@article{MTMT:34790193,
	title = {Lectin-Based Immunophenotyping and Whole Proteomic Profiling of CT-26 Colon Carcinoma Murine Model.},
	url = {https://m2.mtmt.hu/api/publication/34790193},
	author = {Faragó, Anna and Zvara, Ágnes and Tiszlavicz, László and Hunyadi-Gulyás Éva, Csilla and Darula, Zsuzsanna and Hegedűs, Zoltán and Szabó, Enikő and Surguta, Sára Eszter and Tóvári, József and Puskás, László and Szebeni, Gábor},
	doi = {10.3390/ijms25074022},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34790193},
	issn = {1661-6596},
	abstract = {A murine colorectal carcinoma (CRC) model was established. CT26 colon carcinoma cells were injected into BALB/c mice's spleen to study the primary tumor and the mechanisms of cell spread of colon cancer to the liver. The CRC was verified by the immunohistochemistry of Pan Cytokeratin and Vimentin expression. Immunophenotyping of leukocytes isolated from CRC-bearing BALB/c mice or healthy controls, such as CD19+ B cells, CD11+ myeloid cells, and CD3+ T cells, was carried out using fluorochrome-labeled lectins. The binding of six lectins to white blood cells, such as galectin-1 (Gal1), siglec-1 (Sig1), Sambucus nigra lectin (SNA), Aleuria aurantia lectin (AAL), Phytolacca americana lectin (PWM), and galectin-3 (Gal3), was assayed. Flow cytometric analysis of the splenocytes revealed the increased binding of SNA, and AAL to CD3 + T cells and CD11b myeloid cells; and increased siglec-1 and AAL binding to CD19 B cells of the tumor-bearing mice. The whole proteomic analysis of the established CRC-bearing liver and spleen versus healthy tissues identified differentially expressed proteins, characteristic of the primary or secondary CRC tissues. KEGG Gene Ontology bioinformatic analysis delineated the established murine CRC characteristic protein interaction networks, biological pathways, and cellular processes involved in CRC. Galectin-1 and S100A4 were identified as upregulated proteins in the primary and secondary CT26 tumor tissues, and these were previously reported to contribute to the poor prognosis of CRC patients. Modelling the development of liver colonization of CRC by the injection of CT26 cells into the spleen may facilitate the understanding of carcinogenesis in human CRC and contribute to the development of novel therapeutic strategies.},
	keywords = {colorectal carcinoma; lectin binding sugar code; proteomic analysis of murine CRC},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Tóvári, József/0000-0002-5543-3204; Szebeni, Gábor/0000-0002-6998-5632}
}

@article{MTMT:34775247,
	title = {Exploring the Chemical Profile, In Vitro Antioxidant and Anti-Inflammatory Activities of Santolina rosmarinifolia Extracts},
	url = {https://m2.mtmt.hu/api/publication/34775247},
	author = {Schmidt, János and Juhász, Kata and Bóna, Ágnes},
	doi = {10.3390/molecules29071515},
	journal-iso = {MOLECULES},
	journal = {MOLECULES},
	volume = {29},
	unique-id = {34775247},
	issn = {1420-3049},
	abstract = {In this study, the phytochemical composition, in vitro antioxidant, and anti-inflammatory effects of the aqueous and 60% ethanolic (EtOH) extracts of Santolina rosmarinifolia leaf, flower, and root were examined. The antioxidant activity of S. rosmarinifolia extracts was determined by 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. The total phenolic content (TPC) of the extracts was measured by the Folin–Ciocalteu assay. The anti-inflammatory effect of the extracts was monitored by the Griess assay. The chemical composition of S. rosmarinifolia extracts was analysed using the LC-MS technique. According to our findings, 60% EtOH leaf extracts showed the highest Trolox equivalent antioxidant capacity (TEAC) values in both ABTS (8.39 ± 0.43 µM) and DPPH (6.71 ± 0.03 µM) antioxidant activity assays. The TPC values of the samples were in good correspondence with the antioxidant activity measurements and showed the highest gallic acid equivalent value (130.17 ± 0.01 µg/mL) in 60% EtOH leaf extracts. In addition, the 60% EtOH extracts of the leaves were revealed to possess the highest anti-inflammatory effect. The LC-MS analysis of S. rosmarinifolia extracts proved the presence of ascorbic acid, catalpol, chrysin, epigallocatechin, geraniol, isoquercitrin, and theanine, among others, for the first time. However, additional studies are needed to investigate the direct relationship between the chemical composition and physiological effects of the herb. The 60% EtOH extracts of S. rosmarinifolia leaves are potential new sources of natural antioxidants and anti-inflammatory molecules in the production of novel nutraceutical products.},
	year = {2024},
	eissn = {1420-3049}
}

