@article{MTMT:34796888, title = {Decidual γδT cells of early human pregnancy produce angiogenic and immunomodulatory proteins while also possessing cytotoxic potential}, url = {https://m2.mtmt.hu/api/publication/34796888}, author = {Nörenberg, Jasper Maximilian and Vida, P. and Bösmeier, I. and Forró, B. and Nörenberg, A. and Buda, Á. and Simon, Diána and Erdő-Bonyár, Szabina and Jáksó, Pál and Kovács, Kálmán András and Mikó, Éva and Berki, Tímea and Mezősi, Emese and Barakonyi, Alíz}, doi = {10.3389/fimmu.2024.1382424}, journal-iso = {FRONT IMMUNOL}, journal = {FRONTIERS IN IMMUNOLOGY}, volume = {15}, unique-id = {34796888}, issn = {1664-3224}, year = {2024}, eissn = {1664-3224}, orcid-numbers = {Berki, Tímea/0000-0002-0134-8127; Mezősi, Emese/0000-0001-9367-3877} } @article{MTMT:34757150, title = {Co-infecting viruses of species Bovine rhinitis B virus (Picornaviridae) and Bovine nidovirus 1 (Tobaniviridae) identified for the first time from a post-mortem respiratory sample of a sheep (Ovis aries) in Hungary}, url = {https://m2.mtmt.hu/api/publication/34757150}, author = {Tóth, Fruzsina and Gáspár, Gábor and Pankovics, Péter and Urbán, Péter and Herczeg, Róbert and Albert, Mihály and Reuter, Gábor and Boros, Ákos}, doi = {10.1016/j.meegid.2024.105585}, journal-iso = {INFECT GENET EVOL}, journal = {INFECTION GENETICS AND EVOLUTION}, volume = {120}, unique-id = {34757150}, issn = {1567-1348}, abstract = {In this study, a picornavirus and a nidovirus were identified from a single available nasopharyngeal swab (NPS) sample of a freshly deceased sheep, as the only vertebrate viruses found with viral metagenomics and next-generation sequencing methods. The sample was originated from a mixed feedlot farm in Hungary where sheep and cattle were held together but in separate stalls. Most of the sheep had respiratory signs (coughing and increased respiratory effort) at the time of sampling. Other NPS were not, but additional enteric samples were collected from sheep (n = 27) and cattle (n = 11) of the same farm at that time. The complete/nearly complete genomes of the identified viruses were determined using RT-PCR and Nanopore (MinION-Flonge) / Dye-terminator sequencing techniques. The results of detailed genomic and phylogenetic analyses indicate that the identified picornavirus most likely belongs to a type 4 genotype of species Bovine rhinitis B virus (BRBV-4, OR885914) of genus Aphthovirus, family Picornaviridae while the ovine nidovirus (OvNV, OR885915) - as a novel variant - could belong to the recently created Bovine nidovirus 1 (BoNV) species of genus Bostovirus, family Tobaniviridae. None of the identified viruses were detectable in the enteric samples using RT-PCR and generic screening primer pairs. Both viruses are well-known respiratory pathogens of cattle, but their presence was not demonstrated before in other animals, like sheep. Furthermore, neither BRBV-4 nor BoNVs were investigated in European cattle and/or sheep flocks, therefore it cannot be determined whether the presence of these viruses in sheep was a result of a single host species switch/spillover event or these viruses are circulating in not just cattle but sheep populations as well. Further studies required to investigate the spread of these viruses in Hungarian and European sheep and cattle populations and to identify their pathogenic potential in sheep.}, keywords = {SHEEP; Respiratory disease; Picornavirus; Co-infection; Spillover; nidovirus; Bovine rhinitis B virus}, year = {2024}, eissn = {1567-7257}, orcid-numbers = {Herczeg, Róbert/0000-0002-5903-0082; Reuter, Gábor/0000-0002-5857-4934; Boros, Ákos/0000-0001-5843-4360} } @article{MTMT:34753258, title = {Gram-negative rough mutants used as test bacteria can increase sensitivity of direct bioautography}, url = {https://m2.mtmt.hu/api/publication/34753258}, author = {Balázs, Viktória Lilla and Böszörményi, Andrea and Kocsis, Béla and Horváth, Györgyi}, doi = {10.1007/s00764-024-00293-0}, journal-iso = {J PLANAR CHROMATOGR MOD TLC}, journal = {JPC - JOURNAL OF PLANAR CHROMATOGRAPHY - MODERN TLC}, unique-id = {34753258}, issn = {0933-4173}, abstract = {Currently, the antimicrobial activity of essential oils (EOs) is an outstanding research field due to antibiotic resistance