TY - JOUR AU - Bodai, László AU - Zsindely, Nóra AU - Molnár-Gáspár, Renáta AU - Kristó, Ildikó AU - Komonyi, Orbán AU - Boros, Imre Miklós TI - Ecdysone Induced Gene Expression Is Associated with Acetylation of Histone H3 Lysine 23 in Drosophila melanogaster JF - PLOS ONE J2 - PLOS ONE VL - 7 PY - 2012 IS - 7 PG - 10 SN - 1932-6203 DO - 10.1371/journal.pone.0040565 UR - https://m2.mtmt.hu/api/publication/2052334 ID - 2052334 N1 - Cited By :12 Export Date: 31 August 2019 Correspondence Address: Bodai, L.; Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary; email: lbodai@yahoo.com Chemicals/CAS: Acetyltransferases, 2.3.1.-; DNA-Binding Proteins; Drosophila Proteins; Ecdysone, 3604-87-3; Eip74EF protein, Drosophila; Eip75B protein, Drosophila, 126968-14-7; Histones; Lysine, 56-87-1; Transcription Factors; nejire protein, Drosophila; p300-CBP Transcription Factors, 2.3.1.48 Cited By :12 Export Date: 2 September 2019 Correspondence Address: Bodai, L.; Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary; email: lbodai@yahoo.com Chemicals/CAS: Acetyltransferases, 2.3.1.-; DNA-Binding Proteins; Drosophila Proteins; Ecdysone, 3604-87-3; Eip74EF protein, Drosophila; Eip75B protein, Drosophila, 126968-14-7; Histones; Lysine, 56-87-1; Transcription Factors; nejire protein, Drosophila; p300-CBP Transcription Factors, 2.3.1.48 Cited By :14 Export Date: 12 February 2020 Correspondence Address: Bodai, L.; Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary; email: lbodai@yahoo.com Chemicals/CAS: Acetyltransferases, 2.3.1.-; DNA-Binding Proteins; Drosophila Proteins; Ecdysone, 3604-87-3; Eip74EF protein, Drosophila; Eip75B protein, Drosophila, 126968-14-7; Histones; Lysine, 56-87-1; Transcription Factors; nejire protein, Drosophila; p300-CBP Transcription Factors, 2.3.1.48 Funding Agency and Grant Number: Hungarian National Science Foundation (OTKA)Orszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [PD-72491, 77443] Funding text: This work was supported by the Hungarian National Science Foundation (OTKA, http://www.otka.hu) grant PD-72491 to Dr. Bodai and grant 77443 to Dr. Boros. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Cited By :15 Export Date: 4 September 2020 Correspondence Address: Bodai, L.; Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary; email: lbodai@yahoo.com Chemicals/CAS: Acetyltransferases, 2.3.1.-; DNA-Binding Proteins; Drosophila Proteins; Ecdysone, 3604-87-3; Eip74EF protein, Drosophila; Eip75B protein, Drosophila, 126968-14-7; Histones; Lysine, 56-87-1; Transcription Factors; nejire protein, Drosophila; p300-CBP Transcription Factors, 2.3.1.48 Cited By :15 Export Date: 9 September 2020 Correspondence Address: Bodai, L.; Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary; email: lbodai@yahoo.com Chemicals/CAS: Acetyltransferases, 2.3.1.-; DNA-Binding Proteins; Drosophila Proteins; Ecdysone, 3604-87-3; Eip74EF protein, Drosophila; Eip75B protein, Drosophila, 126968-14-7; Histones; Lysine, 56-87-1; Transcription Factors; nejire protein, Drosophila; p300-CBP Transcription Factors, 2.3.1.48 Cited By :18 Export Date: 17 January 2021 Correspondence Address: Bodai, L.; Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary; email: lbodai@yahoo.com Chemicals/CAS: Acetyltransferases, 2.3.1.-; DNA-Binding Proteins; Drosophila Proteins; Ecdysone, 3604-87-3; Eip74EF protein, Drosophila; Eip75B protein, Drosophila, 126968-14-7; Histones; Lysine, 56-87-1; Transcription Factors; nejire protein, Drosophila; p300-CBP Transcription Factors, 2.3.1.48 Cited By :18 Export Date: 14 March 2021 Correspondence Address: Bodai, L.; Department of Biochemistry and Molecular Biology, , Szeged, Hungary; email: lbodai@yahoo.com Chemicals/CAS: Acetyltransferases, 2.3.1.-; DNA-Binding Proteins; Drosophila Proteins; Ecdysone, 3604-87-3; Eip74EF protein, Drosophila; Eip75B protein, Drosophila, 126968-14-7; Histones; Lysine, 56-87-1; Transcription Factors; nejire protein, Drosophila; p300-CBP Transcription Factors, 2.3.1.48 AB - Posttranslational modification of histones regulates transcription but the exact role that acetylation of specific lysine residues plays in biological processes in vivo is still not clearly understood. To assess the contribution of different histone modifications to transcriptional activation in vivo, we determined the acetylation patterns on the ecdysone induced Eip74EF and Eip75B genes in Drosophila melanogaster larvae by chromatin immunoprecipitation. We found that acetylation of histone H3 lysine 23 is localized to promoters and correlates with endogenous ecdysone induced gene