TY - CHAP AU - Várnagy, Erzsébet AU - Tóth, Gergő AU - Hosztafi, Sándor AU - Fejős, Ida AU - Malanga, Milo AU - Béni, Szabolcs TI - Ciklodextrin-segített kapilláris elektroforézis: benzil-izokinolin alkaloidok enantiomer-elválasztása T2 - Biotechnológus Napok 2024 CY - Budapest SN - 9786150200880 PY - 2024 SP - 22 UR - https://m2.mtmt.hu/api/publication/34831171 ID - 34831171 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Benkő, Beáta Mária AU - Tóth, Gergő AU - Moldvai, Dorottya AU - Kádár, Szabina AU - Szabó, Edina AU - Szabó, Zoltán-István AU - Mazákné Kraszni, Márta AU - Szente, Lajos AU - Fiser, Béla AU - Sebestyén, Anna AU - Zelkó, Romána AU - Sebe, István TI - Cyclodextrin encapsulation enabling the anticancer repositioning of disulfiram: Preparation, analytical and in vitro biological characterization of the inclusion complexes JF - INTERNATIONAL JOURNAL OF PHARMACEUTICS J2 - INT J PHARM VL - In press PY - 2024 SN - 0378-5173 DO - 10.1016/j.ijpharm.2024.124187 UR - https://m2.mtmt.hu/api/publication/34830527 ID - 34830527 LA - English DB - MTMT ER - TY - JOUR AU - Virág, Dávid AU - Schlosser, Gitta AU - Borbély, Adina AU - Gellén, Gabriella AU - Papp, Dávid AU - Kaleta, Zoltán AU - Dalmadiné Kiss, Borbála AU - Antal, István AU - Ludányi, Krisztina TI - A Mass Spectrometry Strategy for Protein Quantification Based on the Differential Alkylation of Cysteines Using Iodoacetamide and Acrylamide JF - INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES J2 - INT J MOL SCI VL - 25 PY - 2024 IS - 9 SP - 4656 PG - 12 SN - 1661-6596 DO - 10.3390/ijms25094656 UR - https://m2.mtmt.hu/api/publication/34821651 ID - 34821651 AB - Mass spectrometry has become the most prominent yet evolving technology in quantitative proteomics. Today, a number of label-free and label-based approaches are available for the relative and absolute quantification of proteins and peptides. However, the label-based methods rely solely on the employment of stable isotopes, which are expensive and often limited in availability. Here we propose a label-based quantification strategy, where the mass difference is identified by the differential alkylation of cysteines using iodoacetamide and acrylamide. The alkylation reactions were performed under identical experimental conditions; therefore, the method can be easily integrated into standard proteomic workflows. Using high-resolution mass spectrometry, the feasibility of this approach was assessed with a set of tryptic peptides of human serum albumin. Several critical questions, such as the efficiency of labeling and the effect of the differential alkylation on the peptide retention and fragmentation, were addressed. The concentration of the quality control samples calculated against the calibration curves were within the ±20% acceptance range. It was also demonstrated that heavy labeled peptides exhibit a similar extraction recovery and matrix effect to light ones. Consequently, the approach presented here may be a viable and cost-effective alternative of stable isotope labeling strategies for the quantification of cysteine-containing proteins. LA - English DB - MTMT ER - TY - JOUR AU - el Battioui, Kamal AU - Chakraborty, Sohini AU - Wacha, András AU - Molnár, Dániel AU - Quemé-Peña, Mayra AU - Szigyártó, Imola Cs. AU - Szabó, Csenge Lilla AU - Bodor, Andrea AU - Horváti, Kata AU - Gyulai, Gergő AU - Bősze, Szilvia AU - Mihály, Judith AU - Jezsó, Bálint AU - Románszki, Loránd AU - Tóth, Judit AU - Varga, Zoltán AU - Mándity, István AU - Juhász, Tünde AU - Beke-Somfai, Tamás TI - In situ captured antibacterial action of membrane-incising peptide lamellae JF - NATURE COMMUNICATIONS J2 - NAT COMMUN VL - 15 PY - 2024 IS - 1 PG - 14 SN - 2041-1723 DO - 10.1038/s41467-024-47708-4 UR - https://m2.mtmt.hu/api/publication/34819821 ID - 34819821 AB - Developing unique mechanisms of action are essential to combat the growing issue of antimicrobial resistance. Supramolecular assemblies combining the improved biostability of non-natural compounds with the complex membrane-attacking mechanisms of natural peptides are promising alternatives to conventional antibiotics. However, for such compounds the direct visual insight on antibacterial action is still lacking. Here we employ a design strategy focusing on an inducible assembly mechanism and utilized electron microscopy (EM) to follow the formation of supramolecular structures of lysine-rich heterochiral β 3 -peptides, termed lamellin-2K and lamellin-3K, triggered by bacterial cell surface lipopolysaccharides. Combined molecular dynamics simulations, EM and bacterial assays confirmed that the phosphate-induced conformational change on these lamellins led to the formation of striped lamellae capable of incising the cell envelope of Gram-negative bacteria thereby exerting antibacterial activity. Our findings also provide a mechanistic link for membrane-targeting agents depicting the antibiotic mechanism derived from the in-situ formation of active supramolecules. LA - English DB - MTMT ER - TY - JOUR AU - Szederkényi, G. AU - Kocsis, Dorottya AU - Vághy, M.A. AU - Czárán, Domonkos Tamás AU - Sasvári, Péter AU - Lengyel, Miléna AU - Naszlady, M.B. AU - Kreis, F. AU - Antal, István AU - Csépányi-Kömi, Roland AU - Erdő, F. TI - Mathematical modeling of transdermal delivery of topical drug formulations in a dynamic microfluidic diffusion chamber in health and disease JF - PLOS ONE J2 - PLOS ONE VL - 19 PY - 2024 IS - 4 PG - 17 SN - 1932-6203 DO - 10.1371/journal.pone.0299501 UR - https://m2.mtmt.hu/api/publication/34813473 ID - 34813473 AB - Mathematical models of epidermal and dermal transport are essential for optimization and development of products for percutaneous delivery both for local and systemic indication and for evaluation of dermal exposure to chemicals for assessing their toxicity. These models often help directly by providing information on the rate of drug penetration through the skin and thus on the dermal or systemic concentration of drugs which is the base of their pharmacological effect. The simulations are also helpful in analyzing experimental data, reducing the number of experiments and translating the in vitro investigations to an in-vivo setting. In this study skin penetration of topically administered caffeine cream was investigated in a skin-on-a-chip microfluidic diffusion chamber at room temperature and at 32̊C. Also the transdermal penetration of caffeine in healthy and diseased conditions was compared in mouse skins from intact, psoriatic and allergic animals. In the last experimental setup dexamethasone, indomethacin, piroxicam and diclofenac were examined as a cream formulation for absorption across the dermal barrier. All the measured data were used for making mathematical simulation in a three-compartmental model. The calculated and measured results showed a good match, which findings indicate that our mathematical model might be applied for prediction of drug delivery through the skin under different circumstances and for various drugs in the novel, miniaturized diffusion chamber. © 2024 Szederkényi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, LA - English DB - MTMT ER - TY - JOUR AU - Kalydi, Eszter AU - Malanga, Milo AU - Nielsen, Thorbjørn Terndrup AU - Wimmer, Reinhard AU - Béni, Szabolcs TI - Solving the puzzle of 2-hydroxypropyl β-cyclodextrin: Detailed assignment of the substituent distribution by NMR spectroscopy JF - CARBOHYDRATE POLYMERS J2 - CARBOHYD POLYM PY - 2024 SN - 0144-8617 DO - 10.1016/j.carbpol.2024.122167 UR - https://m2.mtmt.hu/api/publication/34804378 ID - 34804378 LA - English