@article{MTMT:34753763,
	title = {An Alternative Application of Magnetic-Activated Cell Sorting: CD45 and CD235a Based Purification of Semen and Testicular Tissue Samples},
	url = {https://m2.mtmt.hu/api/publication/34753763},
	author = {Czétány, Péter and Balló, András and Márk, László and Török, Attila and Szántó, Árpád László and Máté, Gábor},
	doi = {10.3390/ijms25073627},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34753763},
	issn = {1661-6596},
	abstract = {Magnetic activated cell sorting (MACS) is a well-known sperm selection technique, which is able to remove apoptotic spermatozoa from semen samples using the classic annexinV based method. Leukocytes and erythrocytes in semen samples or in testicular tissue processed for in vitro fertilization (IVF) could exert detrimental effects on sperm. In the current study, we rethought the aforementioned technique and used magnetic microbeads conjugated with anti-CD45/CD235a antibodies to eliminate contaminating leukocytes and erythrocytes from leukocytospermic semen samples and testicular tissue samples gained via testicular sperm extraction (TESE). With this technique, a 15.7- and a 30.8-fold reduction could be achieved in the ratio of leukocytes in semen and in the number of erythrocytes in TESE samples, respectively. Our results show that MACS is a method worth to reconsider, with more potential alternative applications. Investigations to find molecules labeling high-quality sperm population and the development of positive selection procedures based on these might be a direction of future research.},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Márk, László/0000-0002-9301-8159}
}

@article{MTMT:34745300,
	title = {Antibiotics and probiotics-induced effects on the total fatty acid composition of feces in a rat model},
	url = {https://m2.mtmt.hu/api/publication/34745300},
	author = {Marosvölgyi, Tamás and Mintál, Kitti and Borbásné Farkas, Kornélia and Sipos, Zoltán and Makszin, Lilla and Szabó, Éva and Tóth, Attila and Kocsis, Béla and Kovács, Krisztina and Hormay, Edina and Lénárd, László and Karádi, Zoltán György and Bufa, Anita},
	doi = {10.1038/s41598-024-57046-6},
	journal-iso = {SCI REP},
	journal = {SCIENTIFIC REPORTS},
	volume = {14},
	unique-id = {34745300},
	issn = {2045-2322},
	abstract = {Fatty acids (FAs) play important roles as membrane components and signal transduction molecules. Changes in short chain FA (SCFA) composition are associated with gut microbiota modifications. However, the effect of bacteria-driven changes on the detailed FA spectrum has not been explored yet. We investigated the effect of antibiotics (ABx) and/or probiotics, in four treatment groups on rat stool FA composition. Principal component analysis indicated that the chromatogram profiles of the treatment groups differ, which was also observed at different time points. Linear mixed effects models showed that in the parameters compared (sampling times, treatments. and their interactions), both the weight percentage and the concentration of FAs were affected by ABx and probiotic administration. This study found that the gut microbiome defines trans and branched saturated FAs, most saturated FAs, and unsaturated FAs with less carbon atoms. These results are among the first ones to demonstrate the restoring effects of a probiotic mixture on a substantial part of the altered total FA spectrum, and also revealed a previously unknown relationship between gut bacteria and a larger group of FAs. These findings suggest that intestinal bacteria produce not only SCFAs but also other FAs that may affect the host’s physiological processes.},
	year = {2024},
	eissn = {2045-2322},
	orcid-numbers = {Marosvölgyi, Tamás/0000-0002-4244-5513; Mintál, Kitti/0009-0003-4739-2902; Borbásné Farkas, Kornélia/0000-0002-5349-6527; Sipos, Zoltán/0000-0001-7845-8116; Makszin, Lilla/0000-0002-9764-4763; Hormay, Edina/0009-0004-0598-5061}
}