activation. In contrast, acetylation of lysines 8, 12 and 16 of histone H4 and lysine 9 of histone H3 showed minor differences in their distribution on the regulatory and transcribed regions tested, and had limited or no correlation with ecdysone induced transcriptional activity. We found that dCBP, which is encoded by the nejire gene, acetylates H3 lysine 23 in vivo, and silencing of nejire leads to reduced expression of the Eip74EF and Eip75B genes. Our results suggest that acetylation of specific lysine residues of histones contribute specifically to the dynamic regulation of transcription. Furthermore, along with previous studies identify CBP dependent H3 lysine 23 acetylation as an evolutionarily conserved chromatin modification involved in steroid induced gene activation. LA - English DB - MTMT ER - TY - JOUR AU - Pardi, Norbert AU - Vámos, Edith AU - Újfaludi, Zsuzsanna AU - Komonyi, Orbán AU - Bodai, László AU - Boros, Imre Miklós TI - In vivo effects of abolishing the single canonical sumoylation site in the C-terminal region of Drosophila p53 JF - ACTA BIOLOGICA HUNGARICA (1983-2018) J2 - ACTA BIOL HUNG VL - 62 PY - 2011 IS - 4 SP - 397 EP - 412 PG - 16 SN - 0236-5383 DO - 10.1556/ABiol.62.2011.4.6 UR - https://m2.mtmt.hu/api/publication/1772853 ID - 1772853 N1 - Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary Cited By :2 Export Date: 31 August 2019 CODEN: ABHUE Correspondence Address: Boros, I.; Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chemicals/CAS: SUMO-1 Protein; Tumor Suppressor Protein p53 Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary Cited By :2 Export Date: 2 September 2019 CODEN: ABHUE Correspondence Address: Boros, I.; Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chemicals/CAS: SUMO-1 Protein; Tumor Suppressor Protein p53 Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary Cited By :2 Export Date: 12 February 2020 CODEN: ABHUE Correspondence Address: Boros, I.; Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chemicals/CAS: SUMO-1 Protein; Tumor Suppressor Protein p53 Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary Cited By :2 Export Date: 9 September 2020 CODEN: ABHUE Correspondence Address: Boros, I.; Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chemicals/CAS: SUMO-1 Protein; Tumor Suppressor Protein p53 Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary Cited By :2 Export Date: 17 January 2021 CODEN: ABHUE Correspondence Address: Boros, I.; Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chemicals/CAS: SUMO-1 Protein; Tumor Suppressor Protein p53 LA - English DB - MTMT ER - TY - THES AU - Buzás-Bereczki, Orsolya TI - A TATA-kötő fehérje asszociált faktor 3 (TAF3) p53-mal való kölcsönhatásának funkcionális vizsgálata PB - Szegedi Tudományegyetem (SZTE) PY - 2010 SP - 102 UR - https://m2.mtmt.hu/api/publication/1454460 ID - 1454460 LA - Hungarian DB - MTMT ER - TY - JOUR AU - Peláez, IM AU - Kalogeropoulou, M AU - Ferraro, A AU - Voulgari, A AU - Pankotai, Tibor AU - Boros, Imre Miklós AU - Pintzas, A TI - Oncogenic RAS alters the global and gene-specific histone modification pattern during epithelial-mesenchymal transition in colorectal carcinoma cells JF - INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY J2 - INT J BIOCHEM CELL B VL - 42 PY - 2010 IS - 6 SP - 911 EP - 920 PG - 10 SN - 1357-2725 DO - 10.1016/j.biocel.2010.01.024 UR - https://m2.mtmt.hu/api/publication/1447235 ID - 1447235 LA - English DB - MTMT ER - TY - JOUR AU - Pankotai, Tibor AU - Újfaludi, Zsuzsanna AU - Vámos, Edith AU - Suri, K AU - Boros, Imre Miklós TI - The dissociable RPB4 subunit of RNA Pol II has vital functions in Drosophila JF - MOLECULAR GENETICS AND GENOMICS J2 - MOL GENET GENOMICS VL - 283 PY - 2010 IS - 1 SP - 89 EP - 97 PG - 9 SN - 1617-4615 DO - 10.1007/s00438-009-0499-6 UR - https://m2.mtmt.hu/api/publication/1447205 ID - 1447205 N1 - Megjegyzés-22192136 DI: 10.1007/s00438-009-0499-6 Megjegyzés-22436360 DI: 10.1007/s00438-009-0499-6 Megjegyzés-22192025 DI: 10.1007/s00438-009-0499-6 Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary Biological Research Center, Institute of Biochemistry, Temesvári krt. 62, 6726 Szeged, Hungary Cited By :1 Export Date: 2 September 2019 CODEN: MGGOA Correspondence Address: Boros, I. M.; Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Funding details: K77443 Funding text 1: Acknowledgments We are grateful to O. Komonyi and E. Kovács for their help at the start of this work, and L. Tora for his help in antibody production. We also thank for L. Bodai and P. Deák for critical reading the ms and comments. The technical help of Ökrösné Kati and Cs. Bakota Adrienn is greatly appreciated. Support to this work was provided by the Hungarian State Science Fund OTKA K77443 to IB. Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary Biological Research Center, Institute of Biochemistry, Temesvári krt. 62, 6726 Szeged, Hungary Cited By :1 Export Date: 12 February 2020 CODEN: MGGOA Correspondence Address: Boros, I. M.; Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Funding details: K77443 Funding text 1: Acknowledgments We are grateful to O. Komonyi and E. Kovács for their help at the start of this work, and L. Tora for his help in antibody production. We also thank for L. Bodai and P. Deák for critical reading the ms and comments. The technical help of Ökrösné Kati and Cs. Bakota Adrienn is greatly appreciated. Support to this work was provided by the Hungarian State Science Fund OTKA K77443 to IB. Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary Biological Research Center, Institute of Biochemistry, Temesvári krt. 62, 6726 Szeged, Hungary Cited By :2 Export Date: 9 September 2020 CODEN: MGGOA Correspondence Address: Boros, I. M.; Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Funding details: K77443 Funding text 1: Acknowledgments We are grateful to O. Komonyi and E. Kovács for their help at the start of this work, and L. Tora for his help in antibody production. We also thank for L. Bodai and P. Deák for critical reading the ms and comments. The technical help of Ökrösné Kati and Cs. Bakota Adrienn is greatly appreciated. Support to this work was provided by the Hungarian State Science Fund OTKA K77443 to IB. Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary Biological Research Center, Institute of Biochemistry, Temesvári krt. 62, 6726 Szeged, Hungary Cited By :2 Export Date: 17 January 2021 CODEN: MGGOA Correspondence Address: Boros, I. M.; Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Funding details: K77443 Funding text 1: Acknowledgments We are grateful to O. Komonyi and E. Kovács for their help at the start of this work, and L. Tora for his help in antibody production. We also thank for L. Bodai and P. Deák for critical reading the ms and comments. The technical help of Ökrösné Kati and Cs. Bakota Adrienn is greatly appreciated. Support to this work was provided by the Hungarian State Science Fund OTKA K77443 to IB. LA - English DB - MTMT ER - TY - JOUR AU - Pankotai, Tibor AU - Popescu, C AU - Martin, D AU - Grau, B AU - Zsindely, Nóra AU - Bodai, László AU - Tora, L AU - Ferrus, A AU - Boros, Imre Miklós TI - Genes of the Ecdysone Biosynthesis Pathway Are Regulated by the dATAC Histone Acetyltransferase Complex in Drosophila JF - MOLECULAR AND CELLULAR BIOLOGY J2 - MOL CELL BIOL VL - 30 PY - 2010 IS - 17 SP - 4254 EP - 4266 PG - 13 SN - 0270-7306 DO - 10.1128/MCB.00142-10 UR - https://m2.mtmt.hu/api/publication/1436491 ID - 1436491 N1 - Megjegyzés-22204228 DI: 10.1128/MCB.00142-10 Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary Institute of Evolutionary Biology (CSIC-UPF), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CU de Strasbourg, BP 10142, 67404 Illkirch Cedex, France Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary Banat University of Agricultural Sciences, Department of Genetics, Aradului Street 119, Timisoara 300126, Romania Cited By :19 Export Date: 31 August 2019 CODEN: MCEBD Correspondence Address: Ferrús, A.; Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain; email: aferrus@cajal.csic.es Chemicals/CAS: histone acetyltransferase, 9054-51-7; cholesterol, 57-88-5; ADA2 protein, Drosophila, 2.3.1.48; Cholesterol, 57-88-5; Drosophila Proteins; Ecdysone, 3604-87-3; Histone Acetyltransferases, 2.3.1.48; Protein Subunits; dik protein, Drosophila, 2.3.1.48 Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary Institute of Evolutionary Biology (CSIC-UPF), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CU de Strasbourg, BP 10142, 67404 Illkirch Cedex, France Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary Banat University of Agricultural Sciences, Department of Genetics, Aradului Street 119, Timisoara 300126, Romania Cited By :19 Export Date: 2 September 2019 CODEN: MCEBD Correspondence Address: Ferrús, A.; Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain; email: aferrus@cajal.csic.es Chemicals/CAS: histone acetyltransferase, 9054-51-7; cholesterol, 57-88-5; ADA2 protein, Drosophila, 2.3.1.48; Cholesterol, 57-88-5; Drosophila Proteins; Ecdysone, 3604-87-3; Histone Acetyltransferases, 2.3.1.48; Protein Subunits; dik