DB - MTMT ER - TY - JOUR AU - Kirschfink, M. AU - Frazer-Abel, A. AU - Bertalanné Balogh, Emese AU - Goseberg, S. AU - Weiss, N. AU - Prohászka, Zoltán TI - External quality assurance program for diagnostic complement laboratories: evaluation of the results of the past seven years JF - FRONTIERS IN IMMUNOLOGY J2 - FRONT IMMUNOL VL - 15 PY - 2024 PG - 18 SN - 1664-3224 DO - 10.3389/fimmu.2024.1368399 UR - https://m2.mtmt.hu/api/publication/34796435 ID - 34796435 LA - English DB - MTMT ER - TY - JOUR AU - Eszlári, Nóra AU - Hullám, Gábor István AU - Gál, Zsófia AU - Török, Dóra AU - Nagy, Tamás AU - Millinghoffer, András Dániel AU - Baksa, Dániel AU - Gonda, Xénia AU - Antal, Péter AU - Bagdy, György AU - Juhász, Gabriella TI - Olfactory genes affect major depression in highly educated, emotionally stable, lean women: a bridge between animal models and precision medicine JF - TRANSLATIONAL PSYCHIATRY J2 - TRANSL PSYCHIAT VL - 14 PY - 2024 IS - 1 PG - 10 SN - 2158-3188 DO - 10.1038/s41398-024-02867-2 UR - https://m2.mtmt.hu/api/publication/34779723 ID - 34779723 N1 - Funding Agency and Grant Number: Hungarian National Research, Development, and Innovation Office [K 139330, K 143391, PD 146014, 2019-2.1.7-ERA-NET-2020-00005, ERAPERMED2019-108]; Hungarian Brain Research Program [2017-1.2.1-NKP-2017-00002]; Hungarian Brain Research Program 3.0 [NAP2022-I-4/2022, TKP2021-EGA-25]; Ministry of Innovation and Technology of Hungary National Research, Development and Innovation Fund [TKP2021-EGA-25]; National Research, Development, and Innovation Fund of Hungary [TKP2021-EGA-02]; European Union [RRF-2.3.1-21-2022-00004]; New National Excellence Program of the Ministry for Culture and Innovation National Research, Development and Innovation Fund [UNKP-23-4-II-SE-2]; Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; Semmelweis University; [UNKP-22-4-II-SE-1] Funding text: This study was supported by the Hungarian National Research, Development, and Innovation Office, with grants K 139330, K 143391, and PD 146014, as well as 2019-2.1.7-ERA-NET-2020-00005 under the frame of ERA PerMed (ERAPERMED2019-108); by the Hungarian Brain Research Program (grant: 2017-1.2.1-NKP-2017-00002) and the Hungarian Brain Research Program 3.0 (NAP2022-I-4/2022); and by TKP2021-EGA-25, implemented with the support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund, financed under the TKP2021-EGA funding scheme. It was also supported by the National Research, Development, and Innovation Fund of Hungary under Grant TKP2021-EGA-02 and the European Union project RRF-2.3.1-21-2022-00004 within the framework of the Artificial Intelligence National Laboratory. NE was supported by the UNKP-22-4-II-SE-1, and DB by the UNKP-23-4-II-SE-2 New National Excellence Program of the Ministry for Culture and Innovation from the source of the National Research, Development and Innovation Fund. NE is supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences. This work uses data provided by patients and collected by the NHS as part of their care and support. Copyright (c) (2019), NHS England. Re-used with the permission of the UK Biobank (Application Number 1602). All rights reserved.Open access funding provided by Semmelweis University. AB - Most current approaches to establish subgroups of depressed patients for precision medicine aim to rely on biomarkers that require highly specialized assessment. Our present aim was to stratify participants of the UK Biobank cohort based on three readily measurable common independent risk factors, and to investigate depression genomics in each group to discover common and separate biological etiology. Two-step cluster analysis was run separately in males ( n = 149,879) and females ( n = 174,572), with neuroticism (a tendency to experience negative