TY - JOUR AU - Zsíros, Ottó AU - Nagy, Valéria AU - Párducz, Árpád AU - Nagy, Gergely AU - Ünnep, Renáta AU - El-Ramady, H. AU - Prokisch, József AU - Lisztes-Szabó, Zsuzsa AU - Fári, Miklós Gábor AU - Csajbók, József AU - Tóth, Szilvia Zita AU - Garab, Győző AU - Domokos-Szabolcsy, Éva TI - Effects of selenate and red Se-nanoparticles on the photosynthetic apparatus of Nicotiana tabacum JF - PHOTOSYNTHESIS RESEARCH J2 - PHOTOSYNTH RES VL - 139 PY - 2019 IS - 1-3 SP - 449 EP - 460 PG - 12 SN - 0166-8595 DO - 10.1007/s11120-018-0599-4 UR - https://m2.mtmt.hu/api/publication/30321753 ID - 30321753 N1 - Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, POB 521, Szeged, 6701, Hungary Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, POB 521, Szeged, 6701, Hungary Laboratory for Neutron Scattering, Paul Scherrer Institute, Villigen PSI, 5232, Switzerland Institute for Solid State Physics and Optics, Wigner Research Centre for Physics, Hungarian Academy of Sciences, POB 49, Budapest, 1525, Hungary Department of Soil and Water Sciences, Faculty of Agriculture, Kafrelsheikh Uni, Kafr El-Sheikh, 33516, Egypt Bio- and Environmental Enegetics Inst., Nano Food Lab, Debrecen University, Boszormenyi 138, Debrecen, 4032, Hungary Isotope Climatology and Environmental Research Centre, Institute for Nuclear Research, Hungarian Academy of Sciences, Debrecen, 4026, Hungary Department of Agricultural Botany, Plant Physiology and Biotechnology, University of Debrecen, Egyetem ter 1, Debrecen, 4032, Hungary Department of Crop Production and Applied Ecology, University of Debrecen, Boszormenyi 138, Debrecen, 4032, Hungary Department of Physics, Faculty of Science, Ostrava University, Chittussiho 10, Ostrava – Slezská Ostrava, 710 0, Czech Republic Cited By :9 Export Date: 19 January 2021 CODEN: PHRSD Correspondence Address: Domokos-Szabolcsy, É.; Department of Agricultural Botany, Plant Physiology and Biotechnology, University of Debrecen, Egyetem ter 1, Hungary; email: szabolcsy@agr.unideb.hu LA - English DB - MTMT ER - TY - JOUR AU - Hackler, L Jr AU - Ózsvári, Béla AU - Gyuris, M AU - Sipos, Péter AU - Fábián, Gabriella AU - Molnár, Eszter AU - Marton, Annamária AU - Faragó, Nóra AU - Mihály, József AU - Nagy, Lajos István AU - Szénási, Tibor AU - Diron, A AU - Párducz, Árpád AU - Kanizsai, Iván AU - Puskás, László TI - The Curcumin Analog C-150, Influencing NF-kappaB, UPR and Akt/Notch Pathways Has Potent Anticancer Activity In Vitro and In Vivo. JF - PLOS ONE J2 - PLOS ONE VL - 11 PY - 2016 IS - 3 PG - 16 SN - 1932-6203 DO - 10.1371/journal.pone.0149832 UR - https://m2.mtmt.hu/api/publication/3032868 ID - 3032868 N1 - AVIDIN Ltd., Szeged, Hungary Department of Pharmaceutical Technology, University of Szeged, Szeged, Hungary AVICOR Ltd., Szeged, Hungary Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Cited By :32 Export Date: 3 May 2022 CODEN: POLNC Chemicals/CAS: curcumin, 458-37-7; protein kinase B, 148640-14-6; Acrylamides; Antineoplastic Agents; C-150 curcumin; Curcumin; NF-kappa B; Proto-Oncogene Proteins c-akt; Receptors, Notch Tradenames: c 150 AB - C-150 a Mannich-type curcumin derivative, exhibited pronounced cytotoxic effects against eight glioma cell lines at micromolar concentrations. Inhibition of cell proliferation by C-150 was mediated by affecting multiple targets as confirmed at transcription and protein level. C-150 effectively reduced the transcription activation of NFkB, inhibited PKC-alpha which are constitutively over-expressed in glioblastoma. The effects of C-150 on the Akt/ Notch signaling were also demonstrated in a Drosophila tumorigenesis model. C-150 reduced the number of tumors in