protein, Drosophila, 2.3.1.48 Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary Institute of Evolutionary Biology (CSIC-UPF), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CU de Strasbourg, BP 10142, 67404 Illkirch Cedex, France Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary Banat University of Agricultural Sciences, Department of Genetics, Aradului Street 119, Timisoara 300126, Romania Cited By :19 Export Date: 12 February 2020 CODEN: MCEBD Correspondence Address: Ferrús, A.; Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain; email: aferrus@cajal.csic.es Chemicals/CAS: histone acetyltransferase, 9054-51-7; cholesterol, 57-88-5; ADA2 protein, Drosophila, 2.3.1.48; Cholesterol, 57-88-5; Drosophila Proteins; Ecdysone, 3604-87-3; Histone Acetyltransferases, 2.3.1.48; Protein Subunits; dik protein, Drosophila, 2.3.1.48 Funding Agency and Grant Number: Spain and Hungary [HH2006-0025]; Hungary and France [FR-33/08]; Hungarian State Science Fund [OTKA PD72491, OTKA K77443]; FRMFondation pour la Recherche Medicale; European CommunityEuropean Community (EC) [HPRN-CT 00504228, STREP LSHG-CT-2004-502950, EUTRACC LSHG-CT-2007-037445]; Reseau National des Genopoles [260]; INCAInstitut National du Cancer (INCA) France [2008-UBICAN]; Spanish Ministry of Research [BFU2006-10180]; TAF-CHROMATIN European Network [MRTN-CT-2004-504288] Funding text: Binational grants between Spain and Hungary (HH2006-0025) and Hungary and France (FR-33/08) provided support to B.G., L.T., and I.B.L.B. was supported by the Hungarian State Science Fund (OTKA PD72491). Research was supported by funds from the FRM and the European Community (HPRN-CT 00504228, STREP LSHG-CT-2004-502950, and EUTRACC LSHG-CT-2007-037445) and Reseau National des Genopoles (no. 260) and by INCA (2008-UBICAN) grants to L.T.; Spanish Ministry of Research BFU2006-10180 and TAF-CHROMATIN European Network MRTN-CT-2004-504288 grants to A.F. and I.B.; and the Hungarian State Science Fund (OTKA K77443) to I.B. Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary Institute of Evolutionary Biology (CSIC-UPF), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CU de Strasbourg, BP 10142, 67404 Illkirch Cedex, France Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary Banat University of Agricultural Sciences, Department of Genetics, Aradului Street 119, Timisoara 300126, Romania Cited By :21 Export Date: 4 September 2020 CODEN: MCEBD Correspondence Address: Ferrús, A.; Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain; email: aferrus@cajal.csic.es Chemicals/CAS: histone acetyltransferase, 9054-51-7; cholesterol, 57-88-5; ADA2 protein, Drosophila, 2.3.1.48; Cholesterol, 57-88-5; Drosophila Proteins; Ecdysone, 3604-87-3; Histone Acetyltransferases, 2.3.1.48; Protein Subunits; dik protein, Drosophila, 2.3.1.48 Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary Institute of Evolutionary Biology (CSIC-UPF), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CU de Strasbourg, BP 10142, 67404 Illkirch Cedex, France Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary Banat University of Agricultural Sciences, Department of Genetics, Aradului Street 119, Timisoara 300126, Romania Cited By :21 Export Date: 9 September 2020 CODEN: MCEBD Correspondence Address: Ferrús, A.; Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain; email: aferrus@cajal.csic.es Chemicals/CAS: histone acetyltransferase, 9054-51-7; cholesterol, 57-88-5; ADA2 protein, Drosophila, 2.3.1.48; Cholesterol, 57-88-5; Drosophila Proteins; Ecdysone, 3604-87-3; Histone Acetyltransferases, 2.3.1.48; Protein Subunits; dik protein, Drosophila, 2.3.1.48 Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary Institute of Evolutionary Biology (CSIC-UPF), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CU de Strasbourg, BP 10142, 67404 Illkirch Cedex, France Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary Banat University of Agricultural Sciences, Department of Genetics, Aradului Street 119, Timisoara 300126, Romania Cited By :21 Export Date: 17 January 2021 CODEN: MCEBD Correspondence Address: Ferrús, A.; Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain; email: aferrus@cajal.csic.es Chemicals/CAS: histone acetyltransferase, 9054-51-7; cholesterol, 57-88-5; ADA2 protein, Drosophila, 2.3.1.48; Cholesterol, 57-88-5; Drosophila Proteins; Ecdysone, 3604-87-3; Histone Acetyltransferases, 2.3.1.48; Protein Subunits; dik protein, Drosophila, 2.3.1.48 Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary Institute of Evolutionary Biology (CSIC-UPF), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, CU