@article{MTMT:30321753, title = {Effects of selenate and red Se-nanoparticles on the photosynthetic apparatus of Nicotiana tabacum}, url = {https://m2.mtmt.hu/api/publication/30321753}, author = {Zsíros, Ottó and Nagy, Valéria and Párducz, Árpád and Nagy, Gergely and Ünnep, Renáta and El-Ramady, H. and Prokisch, József and Lisztes-Szabó, Zsuzsa and Fári, Miklós Gábor and Csajbók, József and Tóth, Szilvia Zita and Garab, Győző and Domokos-Szabolcsy, Éva}, doi = {10.1007/s11120-018-0599-4}, journal-iso = {PHOTOSYNTH RES}, journal = {PHOTOSYNTHESIS RESEARCH}, volume = {139}, unique-id = {30321753}, issn = {0166-8595}, year = {2019}, eissn = {1573-5079}, pages = {449-460}, orcid-numbers = {Nagy, Valéria/0000-0001-7105-3546; Lisztes-Szabó, Zsuzsa/0000-0002-6322-8542; Csajbók, József/0000-0003-0281-2590} } @article{MTMT:3032868, title = {The Curcumin Analog C-150, Influencing NF-kappaB, UPR and Akt/Notch Pathways Has Potent Anticancer Activity In Vitro and In Vivo.}, url = {https://m2.mtmt.hu/api/publication/3032868}, author = {Hackler, L Jr and Ózsvári, Béla and Gyuris, M and Sipos, Péter and Fábián, Gabriella and Molnár, Eszter and Marton, Annamária and Faragó, Nóra and Mihály, József and Nagy, Lajos István and Szénási, Tibor and Diron, A and Párducz, Árpád and Kanizsai, Iván and Puskás, László}, doi = {10.1371/journal.pone.0149832}, journal-iso = {PLOS ONE}, journal = {PLOS ONE}, volume = {11}, unique-id = {3032868}, issn = {1932-6203}, abstract = {C-150 a Mannich-type curcumin derivative, exhibited pronounced cytotoxic effects against eight glioma cell lines at micromolar concentrations. Inhibition of cell proliferation by C-150 was mediated by affecting multiple targets as confirmed at transcription and protein level. C-150 effectively reduced the transcription activation of NFkB, inhibited PKC-alpha which are constitutively over-expressed in glioblastoma. The effects of C-150 on the Akt/ Notch signaling were also demonstrated in a Drosophila tumorigenesis model. C-150 reduced the number of tumors in Drosophila with similar efficacy to mitoxantrone. In an in vivo orthotopic glioma model, C-150 significantly increased the median survival of treated nude rats compared to control animals. The multi-target action of C-150, and its preliminary in vivo efficacy would render this curcumin analogue as a potent clinical candidate against glioblastoma.}, year = {2016}, eissn = {1932-6203}, orcid-numbers = {Ózsvári, Béla/0000-0001-9108-354X; Fábián, Gabriella/0000-0002-2323-4948; Szénási, Tibor/0000-0002-8405-0798} } @article{MTMT:3013488, title = {Effect of axotomy and 17beta-estradiol on P2X7 receptor expression pattern in the hypoglossal nucleus of ovariectomized mice.}, url = {https://m2.mtmt.hu/api/publication/3013488}, author = {Barabási, Beáta and Csondor, A and Martin-Pozas, T and Sanchez, AM and Antalffy, G and Siklós, László and Gomez-Pinedo, U and Párducz, Árpád and Hoyk, Zsófia}, doi = {10.1016/j.neuroscience.2016.01.049}, journal-iso = {NEUROSCIENCE}, journal = {NEUROSCIENCE}, volume = {319}, unique-id = {3013488}, issn = {0306-4522}, abstract = {The objective of the study was to examine whether axotomy and 17beta-estradiol affects P2X7 receptor expression and distribution in the hypoglossal nucleus. The left hypoglossal nerve of ovariectomized mice was cut and animals received a single injection of 17beta-estradiol (25mug/100 g b. w. in 20% (2- hydroxypropyl) - beta- cyclodextrin) or vehicle one hour after axotomy. Mice were sacrificed on day 4 following surgery. The area fraction of P2X7 receptor immunoreactive structures and of CD11b immunolabeled microglia, P2X7 protein concentration, and the immunoreactivity pattern of estrogen receptor alpha / beta were analyzed on both sides of the hypoglossal nucleus. Following axotomy the area fraction of P2X7 immunoreactive neurons showed a decreasing tendency, while the area fraction of P2X7 immunolabeled microglia increased significantly on the axotomized side compared with the control side in mice injected with vehicle. In animals treated with 17beta-estradiol the decrease in area fraction of neural and the increase in area fraction of microglial P2X7 immunostaining on the axotomized side were significantly enhanced compared with animals injected with vehicle. The P2X7 immunoreactivity pattern on the control side of the nucleus remained unchanged after 17beta-estradiol injection. Semi-quantitative Western blots revealed no significant difference in P2X7 protein concentration comparing the axotomized side with the control side in either experimental group. The CD11b immunoreactive microglia area fraction increased significantly following axotomy, but was not affected by 17beta-estradiol. Neither estrogen receptor alpha, nor beta colocalized with CD11b. Our results suggest that axotomy induces cell-type specific changes in P2X7 receptor expression, which may be directly regulated by 17beta-estradiol through estrogen receptor alpha or beta in neurons, but not in activated microglia.