Drosophila with similar efficacy to mitoxantrone. In an in vivo orthotopic glioma model, C-150 significantly increased the median survival of treated nude rats compared to control animals. The multi-target action of C-150, and its preliminary in vivo efficacy would render this curcumin analogue as a potent clinical candidate against glioblastoma. LA - English DB - MTMT ER - TY - JOUR AU - Barabási, Beáta AU - Csondor, A AU - Martin-Pozas, T AU - Sanchez, AM AU - Antalffy, G AU - Siklós, László AU - Gomez-Pinedo, U AU - Párducz, Árpád AU - Hoyk, Zsófia TI - Effect of axotomy and 17beta-estradiol on P2X7 receptor expression pattern in the hypoglossal nucleus of ovariectomized mice. JF - NEUROSCIENCE J2 - NEUROSCIENCE VL - 319 PY - 2016 SP - 107 EP - 115 PG - 9 SN - 0306-4522 DO - 10.1016/j.neuroscience.2016.01.049 UR - https://m2.mtmt.hu/api/publication/3013488 ID - 3013488 N1 - Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, 6726, Hungary Instituto de Neurociencias, IdISSC, Hospital Clínico San Carlos, Universidad Complutense, c/ Prof Martín Lagos s/n, Madrid, 28040, Spain 3DHISTECH Ltd., Konkoly-Thege M. út 29-33, Budapest, 1121, Hungary Instituto de Neurociencias, IdISSC, Hospital Clínico San Carlos, Universidad Complutense, c/ Prof Martín Lagos s/n, Madrid, 28040, Spain Cited By :2 Export Date: 26 March 2020 CODEN: NRSCD Correspondence Address: Hoyk, Z.; Laboratory for Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Hungary; email: hoyk.zsofia@brc.mta.hu Chemicals/CAS: estradiol, 50-28-2; Estradiol; Receptors, Purinergic P2X7 Funding text 1: This work was supported by TÁMOP-4.2.2. A-11/1/KONV-2012-0052 and by Erasmus scholarship granted to Tamara Martín-Pozas and Adriana Marisol Pulupa Sánchez. Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged, 6726, Hungary Instituto de Neurociencias, IdISSC, Hospital Clínico San Carlos, Universidad Complutense, c/ Prof Martín Lagos s/n, Madrid, 28040, Spain 3DHISTECH Ltd., Konkoly-Thege M. út 29-33, Budapest, 1121, Hungary Instituto de Neurociencias, IdISSC, Hospital Clínico San Carlos, Universidad Complutense, c/ Prof Martín Lagos s/n, Madrid, 28040, Spain Cited By :2 Export Date: 28 April 2020 CODEN: NRSCD Correspondence Address: Hoyk, Z.; Laboratory for Molecular Neurobiology, Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Hungary; email: hoyk.zsofia@brc.mta.hu Chemicals/CAS: estradiol, 50-28-2; Estradiol; Receptors, Purinergic P2X7 Funding text 1: This work was supported by TÁMOP-4.2.2. A-11/1/KONV-2012-0052 and by Erasmus scholarship granted to Tamara Martín-Pozas and Adriana Marisol Pulupa Sánchez. AB - The objective of the study was to examine whether axotomy and 17beta-estradiol affects P2X7 receptor expression and distribution in the hypoglossal nucleus. The left hypoglossal nerve of ovariectomized mice was cut and animals received a single injection of 17beta-estradiol (25mug/100 g b. w. in 20% (2- hydroxypropyl) - beta- cyclodextrin) or vehicle one hour after axotomy. Mice were sacrificed on day 4 following surgery. The area fraction of P2X7 receptor immunoreactive structures and of CD11b immunolabeled microglia, P2X7 protein concentration, and the immunoreactivity pattern of estrogen receptor alpha / beta were analyzed on both sides of the hypoglossal nucleus. Following axotomy the area fraction of P2X7 immunoreactive neurons showed a decreasing tendency, while the area fraction of P2X7 immunolabeled microglia increased significantly on the axotomized side compared with the control side in mice injected with vehicle. In animals treated with 17beta-estradiol the decrease in area fraction of neural and the increase in area fraction of