@article{MTMT:34821651,
	title = {A Mass Spectrometry Strategy for Protein Quantification Based on the Differential Alkylation of Cysteines Using Iodoacetamide and Acrylamide},
	url = {https://m2.mtmt.hu/api/publication/34821651},
	author = {Virág, Dávid and Schlosser, Gitta and Borbély, Adina and Gellén, Gabriella and Papp, Dávid and Kaleta, Zoltán and Dalmadiné Kiss, Borbála and Antal, István and Ludányi, Krisztina},
	doi = {10.3390/ijms25094656},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34821651},
	issn = {1661-6596},
	abstract = {Mass spectrometry has become the most prominent yet evolving technology in quantitative proteomics. Today, a number of label-free and label-based approaches are available for the relative and absolute quantification of proteins and peptides. However, the label-based methods rely solely on the employment of stable isotopes, which are expensive and often limited in availability. Here we propose a label-based quantification strategy, where the mass difference is identified by the differential alkylation of cysteines using iodoacetamide and acrylamide. The alkylation reactions were performed under identical experimental conditions; therefore, the method can be easily integrated into standard proteomic workflows. Using high-resolution mass spectrometry, the feasibility of this approach was assessed with a set of tryptic peptides of human serum albumin. Several critical questions, such as the efficiency of labeling and the effect of the differential alkylation on the peptide retention and fragmentation, were addressed. The concentration of the quality control samples calculated against the calibration curves were within the ±20% acceptance range. It was also demonstrated that heavy labeled peptides exhibit a similar extraction recovery and matrix effect to light ones. Consequently, the approach presented here may be a viable and cost-effective alternative of stable isotope labeling strategies for the quantification of cysteine-containing proteins.},
	year = {2024},
	eissn = {1422-0067},
	pages = {4656},
	orcid-numbers = {Virág, Dávid/0000-0001-8411-0773; Schlosser, Gitta/0000-0002-7637-7133; Gellén, Gabriella/0000-0002-9048-2722; Papp, Dávid/0000-0002-2006-7777; Kaleta, Zoltán/0000-0003-2350-5100; Dalmadiné Kiss, Borbála/0000-0002-5774-7880; Antal, István/0000-0002-5434-201X; Ludányi, Krisztina/0000-0002-2380-9529}
}

@article{MTMT:34813473,
	title = {Mathematical modeling of transdermal delivery of topical drug formulations in a dynamic microfluidic diffusion chamber in health and disease},
	url = {https://m2.mtmt.hu/api/publication/34813473},
	author = {Szederkényi, G. and Kocsis, D. and Vághy, M.A. and Czárán, Domonkos Tamás and Sasvári, Péter and Lengyel, Miléna and Naszlady, M.B. and Kreis, F. and Antal, István and Csépányi-Kömi, Roland and Erdő, F.},
	doi = {10.1371/journal.pone.0299501},
	journal-iso = {PLOS ONE},
	journal = {PLOS ONE},
	volume = {19},
	unique-id = {34813473},
	issn = {1932-6203},
	abstract = {Mathematical models of epidermal and dermal transport are essential for optimization and development of products for percutaneous delivery both for local and systemic indication and for evaluation of dermal exposure to chemicals for assessing their toxicity. These models often help directly by providing information on the rate of drug penetration through the skin and thus on the dermal or systemic concentration of drugs which is the base of their pharmacological effect. The simulations are also helpful in analyzing experimental data, reducing the number of experiments and translating the in vitro investigations to an in-vivo setting. In this study skin penetration of topically administered caffeine cream was investigated in a skin-on-a-chip microfluidic diffusion chamber at room temperature and at 32̊C. Also the transdermal penetration of caffeine in healthy and diseased conditions was compared in mouse skins from intact, psoriatic and allergic animals. In the last experimental setup dexamethasone, indomethacin, piroxicam and diclofenac were examined as a cream formulation for absorption across the dermal barrier. All the measured data were used for making mathematical simulation in a three-compartmental model. The calculated and measured results showed a good match, which findings indicate that our mathematical model might be applied for prediction of drug delivery through the skin under different circumstances and for various drugs in the novel, miniaturized diffusion chamber. © 2024 Szederkényi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License,},
	keywords = {ABSORPTION; DEXAMETHASONE; ARTICLE; MOUSE; PREDICTION; nonhuman; in vitro study; simulation; drug formulation; drug delivery system; indometacin; room temperature; CAFFEINE; diclofenac; mathematical model; piroxicam; drug penetration; cream; Product development; diffusion chamber; psoriasis; Epidermis; compartment model; skin penetration; dermis; skin on a chip},
	year = {2024},
	eissn = {1932-6203},
	orcid-numbers = {Lengyel, Miléna/0000-0001-8865-054X; Antal, István/0000-0002-5434-201X; Csépányi-Kömi, Roland/0000-0001-6825-7142}
}