@article{MTMT:34724787,
	title = {Trans isomeric fatty acids in human milk and their role in infant health and development},
	url = {https://m2.mtmt.hu/api/publication/34724787},
	author = {Hatem, Okba and Kaçar, Ömer Furkan and Kaçar, Hüsna Kaya and Szentpéteri, József László (Joe Petersburger) and Marosvölgyi, Tamás and Szabó, Éva},
	doi = {10.3389/fnut.2024.1379772},
	journal-iso = {FRONT NUTR},
	journal = {FRONTIERS IN NUTRITION},
	volume = {11},
	unique-id = {34724787},
	issn = {2296-861X},
	abstract = {It is well known that long chain polyunsaturated fatty acids (LCPUFAs) play an important role in neurodevelopment in the perinatal life. The most important source of these fatty acids is the diet, however, they can also be formed in the human body from their shorter chain precursors, the essential fatty acids. Since the WHO recommends exclusive breastfeeding for the first six months after birth, the exclusive source of these fatty acids for breastfed infants is human milk, which can be influenced by the mother’s diet. Unsaturated fatty acids can have either cis or trans configuration double bond in their chain with distinct physiological effects. Cis isomeric unsaturated fatty acids have several beneficial effects, while trans isomers are mostly detrimental, because of their similar structure to saturated fatty acids. Trans fatty acids (TFAs) can be further subdivided into industrial (iTFA) and ruminant-derived trans fatty acids (rTFA). However, the physiological effects of these two TFA subgroups may differ. In adults, dietary intake of iTFA has been linked to atherosclerosis, insulin resistance, obesity, chronic inflammation, and increased development of certain cancers, among other diseases. However, iTFAs can have a negative impact on health not only in adulthood but in childhood too. Results from previous studies have shown that iTFAs have a significant negative effect on LCPUFA levels in the blood of newborns and infants. In addition, iTFAs can affect the growth and development of infants, and animal studies suggest that they might even have lasting negative effects later in life. Since the only source of TFAs in the human body is the diet, the TFA content of breast milk may determine the TFA supply of breastfed infants and thus affect the levels of LCPUFAs important for neurodevelopment and the health of infants. In this review, we aim to provide an overview of the TFA content in human milk available in the literature and their potential effects on infant health and development.},
	year = {2024},
	eissn = {2296-861X},
	orcid-numbers = {Marosvölgyi, Tamás/0000-0002-4244-5513}
}

@article{MTMT:34723100,
	title = {Comprehensive within-population characterization of the Transylvanian Seklers based on high-resolution autosomal marker data},
	url = {https://m2.mtmt.hu/api/publication/34723100},
	author = {Bánfai, Zsolt and Adam, Valerian and Sümegi, Katalin and Szabo, Andras and Büki, Gergely and Magyari, Lili and Hadzsiev, Kinga and Kasler, Miklos and Melegh, Bela},
	journal-iso = {EUR J HUM GENET},
	journal = {EUROPEAN JOURNAL OF HUMAN GENETICS},
	volume = {32},
	unique-id = {34723100},
	issn = {1018-4813},
	keywords = {Biochemistry & Molecular Biology},
	year = {2024},
	eissn = {1476-5438},
	pages = {313-313}
}

@article{MTMT:34684525,
	title = {Integrative Epigenetic and Molecular Analysis Reveals a Novel Promoter for a New Isoform of the Transcription Factor TEAD4},
	url = {https://m2.mtmt.hu/api/publication/34684525},
	author = {Rashidiani, Shima and Mamo, Gizaw and Farkas, Benjámin and Szabadi, András and Farkas, Bálint and Uszkai, Veronika and Császár, András and Brandt, Barbara and Kovács, Kálmán András and Pap, Marianna and Rauch, Tibor},
	doi = {10.3390/ijms25042223},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34684525},
	issn = {1661-6596},
	abstract = {TEAD4 is a transcription factor that plays a crucial role in the Hippo pathway by regulating the expression of genes related to proliferation and apoptosis. It is also involved in the maintenance and differentiation of the trophectoderm during pre- and post-implantation embryonic development. An alternative promoter for the TEAD4 gene was identified through epigenetic profile analysis, and a new transcript from the intronic region of TEAD4 was discovered using the 5'RACE method. The transcript of the novel promoter encodes a TEAD4 isoform (TEAD4-ΔN) that lacks the DNA-binding domain but retains the C-terminal protein-protein interaction domain. Gene expression studies, including end-point PCR and Western blotting, showed that full-length TEAD4 was present in all investigated tissues. However, TEAD4-ΔN was only detectable in certain cell types. The TEAD4-ΔN promoter is conserved throughout evolution and demonstrates transcriptional activity in transient-expression experiments. Our study reveals that TEAD4 interacts with the alternative promoter and increases the expression of the truncated isoform. DNA methylation plays a crucial function in the restricted expression of the TEAD4-ΔN isoform in specific tissues, including the umbilical cord and the placenta. The data presented indicate that the DNA-methylation status of the TEAD4-ΔN promoter plays a critical role in regulating organ size, cancer development, and placenta differentiation.},
	keywords = {DNA methylation; TRANSCRIPTIONAL REGULATION; TEAD4; alternative promoter; Hippo/TEAD signaling},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Farkas, Bálint/0000-0003-0638-2057}
}