microglial P2X7 immunostaining on the axotomized side were significantly enhanced compared with animals injected with vehicle. The P2X7 immunoreactivity pattern on the control side of the nucleus remained unchanged after 17beta-estradiol injection. Semi-quantitative Western blots revealed no significant difference in P2X7 protein concentration comparing the axotomized side with the control side in either experimental group. The CD11b immunoreactive microglia area fraction increased significantly following axotomy, but was not affected by 17beta-estradiol. Neither estrogen receptor alpha, nor beta colocalized with CD11b. Our results suggest that axotomy induces cell-type specific changes in P2X7 receptor expression, which may be directly regulated by 17beta-estradiol through estrogen receptor alpha or beta in neurons, but not in activated microglia. LA - English DB - MTMT ER - TY - JOUR AU - Márkus, Róbert AU - Lerner, Zita AU - Honti, Viktor AU - Csordás, Gábor AU - Zsámboki, János AU - Cinege, Gyöngyi Ilona AU - Párducz, Árpád AU - Lukacsovich, Tamás AU - Kurucz, Judit Éva AU - Andó, István TI - Multinucleated Giant Hemocytes Are Effector Cells in Cell-Mediated Immune Responses of Drosophila JF - JOURNAL OF INNATE IMMUNITY J2 - J INNATE IMMUN VL - 7 PY - 2015 IS - 4 SP - 340 EP - 353 PG - 14 SN - 1662-811X DO - 10.1159/000369618 UR - https://m2.mtmt.hu/api/publication/2853634 ID - 2853634 N1 - Megjegyzés-25258522 Hiányzó Jelleg: 'JOUR\n\nArticle' Admin megjegyzés-25258522 tblcategory: (Category) ('JOUR\n\nArticle') #Jelleg AB - We identified and characterized a so far unrecognized cell type, dubbed the multinucleated giant hemocyte (MGH), in the ananassae subgroup of Drosophilidae. Here, we describe the functional and ultrastructural characteristics of this novel blood cell type as well as its characterization with a set of discriminative immunological markers. MGHs are encapsulating cells that isolate and kill the parasite without melanization. They share some properties with but differ considerably from lamellocytes, the encapsulating cells of Drosophila melanogaster, the broadly used model organism in studies of innate immunity. MGHs are nonproliferative effector cells that are derived from phagocytic cells of the sessile tissue and the circulation, but do not exhibit phagocytic activity. In contrast to lamellocytes, MGHs are gigantic cells with filamentous projections and contain many nuclei, which are the result of the fusion of several cells. Although the structure of lamellocytes and MGHs differ remarkably, their function in the elimination of parasites is similar, which is potentially the result of the convergent evolution of interactions between hosts and parasites in different geographic regions. MGHs are highly motile and share several features with mammalian multinucleated giant cells, a syncytium of macrophages formed during granulomatous inflammation. © 2015 S. Karger AG, Basel LA - English DB - MTMT ER - TY - JOUR AU - Hoyk, Zsófia AU - Csákvári, Eszter AU - Gyenes, Andrea AU - Siklós, László AU - Harada, N AU - Párducz, Árpád TI - Aromatase and estrogen receptor beta expression in the rat olfactory bulb: neuroestrogen action in the first relay station of the olfactory pathway? JF - ACTA NEUROBIOLOGIAE EXPERIMENTALIS J2 - ACTA NEUROBIOL EXP VL - 74 PY - 2014 IS - 1 SP - 1 EP - 14 PG - 14 SN - 0065-1400 UR - https://m2.mtmt.hu/api/publication/2515413 ID - 2515413 AB - The expression pattern of aromatase (ARO), the enzyme converting androgens to estrogens, was analyzed in the olfactory bulb of adult male rats and was compared with the distribution of estrogen receptor beta (ER beta), the main