de Strasbourg, BP 10142, 67404 Illkirch Cedex, France Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, H-6726 Szeged, Hungary Banat University of Agricultural Sciences, Department of Genetics, Aradului Street 119, Timisoara 300126, Romania Cited By :21 Export Date: 14 March 2021 CODEN: MCEBD Correspondence Address: Ferrús, A.; Cajal Institute (CSIC), Ave. Dr. Arce 37, 28002 Madrid, Spain; email: aferrus@cajal.csic.es Chemicals/CAS: histone acetyltransferase, 9054-51-7; cholesterol, 57-88-5; ADA2 protein, Drosophila, 2.3.1.48; Cholesterol, 57-88-5; Drosophila Proteins; Ecdysone, 3604-87-3; Histone Acetyltransferases, 2.3.1.48; Protein Subunits; dik protein, Drosophila, 2.3.1.48 AB - Uncovering mechanisms that regulate ecdysone production is an important step toward understanding the regulation of insect metamorphosis and processes in steroid-related pathologies. We report here the transcriptome analysis of Drosophila melanogaster dAda2a and dAda3 mutants, in which subunits of the ATAC acetyltransferase complex are affected. In agreement with the fact that these mutations lead to lethality at the start of metamorphosis, both the ecdysone levels and the ecdysone receptor binding to polytene chromosomes are reduced in these flies. The cytochrome genes (spookier, phantom, disembodied, and shadow) involved in steroid conversion in the ring gland are downregulated, while the gene shade, which is involved in converting ecdysone into its active form in the periphery, is upregulated in these dATAC subunit mutants. Moreover, driven expression of dAda3 at the site of ecdysone synthesis partially rescues dAda3 mutants. Mutants of dAda2b, a subunit of the dSAGA histone acetyltransferase complex, do not share phenotype characteristics and RNA profile alterations with dAda2a mutants, indicating that the ecdysone biosynthesis genes are regulated by dATAC, but not by dSAGA. Thus, we provide one of the first examples of the coordinated regulation of a functionally linked set of genes by the metazoan-specific ATAC complex. LA - English DB - MTMT ER - TY - JOUR AU - Komonyi, Orbán AU - Schauer, T AU - Pápai, Gábor AU - Deák, Péter AU - Boros, Imre Miklós TI - A product of the bicistronic Drosophila melanogaster gene CG31241, which also encodes a trimethylguanosine synthase, plays a role in telomere protection. [Short Report] TS - [Short Report] JF - JOURNAL OF CELL SCIENCE J2 - J CELL SCI VL - 122 PY - 2009 IS - 6 SP - 769 EP - 774 PG - 6 SN - 0021-9533 DO - 10.1242/jcs.035097 UR - https://m2.mtmt.hu/api/publication/1454634 ID - 1454634 N1 - Szövegében 3 oldalnál hosszabb Short Report, ezért besorolása szakcikk az MTA V. Osztályának ajánlása alapján (csonkap5, SZTE admin5) Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közp̧ fasor 52, H-6726 Szeged, Hungary Institute of Biochemistry, Biological Research Center, Temesvari krt.62, H-6726 Szeged, Hungary Cited By :7 Export Date: 12 February 2020 CODEN: JNCSA Correspondence Address: Boros, I.M.; Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közp̧ fasor 52, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közp̧ fasor 52, H-6726 Szeged, Hungary Institute of Biochemistry, Biological Research Center, Temesvari krt.62, H-6726 Szeged, Hungary Cited By :8 Export Date: 9 September 2020 CODEN: JNCSA Correspondence Address: Boros, I.M.; Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közp̧ fasor 52, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közp̧ fasor 52, H-6726 Szeged, Hungary Institute of Biochemistry, Biological Research Center, Temesvari krt.62, H-6726 Szeged, Hungary Cited By :9 Export Date: 17 January 2021 CODEN: JNCSA Correspondence Address: Boros, I.M.; Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közp̧ fasor 52, H-6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu AB - Although telomere formation occurs through a different mechanism in Drosophila compared with other organisms, telomere associations result from mutations in homologous genes, indicating the involvement of similar pathways in chromosome end protection. We report here that mutations of the Drosophila melanogaster gene CG31241 lead to high frequency chromosome end fusions. CG31241 is a bicistronic gene that encodes trimethylguanosine synthase (TGS1), which forms the m3G caps of noncoding small RNAs, and a novel protein, DTL. We show that although TGS1 has no role in telomere protection, DTL is localized at specific sites, including the ends of polytene chromosomes, and its loss results in telomere associations. Mutations of ATM- and Rad3-related (ATR) kinase