@CONFERENCE{MTMT:34667054,
	title = {Terhességi cukorbetegekben és egészséges várandósokban jellemző zsírsavak változásai},
	url = {https://m2.mtmt.hu/api/publication/34667054},
	author = {Frank, Eszter Anita and Pergel, Mónika and Szabó, Éva and Ungár Tamás Lászlóné Polyák, Éva},
	booktitle = {Jubileumi Szakkollégiumi Konferencia: Absztrakt kötet},
	unique-id = {34667054},
	year = {2024},
	pages = {14-14},
	orcid-numbers = {Ungár Tamás Lászlóné Polyák, Éva/0000-0002-3648-1614}
}

@article{MTMT:34541583,
	title = {Emerging Lipid Targets in Glioblastoma},
	url = {https://m2.mtmt.hu/api/publication/34541583},
	author = {Darwish, Ammar and Pammer, Milán and Gallyas, Ferenc and Vigh, László and Balogi, Zsolt and Juhász, Kata},
	doi = {10.3390/cancers16020397},
	journal-iso = {CANCERS},
	journal = {CANCERS},
	volume = {16},
	unique-id = {34541583},
	abstract = {GBM accounts for most of the fatal brain cancer cases, making it one of the deadliest tumor types. GBM is characterized by severe progression and poor prognosis with a short survival upon conventional chemo- and radiotherapy. In order to improve therapeutic efficiency, considerable efforts have been made to target various features of GBM. One of the targetable features of GBM is the rewired lipid metabolism that contributes to the tumor's aggressive growth and penetration into the surrounding brain tissue. Lipid reprogramming allows GBM to acquire survival, proliferation, and invasion benefits as well as supportive modulation of the tumor microenvironment. Several attempts have been made to find novel therapeutic approaches by exploiting the lipid metabolic reprogramming in GBM. In recent studies, various components of de novo lipogenesis, fatty acid oxidation, lipid uptake, and prostaglandin synthesis have been considered promising targets in GBM. Emerging data also suggest a significant role hence therapeutic potential of the endocannabinoid metabolic pathway in GBM. Here we review the lipid-related GBM characteristics in detail and highlight specific targets with their potential therapeutic use in novel antitumor approaches.},
	keywords = {GBM; lipid therapy; tumor hallmarks; tumor lipid},
	year = {2024},
	eissn = {2072-6694},
	orcid-numbers = {Gallyas, Ferenc/0000-0002-1906-4333}
}

@article{MTMT:34486045,
	title = {Follicular Fluid Proteomic Analysis of Women Undergoing Assisted Reproduction Suggests That Apolipoprotein A1 Is a Potential Fertility Marker},
	url = {https://m2.mtmt.hu/api/publication/34486045},
	author = {Kurdi, Csilla and Schmidt, János and Horváth-Szalai, Zoltán and Mauchart, Péter and Gödöny, Krisztina and Várnagy, Ákos and Kovács, L. Gábor and Kőszegi, Tamás},
	doi = {10.3390/ijms25010486},
	journal-iso = {INT J MOL SCI},
	journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES},
	volume = {25},
	unique-id = {34486045},
	issn = {1661-6596},
	abstract = {Infertility affects millions worldwide, posing a significant global health challenge. The proteomic analysis of follicular fluid provides a comprehensive view of the complex molecular landscape within ovarian follicles, offering valuable information on the factors influencing oocyte development and on the overall reproductive health. The follicular fluid is derived from the plasma and contains various proteins that can have different roles in oocyte health and infertility, and this fluid is a critical microenvironment for the developing oocytes as well. Using the high-performance liquid chromatography-mass spectrometry method, we investigated the protein composition of the follicular fluid, and after classification, we carried out relative quantification of the identified proteins in the pregnant (P) and non-pregnant (NP) groups. Based on the protein–protein interaction analysis, albumin and apolipoprotein A1 (ApoA1) were found to be hub proteins, and the quantitative comparison of the P and NP groups resulted in a significantly lower concentration of ApoA1 and high-density lipoprotein cholesterol in the P group. As both molecules are involved in the cholesterol transport, we also investigated their role in the development of oocytes and in the prediction of fertility.},
	keywords = {PREDICTIVE VALUE; Mass spectrometry; follicular fluid; proteomics; apolipoprotein A1; HDL CHOLESTEROL; assisted reproductive treatment},
	year = {2024},
	eissn = {1422-0067},
	orcid-numbers = {Kovács, L. Gábor/0000-0001-5298-5401}
}