventionally manipulated phase (26.8%). The abortion rate was also significantly lower during the light-protected period in IVF, resulting in a higher live birth rate. Conclusions: The implementation of light protection to reduce the embryotoxic wavelengths of light in IVF centers may improve the blastocyst development rate and embryo quality while maintaining embryo safety.}, keywords = {blastocyst; abortion; intracytoplasmic sperm injection; In vitro fertilization; Live birth rate; light protection}, year = {2024}, eissn = {1423-0151}, orcid-numbers = {Herczeg, Róbert/0000-0002-5903-0082; Kovács, L. Gábor/0000-0001-5298-5401} } @article{MTMT:34729560, title = {Dietary nutrients during gestation cause obesity and related metabolic changes by altering DNA methylation in the offspring}, url = {https://m2.mtmt.hu/api/publication/34729560}, author = {Bokor, Szilvia and Csölle, Ildikó and Heidinger-Felső, Regina and Vass, Réka Anna and Funke Kiss, Simone and Ertl, Tibor and Molnár, Dénes}, doi = {10.3389/fendo.2024.1287255}, journal-iso = {FRONT ENDOCRINOL}, journal = {FRONTIERS IN ENDOCRINOLOGY}, volume = {15}, unique-id = {34729560}, issn = {1664-2392}, abstract = {Growing evidence shows that maternal nutrition from preconception until lactation has an important effect on the development of non-communicable diseases in the offspring. Biological responses to environmental stress during pregnancy, including undernutrition or overnutrition of various nutrients, are transmitted in part by DNA methylation. The aim of the present narrative review is to summarize literature data on altered DNA methylation patterns caused by maternal macronutrient or vitamin intake and its association with offspring's phenotype (obesity and related metabolic changes). With our literature search, we found evidence for the association between alterations in DNA methylation pattern of different genes caused by maternal under- or overnutrition of several nutrients (protein, fructose, fat, vitamin D, methyl-group donor nutrients) during 3 critical periods of programming (preconception, pregnancy, lactation) and the development of obesity or related metabolic changes (glucose, insulin, lipid, leptin, adiponectin levels, blood pressure, non-alcoholic fatty liver disease) in offspring. The review highlights that maternal consumption of several nutrients could individually affect the development of offspring's obesity and related metabolic changes via alterations in DNA methylation.}, keywords = {OBESITY; NUTRITION; metabolic syndrome; DNA methylation; epigenetic; Maternal}, year = {2024}, eissn = {1664-2392}, orcid-numbers = {Vass, Réka Anna/0000-0002-0436-4261; Molnár, Dénes/0000-0002-3675-7019} } @{MTMT:34688851, title = {Necrotizáló enterocolitis és rövidbél-szindróma}, url = {https://m2.mtmt.hu/api/publication/34688851}, author = {Ertl, Tibor}, booktitle = {Klinikai gyermekgyógyászat}, unique-id = {34688851}, year = {2024}, pages = {210-212} } @article{MTMT:34684525, title = {Integrative Epigenetic and Molecular Analysis Reveals a Novel Promoter for a New Isoform of the Transcription Factor TEAD4}, url = {https://m2.mtmt.hu/api/publication/34684525}, author = {Rashidiani, Shima and Mamo, Gizaw and Farkas, Benjámin and Szabadi, András and Farkas, Bálint and Uszkai, Veronika and Császár, András and Brandt, Barbara and Kovács, Kálmán András and Pap, Marianna and Rauch, Tibor}, doi = {10.3390/ijms25042223}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {25}, unique-id = {34684525}, issn = {1661-6596}, abstract = {TEAD4 is a transcription factor that plays a crucial role in the Hippo pathway by regulating the expression of genes related to proliferation and apoptosis. It is also involved in the maintenance and differentiation of the trophectoderm during pre- and post-implantation embryonic development. An alternative promoter for the TEAD4 gene was identified through epigenetic profile analysis, and a new transcript from the intronic region of TEAD4 was discovered using the 5'RACE method. The transcript of the novel promoter encodes a TEAD4 isoform (TEAD4-ΔN) that lacks the DNA-binding domain but retains the C-terminal protein-protein interaction domain. Gene expression studies, including end-point PCR and Western blotting, showed that full-length TEAD4 was present in all investigated tissues. However, TEAD4-ΔN was only detectable in certain cell types. The TEAD4-ΔN promoter is conserved throughout evolution and demonstrates transcriptional activity in transient-expression experiments. Our study reveals that TEAD4 interacts with the alternative promoter and increases the expression of the truncated isoform. DNA methylation plays a crucial function in the restricted expression of the TEAD4-ΔN isoform in specific tissues, including the umbilical cord and the placenta. The data presented indicate that the DNA-methylation status of the TEAD4-ΔN promoter plays a critical role in regulating organ size, cancer development, and placenta differentiation.}, keywords = {DNA methylation; TRANSCRIPTIONAL REGULATION; TEAD4; alternative promoter; Hippo/TEAD signaling}, year = {2024}, eissn = {1422-0067}, orcid-numbers = {Farkas, Bálint/0000-0003-0638-2057} } @CONFERENCE{MTMT:34667323, title = {Magyarországi területi egyenlőtlenségek elemzése a női infertilitás diagnózis esetében}, url = {https://m2.mtmt.hu/api/publication/34667323}, author = {Pónusz-Kovács, Dalma and Csákvári, Tímea and Kajos, Luca Fanni and Elmer, Diána and Pónusz, Róbert and Bódis, József and Boncz, Imre}, booktitle = {Jubileumi Szakkollégiumi Konferencia: Absztrakt kötet}, unique-id = {34667323}, year = {2024}, pages = {20-20}, orcid-numbers = {Csákvári, Tímea/0000-0002-3339-4953; Kajos, Luca Fanni/0000-0002-0574-5787; Boncz, Imre/0000-0003-3699-6236} } @article{MTMT:34641519, title = {Single-incision vaginal mesh insertion for recurrent vaginal vault prolapse after radical cystectomy and radical hysterectomy with irradiation: A case report}, url = {https://m2.mtmt.hu/api/publication/34641519}, author = {Nemeth, Zoltan and Schmidt, Roxana and Farkas, Bálint}, doi = {10.1016/j.contre.2024.100047}, journal-iso = {Continence Reports}, journal = {Continence Reports}, volume = {9}, unique-id = {34641519}, issn = {2772-9745}, year = {2024}, orcid-numbers = {Farkas, Bálint/0000-0003-0638-2057} }