emotions), body fat percentage, and years spent in education as input variables. Genome-wide association analyses were implemented within each of the resulting clusters, for the lifetime occurrence of either a depressive episode or recurrent depressive disorder as the outcome. Variant-based, gene-based, gene set-based, and tissue-specific gene expression test were applied. Phenotypically distinct clusters with high genetic intercorrelations in depression genomics were found. A two-cluster solution was the best model in each sex with some differences including the less important role of neuroticism in males. In females, in case of a protective pattern of low neuroticism, low body fat percentage, and high level of education, depression was associated with pathways related to olfactory function. While also in females but in a risk pattern of high neuroticism, high body fat percentage, and less years spent in education, depression showed association with complement system genes. Our results, on one hand, indicate that alteration of olfactory pathways, that can be paralleled to the well-known rodent depression models of olfactory bulbectomy, might be a novel target towards precision psychiatry in females with less other risk factors for depression. On the other hand, our results in multi-risk females may provide a special case of immunometabolic depression. LA - English DB - MTMT ER - TY - JOUR AU - Gáborová, Mária AU - Vágvölgyi, Máté AU - Tayeb, Bizhar Ahmed AU - Minorics, Renáta AU - Zupkó, István AU - Jurček, Ondřej AU - Béni, Szabolcs AU - Kubínová, Renata AU - Balogh, György Tibor AU - Hunyadi, Attila TI - Diterpenes Isolated from Three Different Plectranthus Sensu Lato Species and Their Antiproliferative Activities against Gynecological and Glioblastoma Cancer Cells JF - ACS OMEGA J2 - ACS OMEGA VL - 9 PY - 2024 IS - 16 SP - 18495 EP - 18504 PG - 10 SN - 2470-1343 DO - 10.1021/acsomega.4c00800 UR - https://m2.mtmt.hu/api/publication/34779108 ID - 34779108 LA - English DB - MTMT ER - TY - JOUR AU - Gulyás, Eszter AU - Horváth, István László AU - Engh, Marie Anne AU - Bunduc, Stefania AU - Dembrovszky, Fanni AU - Fehérvári, Péter AU - Bánvölgyi, András AU - Csupor, Dezső AU - Hegyi, Péter AU - Karvaly, Gellért Balázs TI - Assessment of the practical impact of adjusting beta-lactam dosages based on therapeutic drug monitoring in critically ill adult patients: a systematic review and meta-analysis of randomized clinical trials and observational studies JF - SCIENTIFIC REPORTS J2 - SCI REP VL - 14 PY - 2024 IS - 1 PG - 13 SN - 2045-2322 DO - 10.1038/s41598-024-58200-w UR - https://m2.mtmt.hu/api/publication/34776969 ID - 34776969 N1 - Centre for Translational Medicine, Semmelweis University, Budapest, Hungary University Pharmacy Department of Pharmacy Administration, Semmelweis University, Budapest, Hungary Department of Laboratory Medicine, Semmelweis University, 4 Nagyvarad ter, Budapest, 1089, Hungary Institute for Translational Medicine, Medical School, University of Pécs, Pécs, Hungary Carol Davila University of Medicine and Pharmacy, Bucharest, Romania Fundeni Clinical Institute, Bucharest, Romania First Department of Medicine, University of Pécs, Pécs, Hungary János Szentágothai Research Center, University of Pécs, Pécs, Hungary Department of Biostatistics, University of Veterinary Medicine, Budapest, Hungary Department of Dermatology, Venereology and Dermatooncology, Semmelweis University, Budapest, Hungary Department of Clinical Pharmacy, University of Szeged, Szeged, Hungary Division of Pancreatic Diseases, Heart and Vascular Center, Semmelweis University, Budapest, Hungary Export Date: 12 April 2024 Correspondence Address: Karvaly, G.B.; Centre for Translational Medicine, Hungary; email: karvaly.gellert.balazs@semmelweis.hu LA - English DB - MTMT ER -