}, year = {2016}, eissn = {1873-7544}, pages = {107-115} } @article{MTMT:2853634, title = {Multinucleated Giant Hemocytes Are Effector Cells in Cell-Mediated Immune Responses of Drosophila}, url = {https://m2.mtmt.hu/api/publication/2853634}, author = {Márkus, Róbert and Lerner, Zita and Honti, Viktor and Csordás, Gábor and Zsámboki, János and Cinege, Gyöngyi Ilona and Párducz, Árpád and Lukacsovich, Tamás and Kurucz, Judit Éva and Andó, István}, doi = {10.1159/000369618}, journal-iso = {J INNATE IMMUN}, journal = {JOURNAL OF INNATE IMMUNITY}, volume = {7}, unique-id = {2853634}, issn = {1662-811X}, abstract = {We identified and characterized a so far unrecognized cell type, dubbed the multinucleated giant hemocyte (MGH), in the ananassae subgroup of Drosophilidae. Here, we describe the functional and ultrastructural characteristics of this novel blood cell type as well as its characterization with a set of discriminative immunological markers. MGHs are encapsulating cells that isolate and kill the parasite without melanization. They share some properties with but differ considerably from lamellocytes, the encapsulating cells of Drosophila melanogaster, the broadly used model organism in studies of innate immunity. MGHs are nonproliferative effector cells that are derived from phagocytic cells of the sessile tissue and the circulation, but do not exhibit phagocytic activity. In contrast to lamellocytes, MGHs are gigantic cells with filamentous projections and contain many nuclei, which are the result of the fusion of several cells. Although the structure of lamellocytes and MGHs differ remarkably, their function in the elimination of parasites is similar, which is potentially the result of the convergent evolution of interactions between hosts and parasites in different geographic regions. MGHs are highly motile and share several features with mammalian multinucleated giant cells, a syncytium of macrophages formed during granulomatous inflammation. © 2015 S. Karger AG, Basel}, year = {2015}, eissn = {1662-8128}, pages = {340-353}, orcid-numbers = {Csordás, Gábor/0000-0001-6871-6839; Andó, István/0000-0002-4648-9396} } @article{MTMT:2515413, title = {Aromatase and estrogen receptor beta expression in the rat olfactory bulb: neuroestrogen action in the first relay station of the olfactory pathway?}, url = {https://m2.mtmt.hu/api/publication/2515413}, author = {Hoyk, Zsófia and Csákvári, Eszter and Gyenes, Andrea and Siklós, László and Harada, N and Párducz, Árpád}, journal-iso = {ACTA NEUROBIOL EXP}, journal = {ACTA NEUROBIOLOGIAE EXPERIMENTALIS}, volume = {74}, unique-id = {2515413}, issn = {0065-1400}, abstract = {The expression pattern of aromatase (ARO), the enzyme converting androgens to estrogens, was analyzed in the olfactory bulb of adult male rats and was compared with the distribution of estrogen receptor beta (ER beta), the main estrogen receptor isoform expressed in this brain region. A strong ARO immunolabeling obtained with a specificity tested antibody was observed in juxtaglomerular neurons of the glomerular layer and a weaker immunoreaction was detected in the mitral cell layer of the main olfactory bulb, while the granule cell layer of the main olfactory bulb as well as all layers in the accessory olfactory bulb showed faint immunolabeling. Fluorescence double labeling experiments revealed that ARO detected in juxtaglomerular neurons of the main olfactory bulb colocalized with tyrosine hydroxylase (TH) and glutamic acid decarboxylase 67 (GAD67), while no colocalization between ARO and the calcium binding proteins calretinin (CR) and calbindin (CB) was observed. Furthermore, the TH immunoreactive neurons expressed metabotropic glutamate receptor 1 (mGluR1) too. ER beta immunoreactivity, in contrast to ARO, was detected in all layers of both the main and accessory olfactory bulb. In the glomerular layer of the main olfactory bulb it was expressed in TH and GAD67 containing juxtaglomerular neurons, and it colocalized with CR, CB and even with glial fibrillary acidic protein too. Our morphological findings suggest that ARO expression is a novel feature of dopaminergic/GABAergic juxtaglomerular neurons in the adult rat main olfactory bulb, and raise the possibility that ARO activity may change in function of olfactory input via mGluR1. In situ estrogen production in the olfactory bulb in turn may modulate interglomerular circuits through ER beta.