@article{MTMT:36306037, title = {Developmental remodelling of Drosophila flight muscle sarcomeres: A scaled myofilament lattice model based on multiscale morphometrics}, url = {https://m2.mtmt.hu/api/publication/36306037}, author = {Görög, Péter and Novák, Tibor and Polgár, Tamás Ferenc and Bíró, Péter and Gutheil, A. and Kozma, C. and Gajdos, Tamás and Tóth, Krisztina and Tóth, Alexandra Vivien and Erdélyi, Miklós and Mihály, József and Szikora, Szilárd}, doi = {10.1098/rsob.250182}, journal-iso = {OPEN BIOL}, journal = {OPEN BIOLOGY}, volume = {15}, unique-id = {36306037}, abstract = {The indirect flight muscle is a widely used model for studying sarcomere structure and muscle development due to its extremely regular architecture. Nevertheless, precise measurement of the basic sarcomeric parameters remains a challenge even in this greatly ordered tissue. In this study, we identified several factors affecting measurement reliability and developed a software tool for precise, high-throughput measurement of sarcomere length and myofibril width. The accuracy of this new tool was validated against simulated images and blinded manual measurements. To extend the scope of this morphometric analysis to the sub-sarcomeric scale, we used electron and super-resolution microscopy to quantify myofilament number and filament length during myofibrillogenesis. Our findings revealed the dynamics of thin and thick filament elongation, as well as the addition of myofilaments at the sarcomere periphery during myofibrillogenesis. We precisely measured the dimensions of the Z-disc, I-band and H-zone during development, enabling us to construct refined models of sarcomere growth at the level of individual myofilaments, providing a spatial framework for interpreting nanoscopic localization data. These models deepen our understanding of sarcomere growth and lay the groundwork for future studies on the molecular mechanisms driving myofilament elongation and assembly. © 2025 The Authors.}, keywords = {Male; ARTICLE; MICROSCOPY; DROSOPHILA; DROSOPHILA; Software; controlled study; nonhuman; morphometry; simulation; Reliability; ELECTRON; muscle development; muscle fibril; thick filament; sarcomere; sarcomere; Flight muscle; Flight muscle; Indirect flight muscle; Nanoscopy; Sarcomere length; thin filament; thin filament; myofilament; tenotome}, year = {2025}, eissn = {2046-2441}, orcid-numbers = {Görög, Péter/0000-0003-4153-0385; Novák, Tibor/0000-0003-0756-6851; Polgár, Tamás Ferenc/0000-0002-6287-1093; Bíró, Péter/0000-0001-8103-6248; Gajdos, Tamás/0000-0002-5288-4659; Erdélyi, Miklós/0000-0002-9501-5752} } @mastersthesis{MTMT:37067914, title = {Flightless-I and Drosophila dLRRFIP2 work together to regulate radial growth of the sarcomeres [Flightless-I és a Drosophila dLRRFIP2 fehérjék együttműködése a szarkomerek radiális növekedésének szabályozásában]}, url = {https://m2.mtmt.hu/api/publication/37067914}, author = {Görög, Péter}, doi = {10.14232/phd.12890}, publisher = {Universití of Szeged}, unique-id = {37067914}, year = {2025}, orcid-numbers = {Görög, Péter/0000-0003-4153-0385} } @article{MTMT:32799759, title = {Molecular Dissection of DAAM Function during Axon Growth in Drosophila Embryonic Neurons}, url = {https://m2.mtmt.hu/api/publication/32799759}, author = {Földi, István and Tóth, Krisztina and Gombos, Rita I and Gaszler, Péter and Görög, Péter and Zygouras, Ioannis and Bugyi, Beáta and Mihály, József}, doi = {10.3390/cells11091487}, journal-iso = {CELLS-BASEL}, journal = {CELLS}, volume = {11}, unique-id = {32799759}, abstract = {Axonal growth is mediated by coordinated changes of the actin and microtubule (MT) cytoskeleton. Ample evidence suggests that members of the formin protein family are involved in the coordination of these cytoskeletal rearrangements, but the molecular mechanisms of the formin-dependent actin–microtubule crosstalk remains largely elusive. Of the six Drosophila formins, DAAM was shown to play a pivotal role during axonal growth in all stages of nervous system development, while FRL was implicated in axonal development in the adult brain. Here, we aimed to investigate the potentially redundant