of microorganisms. Thin-layer chromatography‒direct bioautography (TLC‒DB) is an effective, fast method to find components with antimicrobial activity in a mixture of plant compounds, e.g., in EOs. The volatility and hydrophobic characters of EOs require special experimental conditions, and disc diffusion assay is not appropriate to explore the antimicrobial activity of them. The aim of this study was to use “R” mutants, which are more sensitive to synthetic antimicrobial drugs, in DB to increase the sensitivity of this method. Our hypothesis was that these mutants show sensitivity to some EOs (thyme, clove, and peppermint) as well. The chemical composition of our tested EOs was measured with gas chromatography‒mass spectrometry (GC‒MS). The main compounds (39.8% thymol, 78.8% eugenol, and 50.4% menthol) of EOs showed notable antibacterial activity in TLC‒DB. Based on our results, we suggest to use Salmonella minnesota Re595 rough strain as test bacterium in bioautography, because it showed the highest sensitivity to the tested antibiotics (gentamicin and cephalexin) and EOs. Furthermore, this rough mutant could make TLC‒DB more faster, because only 4 h incubation time was enough to detect the inhibition zones of the active compounds used in this study.}, year = {2024}, eissn = {1789-0993}, orcid-numbers = {Böszörményi, Andrea/0000-0003-3982-7059; Horváth, Györgyi/0000-0001-5344-0294} } @article{MTMT:34745300, title = {Antibiotics and probiotics-induced effects on the total fatty acid composition of feces in a rat model}, url = {https://m2.mtmt.hu/api/publication/34745300}, author = {Marosvölgyi, Tamás and Mintál, Kitti and Borbásné Farkas, Kornélia and Sipos, Zoltán and Makszin, Lilla and Szabó, Éva and Tóth, Attila and Kocsis, Béla and Kovács, Krisztina and Hormay, Edina and Lénárd, László and Karádi, Zoltán György and Bufa, Anita}, doi = {10.1038/s41598-024-57046-6}, journal-iso = {SCI REP}, journal = {SCIENTIFIC REPORTS}, volume = {14}, unique-id = {34745300}, issn = {2045-2322}, abstract = {Fatty acids (FAs) play important roles as membrane components and signal transduction molecules. Changes in short chain FA (SCFA) composition are associated with gut microbiota modifications. However, the effect of bacteria-driven changes on the detailed FA spectrum has not been explored yet. We investigated the effect of antibiotics (ABx) and/or probiotics, in four treatment groups on rat stool FA composition. Principal component analysis indicated that the chromatogram profiles of the treatment groups differ, which was also observed at different time points. Linear mixed effects models showed that in the parameters compared (sampling times, treatments. and their interactions), both the weight percentage and the concentration of FAs were affected by ABx and probiotic administration. This study found that the gut microbiome defines trans and branched saturated FAs, most saturated FAs, and unsaturated FAs with less carbon atoms. These results are among the first ones to demonstrate the restoring effects of a probiotic mixture on a substantial part of the altered total FA spectrum, and also revealed a previously unknown relationship between gut bacteria and a larger group of FAs. These findings suggest that intestinal bacteria produce not only SCFAs but also other FAs that may affect the host’s physiological processes.}, year = {2024}, eissn = {2045-2322}, orcid-numbers = {Marosvölgyi, Tamás/0000-0002-4244-5513; Mintál, Kitti/0009-0003-4739-2902; Borbásné Farkas, Kornélia/0000-0002-5349-6527; Sipos, Zoltán/0000-0001-7845-8116; Makszin, Lilla/0000-0002-9764-4763; Hormay, Edina/0009-0004-0598-5061} } @article{MTMT:34738553, title = {Chestnut Honey Is Effective against Mixed Biofilms at Different Stages of Maturity}, url = {https://m2.mtmt.hu/api/publication/34738553}, author = {Koloh, Regina and Balázs, Viktória Lilla and Radványi, Lilla and Kocsis, Béla and Kerekes, Erika Beáta and Kocsis, Marianna and Farkas, Ágnes}, doi = {10.3390/antibiotics13030255}, journal-iso = {ANTIBIOTICS-BASEL}, journal = {ANTIBIOTICS}, volume = {13}, unique-id = {34738553}, abstract = {The irresponsible overuse of antibiotics has increased the occurrence of resistant bacterial strains, which represents one of the biggest