@article{MTMT:34796435,
	title = {External quality assurance program for diagnostic complement laboratories: evaluation of the results of the past seven years},
	url = {https://m2.mtmt.hu/api/publication/34796435},
	author = {Kirschfink, M. and Frazer-Abel, A. and Bertalanné Balogh, Emese and Goseberg, S. and Weiss, N. and Prohászka, Zoltán},
	doi = {10.3389/fimmu.2024.1368399},
	journal-iso = {FRONT IMMUNOL},
	journal = {FRONTIERS IN IMMUNOLOGY},
	volume = {15},
	unique-id = {34796435},
	issn = {1664-3224},
	year = {2024},
	eissn = {1664-3224},
	orcid-numbers = {Bertalanné Balogh, Emese/0000-0002-1127-3923; Prohászka, Zoltán/0000-0003-1761-7982}
}

@article{MTMT:34779723,
	title = {Olfactory genes affect major depression in highly educated, emotionally stable, lean women: a bridge between animal models and precision medicine},
	url = {https://m2.mtmt.hu/api/publication/34779723},
	author = {Eszlári, Nóra and Hullám, Gábor István and Gál, Zsófia and Török, Dóra and Nagy, Tamás and Millinghoffer, András Dániel and Baksa, Dániel and Gonda, Xénia and Antal, Péter and Bagdy, György and Juhász, Gabriella},
	doi = {10.1038/s41398-024-02867-2},
	journal-iso = {TRANSL PSYCHIAT},
	journal = {TRANSLATIONAL PSYCHIATRY},
	volume = {14},
	unique-id = {34779723},
	issn = {2158-3188},
	abstract = {Most current approaches to establish subgroups of depressed patients for precision medicine aim to rely on biomarkers that require highly specialized assessment. Our present aim was to stratify participants of the UK Biobank cohort based on three readily measurable common independent risk factors, and to investigate depression genomics in each group to discover common and separate biological etiology. Two-step cluster analysis was run separately in males ( n  = 149,879) and females ( n  = 174,572), with neuroticism (a tendency to experience negative emotions), body fat percentage, and years spent in education as input variables. Genome-wide association analyses were implemented within each of the resulting clusters, for the lifetime occurrence of either a depressive episode or recurrent depressive disorder as the outcome. Variant-based, gene-based, gene set-based, and tissue-specific gene expression test were applied. Phenotypically distinct clusters with high genetic intercorrelations in depression genomics were found. A two-cluster solution was the best model in each sex with some differences including the less important role of neuroticism in males. In females, in case of a protective pattern of low neuroticism, low body fat percentage, and high level of education, depression was associated with pathways related to olfactory function. While also in females but in a risk pattern of high neuroticism, high body fat percentage, and less years spent in education, depression showed association with complement system genes. Our results, on one hand, indicate that alteration of olfactory pathways, that can be paralleled to the well-known rodent depression models of olfactory bulbectomy, might be a novel target towards precision psychiatry in females with less other risk factors for depression. On the other hand, our results in multi-risk females may provide a special case of immunometabolic depression.},
	year = {2024},
	eissn = {2158-3188},
	orcid-numbers = {Eszlári, Nóra/0000-0003-4913-028X; Hullám, Gábor István/0000-0002-4765-2351; Gál, Zsófia/0000-0002-9441-1497; Török, Dóra/0000-0001-9213-4345; Nagy, Tamás/0000-0002-0137-4341; Baksa, Dániel/0000-0002-7826-9179; Gonda, Xénia/0000-0001-9015-4203; Bagdy, György/0000-0001-8141-3410; Juhász, Gabriella/0000-0002-5975-4267}
}