estrogen receptor isoform expressed in this brain region. A strong ARO immunolabeling obtained with a specificity tested antibody was observed in juxtaglomerular neurons of the glomerular layer and a weaker immunoreaction was detected in the mitral cell layer of the main olfactory bulb, while the granule cell layer of the main olfactory bulb as well as all layers in the accessory olfactory bulb showed faint immunolabeling. Fluorescence double labeling experiments revealed that ARO detected in juxtaglomerular neurons of the main olfactory bulb colocalized with tyrosine hydroxylase (TH) and glutamic acid decarboxylase 67 (GAD67), while no colocalization between ARO and the calcium binding proteins calretinin (CR) and calbindin (CB) was observed. Furthermore, the TH immunoreactive neurons expressed metabotropic glutamate receptor 1 (mGluR1) too. ER beta immunoreactivity, in contrast to ARO, was detected in all layers of both the main and accessory olfactory bulb. In the glomerular layer of the main olfactory bulb it was expressed in TH and GAD67 containing juxtaglomerular neurons, and it colocalized with CR, CB and even with glial fibrillary acidic protein too. Our morphological findings suggest that ARO expression is a novel feature of dopaminergic/GABAergic juxtaglomerular neurons in the adult rat main olfactory bulb, and raise the possibility that ARO activity may change in function of olfactory input via mGluR1. In situ estrogen production in the olfactory bulb in turn may modulate interglomerular circuits through ER beta. LA - English DB - MTMT ER - TY - JOUR AU - Dobrikova, AG AU - Racskóné Domonkos, Ildikó AU - Sözer, Özge AU - Laczkó-Dobos, Hajnalka AU - Kis, Mihály AU - Párducz, Árpád AU - Gombos, Zoltán AU - Apostolova, EL TI - Effect of partial or complete elimination of light-harvesting complexes on the surface electric properties and the functions of cyanobacterial photosynthetic membranes. JF - PHYSIOLOGIA PLANTARUM J2 - PHYSIOL PLANTARUM VL - 147 PY - 2013 IS - 2 SP - 248 EP - 260 PG - 13 SN - 0031-9317 DO - 10.1111/j.1399-3054.2012.01648.x UR - https://m2.mtmt.hu/api/publication/2170172 ID - 2170172 N1 - Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria Institute of Plant Biology, Biological Research Centre of the Hungarian Academy of Sciences, PO Box 521, Szeged, Hungary Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, PO Box 521, Szeged, Hungary Cited By :8 Export Date: 17 February 2020 CODEN: PHPLA Correspondence Address: Apostolova, E.L.; Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria; email: emya@bio21.bas.bg AB - Influence of the modification of the cyanobacterial light-harvesting complex [i.e. phycobilisomes (PBS)] on the surface electric properties and the functions of photosynthetic membranes was investigated. We used four PBS mutant strains of Synechocystis sp. PCC6803 as follows: PAL (PBS-less), CK (phycocyanin-less), BE (PSII-PBS-less) and PSI-less/apcE(-) (PSI-less with detached PBS). Modifications of the PBS content lead to changes in the cell morphology and surface electric properties of the thylakoid membranes as well as in their functions, such as photosynthetic oxygen-evolving activity, P700 kinetics and energy transfer between the pigment-protein complexes. Data reveal that the complete elimination of PBS in the PAL mutant causes a slight decrease in the electric dipole moments of the thylakoid membranes, whereas significant perturbations of the surface charges were registered in the membranes without assembled PBS-PSII macrocomplex (BE mutant) or PSI complex (PSI-less mutant). These observations correlate with the detected alterations in the membrane