suppress telomere fusions in the absence of DTL. Thus, genetic interactions place DTL in an ATR-related pathway in telomere protection. In contrast to ATR kinase, mutations of ATM (ataxia telangiectasia mutated) kinase, which acts in a partially overlapping pathway of telomere protection, do not suppress formation of telomere associations in the absence of DTL. Thus, uncovering the role of DTL will help to dissect the evolutionary conserved pathway(s) controlling ATM-ATR-related telomere protection. LA - English DB - MTMT ER - TY - JOUR AU - Zsindely, Nóra AU - Pankotai, Tibor AU - Újfaludi, Zsuzsanna AU - Lakatos, D AU - Komonyi, Orbán AU - Bodai, László AU - Tora, László AU - Boros, Imre Miklós TI - The loss of histone H3 lysine 9 acetylation due to dSAGA-specific dAda2b mutation influences the expression of only a small subset of genes JF - NUCLEIC ACIDS RESEARCH J2 - NUCLEIC ACIDS RES VL - 37 PY - 2009 IS - 20 SP - 6665 EP - 6680 PG - 16 SN - 0305-1048 DO - 10.1093/nar/gkp722 UR - https://m2.mtmt.hu/api/publication/1264348 ID - 1264348 AB - In Drosophila, the dADA2b-containing dSAGA complex is involved in histone H3 lysine 9 and 14 acetylation. Curiously, although the lysine 9- and 14-acetylated histone H3 levels are drastically reduced in dAda2b mutants, these animals survive until a late developmental stage. To study the molecular consequences of the loss of histone H3 lysine 9 and 14 acetylation, we compared the total messenger ribonucleic acid (mRNA) profiles of wild type and dAda2b mutant animals at two developmental stages. Global gene expression profiling indicates that the loss of dSAGA-specific H3 lysine 9 and 14 acetylation results in the expression change (up- or down-regulation) of a rather small subset of genes and does not cause a general transcription de-regulation. Among the genes up-regulated in dAda2b mutants, particularly high numbers are those which play roles in antimicrobial defense mechanisms. Results of chromatin immunoprecipitation experiments indicate that in dAda2b mutants, the lysine 9-acetylated histone H3 levels are decreased both at dSAGA up- and down-regulated genes. In contrast to that, in the promoters of dSAGA-independent ribosomal protein genes a high level of histone H3K9ac is maintained in dAda2b mutants. Our data suggest that by acetylating H3 at lysine 9, dSAGA modifies Pol II accessibility to specific promoters differently. LA - English DB - MTMT ER - TY - JOUR AU - Boros, Imre Miklós TI - Histone modifying complexes of Drosophila and their role in nuclear hormone mediated gene expression regulation JF - ACTA PHYSIOLOGICA HUNGARICA J2 - ACTA PHYSIOL HUNG VL - 96 PY - 2009 IS - 1 SP - 60 EP - 61 PG - 2 SN - 0231-424X UR - https://m2.mtmt.hu/api/publication/247826 ID - 247826 LA - English DB - MTMT ER - TY - JOUR AU - Ciurciu, Anita AU - Tombácz, István AU - Popescu, Christina AU - Boros, Imre Miklós TI - GAL4 induces transcriptionally active puff in the absence of dSAGA- and ATAC-specific chromatin acetylation in the Drosophila melanogaster polytene chromosome JF - CHROMOSOMA J2 - CHROMOSOMA VL - 118 PY - 2009 IS - 4 SP - 513 EP - 526 PG - 14 SN - 0009-5915 DO - 10.1007/s00412-009-0215-7 UR - https://m2.mtmt.hu/api/publication/247825 ID - 247825 N1 - Megjegyzés-20786963 FU: Hungarian Science Fund [T046414, K77443]; Hungarian Ministry of Health : [ETT 078/2007]; EU [LSHG-CT-2004-502950]; European Community : [HPRN-CT-2004-504228] FX: We are grateful to Laszlo Tora, Alberto Ferrus, Sonja Georgieva, and : Kristen Johansen for kindly providing us Pol II, dADA3, dGCN5, and : JIL-1 antibodies. This work was supported by grants from the Hungarian : Science Fund (OTKA T046414 and K77443), the Hungarian Ministry of : Health (ETT 078/2007), and EU FP-6 (LSHG-CT-2004-502950). A. C. and C. : P. are European Community RTN Marie Curie research fellows supported by : the grant HPRN-CT-2004-504228. Megjegyzés-20787951 FU: Hungarian Science Fund [T046414, K77443]; Hungarian Ministry of Health : [ETT 078/2007]; EU [LSHG-CT-2004-502950]; European Community : [HPRN-CT-2004-504228] FX: We are grateful to Laszlo Tora, Alberto Ferrus, Sonja Georgieva, and : Kristen Johansen for kindly providing us Pol II, dADA3, dGCN5, and : JIL-1 antibodies. This work was supported by grants from the Hungarian : Science Fund (OTKA T046414 and K77443), the Hungarian Ministry of : Health (ETT 078/2007), and EU FP-6 (LSHG-CT-2004-502950). A. C. and C. : P. are European Community RTN Marie Curie research fellows supported by : the grant HPRN-CT-2004-504228. Megjegyzés-22192143 DI: 10.1007/s00412-009-0215-7 