@article{MTMT:34779108,
	title = {Diterpenes Isolated from Three Different Plectranthus Sensu Lato Species and Their Antiproliferative Activities against Gynecological and Glioblastoma Cancer Cells},
	url = {https://m2.mtmt.hu/api/publication/34779108},
	author = {Gáborová, Mária and Vágvölgyi, Máté and Tayeb, Bizhar Ahmed and Minorics, Renáta and Zupkó, István and Jurček, Ondřej and Béni, Szabolcs and Kubínová, Renata and Balogh, György Tibor and Hunyadi, Attila},
	doi = {10.1021/acsomega.4c00800},
	journal-iso = {ACS OMEGA},
	journal = {ACS OMEGA},
	unique-id = {34779108},
	issn = {2470-1343},
	year = {2024},
	eissn = {2470-1343},
	orcid-numbers = {Vágvölgyi, Máté/0000-0002-2233-9422; Tayeb, Bizhar Ahmed/0000-0001-5197-564X; Minorics, Renáta/0000-0001-9685-813X; Zupkó, István/0000-0003-3243-5300; Béni, Szabolcs/0000-0001-7056-6825; Balogh, György Tibor/0000-0003-3347-1880; Hunyadi, Attila/0000-0003-0074-3472}
}

@article{MTMT:34776969,
	title = {Assessment of the practical impact of adjusting beta-lactam dosages based on therapeutic drug monitoring in critically ill adult patients: a systematic review and meta-analysis of randomized clinical trials and observational studies},
	url = {https://m2.mtmt.hu/api/publication/34776969},
	author = {Gulyás, Eszter and Horváth, István László and Engh, Marie Anne and Bunduc, Stefania and Dembrovszky, Fanni and Fehérvári, Péter and Bánvölgyi, András and Csupor, Dezső and Hegyi, Péter and Karvaly, Gellért Balázs},
	doi = {10.1038/s41598-024-58200-w},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {14},
	unique-id = {34776969},
	issn = {2045-2322},
	year = {2024},
	eissn = {2045-2322},
	orcid-numbers = {Engh, Marie Anne/0000-0003-4269-5130; Dembrovszky, Fanni/0000-0001-6953-3591; Bánvölgyi, András/0000-0002-7071-1364; Csupor, Dezső/0000-0002-4088-3333; Hegyi, Péter/0000-0003-0399-7259; Karvaly, Gellért Balázs/0000-0003-2468-5633}
}

@article{MTMT:34768534,
	title = {A Clinical, Pharmacological, and Formulation Evaluation of Melatonin in the Treatment of Ocular Disorders—A Systematic Review},
	url = {https://m2.mtmt.hu/api/publication/34768534},
	author = {Romeo, Alessia and Nochta-Kazsoki, Adrienn Katalin and Musumeci, Teresa and Zelkó, Romána},
	doi = {10.3390/ijms25073999},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34768534},
	issn = {1661-6596},
	abstract = {Melatonin’s cytoprotective properties may have therapeutic implications in treating ocular diseases like glaucoma and age-related macular degeneration. Literature data suggest that melatonin could potentially protect ocular tissues by decreasing the production of free radicals and pro-inflammatory mediators. This study aims to summarize the screened articles on melatonin’s clinical, pharmacological, and formulation evaluation in treating ocular disorders. The identification of relevant studies on the topic in focus was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA 2020) guidelines. The studies were searched in the following databases and web search engines: Pubmed, Scopus, Science Direct, Web of Science, Reaxys, Google Scholar, Google Patents, Espacenet, and Patentscope. The search time interval was 2013–2023, with the following keywords: melatonin AND ocular OR ophthalmic AND formulation OR insert AND disease. Our key conclusion was that using melatonin-loaded nano-delivery systems enabled the improved permeation of the molecule into intraocular tissues and assured controlled release profiles. Although preclinical studies have demonstrated the efficacy of developed formulations, a considerable gap has been observed in the clinical translation of the results. To overcome this failure, revising the preclinical experimental phase might be useful by selecting endpoints close to clinical ones.},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Nochta-Kazsoki, Adrienn Katalin/0000-0002-0611-3124; Musumeci, Teresa/0000-0003-2299-7780; Zelkó, Romána/0000-0002-5419-9137}
}