structural organization. Using a polarographic oxygen rate electrode, we showed that the ratio of the fast to the slow oxygen-evolving PSII centers depends on the partial or complete elimination of light-harvesting complexes, as the slow operating PSII centers dominate in the PBS-less mutant and in the mutant with detached PBS. LA - English DB - MTMT ER - TY - JOUR AU - Sajben-Nagy, Enikő Ilona AU - Maróti, Gergely AU - Kredics, László AU - Horváth, Balázs AU - Párducz, Árpád AU - Vágvölgyi, Csaba AU - Manczinger, László TI - Isolation of new Pseudomonas tolaasii bacteriophages and genomic inverstigation of the lytic phage BF7 JF - FEMS MICROBIOLOGY LETTERS J2 - FEMS MICROBIOL LETT VL - 332 PY - 2012 IS - 2 SP - 162 EP - 169 PG - 8 SN - 0378-1097 DO - 10.1111/j.1574-6968.2012.02592.x UR - https://m2.mtmt.hu/api/publication/1892107 ID - 1892107 N1 - Research Letter LA - English DB - MTMT ER - TY - JOUR AU - Gyenes, Andrea AU - Hoyk, Zsófia AU - Csákvári, Eszter AU - Siklós, László AU - Párducz, Árpád TI - 17 beta-ESTRADIOL ATTENUATES INJURY-INDUCED MICROGLIA ACTIVATION IN THE OCULOMOTOR NUCLEUS JF - NEUROSCIENCE J2 - NEUROSCIENCE VL - 171 PY - 2010 IS - 3 SP - 677 EP - 682 PG - 6 SN - 0306-4522 DO - 10.1016/j.neuroscience.2010.09.033 UR - https://m2.mtmt.hu/api/publication/1921486 ID - 1921486 N1 - Megjegyzés-22191353 DI: 10.1016/j.neuroscience.2010.09.033 Cited By :12 Export Date: 2 June 2020 CODEN: NRSCD Correspondence Address: Parducz, A.; Institute of Biophysics, Biological Research Center, Temesvári körút 62, H-6726, Szeged, Hungary; email: parducz@brc.hu Chemicals/CAS: estradiol, 50-28-2; Estradiol, 50-28-2; Neuroprotective Agents Funding details: GVOP-3.2.1 Funding text 1: The study was supported by the Hungarian National Office for Research and Technology ( GVOP-3.2.1. 2004-04-0052/3.0 , OM-00103/2008 GLINOLID and TÁMOP-4.2.2/08/1/2008-0002 ), OTKA K075954 , TeT ES-5/2008 and Bolyai Fellowship (E. Cs.) AB - Recent studies provide increasing data indicating the prominent role of estrogens in protecting the nervous system against the noxious consequences of nerve injury. It is also clear that in the process of nerve injury and recovery not only the neurons, but the glial cells are also involved and they are important components of the protective mechanisms. In the present article the effect of 17 beta-estradiol on injury-induced microglia activation was studied in an animal model. Peripheral axotomy of the oculomotor neurons was achieved by the removal of the right eyeball including the extraocular muscles of ovariectomized adult mice. The time course and the extent of microglia activation was followed by the unbiased morphometric analysis of CD11b immunoreactive structures within the oculomotor nucleus. The first sign of microglia activation appeared after 24 h following injury, the maximal effect was found on the fourth day. In ovariectomized females hormone treatment (daily injection of 17 beta-estradiol, 5 mu g/100 g b.w.) decreased significantly the microglia reaction at postoperative day 4. Our results show that microglia response to nerve injury is affected by estradiol, that is these cells may mediate some of the hormonal effects and may contribute to protective mechanisms resulting in the structural and Functional recovery of the nervous system. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved. LA - English DB - MTMT ER - TY - JOUR AU - Magnaghi, V AU - Párducz, Árpád AU - Frasca, A AU - Ballabio, M AU - Procacci, P AU - Racagni, G AU - Bonanno, G AU - Fumagalli, F TI - Gaba synthesis in schwann cells is induced by the neuroactive steroid allopregnanolone JF - JOURNAL OF NEUROCHEMISTRY J2 - J NEUROCHEM VL - 112 PY - 2010 IS - 4 SP - 980 EP - 990 PG - 11 SN - 0022-3042 DO - 10.1111/j.1471-4159.2009.06512.x UR - https://m2.mtmt.hu/api/publication/1920974 ID - 1920974 AB - Recent evidence showed that neurotransmitters are synthesised in glial cells, such as the Schwann cells, which form myelin sheaths in the PNS. While the presence of GABA type A (GABA-A) receptors has been previously demonstrated in these cells, the evidence of GABA synthesis remained still elusive. In an attempt to demonstrate the presence of GABA in rat Schwann cells, we adopted a strategy, using several integrated neurochemical, molecular as well as immunocytochemical approaches. We first demonstrated the presence of glutamic acid decarboxylase of 67 kDa (GAD67) in Schwann cells, a crucial enzyme of the GABA synthesis mechanism. Second, we demonstrated that GABA is synthesized and localized in Schwann cells. As the third step we showed that allopregnanolone (10 nM), a potent allosteric modulator of GABA-A receptors, stimulates GABA synthesis through increased levels of GAD67 in Schwann cells. Analysis of intracellular signalling mechanisms revealed that the protein kinase A pathway, through enhanced cAMP levels and cAMP response element binding protein phosphorylation, modulates the allosteric action of allopregnanolone at the GABA-A receptor in Schwann cells. Our findings are the first to demonstrate that this GABA mechanism is active in Schwann cells thus establishing new potential therapeutic targets to control Schwann cell biology, which may prove useful in the treatment of several neurodegenerative disorders. LA - English DB - MTMT ER - TY - JOUR AU - Hajszán, Tibor AU - Szigeti-Buck, K AU - Sallam, NL AU - Bober, J AU - Párducz, Árpád AU - MacLusky, NJ AU - Leranth, C AU - Duman, RS TI - Effects of estradiol on learned helplessness and associated remodeling of hippocampal spine synapses in female rats JF - BIOLOGICAL PSYCHIATRY J2 - BIOL PSYCHIAT VL - 67 PY - 2010 IS - 2 SP - 168 EP - 174 PG - 7 SN - 0006-3223 DO - 10.1016/j.biopsych.2009.08.017 UR - https://m2.mtmt.hu/api/publication/1920972 ID - 1920972 AB - Background: Despite the fact that women are twice as likely to develop depression as men, our understanding of depression neurobiology in female subjects is limited. We have recently reported in male rats that development of helpless behavior is associated with a severe loss of hippocampal spine synapses, which is reversed by treatment with the antidepressant desipramine. Considering that estradiol has a hippocampal synaptogenic effect similar to those of antidepressants, the presence of estradiol during the female reproductive life might influence behavioral and synaptic responses to stress and depression. Methods: With electron microscopic stereology, we analyzed hippocampal spine synapses in association with helpless behavior in ovariectomized female rats (n = 70), under different conditions of estradiol exposure. Results: Stress induced an acute and persistent loss of hippocampal spine synapses, whereas subchronic treatment with desipramine reversed the stress-induced synaptic loss. Estradiol supplementation given either before stress or before escape testing of nonstressed animals increased the number of hippocampal spine synapses. Correlation analysis demonstrated a statistically significant negative correlation between the severity of helpless behavior and hippocampal spine synapse numbers. Conclusions: These findings suggest that hippocampal spine synapse remodeling might be a critical factor underlying learned helplessness and, possibly, the neurobiology of depression. LA - English DB - MTMT ER -