}, year = {2014}, eissn = {1689-0035}, pages = {1-14} } @article{MTMT:2170172, title = {Effect of partial or complete elimination of light-harvesting complexes on the surface electric properties and the functions of cyanobacterial photosynthetic membranes.}, url = {https://m2.mtmt.hu/api/publication/2170172}, author = {Dobrikova, AG and Racskóné Domonkos, Ildikó and Sözer, Özge and Laczkó-Dobos, Hajnalka and Kis, Mihály and Párducz, Árpád and Gombos, Zoltán and Apostolova, EL}, doi = {10.1111/j.1399-3054.2012.01648.x}, journal-iso = {PHYSIOL PLANTARUM}, journal = {PHYSIOLOGIA PLANTARUM}, volume = {147}, unique-id = {2170172}, issn = {0031-9317}, abstract = {Influence of the modification of the cyanobacterial light-harvesting complex [i.e. phycobilisomes (PBS)] on the surface electric properties and the functions of photosynthetic membranes was investigated. We used four PBS mutant strains of Synechocystis sp. PCC6803 as follows: PAL (PBS-less), CK (phycocyanin-less), BE (PSII-PBS-less) and PSI-less/apcE(-) (PSI-less with detached PBS). Modifications of the PBS content lead to changes in the cell morphology and surface electric properties of the thylakoid membranes as well as in their functions, such as photosynthetic oxygen-evolving activity, P700 kinetics and energy transfer between the pigment-protein complexes. Data reveal that the complete elimination of PBS in the PAL mutant causes a slight decrease in the electric dipole moments of the thylakoid membranes, whereas significant perturbations of the surface charges were registered in the membranes without assembled PBS-PSII macrocomplex (BE mutant) or PSI complex (PSI-less mutant). These observations correlate with the detected alterations in the membrane structural organization. Using a polarographic oxygen rate electrode, we showed that the ratio of the fast to the slow oxygen-evolving PSII centers depends on the partial or complete elimination of light-harvesting complexes, as the slow operating PSII centers dominate in the PBS-less mutant and in the mutant with detached PBS.}, year = {2013}, eissn = {1399-3054}, pages = {248-260} } @article{MTMT:1892107, title = {Isolation of new Pseudomonas tolaasii bacteriophages and genomic inverstigation of the lytic phage BF7}, url = {https://m2.mtmt.hu/api/publication/1892107}, author = {Sajben-Nagy, Enikő Ilona and Maróti, Gergely and Kredics, László and Horváth, Balázs and Párducz, Árpád and Vágvölgyi, Csaba and Manczinger, László}, doi = {10.1111/j.1574-6968.2012.02592.x}, journal-iso = {FEMS MICROBIOL LETT}, journal = {FEMS MICROBIOLOGY LETTERS}, volume = {332}, unique-id = {1892107}, issn = {0378-1097}, year = {2012}, eissn = {1574-6968}, pages = {162-169}, orcid-numbers = {Maróti, Gergely/0000-0002-3705-0461; Kredics, László/0000-0002-8837-3973; Vágvölgyi, Csaba/0000-0003-0009-7773; Manczinger, László/0000-0003-1031-7522} } @article{MTMT:1921486, title = {17 beta-ESTRADIOL ATTENUATES INJURY-INDUCED MICROGLIA ACTIVATION IN THE OCULOMOTOR NUCLEUS}, url = {https://m2.mtmt.hu/api/publication/1921486}, author = {Gyenes, Andrea and Hoyk, Zsófia and Csákvári, Eszter and Siklós, László and Párducz, Árpád}, doi = {10.1016/j.neuroscience.2010.09.033}, journal-iso = {NEUROSCIENCE}, journal = {NEUROSCIENCE}, volume = {171}, unique-id = {1921486}, issn = {0306-4522}, abstract = {Recent studies provide increasing data indicating the prominent role of estrogens in protecting the nervous system against the noxious consequences of nerve injury. It is also clear that in the process of nerve injury and recovery not only the neurons, but the glial cells are also involved and they are important components of the protective mechanisms. In the present article the effect of 17 beta-estradiol on injury-induced microglia activation was studied in an animal model. Peripheral axotomy of the oculomotor neurons was achieved by the removal of the right eyeball including the extraocular muscles of ovariectomized adult mice. The time course and the extent of microglia activation was followed by the unbiased morphometric analysis of CD11b immunoreactive structures within the oculomotor nucleus. The first sign of microglia activation appeared after 24 h following injury, the maximal effect was found on the fourth day. In ovariectomized females hormone treatment (daily injection of 17 beta-estradiol, 5 mu g/100 g b.w.) decreased significantly the microglia reaction at postoperative day 4. Our results show that microglia response to nerve injury is affected by estradiol, that is these cells may mediate some of the hormonal effects and may contribute to protective mechanisms resulting in the structural and Functional recovery of the nervous system. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.}, year = {2010}, eissn = {1873-7544}, pages = {677-682} } @article{MTMT:1920974, title = {Gaba synthesis in schwann cells is induced by the neuroactive steroid allopregnanolone}, url = {https://m2.mtmt.hu/api/publication/1920974}, author = {Magnaghi, V and Párducz, Árpád and Frasca, A and Ballabio, M and Procacci, P and Racagni, G and Bonanno, G and Fumagalli, F}, doi = {10.1111/j.1471-4159.2009.06512.x}, journal-iso = {J NEUROCHEM}, journal = {JOURNAL OF NEUROCHEMISTRY}, volume = {112}, unique-id = {1920974}, issn = {0022-3042}, abstract = {Recent evidence showed that neurotransmitters are synthesised in glial cells, such as the Schwann cells, which form myelin sheaths in the PNS. While the presence of GABA type A (GABA-A) receptors has been previously demonstrated in these cells, the evidence of GABA synthesis remained still elusive. In an attempt to demonstrate the presence of GABA in rat Schwann cells, we adopted a strategy, using several integrated neurochemical, molecular as well as immunocytochemical approaches. We first demonstrated the presence of glutamic acid decarboxylase of 67 kDa (GAD67) in Schwann cells, a crucial enzyme of the GABA synthesis mechanism. Second, we demonstrated that GABA is synthesized and localized in Schwann cells. As the third step we showed that allopregnanolone (10 nM), a potent allosteric modulator of GABA-A receptors, stimulates GABA synthesis through increased levels of GAD67 in Schwann cells. Analysis of intracellular signalling mechanisms revealed that the protein kinase A pathway, through enhanced cAMP levels and cAMP response element binding protein phosphorylation, modulates the allosteric action of allopregnanolone at the GABA-A receptor in Schwann cells. Our findings are the first to demonstrate that this GABA mechanism is active in Schwann cells thus establishing new potential therapeutic targets to control Schwann cell biology, which may prove useful in the treatment of several neurodegenerative disorders.}, year = {2010}, eissn = {1471-4159}, pages = {980-990} } @article{MTMT:1920972, title = {Effects of estradiol on learned helplessness and associated remodeling of hippocampal spine synapses in female rats}, url = {https://m2.mtmt.hu/api/publication/1920972}, author = {Hajszán, Tibor and Szigeti-Buck, K and Sallam, NL and Bober, J and Párducz, Árpád and MacLusky, NJ and Leranth, C and Duman, RS}, doi = {10.1016/j.biopsych.2009.08.017}, journal-iso = {BIOL PSYCHIAT}, journal = {BIOLOGICAL PSYCHIATRY}, volume = {67}, unique-id = {1920972}, issn = {0006-3223}, abstract = {Background: Despite the fact that women are twice as likely to develop depression as men, our understanding of depression neurobiology in female subjects is limited. We have recently reported in male rats that development of helpless behavior is associated with a severe loss of hippocampal spine synapses, which is reversed by treatment with the antidepressant desipramine. Considering that estradiol has a hippocampal synaptogenic effect similar to those of antidepressants, the presence of estradiol during the female reproductive life might influence behavioral and synaptic responses to stress and depression. Methods: With electron microscopic stereology, we analyzed hippocampal spine synapses in association with helpless behavior in ovariectomized female rats (n = 70), under different conditions of estradiol exposure. Results: Stress induced an acute and persistent loss of hippocampal spine synapses, whereas subchronic treatment with desipramine reversed the stress-induced synaptic loss. Estradiol supplementation given either before stress or before escape testing of nonstressed animals increased the number of hippocampal spine synapses. Correlation analysis demonstrated a statistically significant negative correlation between the severity of helpless behavior and hippocampal spine synapse numbers. Conclusions: These findings suggest that hippocampal spine synapse remodeling might be a critical factor underlying learned helplessness and, possibly, the neurobiology of depression.}, year = {2010}, eissn = {1873-2402}, pages = {168-174} }