patient safety risks today. Due to antibiotic resistance and biofilm formation in bacteria, it is becoming increasingly difficult to suppress the bacterial strains responsible for various chronic infections. Honey was proven to inhibit bacterial growth and biofilm development, offering an alternative solution in the treatment of resistant infections and chronic wounds. Our studies included chestnut honey, valued for its high antibacterial activity, and the bacteria Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and S. epidermidis, known to form multi-species biofilm communities. Minimum inhibitory concentrations (MIC) of chestnut honey were determined for each bacterial strain. Afterwards, the mixed bacterial biofilms were treated with chestnut honey at different stages of maturity (incubation times: 2, 4, 6, 12, 24 h). The extent of biofilm inhibition was measured with a crystal violet assay and demonstrated by scanning electron microscopy (SEM). As the incubation time increased and the biofilm became more mature, inhibition rates decreased gradually. The most sensitive biofilm was the combination MRSA-S. epidermidis, with a 93.5% inhibition rate after 2 h of incubation. Our results revealed that chestnut honey is suitable for suppressing the initial and moderately mature stages of mixed biofilms.}, year = {2024}, eissn = {2079-6382}, orcid-numbers = {Balázs, Viktória Lilla/0000-0002-9526-0264; Kerekes, Erika Beáta/0000-0002-1245-6510; Kocsis, Marianna/0000-0002-1779-6370; Farkas, Ágnes/0000-0002-8590-3203} } @article{MTMT:34729562, title = {Incidental finding of a human-like tusavirus in a lamb with lip lesions and fatal pneumonia}, url = {https://m2.mtmt.hu/api/publication/34729562}, author = {Davies, Hannah and Dastjerdi, Akbar and Everest, David and Floyd, Tobias and Collins, Rachael and McFadzean, Harriet and Reuter, Gábor and Reichel, Rudolf}, doi = {10.1099/jgv.0.001968}, journal-iso = {J GEN VIROL}, journal = {JOURNAL OF GENERAL VIROLOGY}, volume = {105}, unique-id = {34729562}, issn = {0022-1317}, abstract = {Tusaviruses in the genus Protoparvovirus of family Parvoviridae were first identified in a diarrhoeic Tunisian child in 2014. Thereafter, high prevalence of a genetically similar virus was demonstrated in faeces from caprine and ovine species in Hungary. Here, we describe an investigation into the cause of scabby lip lesions in a 6 month-old lamb, submitted from a farm experiencing weight loss and scouring in lambs in England. Transmission electron microscopy visualised small circular particles of 18 and 22 nm in diameter in lip lesions identified as tusavirus and flumine parvovirus by Next Generation Sequencing. Liver, kidney, lung, small intestine content and faeces were also strongly positive for the tusavirus DNA as well as 10 % of faecal samples of the flock collected 2 months after the initial lip sampling. NS1 and VP1 amino acid sequences of this tusavirus displayed 99.5 and 92.89 % identity to those of a human tusavirus, respectively. These amino acid identities were at 95.5 and 89.68 % when compared to those of a goat tusavirus. Phylogenetic analysis of the NS1 and VP1 also grouped the virus in the genus Protoparvovirus and close to tusaviruses detected in human, ovine and caprine species. Wider surveillance of the virus indicated a broader geographical distribution for the virus in England. Histology of the lip tissue revealed localised areas of epidermal hyperplasia and hyperkeratosis affecting haired skin, with mild leucocyte infiltration of the subjacent dermis, but no changes to implicate virus involvement. Flumine parvovirus was concluded to be an environment contaminant. Broader studies in prevalence of these virus in UK sheep flocks and human population, animal models and experimental infections could provide insights into the pathogenesis of these novel viruses and their zoonotic potential.