function of these two formins, and we attempted to clarify which molecular activities are important for axonal growth. We used a combination of genetic analyses, cellular assays and biochemical approaches to demonstrate that the actin-processing activity of DAAM is indispensable for axonal growth in every developmental condition. In addition, we identified a novel MT-binding motif within the FH2 domain of DAAM, which is required for proper growth and guidance of the mushroom body axons, while being dispensable during embryonic axon development. Together, these data suggest that DAAM is the predominant formin during axonal growth in Drosophila, and highlight the contribution of multiple formin-mediated mechanisms in cytoskeleton coordination during axonal growth.}, year = {2022}, eissn = {2073-4409}, orcid-numbers = {Görög, Péter/0000-0003-4153-0385; Bugyi, Beáta/0000-0003-4181-3633} } @article{MTMT:32840276, title = {The mechanisms of thin filament assembly and length regulation in muscles}, url = {https://m2.mtmt.hu/api/publication/32840276}, author = {Szikora, Szilárd and Görög, Péter and Mihály, József}, doi = {10.3390/ijms23105306}, journal-iso = {INT J MOL SCI}, journal = {INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}, volume = {23}, unique-id = {32840276}, issn = {1661-6596}, abstract = {The actin containing tropomyosin and troponin decorated thin filaments form one of the crucial components of the contractile apparatus in muscles. The thin filaments are organized into densely packed lattices interdigitated with myosin-based thick filaments. The crossbridge interactions between these myofilaments drive muscle contraction, and the degree of myofilament overlap is a key factor of contractile force determination. As such, the optimal length of the thin filaments is critical for efficient activity, therefore, this parameter is precisely controlled according to the workload of a given muscle. Thin filament length is thought to be regulated by two major, but only partially understood mechanisms: it is set by (i) factors that mediate the assembly of filaments from monomers and catalyze their elongation, and (ii) by factors that specify their length and uniformity. Mutations affecting these factors can alter the length of thin filaments, and in human cases, many of them are linked to debilitating diseases such as nemaline myopathy and dilated cardiomyopathy.}, year = {2022}, eissn = {1422-0067}, orcid-numbers = {Görög, Péter/0000-0003-4153-0385} } @article{MTMT:32153073, title = {Drosophila models rediscovered with super-resolution microscopy}, url = {https://m2.mtmt.hu/api/publication/32153073}, author = {Szikora, Szilárd and Görög, Péter and Kozma, Csaba and Mihály, József}, doi = {10.3390/cells10081924}, journal-iso = {CELLS-BASEL}, journal = {CELLS}, volume = {10}, unique-id = {32153073}, abstract = {With the advent of super-resolution microscopy, we gained a powerful toolbox to bridge the gap between the cellular- and molecular-level analysis of living organisms. Although nanoscopy is broadly applicable, classical model organisms, such as fruit flies, worms and mice, remained the leading subjects because combining the strength of sophisticated genetics, biochemistry and electrophysiology with the unparalleled resolution provided by super-resolution imaging appears as one of the most efficient approaches to understanding the basic cell biological questions and the molecular complexity of life. Here, we summarize the major nanoscopic techniques and illustrate how these approaches were used in Drosophila model systems to revisit a series of well-known cell biological phenomena. These investigations clearly demonstrate that instead of simply achieving an improvement in image quality, nanoscopy goes far beyond with its immense potential to discover novel structural and mechanistic aspects. With the examples of synaptic active zones, centrosomes and sarcomeres, we will explain the instrumental role of super-resolution imaging pioneered in Drosophila in understanding fundamental subcellular constituents.}, year = {2021}, eissn = {2073-4409}, orcid-numbers = {Görög, Péter/0000-0003-4153-0385} }