@article{MTMT:34757551,
	title = {Optical Investigation of 2-amino-7-isocyanofluorene, a Novel Blue-Emitting Solvatochromic Dye},
	url = {https://m2.mtmt.hu/api/publication/34757551},
	author = {Kontra, Bence and Mucsi, Zoltán and Vanyorek, László and Nagy, Miklós},
	doi = {10.3390/colorants3020006},
	journal-iso = {Colorants},
	journal = {COLORANTS},
	volume = {3},
	unique-id = {34757551},
	abstract = {Smart solvatochromic isocyano-aminoarenes (ICAArs) have been gaining attention owing to their unique photophysical, antifungal and anticancer properties. Using a simple dehydration reaction with in situ-generated dichlorocarbene, we prepared 2-amino-7-isocyanofluorene (2,7-ICAF). We studied the effect of the longer polarization axis provided by the fluorene core on the spectral properties and we also compared it to those of the starting diamine. 2,7-ICAF shows a clear solvatochromic behavior close to the blue part (370–420 nm) of the visible spectrum. Quantum chemical calculations show internal charge transfer (ICT) between the donor amino and the electron-withdrawing isocyano groups. 2,7-ICAF has high molar absorptivity (ε = 15–18·103 M−1cm−1) and excellent quantum yield (Φf = 70–95%) in most solvents; however, its fluorescence is completely quenched in water. The high brightness (ε·Φf) and close to zero quantum yield in water may be favorable in biolabeling applications, where background fluorescence should be kept minimal. Overall, 2,7-ICAF shows enhanced photophysical properties compared to its previously investigated relative 4-amino-4′-isocyano-1,1′-biphenyl (4,4′-ICAB).},
	year = {2024},
	eissn = {2079-6447},
	pages = {86-98},
	orcid-numbers = {Kontra, Bence/0000-0001-8293-3637; Mucsi, Zoltán/0000-0003-3224-8847; Nagy, Miklós/0000-0002-3484-2244}
}

@article{MTMT:34753258,
	title = {Gram-negative rough mutants used as test bacteria can increase sensitivity of direct bioautography},
	url = {https://m2.mtmt.hu/api/publication/34753258},
	author = {Balázs, Viktória Lilla and Böszörményi, Andrea and Kocsis, Béla and Horváth, Györgyi},
	doi = {10.1007/s00764-024-00293-0},
	journal-iso = {J PLANAR CHROMATOGR MOD TLC},
	journal = {JPC - JOURNAL OF PLANAR CHROMATOGRAPHY - MODERN TLC},
	unique-id = {34753258},
	issn = {0933-4173},
	abstract = {Currently, the antimicrobial activity of essential oils (EOs) is an outstanding research field due to antibiotic resistance of microorganisms. Thin-layer chromatography‒direct bioautography (TLC‒DB) is an effective, fast method to find components with antimicrobial activity in a mixture of plant compounds, e.g., in EOs. The volatility and hydrophobic characters of EOs require special experimental conditions, and disc diffusion assay is not appropriate to explore the antimicrobial activity of them. The aim of this study was to use “R” mutants, which are more sensitive to synthetic antimicrobial drugs, in DB to increase the sensitivity of this method. Our hypothesis was that these mutants show sensitivity to some EOs (thyme, clove, and peppermint) as well. The chemical composition of our tested EOs was measured with gas chromatography‒mass spectrometry (GC‒MS). The main compounds (39.8% thymol, 78.8% eugenol, and 50.4% menthol) of EOs showed notable antibacterial activity in TLC‒DB. Based on our results, we suggest to use Salmonella minnesota Re595 rough strain as test bacterium in bioautography, because it showed the highest sensitivity to the tested antibiotics (gentamicin and cephalexin) and EOs. Furthermore, this rough mutant could make TLC‒DB more faster, because only 4 h incubation time was enough to detect the inhibition zones of the active compounds used in this study.},
	year = {2024},
	eissn = {1789-0993},
	orcid-numbers = {Böszörményi, Andrea/0000-0003-3982-7059; Horváth, Györgyi/0000-0001-5344-0294}
}

@article{MTMT:34752694,
	title = {The relationship between alexithymia, rumination and binge drinking among university students},
	url = {https://m2.mtmt.hu/api/publication/34752694},
	author = {Alpay, Pelin and Kocsel, Natália and Galambos, Attila and Kökönyei, Gyöngyi},
	doi = {10.1016/j.paid.2024.112621},
	journal-iso = {PERS INDIV DIFFER},
	journal = {PERSONALITY AND INDIVIDUAL DIFFERENCES},
	volume = {223},
	unique-id = {34752694},
	issn = {0191-8869},
	year = {2024},
	eissn = {1873-3549},
	orcid-numbers = {Kökönyei, Gyöngyi/0000-0001-6750-2644}
}