}, keywords = {pneumonia; protoparvovirus; lip lesions; ovine parvovirus; pustular dermatitis; tusavirus}, year = {2024}, eissn = {1465-2099}, orcid-numbers = {Reuter, Gábor/0000-0002-5857-4934} } @article{MTMT:34725854, title = {Human orf virus (family Poxviridae) infection following a lamb bite in Hungary}, url = {https://m2.mtmt.hu/api/publication/34725854}, author = {Németh, Csongor and Boros, Ákos and Mészáros, Endre and Gyömörei, Csaba and Albert, Ervin and Pankovics, Péter and Reuter, Gábor}, doi = {10.1007/s00705-024-06002-w}, journal-iso = {ARCH VIROL}, journal = {ARCHIVES OF VIROLOGY}, volume = {169}, unique-id = {34725854}, issn = {0304-8608}, abstract = {Human orf disease (called ecthyma contagiosum or contagious/infectious pustular dermatitis in animals) was confirmed on the fingers of both hands of a 24-year-old female, after feeding diseased lambs with a nursing bottle in April 2023. In addition to skin symptoms, she had low-grade fever (37.6°C) and swollen lymph nodes in both axilla. The presence of orf virus (genus Parapoxvirus, family Poxviridae) was confirmed, and this strain, Baja/2023/HUN (OR372161-OR372163), was found to have > 98% nucleotide sequence identity to sheep-origin orf viruses in four tested genome regions (ORF011/B2L, ORF019, ORF020/VIR, and ORF056). This is the first report of a human case of infection with the neglected zoonotic orf virus in Hungary.}, year = {2024}, eissn = {1432-8798}, orcid-numbers = {Boros, Ákos/0000-0001-5843-4360; Reuter, Gábor/0000-0002-5857-4934} } @article{MTMT:34721811, title = {Characterization of antibiotic and disinfectant susceptibility in biofilm-forming Acinetobacter baumannii: A focus on environmental isolates}, url = {https://m2.mtmt.hu/api/publication/34721811}, author = {Pallós, Péter and Gajdács, Márió and Zsoldiné Urbán, Edit and Szabados, Yvett and Szalai, Klaudia and Hevesi, Lívia and Horváth, Anna and Kuklis, Anna and Morjaria, Devina and Iffat, Wajiha and Hetta, Helal F. and Piredda, Nicola and Donadu, Matthew Gavino}, doi = {10.1556/1886.2024.00014}, journal-iso = {EUR J MICROBIOL IMMU}, journal = {EUROPEAN JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY}, unique-id = {34721811}, issn = {2062-509X}, abstract = {The clinical role of Acinetobacter baumannii has been highlighted in numerous infectious syndromes with a high mortality rate, due to the high prevalence of multidrug-resistant (MDR) isolates. The treatment and eradication of this pathogen is hindered by biofilm-formation, providing protection from noxious environmental factors and antimicrobials. The aim of this study was to assess the antibiotic susceptibility, antiseptic susceptibility and biofilm-forming capacity using phenotypic methods in environmental A. baumannii isolates. One hundred and fourteen ( n = 114) isolates were collected, originating from various environmental sources and geographical regions. Antimicrobial susceptibility testing was carried out using the disk diffusion method, while antiseptic susceptibility was performed using the agar dilution method. Determination of biofilm-forming capacity was carried out using a microtiter-plate based method. Resistance in environmental A. baumannii isolates were highest for ciprofloxacin (64.03%, n = 73), levofloxacin (62.18%, n = 71) and trimethoprim-sulfamethoxazole (61.40%, n = 70), while lowest for colistin (1.75%, n = 2). Efflux pump overexpression was seen in 48.25% of isolates ( n = 55), 49.12% ( n = 56) were classified as MDR. 6.14% ( n = 7), 9.65% ( n = 11), 24.65% ( n = 28) and 59.65% ( n = 68) of isolates were non-biofilm producers, weak, medium, and strong biofilm producers, respectively. No significant differences were observed between non-MDR vs. MDR isolates regarding their distribution of biofilm-producers ( P = 0.655). The MIC ranges for the tested antiseptics were as follows: benzalkonium chloride 16–128 μg mL −1 , chlorhexidine digluconate 4–128 μg mL −1 , formaldehyde 64–256 μg mL −1 and triclosan 2–16 μg mL −1 , respectively. The conscientious use of antiseptics, together with periodic surveillance, is essential to curb the spread of these bacteria, and to maintain current infection prevention capabilities.