Megjegyzés-22204235 DI: 10.1007/s00412-009-0215-7 Megjegyzés-22207401 DI: 10.1007/s00412-009-0215-7 Megjegyzés-20784367 FU: Hungarian Science Fund [T046414, K77443]; Hungarian Ministry of Health : [ETT 078/2007]; EU [LSHG-CT-2004-502950]; European Community : [HPRN-CT-2004-504228] FX: We are grateful to Laszlo Tora, Alberto Ferrus, Sonja Georgieva, and : Kristen Johansen for kindly providing us Pol II, dADA3, dGCN5, and : JIL-1 antibodies. This work was supported by grants from the Hungarian : Science Fund (OTKA T046414 and K77443), the Hungarian Ministry of : Health (ETT 078/2007), and EU FP-6 (LSHG-CT-2004-502950). A. C. and C. : P. are European Community RTN Marie Curie research fellows supported by : the grant HPRN-CT-2004-504228. Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, Szeged 6726, Hungary Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary Cited By :6 Export Date: 31 August 2019 CODEN: CHROA Correspondence Address: Boros, I.; Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chemicals/CAS: lysine, 56-87-1, 6899-06-5, 70-54-2; polyethylene, 9002-88-4; serine, 56-45-1, 6898-95-9; Chromatin; DNA-Binding Proteins; Drosophila Proteins; Histone Acetyltransferases, 2.3.1.48; Histones; JIL-1 protein, Drosophila, 2.7.1.37; Lysine, 56-87-1; Pcaf protein, Drosophila, 2.3.1.48; Protein-Serine-Threonine Kinases, 2.7.11.1; Transcription Factors Funding details: ETT 078/2007 Funding details: HPRN-CT-2004-504228 Funding details: LSHG-CT-2004-502950, FP-6 Funding details: T046414, K77443 Funding text 1: Acknowledgments We are grateful to Laszlo Tora, Alberto Ferrus, Sonja Georgieva, and Kristen Johansen for kindly providing us Pol II, dADA3, dGCN5, and JIL-1 antibodies. This work was supported by grants from the Hungarian Science Fund (OTKA T046414 and K77443), the Hungarian Ministry of Health (ETT 078/2007), and EU FP-6 (LSHG-CT-2004-502950). A.C. and C. P. are European Community RTN Marie Curie research fellows supported by the grant HPRN-CT-2004-504228. Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, Szeged 6726, Hungary Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary Cited By :6 Export Date: 2 September 2019 CODEN: CHROA Correspondence Address: Boros, I.; Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chemicals/CAS: lysine, 56-87-1, 6899-06-5, 70-54-2; polyethylene, 9002-88-4; serine, 56-45-1, 6898-95-9; Chromatin; DNA-Binding Proteins; Drosophila Proteins; Histone Acetyltransferases, 2.3.1.48; Histones; JIL-1 protein, Drosophila, 2.7.1.37; Lysine, 56-87-1; Pcaf protein, Drosophila, 2.3.1.48; Protein-Serine-Threonine Kinases, 2.7.11.1; Transcription Factors Funding details: ETT 078/2007 Funding details: HPRN-CT-2004-504228 Funding details: LSHG-CT-2004-502950, FP-6 Funding details: T046414, K77443 Funding text 1: Acknowledgments We are grateful to Laszlo Tora, Alberto Ferrus, Sonja Georgieva, and Kristen Johansen for kindly providing us Pol II, dADA3, dGCN5, and JIL-1 antibodies. This work was supported by grants from the Hungarian Science Fund (OTKA T046414 and K77443), the Hungarian Ministry of Health (ETT 078/2007), and EU FP-6 (LSHG-CT-2004-502950). A.C. and C. P. are European Community RTN Marie Curie research fellows supported by the grant HPRN-CT-2004-504228. Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, Szeged 6726, Hungary Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary Cited By :6 Export Date: 12 February 2020 CODEN: CHROA Correspondence Address: Boros, I.; Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chemicals/CAS: lysine, 56-87-1, 6899-06-5, 70-54-2; polyethylene, 9002-88-4; serine, 56-45-1, 6898-95-9; Chromatin; DNA-Binding Proteins; Drosophila Proteins; Histone Acetyltransferases, 2.3.1.48; Histones; JIL-1 protein, Drosophila, 2.7.1.37; Lysine, 56-87-1; Pcaf protein, Drosophila, 2.3.1.48; Protein-Serine-Threonine Kinases, 2.7.11.1; Transcription Factors Funding details: ETT 078/2007 Funding details: HPRN-CT-2004-504228 Funding details: LSHG-CT-2004-502950, FP-6 Funding details: T046414, K77443 Funding text 1: Acknowledgments We are grateful to Laszlo Tora, Alberto Ferrus, Sonja Georgieva, and Kristen Johansen for kindly providing us Pol II, dADA3, dGCN5, and JIL-1 antibodies. This work was supported by grants from the Hungarian Science Fund (OTKA T046414 and K77443), the Hungarian Ministry of Health (ETT 078/2007), and EU FP-6 (LSHG-CT-2004-502950). A.C. and C. P. are European Community RTN Marie Curie research fellows supported by the grant HPRN-CT-2004-504228. Funding Agency and Grant Number: Hungarian Science Fund [T046414, K77443]; Hungarian Ministry of Health [ETT 078/2007]; EUEuropean Union (EU) [LSHG-CT-2004-502950]; European CommunityEuropean Community (EC) [HPRN-CT-2004-504228] Funding text: We are grateful to Laszlo Tora, Alberto Ferrus, Sonja Georgieva, and Kristen Johansen for kindly providing us Pol II, dADA3, dGCN5, and JIL-1 antibodies. This work was supported by grants from the Hungarian Science Fund (OTKA T046414 and K77443), the Hungarian Ministry of Health (ETT 078/2007), and EU FP-6 (LSHG-CT-2004-502950). A. C. and C. P. are European Community RTN Marie Curie research fellows supported by the grant HPRN-CT-2004-504228. Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, Szeged 6726, Hungary Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary Cited By :6 Export Date: 9 September 2020 CODEN: CHROA Correspondence Address: Boros, I.; Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chemicals/CAS: lysine, 56-87-1, 6899-06-5, 70-54-2; polyethylene, 9002-88-4; serine, 56-45-1, 6898-95-9; Chromatin; DNA-Binding Proteins; Drosophila Proteins; Histone Acetyltransferases, 2.3.1.48; Histones; JIL-1 protein, Drosophila, 2.7.1.37; Lysine, 56-87-1; Pcaf protein, Drosophila, 2.3.1.48; Protein-Serine-Threonine Kinases, 2.7.11.1; Transcription Factors Funding details: ETT 078/2007 Funding details: Hungarian Scientific Research Fund, OTKA, T046414, K77443 Funding details: LSHG-CT-2004-502950, HPRN-CT-2004-504228 Funding text 1: Acknowledgments We are grateful to Laszlo Tora, Alberto Ferrus, Sonja Georgieva, and Kristen Johansen for kindly providing us Pol II, dADA3, dGCN5, and JIL-1 antibodies. This work was supported by grants from the Hungarian Science Fund (OTKA T046414 and K77443), the Hungarian Ministry of Health (ETT 078/2007), and EU FP-6 (LSHG-CT-2004-502950). A.C. and C. P. are European Community RTN Marie Curie research fellows supported by the grant HPRN-CT-2004-504228. Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, Szeged 6726, Hungary Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary Cited By :6 Export Date: 17 January 2021 CODEN: CHROA Correspondence Address: Boros, I.; Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chemicals/CAS: lysine, 56-87-1, 6899-06-5, 70-54-2; polyethylene, 9002-88-4; serine, 56-45-1, 6898-95-9; Chromatin; DNA-Binding Proteins; Drosophila Proteins; Histone Acetyltransferases, 2.3.1.48; Histones; JIL-1 protein, Drosophila, 2.7.1.37; Lysine, 56-87-1; Pcaf protein, Drosophila, 2.3.1.48; Protein-Serine-Threonine Kinases, 2.7.11.1; Transcription Factors Funding details: ETT 078/2007 Funding details: Hungarian Scientific Research Fund, OTKA, T046414, K77443 Funding details: LSHG-CT-2004-502950, HPRN-CT-2004-504228 Funding text 1: Acknowledgments We are grateful to Laszlo Tora, Alberto Ferrus, Sonja Georgieva, and Kristen Johansen for kindly providing us Pol II, dADA3, dGCN5, and JIL-1 antibodies. This work was supported by grants from the Hungarian Science Fund (OTKA T046414 and K77443), the Hungarian Ministry of Health (ETT 078/2007), and EU FP-6 (LSHG-CT-2004-502950). A.C. and C. P. are European Community RTN Marie Curie research fellows supported by the grant HPRN-CT-2004-504228. Institute of Biochemistry, Biological Research Center, Temesvári krt. 62, Szeged 6726, Hungary Chromatin Research Group of HAS, Department of Biochemistry and Molecular Biology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary Cited By :6 Export Date: 14 March 2021 CODEN: CHROA Correspondence Address: Boros, I.; Chromatin Research Group of HAS, Közép fasor 52, 6726 Szeged, Hungary; email: borosi@bio.u-szeged.hu Chemicals/CAS: lysine, 56-87-1, 6899-06-5, 70-54-2; polyethylene, 9002-88-4; serine, 56-45-1, 6898-95-9; Chromatin; DNA-Binding Proteins; Drosophila Proteins; Histone Acetyltransferases, 2.3.1.48; Histones; JIL-1 protein, Drosophila, 2.7.1.37; Lysine, 56-87-1; Pcaf protein, Drosophila, 2.3.1.48; Protein-Serine-Threonine Kinases, 2.7.11.1; Transcription Factors Funding details: HPRN-CT-2004-504228, LSHG-CT-2004-502950 Funding details: ETT 078/2007 Funding details: Hungarian Scientific Research Fund, OTKA, K77443, T046414 Funding text 1: Acknowledgments We are grateful to Laszlo Tora, Alberto Ferrus, Sonja Georgieva, and Kristen Johansen for kindly providing us Pol II, dADA3, dGCN5, and JIL-1 antibodies. This work was supported by grants from the Hungarian Science Fund (OTKA T046414 and K77443), the Hungarian Ministry of Health (ETT 078/2007), and EU FP-6 (LSHG-CT-2004-502950). A.C. and C. P. are European Community RTN Marie Curie research fellows supported by the grant HPRN-CT-2004-504228. LA - English DB - MTMT ER -