}, year = {2024}, eissn = {2062-8633}, orcid-numbers = {Gajdács, Márió/0000-0003-1270-0365; Zsoldiné Urbán, Edit/0000-0002-9602-7552} } @article{MTMT:34574199, title = {Radiation-Detoxified Form of Endotoxin Effectively Activates Th1 Responses and Attenuates Ragweed-Induced Th2-Type Airway Inflammation in Mice}, url = {https://m2.mtmt.hu/api/publication/34574199}, author = {Bácsi, Attila and Ágics, Beatrix and Pázmándi, Kitti Linda and Kocsis, Béla and Sándor, Viktor and Bertók, Lóránd and Bruckner, Geza and Sipka, Sándor}, doi = {10.3390/ijms25031581}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {25}, unique-id = {34574199}, issn = {1661-6596}, abstract = {Urbanization with reduced microbial exposure is associated with an increased burden of asthma and atopic symptoms. Conversely, environmental exposure to endotoxins in childhood can protect against the development of allergies. Our study aimed to investigate whether the renaturation of the indoor environment with aerosolized radiation-detoxified lipopolysaccharide (RD-LPS) has a preventative effect against the development of ragweed-induced Th2-type airway inflammation. To explore this, cages of six-week-old BALB/c mice were treated daily with aerosolized native LPS (N-LPS) or RD-LPS. After a 10-week treatment period, mice were sensitized and challenged with ragweed pollen extract, and inflammatory cell infiltration into the airways was observed. As dendritic cells (DCs) play a crucial role in the polarization of T-cell responses, in our in vitro experiments, the effects of N-LPS and RD-LPS were compared on human monocyte-derived DCs (moDCs). Mice in RD-LPS-rich milieu developed significantly less allergic airway inflammation than mice in N-LPS-rich or common environments. The results of our in vitro experiments demonstrate that RD-LPS-exposed moDCs have a higher Th1-polarizing capacity than moDCs exposed to N-LPS. Consequently, we suppose that the aerosolized, non-toxic RD-LPS applied in early life for the renaturation of urban indoors may be suitable for the prevention of Th2-mediated allergies in childhood.}, keywords = {MICE; DENDRITIC CELLS; ENDOTOXIN; LIPOPOLYSACCHARIDE; Allergy; RAGWEED; Th2-type airway inflammation; radiation-detoxified}, year = {2024}, eissn = {1422-0067} } @article{MTMT:32525004, title = {Diversity of carbapenem-resistant Klebsiella pneumoniae ST14 and emergence of a subgroup with KL64 capsular locus in the Arabian Peninsula}, url = {https://m2.mtmt.hu/api/publication/32525004}, author = {Mouftah, Shaimaa F and Pál, Tibor and Higgins, Paul G and Ghazawi, Akela and Idaghdour, Youssef and Alqahtani, Manaf and Omrani, Ali S and Rizvi, Tahir A and Sonnevend, Ágnes}, doi = {10.1007/s10096-021-04384-2}, journal-iso = {EUR J CLIN MICROBIOL}, journal = {EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES}, unique-id = {32525004}, issn = {0934-9723}, abstract = {To understand the reasons of successful spread of carbapenem-resistant Klebsiella pneumoniae ST14 (CRKP-ST14) in countries of the Arabian Peninsula, the resistome, capsular locus, carbapenemase carrying plasmid types, and core genome of isolates from the region were compared to global isolates. Thirty-nine CRKP-ST14 strains isolated from 13 hospitals in the United Arab Emirates, Bahrain, and Saudi Arabia were selected for whole genome sequencing on Illumina MiSeq platform based on the variety of carbapenemase genes carried and plasmids bearing these genes. Their resistome, capsular locus, and core genome MLST were compared to 173 CRKP-ST14 genomes available in public databases. The selected 39 CRKP-ST14 produced either NDM-1, OXA-48, OXA-162, OXA-232, KPC-2, or co-produced NDM-1 and an OXA-48-like carbapenemase. cgMLST revealed three clusters: 16 isolates from five UAE cities (C1), 11 isolates from three UAE cities and Bahrain (C2), and 5 isolates from Saudi Arabia (C3), respectively, and seven singletons. Resistance gene profile, carbapenemase genes, and their plasmid types were variable in both C1 and C2 clusters. The majority of CRKP-ST14 had KL2, but members of the C2 cluster and two further singletons possessed KL64 capsular locus. Based on cgMLST comparison of regional and global isolates, CRKP-ST14 with KL64 from four continents formed a distinct cluster, suggesting a recent emergence and spread of this variant. Our findings confirmed clonal transmission coupled with likely horizontal gene transfer in carbapenem-resistant Klebsiella pneumoniae ST14. Dissemination of this genetically flexible, highly resistant clone warrants further monitoring.}, keywords = {klebsiella pneumoniae; high-risk clone; carbapenem resistance; Capsular switch}, year = {2024}, eissn = {1435-4373} }